公开(公告)号：US3986556A

公开(公告)日：1976-10-19

申请号：US538591

申请日：1975-01-06

Applicant: Charles A. Haynes

Inventor： Charles A. Haynes

IPC: E21B36/02 ,  E21B43/243 ,  E21B43/40 ,  E21B43/24

CPC classification number: E21B36/02 ,  E21B43/243 ,  E21B43/40

Abstract: A catalytic process for stimulating the recovery of hydrocarbons from porous and permeable hydrocarbon bearing strata in earth formations utlizing at least one injection well and at least one production well both completed in said hydrocarbon bearing strata and a finely divided hydrocarbon cracking catalyst delivered to said strata by an injection gas controlled at suitable pressure, temperature and O.sub.2 content to promote and advance in place catalytic cracking of hydrocarbons and catalyst regeneration within said strata to be produced.

Abstract translation: 一种用于刺激从地层中的多孔和可渗透含烃层回收烃的催化方法，其利用至少一个注入井和至少一个在所述含烃地层中完成的生产井和通过以下方式递送到所述地层的细碎烃裂解催化剂： 控制在合适的压力，温度和O2含量的注入气体，以促进并推进烃的催化裂化和所生成的所述地层内的催化剂再生。

公开(公告)号：US20120015347A1

公开(公告)日：2012-01-19

申请号：US13184363

申请日：2011-07-15

Applicant: Anupam Singhal ,  Carl L. G. Hansen ,  John W. Schrader ,  Charles A. Haynes ,  Daniel J. Da Costa

Inventor： Anupam Singhal ,  Carl L. G. Hansen ,  John W. Schrader ,  Charles A. Haynes ,  Daniel J. Da Costa

IPC: C12Q1/70 ,  C12M1/34 ,  C12Q1/68 ,  G01N33/566 ,  G01N33/577

CPC classification number: G01N33/56966 ,  B01L3/502738 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0681 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0481 ,  G01N33/577 ,  G01N33/582 ,  G01N33/6854

Abstract: There are provided methods, and devices for assaying for a binding interaction between a protein, such as a monoclonal antibody, produced by a cell, and a biomolecule. The method may include retaining the cell within a chamber having an aperture; exposing the protein produced by the cell to a capture substrate, wherein the capture substrate is in fluid communication with the protein produced by the cell and wherein the capture substrate is operable to bind the protein produced by the cell; flowing a fluid volume comprising the biomolecule through the chamber via said aperture, wherein the fluid volume is in fluid communication with the capture substrate; and determining a binding interaction between the protein produced by the cell and the biomolecule.

Abstract translation: 提供了用于测定由细胞产生的蛋白质（例如单克隆抗体）和生物分子之间的结合相互作用的方法和装置。 该方法可以包括将细胞保持在具有孔的腔室内; 将由细胞产生的蛋白质暴露于捕获底物，其中所述捕获底物与由所述细胞产生的蛋白质流体连通，并且其中所述捕获底物可操作地结合由所述细胞产生的蛋白质; 使包含所述生物分子的流体体积经由所述孔流过所述腔室，其中所述流体体积与所述捕获衬底流体连通; 并确定由细胞产生的蛋白质与生物分子之间的结合相互作用。

公开(公告)号：US06174700B1

公开(公告)日：2001-01-16

申请号：US08505860

申请日：1995-07-24

Applicant: Charles A. Haynes ,  Peter Tomme ,  Douglas G. Kilburn

Inventor： Charles A. Haynes ,  Peter Tomme ,  Douglas G. Kilburn

IPC: C12P2106

CPC classification number: A61K39/39 ,  C07K1/14 ,  C07K14/55 ,  C07K2319/00 ,  C08B15/00 ,  C08B37/003 ,  C08H8/00 ,  C12N9/16 ,  C12N9/2437 ,  C12N9/2445 ,  C12N11/12 ,  C12P19/02 ,  C12Q1/56 ,  C12Y302/01004 ,  C12Y302/01021 ,  D21H17/005 ,  G01N33/531 ,  G01N2333/924 ,  G01N2400/26 ,  Y10S435/803 ,  Y10S435/815 ,  Y10S530/813 ,  Y10S530/814

Abstract: A compound having a polysaccharide binding domain such as contained by a cellulose and essentially lacking in polysaccharidase activity is purified from other ingredients in a mixture using an affinity partition system. A mixture containing the compound is contacted with a system containing as a first phase an aqueous solution of oligosaccharide polymer such as cellulose and as a second phase a solution of a polymer such as a poly(ethylene glycol)-poly(propylene glycol) copolymer. The compound petitions into the first phase and binds to the oligosaccharide polymer, preferably with a Ka of 103 to 107, to form a complex. The complex is collected, and the compound is dissociated from the oligosaccharide polymer. The compound may be formed of a non-peptide chemical moiety or a peptide moiety linked to a polypeptide having the polysaccharide binding domain. The compound may also be a fusion polypeptide containing the polysaccharide binding domain linked through a protease recognition sequence to a macromolecule such as an enzyme, a hormone or an antibody. The macromolecule can be removed by using a protease to cleave the recognition sequence. Another partition system contains the oligosaccharide polymer and a phase separation inducing agent such as a sulfate or citrate salt that induces separation to produce different phases.

Abstract translation: 具有多糖结合结构域的化合物，例如由纤维素包含并且基本上不含多糖酶活性的化合物，使用亲和分配系统从混合物中的其它成分中纯化。 将含有化合物的混合物与含有第一相的系统接触，例如纤维素等低聚糖聚合物的水溶液，作为第二相与聚（乙二醇） - 聚（丙二醇）共聚物等聚合物的溶液接触。 化合物进入第一阶段并结合寡糖聚合物，优选用103至107的Ka形成络合物。 收集该络合物，并且该化合物与寡糖聚合物解离。 化合物可以由非肽化学部分或与具有多糖结合结构域的多肽连接的肽部分形成。 化合物还可以是含有通过蛋白酶识别序列连接到大分子如酶，激素或抗体的多糖结合结构域的融合多肽。 可以通过使用蛋白酶切割识别序列来除去大分子。 另一分隔系统含有寡糖聚合物和相分离诱导剂如硫酸盐或柠檬酸盐，其诱导分离以产生不同相。

公开(公告)号：US09188593B2

公开(公告)日：2015-11-17

申请号：US13184363

申请日：2011-07-15

Applicant: Anupam Singhal ,  Carl L. G. Hansen ,  John W. Schrader ,  Charles A. Haynes ,  Daniel J. Da Costa

Inventor： Anupam Singhal ,  Carl L. G. Hansen ,  John W. Schrader ,  Charles A. Haynes ,  Daniel J. Da Costa

IPC: B01L3/00 ,  G01N33/68 ,  G01N33/569

CPC classification number: G01N33/56966 ,  B01L3/502738 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0681 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0481 ,  G01N33/577 ,  G01N33/582 ,  G01N33/6854

Abstract: There are provided methods, and devices for assaying for a binding interaction between a protein, such as a monoclonal antibody, produced by a cell, and a biomolecule. The method may include retaining the cell within a chamber having an aperture; exposing the protein produced by the cell to a capture substrate, wherein the capture substrate is in fluid communication with the protein produced by the cell and wherein the capture substrate is operable to bind the protein produced by the cell; flowing a fluid volume comprising the biomolecule through the chamber via said aperture, wherein the fluid volume is in fluid communication with the capture substrate; and determining a binding interaction between the protein produced by the cell and the biomolecule.

Abstract translation: 提供了用于测定由细胞产生的蛋白质（例如单克隆抗体）和生物分子之间的结合相互作用的方法和装置。 该方法可以包括将细胞保持在具有孔的腔室内; 将由细胞产生的蛋白质暴露于捕获底物，其中所述捕获底物与由所述细胞产生的蛋白质流体连通，并且其中所述捕获底物可操作地结合由所述细胞产生的蛋白质; 使包含所述生物分子的流体体积经由所述孔流过所述腔室，其中所述流体体积与所述捕获衬底流体连通; 并确定由细胞产生的蛋白质与生物分子之间的结合相互作用。

公开(公告)号：US6096119A

公开(公告)日：2000-08-01

申请号：US114906

申请日：1998-07-14

Applicant: Chi-Pai Ho ,  Ronald D. Voigts ,  Charles A. Haynes ,  James S. Blair

Inventor： Chi-Pai Ho ,  Ronald D. Voigts ,  Charles A. Haynes ,  James S. Blair

IPC: B03C3/08 ,  B03C3/12 ,  B03C3/60 ,  B03C3/68 ,  B03C3/72 ,  B03C3/86

CPC classification number: B03C3/72 ,  B03C3/12 ,  B03C3/86

Abstract: In a two-stage, electrostatic precipitator for extracting airborne particles, charged plates are electrically connected to each other and physically separated from each other by ferrite spacers so that an impedance of the spacers limits an amount of arc discharge current that will flow when an arc discharge occurs from one of the charged plates. Ferrite spacers can also be provided to electrically connect and physically separate grounded plates in the precipitator. In addition, aluminum spacers can be provided to adjust the impedance of the series of spacers through which an arc discharge current flows, so that the current is a minimum necessary amount for an arc detection circuit to detect.

Abstract translation: 在用于提取空气中的颗粒的两级静电除尘器中，带电板彼此电连接并且通过铁氧体间隔物彼此物理分离，使得间隔物的阻抗限制了当弧形时将流动的电弧放电电流的量 从一个充电板发生放电。 也可以提供铁氧体间隔件，以电连接和物理分离除尘器中的接地板。 此外，可以提供铝间隔物以调节电弧放电电流流过的一系列间隔件的阻抗，使得电流是电弧检测电路检测的最小必要量。

公开(公告)号：US6048715A

公开(公告)日：2000-04-11

申请号：US685808

申请日：1996-07-24

Applicant: Charles A. Haynes ,  Peter Tomme ,  Douglas G. Kilburn

Inventor： Charles A. Haynes ,  Peter Tomme ,  Douglas G. Kilburn

IPC: A61K39/39 ,  C07K1/14 ,  C07K14/55 ,  C08B15/00 ,  C08B37/08 ,  C08H8/00 ,  C12N9/16 ,  C12N9/38 ,  C12N9/42 ,  C12N11/12 ,  C12P19/02 ,  C12Q1/56 ,  D21H17/00 ,  G01N33/531 ,  A23J1/00 ,  C12N15/00 ,  C12P21/06

CPC classification number: C08H8/00 ,  A61K39/39 ,  C07K1/14 ,  C07K14/55 ,  C08B15/00 ,  C08B37/003 ,  C12N11/12 ,  C12N9/16 ,  C12N9/2437 ,  C12N9/2445 ,  C12P19/02 ,  C12Q1/56 ,  C12Y302/01004 ,  C12Y302/01021 ,  D21H17/005 ,  G01N33/531 ,  C07K2319/00 ,  G01N2333/924 ,  G01N2400/26 ,  Y10S435/803 ,  Y10S435/815 ,  Y10S530/813 ,  Y10S530/814

Abstract: A two-phase partition system is provided for affinity separation of a composition containing a polysaccharide binding peptide from a mixture such as a fermentation broth. The peptide may be from an enzyme and lacking in polysaccharidase activity such as the binding domain of cellulase that binds to cellulose. The system contains a phase-forming oligosaccharide polymer such as a cellulose derivative to which the peptide binds with a Ka of 10.sup.3 M to 10.sup.7 M, and a phase inducing agent such as a polyethylene glycol polymer, or a salt present at sufficiently high concentration to induce phase separation. If the oligosaccharide polymer is thermoseparating, phase separation can be induced by heating. Using the system involves contacting a composition containing the peptide such as a fusion protein with the system, partitioning the composition into a phase containing the oligosaccharide polymer by binding to the polymer and recovering the polymer containing the bound composition. The peptide or a fusion protein containing the peptide can be contacted with a cell having a carbohydrate residue to which the peptide binds to form a complex, and the complex is separated with the system to produce a bound cell composition. The peptide may be linked through a protease recognition sequence to a macromolecule such as an enzyme, a hormone or an antibody, and the macromolecule can be removed by using a protease to cleave the recognition sequence.

Abstract translation: 提供了两相隔离系统，用于从包含发酵液的混合物中亲和分离含有多糖结合肽的组合物。 肽可以来自酶并且缺乏多糖酶活性，例如与纤维素结合的纤维素酶的结合结构域。 该体系含有形成型寡糖聚合物，例如纤维素衍生物，其肽与103M至107M的Ka结合，以及相诱导剂如聚乙二醇聚合物或以足够高的浓度存在的盐 诱导相分离。 如果寡糖聚合物是热分离的，则可以通过加热诱导相分离。 使用该系统包括使包含诸如融合蛋白的肽的组合物与系统接触，通过与聚合物结合并将含有结合的组合物的聚合物回收到含有寡糖聚合物的相中，将组合物分配。 可以将肽或含有该肽的融合蛋白与具有肽结合的碳水化合物残基的细胞接触以形成复合物，并且用体系分离复合物以产生结合的细胞组合物。 肽可以通过蛋白酶识别序列连接到大分子如酶，激素或抗体，并且可以通过使用蛋白酶来切割识别序列来除去大分子。

公开(公告)号：US4528441A

公开(公告)日：1985-07-09

申请号：US561264

申请日：1983-12-14

Applicant: Douglas T. Seal ,  Charles A. Haynes

Inventor： Douglas T. Seal ,  Charles A. Haynes

IPC: H05B3/16 ,  H05B3/06

CPC classification number: H05B3/16 ,  Y10T29/49083 ,  Y10T29/49872 ,  Y10T29/49876 ,  Y10T29/53657

Abstract: An electrical resistance heating assembly having specially designed electrical insulators is disclosed. The electrical insulators have arms shaped to retain an electrical resistance heating coil which can be quickly, easily, and reliably mounted on the arms of the insulator. Top parts of the arms are generally rectangular or triangular in cross section and the arms are spaced and configured relative to each other to form a generally T-shaped opening between the arms, with the ends of the cross bar of the T-shaped opening forming electrically resistance heating coil retention surfaces. Also, cuts in side surfaces of the insulators may provide additional coil retention surfaces. The insulators have either an extended base portion for directly mounting the insulators to a mounting bar of the electrical resistance heating assembly or the insulators simply comprise slabs of electrically insulating material which are each mounted on a cross bar which is attached to a mounting bar of the electrical resistance heating assembly.

Abstract translation: 公开了一种具有专门设计的电绝缘体的电阻加热组件。 电绝缘体具有形状以保持能够快速，容易且可靠地安装在绝缘体的臂上的电阻加热线圈的臂。 臂的顶部横截面通常为矩形或三角形，并且臂相对于彼此间隔开并构造成在臂之间形成大致T形的开口，T形开口的横杆的端部形成 电阻加热线圈保持面。 此外，绝缘体的侧表面的切割可以提供附加的线圈保持表面。 绝缘体具有用于将绝缘体直接安装到电阻加热组件的安装杆的扩展基部，或者绝缘体简单地包括电绝缘材料板，它们分别安装在横杆上，该横杆连接到 电阻加热总成。

公开(公告)号：US4086960A

公开(公告)日：1978-05-02

申请号：US661325

申请日：1976-02-25

Applicant: Charles A. Haynes

Inventor： Charles A. Haynes

IPC: E21B36/02 ,  E21B43/243 ,  E21B43/40 ,  E21B43/24

CPC classification number: E21B36/02 ,  E21B43/243 ,  E21B43/40

Abstract: A combination structure adapted for use in a catalytic process for stimulating the recovery of hydrocarbons from porous and permeable hydrocarbon bearing strata in earth formations utilizing at least one injection well and at least one production well both completed in said hydrocarbon bearing strata and a finely divided hydrocarbon cracking catalyst which is delivered by and through said structural combination of parts and elements to said strata by injection gas controlled at suitable pressure, temperature and O.sub.2 content to promote and advance in place catalytic cracking of hydrocarbons and catalyst regeneration within said strata to be produced by utilizing said combination structure or its equivalent.

Abstract translation: 适用于催化过程的组合结构，其利用至少一个注入井和至少一个在所述含烃层中完成的至少一个生产井来刺激从地层中的多孔和可渗透烃承载层回收烃，以及细分烃 裂解催化剂，其由部分和元素的所述结构组合输送到所述地层，通过在合适的压力，温度和O 2含量下控制的注入气体来促进并推进烃的催化裂化和所述层内的催化剂再生以由 利用所述组合结构或其等同物。

公开(公告)号：US5998173A

公开(公告)日：1999-12-07

申请号：US603360

申请日：1996-02-20

Applicant: Charles A. Haynes ,  Pedro Aloise ,  Aimee Louise Creagh

Inventor： Charles A. Haynes ,  Pedro Aloise ,  Aimee Louise Creagh

IPC: C12N9/24 ,  C12P19/26 ,  C12P19/28 ,  C12R1/645

CPC classification number: C12Y302/01096 ,  C12N9/2442 ,  C12P19/26 ,  C12Y302/01014 ,  Y10S435/822 ,  Y10S435/881 ,  Y10S435/911 ,  Y10S435/945

Abstract: This invention pertains to a novel process for directly producing N-acetyl-D-glucosamine from chitin. More particularly, this invention pertains to a novel process for producing N-acetyl-D-glucosamine utilizing an ensemble of the chitinase family of enzymes to hydrolyze chitin of crustacea shells. The invention includes a process for producing N-acetyl-D-glucosamine by enzymatically hydrolyzing chitin with an ensemble of chitinolytic enzymes, including chitinase and chitobiase. In particular, using a two-stage chitin-hydrolysis reactor.

Abstract translation: 本发明涉及从几丁质中直接生产N-乙酰基-D-葡糖胺的新方法。 更具体地说，本发明涉及利用酶的几丁质酶家族的组合产生N-乙酰基-D-葡糖胺的新方法，以水解壳甲壳素的几丁质。 本发明包括通过用包含几丁质酶和木质素酶的chitinotictic酶的整体酶促水解壳多糖来生产N-乙酰基-D-葡糖胺的方法。 特别是使用两阶段甲壳质水解反应器。

公开(公告)号：US5198003A

公开(公告)日：1993-03-30

申请号：US724650

申请日：1991-07-02

Applicant: Charles A. Haynes

Inventor： Charles A. Haynes

IPC: B03C3/08 ,  B03C3/12 ,  B03C3/86

CPC classification number: B03C3/86 ,  B03C3/08 ,  B03C3/12 ,  Y10S264/48 ,  Y10T29/49195

Abstract: An electrostatic air cleaner collector and/or ionizer section has their oppositely charged elements being formed of single, continuous, electrically conductive elements, spirally wound around insulator rods to jointly define a spiral passage through which air is caused to flow. The collector and ionizer may be assembled separately by such a winding process, or they may contain common insulator rods around which the conductive element of both sections may be simultaneously wound. A conductive rod may be inserted to electrically interconnect the high voltage elements of the respective ionizer and collector sections.

Abstract translation: 静电空气滤清器收集器和/或电离器部分具有相对充电元件，由单个，连续的导电元件形成，螺旋地卷绕在绝缘杆周围，以共同限定使空气流过的螺旋通道。 收集器和离子发生器可以通过这种卷绕过程分开组装，或者它们可以包含公共绝缘体棒，两个部分的导电元件可以同时缠绕在该绝缘杆周围。 可以插入导电棒以将相应的电离器和收集器部分的高电压元件电互连。