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    Methods of detecting target nucleic acids
    3.
    发明授权
    Methods of detecting target nucleic acids 有权
    检测靶核酸的方法

    公开(公告)号：US08637247B2

    公开(公告)日：2014-01-28

    申请号：US13590794

    申请日：2012-08-21

    Applicant: Muhammad Sharaf ,  Timothy Woudenberg ,  Khairuzzaman Bashar Mullah

    Inventor： Muhammad Sharaf ,  Timothy Woudenberg ,  Khairuzzaman Bashar Mullah

    IPC: C12Q1/68

    CPC classification number: C12Q1/6825 ,  C12Q1/6816 ,  C12Q1/6823 ,  C12Q1/6827 ,  C12Q1/6832 ,  C12Q1/6876 ,  G01N27/44726 ,  G01N27/44791 ,  C12Q2565/631

    Abstract: The present disclosure relates to methods of identifying target nucleic acids by using coded molecules and its analysis by translocation through a nanopore. Generally, coded molecules are subject to a target polynucleotide dependent modification. The modified coded molecule is detected by isolating the modified coded molecules from the unmodified coded molecules prior to analysis through the nanopore or by detecting a change in the signal pattern of the coded molecule when analyzed through the nanopore.

    Abstract translation: 本公开内容涉及通过使用编码分子鉴定靶核酸的方法及其通过纳米孔易位的分析。 通常，经编码的分子经受目标多核苷酸依赖性修饰。 通过在通过纳米孔分析之前将经修饰的编码分子与未修饰的编码分子分离，或者当通过纳米孔分析时，通过检测编码分子的信号图谱的变化来检测修饰的编码分子。

    Spectral calibration of fluorescent polynucleotide separation apparatus
    4.
    发明申请
    Spectral calibration of fluorescent polynucleotide separation apparatus 审中-公开

    公开(公告)号：US20060102479A1

    公开(公告)日：2006-05-18

    申请号：US11323620

    申请日：2005-12-30

    Applicant: Muhammad Sharaf ,  Maria Roque-Biewer

    Inventor： Muhammad Sharaf ,  Maria Roque-Biewer

    IPC: B01D57/02

    CPC classification number: G01N27/44726 ,  Y10T436/10

    Abstract: The invention relates to methods, compositions, and systems for calibrating a fluorescent polynucleotide separation apparatus. One aspect of the invention is multiple color calibration standards and their use. A multiple color calibration standard is a mixture of at least two polynucleotides of different length, wherein each of the polynucleotides is labeled with a spectrally distinct fluorescent dye. Another aspect of the invention is to produce total emission temporal profiles of multiple color calibration standards for use in calibrating fluorescent polynucleotide separation apparatus. The peaks corresponding to the fluorescently labeled polynucleotides in the total emission temporal profile may be detected using a peak detector that is driven by changes in the slopes of the total emission temporal profile. Calibration of fluorescent polynucleotide separation apparatus, with various embodiments of the methods of the invention, includes the step of identification of the labeled polynucleotides of the multiple color calibration standards. The process of spectral calibration of a fluorescent polynucleotide separation apparatus using a multiple color calibration standard may include the step of the estimating (extracting) of the dyes' reference spectra, using information from the peak detection process performed on the total emission temporal profile. Other aspects of the invention include systems for separating and detecting fluorescently labeled polynucleotides, wherein the system is designed for spectral calibration in accordance with the subject calibration methods employing multiple color calibration standards. Another aspect of the invention is methods and compositions for detecting the flow of electrical current through a separation channel of a fluorescent polynucleotide separation apparatus. These methods and compositions employ monitoring dyes. Monitoring dyes are fluorescent dyes that are spectrally distinct from the dye on the polynucleotide intended to convey genetic information, e.g., fluorescent polynucleotide sequencing reaction products.

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