公开(公告)号：US20100326909A1

公开(公告)日：2010-12-30

申请号：US12464153

申请日：2009-05-12

申请人： Michael Vogeser

发明人： Michael Vogeser

IPC分类号： B01D15/42 ,  G01N33/00 ,  B03C1/00

CPC分类号： B03C1/015 ,  B01L2200/0647 ,  B03C1/288 ,  B03C2201/18 ,  G01N30/08 ,  G01N2030/085 ,  G01N2030/8813 ,  G01N2446/20

摘要： Described are a device and a method for the manipulation of a liquid sample material in which magnetic microparticles are suspended whereby the microparticles have a functionalized surface and an analyte is bound to the surface. The sample material is introduced into a device with a liquid system through an injection device (50) and in a first mobile phase the sample material is carried to an extractor (90). In a section (97) of the extractor (90) the microparticles are immobilized by means of a magnetic field of a controllable means (96) and separated from the remaining sample material. By switching over of a switching unit (110) a second mobile phase (75) is carried to the extractor (90) and the second mobile phase (75) detaches the adsorbed analyte from the surface of the microparticles. The second mobile phase (75) with the dissolved analyte(s) can be analyzed by way of chromatographic separation (130) and subsequent detection (140).

摘要翻译： 描述了用于操纵其中悬浮磁性微粒的液体样品材料的装置和方法，由此微粒具有官能化表面并且分析物结合到表面。 将样品材料通过注射装置（50）引入具有液体系统的装置中，并且在第一流动相中将样品材料运送到提取器（90）。 在提取器（90）的部分（97）中，微粒通过可控制装置（96）的磁场被固定，并与剩余的样品材料分离。 通过切换开关单元（110），将第二流动相（75）运送到提取器（90），并且第二流动相（75）将吸附的分析物从微粒表面分离。 可以通过色谱分离（130）和随后的检测（140）分析具有溶解的分析物的第二流动相（75）。

公开(公告)号：US20100291712A1

公开(公告)日：2010-11-18

申请号：US12328228

申请日：2008-12-04

申请人： Michael Vogeser

发明人： Michael Vogeser

IPC分类号： G01N33/553 ,  B03C1/30 ,  B01D15/08 ,  G01N1/34 ,  G01N21/00 ,  G01N27/26

CPC分类号： G01N30/08 ,  B03C1/015 ,  B03C1/288 ,  B03C2201/18 ,  G01N2030/085 ,  G01N2030/8813 ,  G01N2446/20

摘要： The invention concerns a device and a method for the manipulation of a liquid sample material in which magnetic microparticles are suspended whereby the microparticles have a functionalized surface and an analyte is bound to the surface. The sample material is introduced into a device with a liquid system through an injection device (50) and in a first mobile phase the sample material is carried to an extractor (90). In a first section (97a) of the extractor (90) the microparticles are immobilized by means of a magnetic field of a controllable device (96) and separated from the remaining sample material. By switching over of a switching unit (110) a second mobile phase (75) is carried to the extractor (90) and the second mobile phase (75) detaches the adsorbed analyte from the surface of the microparticles. In a second section (97b) of the extractor (90) the microparticles are immobilized and the second mobile phase (75) with the dissolved analyte(s) can be analyzed by way of chromatographic separation (130) and subsequent detection (140).

摘要翻译： 本发明涉及用于操纵其中悬浮磁性微粒的液体样品材料的装置和方法，由此微粒具有官能化表面并且分析物结合到表面。 将样品材料通过注射装置（50）引入具有液体系统的装置中，并且在第一流动相中将样品材料运送到提取器（90）。 在提取器（90）的第一部分（97a）中，微粒通过可控设备（96）的磁场被固定，并与剩余的样品材料分离。 通过切换开关单元（110），将第二流动相（75）运送到提取器（90），并且第二流动相（75）将吸附的分析物从微粒表面分离。 在提取器（90）的第二部分（97b）中，微粒被固定，并且可以通过色谱分离（130）和随后的检测（140）分析具有溶解的分析物的第二流动相（75）。

公开(公告)号：US08829429B2

公开(公告)日：2014-09-09

申请号：US13379180

申请日：2010-06-18

申请人： Roland Geyer ,  Werner Halg ,  Michael Vogeser

发明人： Roland Geyer ,  Werner Halg ,  Michael Vogeser

IPC分类号： H01J49/00 ,  G01N30/02

CPC分类号： H01J49/0009 ,  G01N30/02 ,  G01N30/7233 ,  G01N30/8665 ,  G01N2030/045 ,  H01J49/0036

摘要： Test method checks and compensates ion yield variances in a mass spectrometer and includes feeding an eluate of a chromatographic separation to the spectrometer, continuously ad-mixing a separate target analyte solution at known concentration and constant flow rate to the eluate, injecting the mixture into the spectrometer and generating a detector signal for the mixture. A spectrogram is captured which includes an integration line and a mass-spectrographic peak of the target analyte, the integration line being a lasting background signal underlaid to the mass-spectrometric analysis of the eluate, then evaluating the spectrogram by capturing an integrated mass-spectrographic peak area above the integration line and an area which is found under the integrated peak area of the target analyte by perpendicular drop lines from the peak integration line and forming a mathematical relationship from the determined mass-spectrographic areas.

摘要翻译： 测试方法检查和补偿质谱仪中的离子产量变化，并且包括将色谱分离的洗脱液加入到光谱仪中，将已知浓度和恒定流速的单独的目标分析物溶液连续混合到洗脱液中，将混合物注入 并产生混合物的检测器信号。 捕获的光谱图包括目标分析物的积分线和质谱图峰，该整合线是一个持续的背景信号，其被嵌入洗脱液的质谱分析中，然后通过捕获整合的质谱仪来评估光谱图 峰面积高于目标分析物的集成峰面积的区域，通过来自峰积分线的垂直投影线，并从确定的质谱图形成数学关系。

公开(公告)号：US08337705B2

公开(公告)日：2012-12-25

申请号：US12464153

申请日：2009-05-12

申请人： Michael Vogeser

发明人： Michael Vogeser

IPC分类号： B03C1/02 ,  G01N1/34 ,  G01N21/00

CPC分类号： B03C1/015 ,  B01L2200/0647 ,  B03C1/288 ,  B03C2201/18 ,  G01N30/08 ,  G01N2030/085 ,  G01N2030/8813 ,  G01N2446/20

摘要： Described are a device and a method for the manipulation of a liquid sample material in which magnetic microparticles are suspended whereby the microparticles have a functionalized surface and an analyte is bound to the surface. The sample material is introduced into a device with a liquid system through an injection device (50) and in a first mobile phase the sample material is carried to an extractor (90). In a section (97) of the extractor (90) the microparticles are immobilized by means of a magnetic field of a controllable means (96) and separated from the remaining sample material. By switching over of a switching unit (110) a second mobile phase (75) is carried to the extractor (90) and the second mobile phase (75) detaches the adsorbed analyte from the surface of the microparticles. The second mobile phase (75) with the dissolved analyte(s) can be analyzed by way of chromatographic separation (130) and subsequent detection (140).

摘要翻译： 描述了用于操纵其中悬浮磁性微粒的液体样品材料的装置和方法，由此微粒具有官能化表面并且分析物结合到表面。 将样品材料通过注射装置（50）引入具有液体系统的装置中，并且在第一流动相中将样品材料运送到提取器（90）。 在提取器（90）的部分（97）中，微粒通过可控制装置（96）的磁场被固定，并与剩余的样品材料分离。 通过切换开关单元（110），将第二流动相（75）运送到提取器（90），并且第二流动相（75）将吸附的分析物从微粒表面分离。 可以通过色谱分离（130）和随后的检测（140）分析具有溶解的分析物的第二流动相（75）。

公开(公告)号：US08337704B2

公开(公告)日：2012-12-25

申请号：US12328228

申请日：2008-12-04

申请人： Michael Vogeser

发明人： Michael Vogeser

IPC分类号： B03C1/02 ,  G01N1/34 ,  G01N21/00

CPC分类号： G01N30/08 ,  B03C1/015 ,  B03C1/288 ,  B03C2201/18 ,  G01N2030/085 ,  G01N2030/8813 ,  G01N2446/20

摘要： The invention concerns a device and a method for the manipulation of a liquid sample material in which magnetic microparticles are suspended whereby the microparticles have a functionalized surface and an analyte is bound to the surface. The sample material is introduced into a device with a liquid system through an injection device (50) and in a first mobile phase the sample material is carried to an extractor (90). In a first section (97a) of the extractor (90) the microparticles are immobilized by means of a magnetic field of a controllable device (96) and separated from the remaining sample material. By switching over of a switching unit (110) a second mobile phase (75) is carried to the extractor (90) and the second mobile phase (75) detaches the adsorbed analyte from the surface of the microparticles. In a second section (97b) of the extractor (90) the microparticles are immobilized and the second mobile phase (75) with the dissolved analyte(s) can be analyzed by way of chromatographic separation (130) and subsequent detection (140).

摘要翻译： 本发明涉及用于操纵其中悬浮磁性微粒的液体样品材料的装置和方法，由此微粒具有官能化表面并且分析物结合到表面。 将样品材料通过注射装置（50）引入具有液体系统的装置中，并且在第一流动相中将样品材料运送到提取器（90）。 在提取器（90）的第一部分（97a）中，微粒通过可控设备（96）的磁场被固定，并与剩余的样品材料分离。 通过切换开关单元（110），将第二流动相（75）运送到提取器（90），并且第二流动相（75）将吸附的分析物从微粒表面分离。 在提取器（90）的第二部分（97b）中，微粒被固定，并且可以通过色谱分离（130）和随后的检测（140）分析具有溶解的分析物的第二流动相（75）。

公开(公告)号：US20120187284A1

公开(公告)日：2012-07-26

申请号：US13379180

申请日：2010-06-18

申请人： Roland Geyer ,  Werner Halg ,  Michael Vogeser

发明人： Roland Geyer ,  Werner Halg ,  Michael Vogeser

IPC分类号： G01D18/00 ,  G01N30/00

CPC分类号： H01J49/0009 ,  G01N30/02 ,  G01N30/7233 ,  G01N30/8665 ,  G01N2030/045 ,  H01J49/0036

摘要： Test method checks and compensates ion yield variances in a mass spectrometer and includes feeding an eluate of a chromatographic separation to the spectrometer, continuously ad-mixing a separate target analyte solution at known concentration and constant flow rate to the eluate, injecting the mixture into the spectrometer and generating a detector signal for the mixture. A spectrogram is captured which includes an integration line and a mass-spectrographic peak of the target analyte, the integration line being a lasting background signal underlaid to the mass-spectrometric analysis of the eluate, then evaluating the spectrogram by capturing an integrated mass-spectrographic peak area above the integration line and an area which is found under the integrated peak area of the target analyte by perpendicular drop lines from the peak integration line and forming a mathematical relationship from the determined mass-spectrographic areas.

摘要翻译： 测试方法检查和补偿质谱仪中的离子产量变化，并且包括将色谱分离的洗脱液加入到光谱仪中，将已知浓度和恒定流速的单独的目标分析物溶液连续混合到洗脱液中，将混合物注入 并产生混合物的检测器信号。 捕获的光谱图包括目标分析物的积分线和质谱图峰，该整合线是一个持续的背景信号，其被嵌入洗脱液的质谱分析中，然后通过捕获整合的质谱仪来评估光谱图 峰面积高于目标分析物的集成峰面积的区域，通过来自峰积分线的垂直投影线，并从确定的质谱图形成数学关系。

公开(公告)号：US07815803B2

公开(公告)日：2010-10-19

申请号：US12133545

申请日：2008-06-05

申请人： Uwe Kobold ,  Albert Geiger ,  Rupert Herrmann ,  Michael Vogeser

发明人： Uwe Kobold ,  Albert Geiger ,  Rupert Herrmann ,  Michael Vogeser

IPC分类号： B01D15/08 ,  B01D59/44 ,  H01J49/00

CPC分类号： G01N33/6848 ,  B01D15/325 ,  B01J20/28009 ,  B01J20/287 ,  B01J20/3246 ,  B01J20/3276 ,  G01N33/54326 ,  G01N33/6842

摘要： The present invention provides a novel procedure of preparing samples for analysis by way of mass spectrometry, preferably LC-MS/MS. Accordingly, functionalized magnetic particles with a hydrophobic surface were used for extracting low molecular weight compounds from complex liquid biological samples such as plasma, serum, whole blood or hemolyzed blood. The method of the invention includes (a) contacting the sample with an amount of functionalized magnetic particles with a hydrophobic surface, (b) incubating the sample and the particles, thereby adsorbing the compound to the hydrophobic surface, (c) separating the particles by applying a magnetic field and removing the liquid, (d) optionally washing the particles, (e) eluting the compound from the particles.

摘要翻译： 本发明提供了通过质谱法，优选LC-MS / MS制备用于分析的样品的新方法。 因此，使用具有疏水性表面的官能化磁性颗粒从复杂的液体生物样品如血浆，血清，全血或溶血中提取低分子量化合物。 本发明的方法包括（a）使样品与一定量的具有疏水性表面的官能化磁性颗粒接触，（b）将样品和颗粒温育，从而将化合物吸附到疏水表面，（c）将颗粒分离 施加磁场并除去液体，（d）任选地洗涤颗粒，（e）从颗粒中洗脱化合物。

公开(公告)号：US20090090855A1

公开(公告)日：2009-04-09

申请号：US12133545

申请日：2008-06-05

申请人： Uwe Kobold ,  Albert Geiger ,  Rupert Herrmann ,  Michael Vogeser

发明人： Uwe Kobold ,  Albert Geiger ,  Rupert Herrmann ,  Michael Vogeser

IPC分类号： H01J49/00 ,  B01D15/26

CPC分类号： G01N33/6848 ,  B01D15/325 ,  B01J20/28009 ,  B01J20/287 ,  B01J20/3246 ,  B01J20/3276 ,  G01N33/54326 ,  G01N33/6842

摘要： The present invention provides a novel procedure of preparing samples for analysis by way of mass spectrometry, preferably LC-MS/MS. Accordingly, functionalized magnetic particles with a hydrophobic surface were used for extracting low molecular weight compounds from complex liquid biological samples such as plasma, serum, whole blood or hemolyzed blood. The method of the invention includes (a) contacting the sample with an amount of functionalized magnetic particles with a hydrophobic surface, (b) incubating the sample and the particles, thereby adsorbing the compound to the hydrophobic surface, (c) separating the particles by applying a magnetic field and removing the liquid, (d) optionally washing the particles, (e) eluting the compound from the particles.

摘要翻译： 本发明提供了通过质谱法，优选LC-MS / MS制备用于分析的样品的新方法。 因此，使用具有疏水性表面的官能化磁性颗粒从复杂的液体生物样品如血浆，血清，全血或溶血中提取低分子量化合物。 本发明的方法包括（a）使样品与一定量的具有疏水性表面的官能化磁性颗粒接触，（b）将样品和颗粒温育，从而将化合物吸附到疏水表面，（c）将颗粒分离 施加磁场并除去液体，（d）任选地洗涤颗粒，（e）从颗粒中洗脱化合物。