Plasmid originated from bifidobacterium, recombinant expression vector using the plasmid and transformation method
    2.
    发明授权
    Plasmid originated from bifidobacterium, recombinant expression vector using the plasmid and transformation method 失效
    质粒源自双歧杆菌,重组表达载体使用质粒和转化方法

    公开(公告)号:US06887702B2

    公开(公告)日:2005-05-03

    申请号:US10258440

    申请日:2001-03-30

    CPC分类号: C12N15/70 C12N15/746

    摘要: The present invention relates to a new plasmid originated from (Bifidobacterium) a recombinant expression vector and transformation method using the same. More particularly, the present invention relates to a plasmid pMG1 having nucleotide sequence represented by SEQ.ID.NO.1; (Bifidobacterium longum) MG1 including the plasmid pMG1; and a shuttle vector which can be replicated in both (Bifidobacterium) and (E. coli), and comprises (Mob) gene having nucleotide sequence represented by SEQ.ID.NO.2. (Rep) gene having nucleotide sequence represented by SEQ.ID.NO.3 and a selection marker. The shuttle vector and the promoter of the present invention can be used for expressing target gene without additional purification process. The protein expressed from the target gene in (Bifidobacterium) can be added to food, therefore, the protein can be used for preparing food additives or oral vaccine, Furthermore, the potential and the possibilty of probiotics using (Bifidobacterium) can promoted through the development of the shuttle vector.

    摘要翻译: 本发明涉及来自(双歧杆菌)的重组表达载体的新质粒和使用其的转化方法。 更具体地,本发明涉及具有由SEQ.ID.NO.1表示的核苷酸序列的质粒pMG1; (双歧杆菌)MG1,包含质粒pMG1; 和可以在(双歧杆菌)和(大肠杆菌)中复制的穿梭载体,并且包含具有由SEQ.ID.NO.2表示的核苷酸序列的(Mob)基因。 (Rep)基因,其具有由SEQ.ID.NO.3表示的核苷酸序列和选择标记。 本发明的穿梭载体和启动子可用于表达靶基因而无需额外的纯化过程。 从双歧杆菌中的目标基因表达的蛋白质可以添加到食品中,因此蛋白质可用于制备食品添加剂或口服疫苗。此外,使用(双歧杆菌)的益生菌的潜力和可能性可以通过开发来促进 的穿梭载体。