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    REAGENT FLUID DISPENSING DEVICE, AND METHOD OF DISPENSING A REAGENT FLUID
    1.
    发明申请
    REAGENT FLUID DISPENSING DEVICE, AND METHOD OF DISPENSING A REAGENT FLUID 有权
    试剂流体分配装置和分配试剂流体的方法

    公开(公告)号：US20130136671A1

    公开(公告)日：2013-05-30

    申请号：US13696063

    申请日：2011-05-04

    申请人： Mo-Huang Li ,  Jackie Y. Yang ,  Guolin Xu ,  Yoke San Daniel Lee ,  Emril Mohamed Ali ,  Tseng-Ming Hsieh

    发明人： Mo-Huang Li ,  Jackie Y. Yang ,  Guolin Xu ,  Yoke San Daniel Lee ,  Emril Mohamed Ali ,  Tseng-Ming Hsieh

    IPC分类号： B01L3/00 ,  B01L3/02

    CPC分类号： B01L3/52 ,  F04F1/18

    摘要： According to various embodiments, a reagent fluid dispensing device may be provided. The reagent fluid dispensing device may include a chamber for receiving a reagent fluid, the chamber having a first opening and a second opening; a first fluid conduit connected to the first opening of the chamber; a reservoir connected to the first fluid conduit, the reservoir having a first opening, wherein the first opening of the reservoir is connected to the first fluid conduit to form a passive valve, wherein the reservoir is dimensionalized for storing a predetermined volume of the reagent fluid; and a pneumatic conduit connected to the second opening of the chamber, wherein selective application of pneumatic pressure to the chamber through the pneumatic conduit transfers the reagent fluid from the reservoir to the chamber through the first fluid conduit. According to various embodiments, a microfluidic device including the reagent fluid dispensing device, and a method of dispensing a reagent fluid may be provided.

    摘要翻译： 根据各种实施例，可以提供试剂流体分配装置。 试剂流体分配装置可以包括用于接收试剂流体的腔室，该腔室具有第一开口和第二开口; 连接到所述室的第一开口的第一流体导管; 连接到第一流体管道的储存器，储存器具有第一开口，其中储存器的第一开口连接到第一流体导管以形成被动阀，其中储存器被尺寸化以存储预定体积的试剂流体 ; 以及连接到所述腔室的第二开口的气动导管，其中通过所述气动导管选择性地将气压施加到所述腔室通过所述第一流体导管将所述试剂流体从所述储存器传送到所述腔室。 根据各种实施例，可以提供包括试剂流体分配装置的微流体装置和分配试剂流体的方法。

    Image locking system for DNA micro-array synthesis
    3.
    发明授权
    Image locking system for DNA micro-array synthesis 有权
    用于DNA微阵列合成的图像锁定系统

    公开(公告)号：US07072500B2

    公开(公告)日：2006-07-04

    申请号：US10841847

    申请日：2004-05-07

    申请人： Francesco Cerrina ,  Mo-Huang Li ,  Chang-Han Kim

    发明人： Francesco Cerrina ,  Mo-Huang Li ,  Chang-Han Kim

    IPC分类号： G06K9/00

    CPC分类号： G02B26/0833 ,  B01J19/0046 ,  B01J2219/00439 ,  B01J2219/0054 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00693 ,  B01J2219/00722 ,  G02B7/1827 ,  G02B17/008 ,  G02B17/0615 ,  G02B19/0023 ,  G02B19/0047 ,  G02B19/0095

    摘要： An image locking system for DNA micro-array synthesis provides a feedback system to stabilize or lock the image with respect to an image capture device, such as a camera and/or microscope. The image locking system includes the use of detection or reference marks. When a shift in image position is detected, a correction signal is sent to one of two mirrors, moving the image to correct for the shift in image position. The system comprises a first light beam directed towards a micromirror device that forms an alignment pattern on a reaction cell and a second light beam directed towards the micromirror device that forms a micro-array image on an active surface of the reaction cell. A camera captures the alignment pattern and an alignment mark. A computer calculates a correction signal to realign the alignment pattern with the alignment mark when movement is detected.

    摘要翻译： 用于DNA微阵列合成的图像锁定系统提供反馈系统以相对于图像捕获装置（诸如照相机和/或显微镜）稳定或锁定图像。 图像锁定系统包括使用检测或参考标记。 当检测到图像位置的偏移时，将校正信号发送到两个反射镜中的一个，移动图像以校正图像位置的偏移。 该系统包括朝向微反射镜装置的第一光束，该微镜装置在反应单元上形成对准图案，以及朝向微反射镜装置的第二光束，其在反应池的活性表面上形成微阵列图像。 相机拍摄对准图案和对准标记。 当检测到移动时，计算机计算校正信号以使对准图案与对准标记重新对准。

    Image Locking System for Dna Micro-Array Synthesis
    5.
    发明申请
    Image Locking System for Dna Micro-Array Synthesis 审中-公开
    Dna微阵列合成图像锁定系统

    公开(公告)号：US20080266562A1

    公开(公告)日：2008-10-30

    申请号：US11568762

    申请日：2005-03-25

    申请人： Francesco Cerrina ,  Mo-Huang Li ,  Chang-Han Kim

    发明人： Francesco Cerrina ,  Mo-Huang Li ,  Chang-Han Kim

    IPC分类号： G01B11/00

    CPC分类号： G02B26/0833 ,  B01J19/0046 ,  B01J2219/00439 ,  B01J2219/0054 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00693 ,  B01J2219/00722 ,  G02B7/1827 ,  G02B17/008 ,  G02B17/0615 ,  G02B19/0023 ,  G02B19/0047 ,  G02B19/0095

    摘要： An image locking system for DNA micro-array synthesis provides a feedback system to stabilize or lock the image with respect to an image capture device, such as a camera and/or microscope. The image locking system includes the use of detection or reference marks. When a shift in image position is detected, a correction signal is sent to one of two mirrors, moving the image to correct for the shift in image position. The system comprises a first light beam directed towards a micromirror device that forms an alignment pattern on a reaction cell and a second light beam directed towards the micromirror device that forms a micro-array image on an active surface of the reaction cell. A camera captures the alignment pattern and an alignment mark. A computer calculates a correction signal to realign the alignment pattern with the alignment mark when movement is detected.

    摘要翻译： 用于DNA微阵列合成的图像锁定系统提供反馈系统以相对于图像捕获装置（诸如照相机和/或显微镜）稳定或锁定图像。 图像锁定系统包括使用检测或参考标记。 当检测到图像位置的偏移时，将校正信号发送到两个反射镜中的一个，移动图像以校正图像位置的偏移。 该系统包括朝向微反射镜装置的第一光束，该微镜装置在反应单元上形成对准图案，以及朝向微反射镜装置的第二光束，其在反应池的活性表面上形成微阵列图像。 相机拍摄对准图案和对准标记。 当检测到移动时，计算机计算校正信号以使对准图案与对准标记重新对准。

    GENE SYNTHESIS METHOD
    7.
    发明申请
    GENE SYNTHESIS METHOD 审中-公开
    基因合成方法

    公开(公告)号：US20120178129A1

    公开(公告)日：2012-07-12

    申请号：US13320255

    申请日：2009-05-11

    申请人： Mo Huang Li ,  Jackie Y. Yang ,  Chye Cheong Wai ,  Marcus Bode

    发明人： Mo Huang Li ,  Jackie Y. Yang ,  Chye Cheong Wai ,  Marcus Bode

    IPC分类号： C12P19/34 ,  C07H21/04

    CPC分类号： C12Q1/686 ,  C12N15/1096 ,  C12Q1/6811 ,  C12Q2527/107 ,  C12Q2525/204

    摘要： The present invention relates to polymerase chain reaction (PCR)-based methods for the one-step synthesis of nucleic acid molecules, wherein the amplification primers used in said methods are designed such that they have two distinct melting temperatures in order to minimize the competition between polymerase cycling assembly (PCA) and polymerase chain reaction (PCR) amplification in the one-step nucleic acid synthesis and to maximize the emerging full-length amplification, as well as kits for use in such methods.

    摘要翻译： 本发明涉及用于一步合成核酸分子的基于聚合酶链反应（PCR）的方法，其中所述方法中使用的扩增引物被设计成使得它们具有两个不同的熔融温度，以便最小化 聚合酶循环装置（PCA）和聚合酶链反应（PCR）在一步核酸合成中的扩增，并使新出现的全长扩增最大化，以及用于这些方法的试剂盒。

    Method and system for the generation of large double stranded DNA fragments
    9.
    发明申请
    Method and system for the generation of large double stranded DNA fragments 审中-公开
    用于产生大双链DNA片段的方法和系统

    公开(公告)号：US20070196834A1

    公开(公告)日：2007-08-23

    申请号：US11506150

    申请日：2006-08-17

    申请人： Francesco Cerrina ,  James Kaysen ,  Mo-Huang Li ,  Larry Chu ,  Peter Belshaw ,  Michael Sussman ,  Kathryn Richmond

    发明人： Francesco Cerrina ,  James Kaysen ,  Mo-Huang Li ,  Larry Chu ,  Peter Belshaw ,  Michael Sussman ,  Kathryn Richmond

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04

    CPC分类号： C12Q1/6813 ,  C12P19/34

    摘要： Synthesis of long chain molecules such as DNA is carried out rapidly and efficiently to produce relatively large quantities of the desired product. The synthesis of an entire gene or multiple genes formed of many hundreds or thousands of base pairs can be accomplished rapidly and, if desired, in a fully automated process requiring minimal operator intervention, and in a matter of hours, a day or a few days rather than many days or weeks. Production of a desired gene or set of genes having a specified base pair sequence is initiated by analyzing the specified target sequence and determining an optimal set of subsequences of base pairs that can be assembled to form the desired final target sequence. The set of oligonucleotides are then synthesized utilizing automated oligonucleotide synthesis techniques. The synthesized oligonucleotides are subsequently selectively released from the substrate and used in a sequential assembly process.

    摘要翻译： 长链分子（如DNA）的合成进行迅速且有效地产生相对大量的所需产物。 由数百或数千个碱基对形成的整个基因或多个基因的合成可以快速完成，并且如果需要，可以在需要最小操作者干预的完全自动化过程中完成，并且在几小时，一天或几天内 而不是很多天或几周。 通过分析指定的靶序列并确定可以组装以形成期望的最终目标序列的碱基对的最佳一组子序列来开始产生具有特定碱基对序列的所需基因或一组基因。 然后使用自动寡核苷酸合成技术合成寡核苷酸组。 合成的寡核苷酸随后从衬底中选择性释放并用于顺序组装过程。

    Micromachined scanning thermal probe method and apparatus
    10.
    发明授权
    Micromachined scanning thermal probe method and apparatus 失效
    微加工扫描热探针法和装置

    公开(公告)号：US06692145B2

    公开(公告)日：2004-02-17

    申请号：US10001919

    申请日：2001-10-31

    申请人： Yogesh B. Gianchandani ,  Mo-Huang Li ,  Julius Wu

    发明人： Yogesh B. Gianchandani ,  Mo-Huang Li ,  Julius Wu

    IPC分类号： G01K724

    CPC分类号： G01K7/028

    摘要： A micromachined thermal probe has a substrate with a surface and an edge, and at least one flexible probe body formed on the substrate that includes a cantilever beam section that extends from a proximal end outwardly to a distal end. A pair of conductors in the probe body extend to a junction at the distal end at which is formed a probe tip. Current passed through the conductors to the junction heats the probe tip, with changes in the effective probe resistance occurring as the probe tip is scanned over a sample with different thermal conductivities at different positions. A second flexible probe body may be mounted to the substrate and constructed similarly to the first probe body to act as a reference probe to allow compensation of the first probe. The probe body may be formed of layers of flexible polymer joined together over pairs of conductors, which is bent back onto itself and secured together at a proximal end of the cantilever beam.

    摘要翻译： 微加工热探针具有表面和边缘的基底，以及形成在基底上的至少一个柔性探针体，其包括从近端向外延伸到远端的悬臂梁部分。 探针主体中的一对导体延伸到远端处的一个连接处，在该连接处形成探针尖端。 通过导线穿过接头的电流会加热探针尖端，当探头尖端扫描不同位置不同导热系数的样品时，会发生有效探头电阻的变化。 第二柔性探针体可以安装到基底并且类似于第一探针体构造以用作参考探针以允许补偿第一探针。 探针主体可以由柔性聚合物层形成，这些层由成对的导体连接在一起，该导体被弯曲回自身并且在悬臂梁的近端处固定在一起。