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17 条记录 (耗时 0.016 秒)

    Composition for detecting β-1,3-glucan
    4.
    发明授权
    Composition for detecting β-1,3-glucan 失效
    检测β-1,3-葡聚糖的组合物

    公开(公告)号：US06987002B2

    公开(公告)日：2006-01-17

    申请号：US09938334

    申请日：2001-08-23

    申请人： Joong Hyuck Auh ,  Bu Soo Park ,  Chang Hun Joo ,  Chong Jin Park ,  Bok Luel Lee ,  Kum Young Lee ,  Seung-Suh Hong ,  Hyun-Soo Lee

    发明人： Joong Hyuck Auh ,  Bu Soo Park ,  Chang Hun Joo ,  Chong Jin Park ,  Bok Luel Lee ,  Kum Young Lee ,  Seung-Suh Hong ,  Hyun-Soo Lee

    IPC分类号： C12Q1/26

    CPC分类号： C12Q1/26

    摘要： The present invention relates to a composition for detecting an infinitesimal quantity of β-1,3-glucan, a preparation method thereof and a diagnostic kit detecting β-1,3-glucan. The composition of the present invention shows phenoloxidase activity by β-1,3-glucan in the presence of calcium ions. Using the composition of the present invention, a sample is gathered from a specimen, the composition of the present invention and calcium ions are added to the sample, and β-1,3-glucan is detected by measuring phenoloxidase activity.

    摘要翻译： 本发明涉及一种用于检测β-1,3-葡聚糖无穷小量的组合物，其制备方法和检测β-1,3-葡聚糖的诊断试剂盒。 本发明的组合物在钙离子存在下显示β-1,3-葡聚糖的酚氧化酶活性。 使用本发明的组合物，从样品中收集样品，将本发明的组合物和钙离子加入到样品中，通过测量酚氧化酶活性来检测β-1,3-葡聚糖。

    Mass production method of antimicrobial peptide and DNA construct and expression system thereof
    5.
    发明授权
    Mass production method of antimicrobial peptide and DNA construct and expression system thereof 有权
    抗菌肽和DNA构建体的大规模生产方法及其表达系统

    公开(公告)号：US06699689B1

    公开(公告)日：2004-03-02

    申请号：US09485147

    申请日：2000-04-25

    申请人： Jeong Hyun Kim ,  Min Hyung Kang ,  Jae Hyun Lee ,  Se Ho Park ,  Joo Won Lee ,  Seung Suh Hong ,  Hyun Soo Lee

    发明人： Jeong Hyun Kim ,  Min Hyung Kang ,  Jae Hyun Lee ,  Se Ho Park ,  Joo Won Lee ,  Seung Suh Hong ,  Hyun Soo Lee

    IPC分类号： C12P2106

    CPC分类号： C12N15/72 ,  C07K14/4723 ,  C07K2319/00 ,  C12N9/1077 ,  C12N15/70

    摘要： The present invention relates to DNA constructs that can produce antimicrobial materials efficiently from microorganisms and the preparation method thereof. The present invention also relates to the useful vector for the DNA construct. The DNA construct according to the present invention comprises a first gene coding for entire, a part of or a derivative of purF gene and a second gene coding for antimicrobial peptide. According to the present invention, antimicrobial peptides can be mass-produced by the following steps: preparing an expression vector containing a DNA construct comprising a first gene coding for an entire, a part of or a derivative of purF gene and a second gene coding for antimicrobial peptide; transforming the bacterial host cells with the above-mentioned vector, culturing the transformed cell to express the above-mentioned DNA construct; and recovering the above antimicrobial peptide.

    摘要翻译： 本发明涉及能够从微生物有效生产抗微生物材料的DNA构建体及其制备方法。 本发明还涉及DNA构建体的有用载体。 根据本发明的DNA构建体包含编码purF基因的全部，一部分或衍生物的第一个基因和编码抗微生物肽的第二个基因。 根据本发明，通过以下步骤可以大量生产抗微生物肽：制备含有DNA构建体的表达载体，所述构建体包含编码purF基因全部或部分或衍生物的第一个基因，以及编码 抗菌肽; 用上述载体转化细菌宿主细胞，培养转化细胞以表达上述DNA构建体; 并回收上述抗微生物肽。

    Method for mass production of antimicrobial peptide
    7.
    发明授权
    Method for mass production of antimicrobial peptide 有权
    大量生产抗菌肽的方法

    公开(公告)号：US06183992B2

    公开(公告)日：2001-02-06

    申请号：US09230180

    申请日：1999-03-10

    申请人： Sun-Chang Kim ,  Jae Hyun Lee ,  Min Hyung Kang ,  Jeong Hyun Kim ,  Seung-Suh Hong ,  Hyun-Soo Lee

    发明人： Sun-Chang Kim ,  Jae Hyun Lee ,  Min Hyung Kang ,  Jeong Hyun Kim ,  Seung-Suh Hong ,  Hyun-Soo Lee

    IPC分类号： C12P2106

    CPC分类号： C07K7/08 ,  C07K14/43563 ,  C07K14/43572 ,  C07K14/46 ,  C07K14/4723 ,  C07K14/815 ,  C07K2319/00 ,  C07K2319/23 ,  C12N15/62

    摘要： The present invention provides a method for mass production of an antimicrobial peptide, which comprises the steps of: constructing a fusion gene containing a first gene encoding a negatively charged acidic peptide having at least two cysteine residues and a second gene encoding a positively charged basic antimicrobial peptide; transforming a host microorganism with an expression vector comprising the fusion gene; cultivating the transformed microorganism to express a fusion peptide containing the acidic peptide and antimicrobial peptide, and, recovering the expressed antimicrobial peptide. In accordance with the present invention, the inhibitory effect of the expressed antimicrobial peptide on the growth of the host microorganism can be dramatically minimized by fusing it with the acidic peptide. Accordingly, antimicrobial peptides can be produced massively from a recombinant microorganism regardless of the kind of the antimicrobial peptides.

    摘要翻译： 本发明提供了一种大规模生产抗微生物肽的方法，其包括以下步骤：构建含有编码带有至少两个半胱氨酸残基的带负电荷的酸性肽的第一基因和编码带正电荷的碱性抗微生物剂的第二基因的融合基因 肽; 用包含融合基因的表达载体转化宿主微生物; 培养转化的微生物以表达含有酸性肽和抗微生物肽的融合肽，并回收表达的抗微生物肽。 根据本发明，表达的抗微生物肽对宿主微生物的生长的抑制作用可以通过与酸性肽融合来显着地最小化。 因此，抗微生物肽可以从重组微生物大量生产，而不管抗微生物肽的种类。

    Ice nucleation active microorganism
    8.
    发明授权
    Ice nucleation active microorganism 失效
    冰核活性微生物

    公开(公告)号：US5972686A

    公开(公告)日：1999-10-26

    申请号：US77218

    申请日：1998-05-19

    申请人： Choong-Kyung Kang ,  Gwang-Hwee Na ,  Hyun-Geun Yoon ,  Seung-Suh Hong ,  Hyun-Soo Lee

    发明人： Choong-Kyung Kang ,  Gwang-Hwee Na ,  Hyun-Geun Yoon ,  Seung-Suh Hong ,  Hyun-Soo Lee

    IPC分类号： F25C1/00 ,  A23G9/52 ,  A23L3/37 ,  A23L3/44 ,  C12N1/20

    CPC分类号： C12R1/64 ,  A23G9/52 ,  A23L3/37 ,  A23L3/44 ,  A23G2200/02 ,  Y10S435/91

    摘要： The present invention relates to a novel ice nucleation active Xanthomonas strain and a bacterial ice nucleator comprising the ice nucleation active microorganism which can be applied for food processing and artificial snow making. The present inventors have screened ice nucleation active microorganisms from leaves of crops and plants, and investigated their ice-nucleation activities. As a result, the inventors discovered that a novel microorganism belonging to Xanthomonas campestris has a superior ice-nucleation activity than those of the conventional ice nucleation active microorganisms. Accordingly, the ice nucleation active microorganism of the invention can be used as a potent bacterial ice nucleator for food processing and artificial snow making.

    摘要翻译： PCT No.PCT / KR96 / 00254 Sec。 371日期1998年5月19日 102（e）日期1998年5月19日PCT提交1996年12月27日PCT公布。 WO98 / 12301 PCT出版物 日期：1998年5月26日本发明涉及一种新颖的冰核活性黄单胞菌属菌株和含有冰核活性微生物的细菌冰核子，其可应用于食品加工和人造雪。 本发明人从作物和植物的叶片中筛选出了冰核活性微生物，并研究了它们的冰 - 成核活性。 结果发现，属于野油菜黄单胞菌属的新型微生物具有优于常规冰核活性微生物的冰成核活性。 因此，本发明的冰核活性微生物可以用作食品加工和人造雪的有效的细菌冰核。

    Method for removing endotoxin from the samples containing basic protein
    9.
    发明授权
    Method for removing endotoxin from the samples containing basic protein 失效
    从含有碱性蛋白质的样品中去除内毒素的方法

    公开(公告)号：US06713611B2

    公开(公告)日：2004-03-30

    申请号：US09948491

    申请日：2001-09-06

    申请人： Sang-Hyun Pyo ,  Heung-Bok Park ,  Jin-Eon So ,  Jin-Hyun Kim ,  Seung-Suh Hong ,  Hyun-Soo Lee

    发明人： Sang-Hyun Pyo ,  Heung-Bok Park ,  Jin-Eon So ,  Jin-Hyun Kim ,  Seung-Suh Hong ,  Hyun-Soo Lee

    IPC分类号： C07K118

    CPC分类号： C07K1/18

    摘要： The present invention relates to a method of removing an endotoxin from solution containing basic proteins. More specifically, the method of the present invention comprises the steps of adding the surfactant to solution containing the basic protein obtained from the recombinant microorganism and mixing the resultant, of loading the resultant solution on the cation exchange column, washing the cation exchange column with solution which does not contain surfactant, and eluting the basic protein of interest from the cation exchange column.

    摘要翻译： 本发明涉及从含有碱性蛋白质的溶液中除去内毒素的方法。 更具体地说，本发明的方法包括以下步骤：将表面活性剂加入含有由重组微生物获得的碱性蛋白质的溶液中，并将所得溶液混合，将所得溶液装载到阳离子交换柱上，用溶液洗涤阳离子交换柱 其不含表面活性剂，并从阳离子交换柱洗脱出所需的碱性蛋白质。