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    Method for isolating nucleic acids
    1.
    发明授权
    Method for isolating nucleic acids 有权

    公开(公告)号：US09809612B2

    公开(公告)日：2017-11-07

    申请号：US12995348

    申请日：2009-05-12

    Applicant: Christoph Ritt ,  Martin Horlitz ,  Markus Sprenger-Haussels

    Inventor： Christoph Ritt ,  Martin Horlitz ,  Markus Sprenger-Haussels

    IPC: C07H1/08 ,  C12N15/10

    CPC classification number: C07H1/08 ,  C12N15/1006

    Abstract: The invention relates to a method and kits for isolating and/or purifying nucleic acids, in particular, short-chain nucleic acids, from a nucleic acid containing starting material, characterized by the following method steps: (a) bonding the nucleic acids to a nucleic acid bonding support material, wherein the starting material is brought into contact with the nucleic acid bonding support material in the presence of at least one chaotropic compound and preferably isopropanol, wherein the isopropanol is present in a concentration of ≧15% (v/v) and ≦35% (v/v), (b) optional elution of the bonded nucleic acids from the nucleic acid bonding support material. Said method is particularly suitable for the purification of foetal DNA from maternal blood.

    Explosive powder charge operated setting tool
    4.
    发明授权
    Explosive powder charge operated setting tool 有权
    爆炸粉充电操作设定工具

    公开(公告)号：US06126055A

    公开(公告)日：2000-10-03

    申请号：US495808

    申请日：2000-02-01

    Applicant: Gebhard Gantner ,  Markus Sprenger

    Inventor： Gebhard Gantner ,  Markus Sprenger

    IPC: B25C1/10 ,  B25C1/14 ,  B25D9/11

    CPC classification number: B25D9/11

    Abstract: An explosive powder charge-operated setting tool including a guide cylinder (2) displaceable relative to the tool housing (1), a drive piston (4) displaceably arranged in the inner bore (25) of the guide cylinder (2), a control pin (8) projecting from the guide cylinder (2) in a direction opposite to a setting direction, a spring-biased firing pin (16) cooperating with the control pin (8), a release pusher (19) for displacing the mating surface (26) of the firing pin (16) out of an axial projection surface of the control pin (8), a trigger (15) for actuating the release pusher (19), and a back-up pin (9) projecting into the inner bore (25) of the guide cylinder (2), with a smallest distance (A3) between the back-up pin (9) and a bottom (28) of the inner bore (25) facing in the setting direction corresponding to a length of the drive piston (4) between its end surface facing in the direction opposite to the setting direction and its stop surface (29) facing in the setting direction and cooperating with the back-up pin (9), and with a smallest distance (A1) between the back-up pin (9) and a free end surface of the control pin (8) plus a clearance (5) between the mating surface (26) of the firing pin (16) and the free end surface of the control pin (8) corresponding to a smallest distance (A2) between the back-up pin (9) and the mating surface (26) of the firing pin (16).

    Abstract translation: 一种爆炸性粉末充电操作固定工具，包括可相对于工具壳体（1）移位的引导筒（2），可移动地布置在引导筒（2）的内孔（25）中的驱动活塞（4） 销（8）沿着与设定方向相反的方向从所述引导筒（2）突出;弹簧偏压的击发销（16），与所述控制销（8）配合;释放推动器（19），用于使所述配合表面 （16）的突出部（26）离开控制销（8）的轴向投影表面，用于致动释放推动器（19）的触发器（15）和突出到所述控制销 所述导向缸（2）的内孔（25）在所述支撑销（9）和所述内孔（25）的底部（28）之间的最小距离（A3）处于面向所述设置方向 所述驱动活塞（4）在其与所述设定方向相反的方向的端面与其设置方向上的止挡面（29）之间的长度，以及 与支撑销（9）配合，并且在支撑销（9）和控制销（8）的自由端表面之间具有最小距离（A1）以及配合表面之间的间隙（5） （16）的控制销（26）和控制销（8）的自由端表面相对应于支撑销（9）和止动销（9）的配合表面（26）之间的最小距离（A2） （16）。

    Explosive powder charge operated setting tool
    5.
    发明授权
    Explosive powder charge operated setting tool 失效
    爆炸粉充电操作设定工具

    公开(公告)号：US5767434A

    公开(公告)日：1998-06-16

    申请号：US762174

    申请日：1996-12-09

    Applicant: Anton Hirtl ,  Markus Sprenger

    Inventor： Anton Hirtl ,  Markus Sprenger

    IPC: B25C1/10 ,  B25C1/14

    CPC classification number: B25C1/14

    Abstract: An explosive powder charge operated setting tool includes a housing (1), a piston guide (13) for a driving piston (9) located in the housing. The driving piston (9) can be propelled from an initial rearward position through a bore (18) in the piston guide (13) into a forward driving position by gases generated from an ignited cartridge charge (12) in a cartridge chamber (10) located rearwardly of the initial rearward position of the driving piston. A channel (18a, 18b) is located in the housing in flow communication with the cartridge chamber and communicating with the guide bore (8) in the piston guide (13). A check valve (5) is positioned in the channel (18a, 18b) and forms a storage space closed to the ambient atmosphere and made up of a section (18a) of the channel (18a, 18b) closer to the forward driving position of the driving piston and of the guide bore (8). The check valve (5) can be closed by the gas in the storage space.

    Abstract translation: 爆炸性粉末充电操作设定工具包括壳体（1），用于位于壳体中的驱动活塞（9）的活塞引导件（13）。 驱动活塞（9）可以从初始向后位置通过活塞导向件（13）中的孔（18）推进到通过在盒室（10）中点燃的料筒装料（12）产生的气体向前驱动位置， 位于驱动活塞的初始向后位置的后方。 通道（18a，18b）位于壳体中，与暗盒室流动连通，并与活塞引导件（13）中的导向孔（8）连通。 止回阀（5）定位在通道（18a，18b）中，并形成一个与环境大气相邻的储存空间，并由更靠近前述行驶位置的通道（18a，18b）的一部分（18a）构成 驱动活塞和导向孔（8）。 止回阀（5）可以被储存空间中的气体封闭。

    Isolation of nucleic acid from stool samples and other biological materials which are rich in inhibitors
    6.
    发明授权
    Isolation of nucleic acid from stool samples and other biological materials which are rich in inhibitors 有权

    公开(公告)号：US09890413B2

    公开(公告)日：2018-02-13

    申请号：US11338002

    申请日：2006-01-24

    Applicant: Markus Sprenger-Haussels

    Inventor： Markus Sprenger-Haussels

    IPC: C12Q1/68

    CPC classification number: C12Q1/6806 ,  C12Q2527/137 ,  C12Q2527/119

    Abstract: The invention related to a method for the stabilization, purification or/and isolation of nucleic acids from material samples, in particular, stool samples, which can contain impurities and inhibitors or interfering substances. The invention further relates to a reagent kit for carrying out this method. The basis of the invention is, in particular, a method for purification, stabilization or/and isolation of nucleic acids from material samples, whereby a buffer is added to the sample containing the nucleic acids, with a pH value of 2 to 7, a salt concentration of at least 100 mM, or/and a phenol neutralizing substance. According to the invention, pure nucleic acids which may be amplified can be obtained from faecal samples by a simple method, which are suitable for diagnostic proof of infection, in particular, bacterial or viral infection, or mutation, in particular, for tumor-specific DNA mutations.

    METHOD FOR ISOLATING SHORT-CHAIN NUCLEIC ACIDS
    7.
    发明申请
    METHOD FOR ISOLATING SHORT-CHAIN NUCLEIC ACIDS 有权
    用于分离短链核酸的方法

    公开(公告)号：US20110160446A1

    公开(公告)日：2011-06-30

    申请号：US12995341

    申请日：2009-05-12

    Applicant: Christoph Ritt ,  Martin Horlitz ,  Markus Sprenger-Haussels

    Inventor： Christoph Ritt ,  Martin Horlitz ,  Markus Sprenger-Haussels

    IPC: C07H1/06

    CPC classification number: C07H1/08 ,  C12N15/1006

    Abstract: The invention relates to a method and kits for isolating and/or purifying nucleic acids, in particular, short-chain nucleic acids, from a nucleic acid containing starting material, characterised by the following method steps: (a) bonding the nucleic acids to a nucleic acid bonding support material, wherein the starting material is brought into contact with the nucleic acid bonding support material in the presence of at least one chaotropic compound and preferably isopropanol, wherein the isopropanol is present in a concentration of ≧25% (v/v) and ≦35% (v/v), (b) optional elution of the bonded nucleic acids from the nucleic acid bonding support material. Said method is particularly suitable for the purification of foetal DNA from maternal blood.

    Abstract translation: 本发明涉及用于从含有起始物质的核酸分离和/或纯化核酸，特别是短链核酸的方法和试剂盒，其特征在于以下方法步骤：（a）将核酸结合至 核酸结合载体材料，其中原料在至少一种离液序列化合物和优选异丙醇的存在下与核酸键合载体材料接触，其中异丙醇以≥25％（v / v）的浓度存在 ）和＆lt; nlE; 35％（v / v），（b）从核酸粘合载体材料任选洗脱键合的核酸。 所述方法特别适用于从母体血液中纯化胎儿DNA。

    Nucleic acid isolation from stool samples and other inhibitor-rich biological materials
    8.
    发明授权
    Nucleic acid isolation from stool samples and other inhibitor-rich biological materials 有权
    从粪便样品和其他抑制剂丰富的生物材料中分离核酸

    公开(公告)号：US07005266B2

    公开(公告)日：2006-02-28

    申请号：US10182950

    申请日：2001-01-18

    Applicant: Markus Sprenger-Haussels

    Inventor： Markus Sprenger-Haussels

    IPC: C12Q1/68

    CPC classification number: C12Q1/6806 ,  C12Q2527/137 ,  C12Q2527/119

    Abstract: The invention relates to a method for the stabilization, purification or/and isolation of nucleic acids from material samples, in particular, stool samples, which can contain impurities and inhibitors or interfering substances. The invention further relates to a reagent kit for carrying out said method. The basis of the invention is, in particular, a method for purification, stabilization or/and isolation of nucleic acids from material samples, whereby a buffer is added to the sample containing the nucleic acids, with a pH value of 2 to 7, a salt concentration of at least 100 mM, or/and a phenol neutralizing substance. According to the invention, pure nucleic acids which may be amplified can be obtained from faecal samples by a simple method, which are suitable for diagnostic proof of infections, in particular, bacterial or viral infections, or mutations, in particular, for tumour-specific DNA mutations.

    Abstract translation: 本发明涉及从材料样品，特别是可以含有杂质和抑制剂或干扰物质的粪便样品中稳定，纯化或/和分离核酸的方法。 本发明还涉及用于执行所述方法的试剂盒。 本发明的基础尤其是用于从材料样品中纯化，稳定或/和分离核酸的方法，由此将缓冲液加入到含有核酸的样品中，pH值为2-7，a 盐浓度至少为100mM，或/和苯酚中和物质。 根据本发明，可以通过简单的方法从粪便样品获得可以扩增的纯核酸，其适用于感染的诊断性证据，特别是细菌或病毒感染或突变，特别是肿瘤特异性 DNA突变。

    Internal combustion powered tool for driving fastening elements
    9.
    发明授权
    Internal combustion powered tool for driving fastening elements 失效
    内燃机动力驱动元件

    公开(公告)号：US5213247A

    公开(公告)日：1993-05-25

    申请号：US775704

    申请日：1991-10-10

    Applicant: Hans Gschwend ,  Markus Sprenger

    Inventor： Hans Gschwend ,  Markus Sprenger

    IPC: B25C1/08 ,  B25D9/10 ,  F02B23/00 ,  F02B63/02 ,  F02B71/04 ,  F02B75/04 ,  F02M9/02

    CPC classification number: F02B75/04 ,  B25C1/08 ,  B25D9/10 ,  F02B71/04

    Abstract: A portable, internal combustion power operated working device, such as a setting tool for driving fastening elements into a receiving material, has a combustion chamber (9) for burning an air-fuel mixture. A piston (7, 7a), guided within a guide cylinder (8), is driven by the gas pressure generated by the combustion of the air-fuel mixture. The combustion chamber volume can be varied by at least one displaceable combustion chamber wall (11). To prepare the air-fuel mixture, a metering chamber (67) holds a predetermined quantity of gaseous fuel. Fuel from the metering chamber along with air is drawn into the combustion chamber by a negative pressure produced in the combustion chamber when the combustion chamber volume is increased by displacing the at least one combustion chamber wall (11).

    Abstract translation: 一种用于将紧固元件驱动到接收材料中的便携式内燃动力操作工作装置，例如用于将紧固元件驱动到接收材料中的固定工具，具有用于燃烧空气 - 燃料混合物的燃烧室（9）。 在引导筒（8）内引导的活塞（7,7a）由空气 - 燃料混合物的燃烧产生的气体压力驱动。 燃烧室容积可以由至少一个可移动的燃烧室壁（11）改变。 为了制备空气 - 燃料混合物，计量室（67）保持预定量的气体燃料。 当通过移动至少一个燃烧室壁（11）来增加燃烧室体积时，来自计量室的燃料与空气一起被燃烧室中产生的负压吸入燃烧室。

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