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11 条记录 (耗时 0.018 秒)

    Methods, Agents, and Compound Screening Assays for Inducing Differentiation of Undifferentiated Mammalian Cells into Osteoblasts
    1.
    发明申请
    Methods, Agents, and Compound Screening Assays for Inducing Differentiation of Undifferentiated Mammalian Cells into Osteoblasts 失效
    用于诱导未分化哺乳动物细胞分化成成骨细胞的方法，试剂和化合物筛选试验

    公开(公告)号：US20070184026A1

    公开(公告)日：2007-08-09

    申请号：US11551744

    申请日：2006-10-23

    申请人： Luc Van Rompaey ,  Peter Tomme ,  Robin Brown

    发明人： Luc Van Rompaey ,  Peter Tomme ,  Robin Brown

    IPC分类号： A61K48/00 ,  C40B30/06 ,  C40B40/08 ,  C40B40/10 ,  C12N15/86

    CPC分类号： G01N33/566 ,  C12N15/111 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/53 ,  C12N2320/11 ,  C12N2320/12 ,  G01N33/5026 ,  G01N2333/51 ,  G01N2500/04 ,  G01N2800/108

    摘要： The present invention relates to methods, agents and compound screening assays for inducing differentiation of undifferentiated mammalian cells into osteoblasts. The invention thus provides a method, comprising contacting a compound with a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID No: 194-309; and measuring a compound-polypeptide property related to the differentiation of said cells. The invention further relates to a bone formation enhancing pharmaceutical composition, and the use thereof in treating and/or preventing a disease involving a systemic or local decrease in mean bone density in a subject. Furthermore, the invention relates to a method for the in vitro production of bone tissue.

    摘要翻译： 本发明涉及用于诱导未分化的哺乳动物细胞分化成成骨细胞的方法，试剂和化合物筛选试验。 因此，本发明提供了一种方法，包括使化合物与包含选自SEQ ID No：194-309的氨基酸序列的多肽接触; 并测量与所述细胞的分化相关的化合物 - 多肽性质。 本发明进一步涉及增强骨形成的药物组合物，以及其在治疗和/或预防受试者的全身或局部平均骨密度降低的疾病中的用途。 此外，本发明涉及体外生产骨组织的方法。

    Purification of a polypeptide compound having a polysaccharide binding domain by affinity phase separation
    4.
    发明授权
    Purification of a polypeptide compound having a polysaccharide binding domain by affinity phase separation 失效
    通过亲和相分离纯化具有多糖结合结构域的多肽化合物

    公开(公告)号：US06174700B1

    公开(公告)日：2001-01-16

    申请号：US08505860

    申请日：1995-07-24

    申请人： Charles A. Haynes ,  Peter Tomme ,  Douglas G. Kilburn

    发明人： Charles A. Haynes ,  Peter Tomme ,  Douglas G. Kilburn

    IPC分类号： C12P2106

    CPC分类号： A61K39/39 ,  C07K1/14 ,  C07K14/55 ,  C07K2319/00 ,  C08B15/00 ,  C08B37/003 ,  C08H8/00 ,  C12N9/16 ,  C12N9/2437 ,  C12N9/2445 ,  C12N11/12 ,  C12P19/02 ,  C12Q1/56 ,  C12Y302/01004 ,  C12Y302/01021 ,  D21H17/005 ,  G01N33/531 ,  G01N2333/924 ,  G01N2400/26 ,  Y10S435/803 ,  Y10S435/815 ,  Y10S530/813 ,  Y10S530/814

    摘要： A compound having a polysaccharide binding domain such as contained by a cellulose and essentially lacking in polysaccharidase activity is purified from other ingredients in a mixture using an affinity partition system. A mixture containing the compound is contacted with a system containing as a first phase an aqueous solution of oligosaccharide polymer such as cellulose and as a second phase a solution of a polymer such as a poly(ethylene glycol)-poly(propylene glycol) copolymer. The compound petitions into the first phase and binds to the oligosaccharide polymer, preferably with a Ka of 103 to 107, to form a complex. The complex is collected, and the compound is dissociated from the oligosaccharide polymer. The compound may be formed of a non-peptide chemical moiety or a peptide moiety linked to a polypeptide having the polysaccharide binding domain. The compound may also be a fusion polypeptide containing the polysaccharide binding domain linked through a protease recognition sequence to a macromolecule such as an enzyme, a hormone or an antibody. The macromolecule can be removed by using a protease to cleave the recognition sequence. Another partition system contains the oligosaccharide polymer and a phase separation inducing agent such as a sulfate or citrate salt that induces separation to produce different phases.

    摘要翻译： 具有多糖结合结构域的化合物，例如由纤维素包含并且基本上不含多糖酶活性的化合物，使用亲和分配系统从混合物中的其它成分中纯化。 将含有化合物的混合物与含有第一相的系统接触，例如纤维素等低聚糖聚合物的水溶液，作为第二相与聚（乙二醇） - 聚（丙二醇）共聚物等聚合物的溶液接触。 化合物进入第一阶段并结合寡糖聚合物，优选用103至107的Ka形成络合物。 收集该络合物，并且该化合物与寡糖聚合物解离。 化合物可以由非肽化学部分或与具有多糖结合结构域的多肽连接的肽部分形成。 化合物还可以是含有通过蛋白酶识别序列连接到大分子如酶，激素或抗体的多糖结合结构域的融合多肽。 可以通过使用蛋白酶切割识别序列来除去大分子。 另一分隔系统含有寡糖聚合物和相分离诱导剂如硫酸盐或柠檬酸盐，其诱导分离以产生不同相。

    Two-phase partition affinity separation system and affinity separated cell-containing composition
    7.
    发明授权
    Two-phase partition affinity separation system and affinity separated cell-containing composition 失效
    两相分离亲和分离系统和亲和力分离含细胞的组合物

    公开(公告)号：US6048715A

    公开(公告)日：2000-04-11

    申请号：US685808

    申请日：1996-07-24

    申请人： Charles A. Haynes ,  Peter Tomme ,  Douglas G. Kilburn

    发明人： Charles A. Haynes ,  Peter Tomme ,  Douglas G. Kilburn

    IPC分类号： A61K39/39 ,  C07K1/14 ,  C07K14/55 ,  C08B15/00 ,  C08B37/08 ,  C08H8/00 ,  C12N9/16 ,  C12N9/38 ,  C12N9/42 ,  C12N11/12 ,  C12P19/02 ,  C12Q1/56 ,  D21H17/00 ,  G01N33/531 ,  A23J1/00 ,  C12N15/00 ,  C12P21/06

    CPC分类号： C08H8/00 ,  A61K39/39 ,  C07K1/14 ,  C07K14/55 ,  C08B15/00 ,  C08B37/003 ,  C12N11/12 ,  C12N9/16 ,  C12N9/2437 ,  C12N9/2445 ,  C12P19/02 ,  C12Q1/56 ,  C12Y302/01004 ,  C12Y302/01021 ,  D21H17/005 ,  G01N33/531 ,  C07K2319/00 ,  G01N2333/924 ,  G01N2400/26 ,  Y10S435/803 ,  Y10S435/815 ,  Y10S530/813 ,  Y10S530/814

    摘要： A two-phase partition system is provided for affinity separation of a composition containing a polysaccharide binding peptide from a mixture such as a fermentation broth. The peptide may be from an enzyme and lacking in polysaccharidase activity such as the binding domain of cellulase that binds to cellulose. The system contains a phase-forming oligosaccharide polymer such as a cellulose derivative to which the peptide binds with a Ka of 10.sup.3 M to 10.sup.7 M, and a phase inducing agent such as a polyethylene glycol polymer, or a salt present at sufficiently high concentration to induce phase separation. If the oligosaccharide polymer is thermoseparating, phase separation can be induced by heating. Using the system involves contacting a composition containing the peptide such as a fusion protein with the system, partitioning the composition into a phase containing the oligosaccharide polymer by binding to the polymer and recovering the polymer containing the bound composition. The peptide or a fusion protein containing the peptide can be contacted with a cell having a carbohydrate residue to which the peptide binds to form a complex, and the complex is separated with the system to produce a bound cell composition. The peptide may be linked through a protease recognition sequence to a macromolecule such as an enzyme, a hormone or an antibody, and the macromolecule can be removed by using a protease to cleave the recognition sequence.

    摘要翻译： 提供了两相隔离系统，用于从包含发酵液的混合物中亲和分离含有多糖结合肽的组合物。 肽可以来自酶并且缺乏多糖酶活性，例如与纤维素结合的纤维素酶的结合结构域。 该体系含有形成型寡糖聚合物，例如纤维素衍生物，其肽与103M至107M的Ka结合，以及相诱导剂如聚乙二醇聚合物或以足够高的浓度存在的盐 诱导相分离。 如果寡糖聚合物是热分离的，则可以通过加热诱导相分离。 使用该系统包括使包含诸如融合蛋白的肽的组合物与系统接触，通过与聚合物结合并将含有结合的组合物的聚合物回收到含有寡糖聚合物的相中，将组合物分配。 可以将肽或含有该肽的融合蛋白与具有肽结合的碳水化合物残基的细胞接触以形成复合物，并且用体系分离复合物以产生结合的细胞组合物。 肽可以通过蛋白酶识别序列连接到大分子如酶，激素或抗体，并且可以通过使用蛋白酶来切割识别序列来除去大分子。

    Methods and compositions to promote bone homestasis
    9.
    发明申请
    Methods and compositions to promote bone homestasis 审中-公开
    促进骨质积聚的方法和组合物

    公开(公告)号：US20060020036A1

    公开(公告)日：2006-01-26

    申请号：US11166009

    申请日：2005-06-24

    申请人： Luc Van Rompaey ,  Peter Tomme

    发明人： Luc Van Rompaey ,  Peter Tomme

    IPC分类号： A61K31/18 ,  A61K31/195

    CPC分类号： G01N33/5073 ,  A61K31/18 ,  A61K31/195 ,  G01N33/6887 ,  G01N33/6893 ,  G01N33/76 ,  G01N2333/51 ,  G01N2333/726 ,  G01N2800/108

    摘要： The present invention relates to a method for promoting osteogenesis by contacting osteoblast progenitor cells with an LXR agonist. Said method is useful for the treatment or prevention of an imbalance in bone homeostasis in a subject using bone homeostasis-promoting compositions comprising an effective osteogenic stimulating amount of an LXR agonist in admixture with a pharmaceutically acceptable carrier. A further aspect is a method to produce bone tissue in vitro by contacting an LXR agonist with a population of osteoblast progenitor cells on a substrate, for a time sufficient to stimulate the generation of a matrix of bone tissue.

    摘要翻译： 本发明涉及通过将成骨细胞祖细胞与LXR激动剂接触来促进成骨的方法。 所述方法可用于治疗或预防使用骨稳态促进组合物治疗或预防受试者骨平衡的不平衡，所述组合物包含与药学上可接受的载体混合的有效成骨刺激量的LXR激动剂。 另一方面是通过将LXR激动剂与成骨细胞祖细胞群接触在基质上产生骨组织的方法，持续足以刺激骨组织基质的产生的时间。

    Methods and compositions to promote bone homeostasis
    10.
    发明申请
    Methods and compositions to promote bone homeostasis 审中-公开
    促进骨体内平衡的方法和组合物

    公开(公告)号：US20060014231A1

    公开(公告)日：2006-01-19

    申请号：US11166412

    申请日：2005-06-24

    申请人： Luc Van Rompaey ,  Peter Tomme

    发明人： Luc Van Rompaey ,  Peter Tomme

    IPC分类号： C12Q1/42

    CPC分类号： G01N33/5073 ,  A61K31/18 ,  A61K31/195 ,  G01N33/6887 ,  G01N33/6893 ,  G01N33/76 ,  G01N2333/51 ,  G01N2333/726 ,  G01N2800/108

    摘要： Methods are disclosed for identifying osteogenic promoting compounds by contacting test compounds with a target gene polypeptide or fragment thereof, which target gene is identified as involved in the osteogenesis process, and measuring a compound-polypeptide osteogenesis property. Also disclosed are methods of promoting osteogenesis by contacting progenitor cells with an effective osteogenic stimulating amount of an agonist of a target gene or an expressible nucleic acid of SEQ ID NO. 1-18, and may be used for the treatment or prevention of an imbalance in bone homeostasis. A further aspect is a method to produce bone tissue in vitro, by contacting a target gene agonist or an expressible nucleic acid of SEQ ID NO. 1-18 with a vertebrate cell population including osteoblast progenitor cells on a substrate.

    摘要翻译： 公开了通过使测试化合物与靶基因多肽或其片段接触来鉴定成骨促进化合物的方法，所述靶基因被鉴定为参与成骨过程，并测量化合物 - 多肽成骨性质。 还公开了通过使祖细胞与有效成骨刺激量的靶基因的激动剂或SEQ ID NO.1的可表达核酸接触来促进成骨的方法。 1-18，可用于治疗或预防骨平衡不稳定。 另一方面是通过使靶基因激动剂或可表达的核酸SEQ ID NO：1的接触，在体外产生骨组织的方法。 1-18具有脊椎动物细胞群，包括在底物上的成骨细胞祖细胞。