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    PRODUCTION OF BETA-LACTAM ANTIBIOTICS
    2.
    发明申请
    PRODUCTION OF BETA-LACTAM ANTIBIOTICS 有权
    生产β-LACTAM抗生素

    公开(公告)号：US20100009404A1

    公开(公告)日：2010-01-14

    申请号：US12444108

    申请日：2007-10-02

    申请人： Marcus Hans ,  Roelof Ary Lans Bovenberg ,  Paul Klaassen ,  Rémon Boer ,  Jan Metske Van Der Laan

    发明人： Marcus Hans ,  Roelof Ary Lans Bovenberg ,  Paul Klaassen ,  Rémon Boer ,  Jan Metske Van Der Laan

    IPC分类号： C12P37/00 ,  C12N9/00 ,  C12N15/52 ,  C12N1/21 ,  C12P35/00

    CPC分类号： C12N9/0004

    摘要： The present invention describes a process for the production of an N-α-amino-hydroxyphenylacetyl or an N-α-aminophenylacetyl β-lactam antibiotic comprising an IPNS-catalysed conversion of a precursor tripeptide hydroxyphenylglycyl-cysteinyl-valine (HpgCV) or phenylglycyl-cysteinyl-valine (PgCV), respectively, to the N-hydroxyphenylglycyl or the N-phenylglycyl β-lactam antibiotic, respectively. The tripeptide HpgCV or the tripeptide PgCV may further be prepared by contacting the amino acids hydroxyphenylglycine (Hpg) or phenylglycine (Pg), cystein (C) and valine (V) with a non-ribosomal peptide synthetase (NRPS) to effect formation of the tripeptide HpgCV or the tripeptide PgCV, the NRPS comprising a first module M1 specific for Hpg or Pg, a second module M2 specific for C and a third module M3 specific for V An IPNS is further provided having an improved activity in this conversion, as well as an NRPS catalysing the formation of the tripeptides. Also a host cell is provided capable of fermentatively producing β-lactam antibiotics with N-α-amino-hydroxyphenylacetyl or an N-α-aminophenylacetyl side chains.

    摘要翻译： 本发明描述了制备N-α-氨基 - 羟基苯基乙酰基或N-α-氨基苯基乙酰基β-内酰胺抗生素的方法，其包括前体三肽羟基苯基甘氨酰 - 半胱氨酰 - 缬氨酸（HpgCV）或苯基甘氨酰 - 半胱氨酰缬氨酸（PgCV）分别分别与N-羟基苯基甘氨酰或N-苯基甘氨酰β-内酰胺抗生素。 三肽HpgCV或三肽PgCV可以通过使氨基酸羟基苯基甘氨酸（Hpg）或苯基甘氨酸（Pg），半胱氨酸（C）和缬氨酸（V）与非核糖体肽合成酶（NRPS）接触来进一步制备，以形成 三肽HpgCV或三肽PgCV，NRPS包括特定于Hpg或Pg的第一模块M1，特定于C的第​​二模块M2和特定于V An IPNS的第三模块M3，还具有在该转换中具有改进的活性 作为催化三肽形成的NRPS。 还提供了能够用N-α-氨基 - 羟基苯基乙酰基或N-α-氨基苯乙酰侧链发酵生产β-内酰胺抗生素的宿主细胞。

    Production of β-Lactam antibiotics
    3.
    发明授权
    Production of β-Lactam antibiotics 有权
    生产＆bgr - 内酰胺抗生素

    公开(公告)号：US08293511B2

    公开(公告)日：2012-10-23

    申请号：US12444108

    申请日：2007-10-02

    申请人： Marcus Hans ,  Roelof Ary Lans Bovenberg ,  Paul Klaassen ,  Rémon Boer ,  Jan Metske Van Der Laan

    发明人： Marcus Hans ,  Roelof Ary Lans Bovenberg ,  Paul Klaassen ,  Rémon Boer ,  Jan Metske Van Der Laan

    IPC分类号： C12N9/00 ,  C12P37/00

    CPC分类号： C12N9/0004

    摘要： The present invention describes a process for the production of an N-α-amino-hydroxyphenylacetyl or an N-α-aminophenylacetyl β-lactam antibiotic comprising an IPNS-catalysed conversion of a precursor tripeptide hydroxyphenylglycyl-cysteinyl-valine (HpgCV) or phenylglycyl-cysteinyl-valine (PgCV), respectively, to the N-hydroxyphenylglycyl or the N-phenylglycyl β-lactam antibiotic, respectively. The tripeptide HpgCV or the tripeptide PgCV may further be prepared by contacting the amino acids hydroxyphenylglycine (Hpg) or phenylglycine (Pg), cystein (C) and valine (V) with a non-ribosomal peptide synthetase (NRPS) to effect formation of the tripeptide HpgCV or the tripeptide PgCV, the NRPS comprising a first module M1 specific for Hpg or Pg, a second module M2 specific for C and a third module M3 specific for V An IPNS is further provided having an improved activity in this conversion, as well as an NRPS catalysing the formation of the tripeptides. Also a host cell is provided capable of fermentatively producing β-lactam antibiotics with N-α-amino-hydroxyphenylacetyl or an N-α-aminophenylacetyl side chains.

    摘要翻译： 本发明描述了一种制备N-α-氨基 - 羟基苯基乙酰基或N-α-氨基苯基乙酰基 - 内酰胺抗生素的方法，其包括前体三肽羟基苯基甘氨酰 - 半胱氨酰 - 缬氨酸（HpgCV）或苯基甘氨酰 - 半胱氨酰缬氨酸（PgCV）分别转化为N-羟基苯基甘氨酰或N-苯基甘氨酰和β-内酰胺抗生素。 三肽HpgCV或三肽PgCV可以通过使氨基酸羟基苯基甘氨酸（Hpg）或苯基甘氨酸（Pg），半胱氨酸（C）和缬氨酸（V）与非核糖体肽合成酶（NRPS）接触来进一步制备，以形成 三肽HpgCV或三肽PgCV，NRPS包括特定于Hpg或Pg的第一模块M1，特定于C的第​​二模块M2和特定于V An IPNS的第三模块M3，还具有在该转换中具有改进的活性 作为催化三肽形成的NRPS。 还提供了能够用N-α-氨基 - 羟基苯基乙酰基或N-α-氨基苯乙酰侧链发酵生产β-内酰胺抗生素的宿主细胞。

    Pentose sugar fermenting cell
    5.
    发明授权
    Pentose sugar fermenting cell 有权
    戊糖发酵细胞

    公开(公告)号：US08399215B2

    公开(公告)日：2013-03-19

    申请号：US12867927

    申请日：2009-03-05

    申请人： Paul Klaassen ,  Jan Metske Van Der Laan ,  Bianca Elisabeth Maria Gielesen ,  Gijsberdina Pieternella Van Suylekom

    发明人： Paul Klaassen ,  Jan Metske Van Der Laan ,  Bianca Elisabeth Maria Gielesen ,  Gijsberdina Pieternella Van Suylekom

    IPC分类号： C12P1/00 ,  C12N1/00

    CPC分类号： C12N9/92 ,  C12P7/10 ,  Y02E50/16

    摘要： The invention relates to a cell which comprises a nucleotide sequence encoding a xylose isomerase, wherein the amino acid sequence of the xylose isomerase has at least about 70% sequence identity to the amino acid sequence set out in SEQ ID NO: 3 and wherein the nucleotide sequence is heterologous to the host. The cell of the invention may be used in a process for producing a fermentation product, such as ethanol. Such a process may comprise fermenting a medium containing a source of xylose with a cell of the invention such that the cell ferments xylose to the fermentation product.

    摘要翻译： 本发明涉及包含编码木糖异构酶的核苷酸序列的细胞，其中木糖异构酶的氨基酸序列与SEQ ID NO：3所示的氨基酸序列具有至少约70％的序列同一性，其中所述核苷酸 序列与宿主异源。 本发明的细胞可以用于生产发酵产物如乙醇的方法。 这样的方法可以包括用本发明的细胞发酵含有木糖来源的培养基，使得细胞向发酵产物发酵木糖。