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13 条记录 (耗时 0.027 秒)

    Ice nucleation active microorganism
    1.
    发明授权
    Ice nucleation active microorganism 失效
    冰核活性微生物

    公开(公告)号：US5972686A

    公开(公告)日：1999-10-26

    申请号：US77218

    申请日：1998-05-19

    申请人： Choong-Kyung Kang ,  Gwang-Hwee Na ,  Hyun-Geun Yoon ,  Seung-Suh Hong ,  Hyun-Soo Lee

    发明人： Choong-Kyung Kang ,  Gwang-Hwee Na ,  Hyun-Geun Yoon ,  Seung-Suh Hong ,  Hyun-Soo Lee

    IPC分类号： F25C1/00 ,  A23G9/52 ,  A23L3/37 ,  A23L3/44 ,  C12N1/20

    CPC分类号： C12R1/64 ,  A23G9/52 ,  A23L3/37 ,  A23L3/44 ,  A23G2200/02 ,  Y10S435/91

    摘要： The present invention relates to a novel ice nucleation active Xanthomonas strain and a bacterial ice nucleator comprising the ice nucleation active microorganism which can be applied for food processing and artificial snow making. The present inventors have screened ice nucleation active microorganisms from leaves of crops and plants, and investigated their ice-nucleation activities. As a result, the inventors discovered that a novel microorganism belonging to Xanthomonas campestris has a superior ice-nucleation activity than those of the conventional ice nucleation active microorganisms. Accordingly, the ice nucleation active microorganism of the invention can be used as a potent bacterial ice nucleator for food processing and artificial snow making.

    摘要翻译： PCT No.PCT / KR96 / 00254 Sec。 371日期1998年5月19日 102（e）日期1998年5月19日PCT提交1996年12月27日PCT公布。 WO98 / 12301 PCT出版物 日期：1998年5月26日本发明涉及一种新颖的冰核活性黄单胞菌属菌株和含有冰核活性微生物的细菌冰核子，其可应用于食品加工和人造雪。 本发明人从作物和植物的叶片中筛选出了冰核活性微生物，并研究了它们的冰 - 成核活性。 结果发现，属于野油菜黄单胞菌属的新型微生物具有优于常规冰核活性微生物的冰成核活性。 因此，本发明的冰核活性微生物可以用作食品加工和人造雪的有效的细菌冰核。

    Method for removing endotoxin from the samples containing basic protein
    2.
    发明授权
    Method for removing endotoxin from the samples containing basic protein 失效
    从含有碱性蛋白质的样品中去除内毒素的方法

    公开(公告)号：US06713611B2

    公开(公告)日：2004-03-30

    申请号：US09948491

    申请日：2001-09-06

    申请人： Sang-Hyun Pyo ,  Heung-Bok Park ,  Jin-Eon So ,  Jin-Hyun Kim ,  Seung-Suh Hong ,  Hyun-Soo Lee

    发明人： Sang-Hyun Pyo ,  Heung-Bok Park ,  Jin-Eon So ,  Jin-Hyun Kim ,  Seung-Suh Hong ,  Hyun-Soo Lee

    IPC分类号： C07K118

    CPC分类号： C07K1/18

    摘要： The present invention relates to a method of removing an endotoxin from solution containing basic proteins. More specifically, the method of the present invention comprises the steps of adding the surfactant to solution containing the basic protein obtained from the recombinant microorganism and mixing the resultant, of loading the resultant solution on the cation exchange column, washing the cation exchange column with solution which does not contain surfactant, and eluting the basic protein of interest from the cation exchange column.

    摘要翻译： 本发明涉及从含有碱性蛋白质的溶液中除去内毒素的方法。 更具体地说，本发明的方法包括以下步骤：将表面活性剂加入含有由重组微生物获得的碱性蛋白质的溶液中，并将所得溶液混合，将所得溶液装载到阳离子交换柱上，用溶液洗涤阳离子交换柱 其不含表面活性剂，并从阳离子交换柱洗脱出所需的碱性蛋白质。

    Composition for detecting β-1,3-glucan
    5.
    发明授权
    Composition for detecting β-1,3-glucan 失效
    检测β-1,3-葡聚糖的组合物

    公开(公告)号：US06987002B2

    公开(公告)日：2006-01-17

    申请号：US09938334

    申请日：2001-08-23

    申请人： Joong Hyuck Auh ,  Bu Soo Park ,  Chang Hun Joo ,  Chong Jin Park ,  Bok Luel Lee ,  Kum Young Lee ,  Seung-Suh Hong ,  Hyun-Soo Lee

    发明人： Joong Hyuck Auh ,  Bu Soo Park ,  Chang Hun Joo ,  Chong Jin Park ,  Bok Luel Lee ,  Kum Young Lee ,  Seung-Suh Hong ,  Hyun-Soo Lee

    IPC分类号： C12Q1/26

    CPC分类号： C12Q1/26

    摘要： The present invention relates to a composition for detecting an infinitesimal quantity of β-1,3-glucan, a preparation method thereof and a diagnostic kit detecting β-1,3-glucan. The composition of the present invention shows phenoloxidase activity by β-1,3-glucan in the presence of calcium ions. Using the composition of the present invention, a sample is gathered from a specimen, the composition of the present invention and calcium ions are added to the sample, and β-1,3-glucan is detected by measuring phenoloxidase activity.

    摘要翻译： 本发明涉及一种用于检测β-1,3-葡聚糖无穷小量的组合物，其制备方法和检测β-1,3-葡聚糖的诊断试剂盒。 本发明的组合物在钙离子存在下显示β-1,3-葡聚糖的酚氧化酶活性。 使用本发明的组合物，从样品中收集样品，将本发明的组合物和钙离子加入到样品中，通过测量酚氧化酶活性来检测β-1,3-葡聚糖。

    Method for mass production of antimicrobial peptide
    6.
    发明授权
    Method for mass production of antimicrobial peptide 有权
    大量生产抗菌肽的方法

    公开(公告)号：US06183992B2

    公开(公告)日：2001-02-06

    申请号：US09230180

    申请日：1999-03-10

    申请人： Sun-Chang Kim ,  Jae Hyun Lee ,  Min Hyung Kang ,  Jeong Hyun Kim ,  Seung-Suh Hong ,  Hyun-Soo Lee

    发明人： Sun-Chang Kim ,  Jae Hyun Lee ,  Min Hyung Kang ,  Jeong Hyun Kim ,  Seung-Suh Hong ,  Hyun-Soo Lee

    IPC分类号： C12P2106

    CPC分类号： C07K7/08 ,  C07K14/43563 ,  C07K14/43572 ,  C07K14/46 ,  C07K14/4723 ,  C07K14/815 ,  C07K2319/00 ,  C07K2319/23 ,  C12N15/62

    摘要： The present invention provides a method for mass production of an antimicrobial peptide, which comprises the steps of: constructing a fusion gene containing a first gene encoding a negatively charged acidic peptide having at least two cysteine residues and a second gene encoding a positively charged basic antimicrobial peptide; transforming a host microorganism with an expression vector comprising the fusion gene; cultivating the transformed microorganism to express a fusion peptide containing the acidic peptide and antimicrobial peptide, and, recovering the expressed antimicrobial peptide. In accordance with the present invention, the inhibitory effect of the expressed antimicrobial peptide on the growth of the host microorganism can be dramatically minimized by fusing it with the acidic peptide. Accordingly, antimicrobial peptides can be produced massively from a recombinant microorganism regardless of the kind of the antimicrobial peptides.

    摘要翻译： 本发明提供了一种大规模生产抗微生物肽的方法，其包括以下步骤：构建含有编码带有至少两个半胱氨酸残基的带负电荷的酸性肽的第一基因和编码带正电荷的碱性抗微生物剂的第二基因的融合基因 肽; 用包含融合基因的表达载体转化宿主微生物; 培养转化的微生物以表达含有酸性肽和抗微生物肽的融合肽，并回收表达的抗微生物肽。 根据本发明，表达的抗微生物肽对宿主微生物的生长的抑制作用可以通过与酸性肽融合来显着地最小化。 因此，抗微生物肽可以从重组微生物大量生产，而不管抗微生物肽的种类。

    Mass production of paclitaxel by changing the temperature of the medium during the plant cell culture
    7.
    发明授权
    Mass production of paclitaxel by changing the temperature of the medium during the plant cell culture 有权
    通过在植物细胞培养期间改变培养基的温度来批量生产紫杉醇

    公开(公告)号：US06589765B1

    公开(公告)日：2003-07-08

    申请号：US09269154

    申请日：1999-02-26

    申请人： Ho-Joon Choi ,  Hyung-Kyoon Choi ,  Sang-Ic Kim ,  Jeong-Hwan Yun ,  Joo-Sun Son ,  Moon-Seok Chang ,  Eunsoo Choi ,  Hong-Rak Kim ,  Seung-Suh Hong ,  Hyun-Soo Lee

    发明人： Ho-Joon Choi ,  Hyung-Kyoon Choi ,  Sang-Ic Kim ,  Jeong-Hwan Yun ,  Joo-Sun Son ,  Moon-Seok Chang ,  Eunsoo Choi ,  Hong-Rak Kim ,  Seung-Suh Hong ,  Hyun-Soo Lee

    IPC分类号： C12P1702

    CPC分类号： C12N5/04 ,  C12P17/02

    摘要： The present invention relate to a method to increase the production level of paclitaxel by changing the temperature during the plant cell culture. According to the present invention, the production of the paclitaxel comprises the following procedure: (i) cultivating the Taxus genus plant cells in a medium at ca. 20 to 25° C.; and (ii) when growth of the plant cells has progressed sufficiently, changing the cultivation temperature to ca. 26 to 32° C. to continue the culture. The present invention comprises also the method of increasing the paclitaxel production by inoculating the cells at a high initial concentration and by increasing the saccharide concentration in the medium: According to the present invention, paclitaxel can be mass-produced conveniently and therefore has an industrial application.

    摘要翻译： 本发明涉及通过改变植物细胞培养过程中的温度来提高紫杉醇生产水平的方法。 根据本发明，紫杉醇的制备包括以下步骤：（i）在培养基中培养紫杉属植物细胞。 20至25℃。 和（ii）当植物细胞生长充分进展时，将培养温度改变为约 26至32°C继续培养。 本发明还包括通过以高初始浓度接种细胞并增加培养基中的糖浓度来增加紫杉醇产生的方法：根据本发明，紫杉醇可以方便批量生产，因此具有工业应用 。

    Cell growth inhibiting and cell differentiation specific SYG972 gene, genomic DNA and promoter thereof
    8.
    发明授权
    Cell growth inhibiting and cell differentiation specific SYG972 gene, genomic DNA and promoter thereof 失效
    细胞生长抑制和细胞分化特异性SYG972基因，基因组DNA及其启动子

    公开(公告)号：US06573371B2

    公开(公告)日：2003-06-03

    申请号：US09879833

    申请日：2001-06-12

    申请人： Neon-Cheol Jung ,  Jin-Man Kim ,  Seung-Suh Hong ,  Hyun-Soo Lee ,  Young-Rim Seong ,  Jae-Ho Lee

    发明人： Neon-Cheol Jung ,  Jin-Man Kim ,  Seung-Suh Hong ,  Hyun-Soo Lee ,  Young-Rim Seong ,  Jae-Ho Lee

    IPC分类号： C07H2102

    CPC分类号： C12Q1/6886 ,  C12Q2600/156 ,  C12Q2600/158

    摘要： The present invention relates to a diagnosis method of cancer using SYG972 gene and to the genomic DNA and to a promoter regulating transcription of the gene. Expression of SYG972 gene is greatly reduced in breast cancer cells compared to normal breast cells. Therefore the present invention provides a method of diagnosis method by using the gene of the present invention or its fragments as a probe. SYG972 genomic according to the present invention is composed of 4 exons and 3 introns. Promoter is 3 kb long from the transcription initiating site in the 5′ direction. SYG972 genomic DNA and promoter can be used to design and screen drugs to promote or to inhibit apoptosis and differentiation of cells, especially to screen drugs to treat cancer, a disease wherein cell growth and differentiation is abnormal.

    摘要翻译： 本发明涉及使用SYG972基因和基因组DNA的癌症诊断方法以及调节基因转录的启动子。 与正常乳腺细胞相比，乳腺癌细胞中SYG972基因的表达大大降低。 因此，本发明提供了通过使用本发明的基因或其片段作为探针的诊断方法。 根据本发明的SYG972基因组由4个外显子和3个内含子组成。 启动子距5'方向的转录起始位点长3 kb。 SYG972基因组DNA和启动子可用于设计和筛选药物，以促进或抑制细胞的凋亡和分化，特别是筛选药物以治疗癌症，其中细胞生长和分化异常。

    Method for treating rheumatoid arthritis with composition containing histone
    9.
    发明授权
    Method for treating rheumatoid arthritis with composition containing histone 有权
    用含有组蛋白的组合物治疗类风湿性关节炎的方法

    公开(公告)号：US06204242B1

    公开(公告)日：2001-03-20

    申请号：US09381559

    申请日：2000-01-19

    申请人： Insoo Bae ,  Dong-soo Kim ,  Heajoon Yim ,  Neon-Cheol Jung ,  Yong-Weon Yi ,  Seung-Suh Hong ,  Hyun-Soo Lee

    发明人： Insoo Bae ,  Dong-soo Kim ,  Heajoon Yim ,  Neon-Cheol Jung ,  Yong-Weon Yi ,  Seung-Suh Hong ,  Hyun-Soo Lee

    IPC分类号： A61K3800

    CPC分类号： A61K38/1709

    摘要： The present invention relates to a novel use of histone and provides a pharmaceutical composition containing histone as an active ingredient to improve the symptoms of progressive, inflammatory and autoimmune arthritis. The pharmaceutical composition of the present invention includes histone, especially histone H1 as an active ingredient, and could include pharmacologically approved carriers if necessary. Histone H1 lowered induction of arthritis and reduced arthritis index more effectively than steroidal dexamethasone and also had a significant preventive effect.

    摘要翻译： 本发明涉及组蛋白的新用途，并提供含有组蛋白作为活性成分以改善进行性，炎性和自身免疫性关节炎症状的药物组合物。 本发明的药物组合物包括组蛋白，特别是组蛋白H1作为活性成分，如果需要，可以包括药理学上认可的载体。 组蛋白H1比甾体地塞米松更有效地降低了关节炎的诱导，降低了关节炎指数，并且具有显着的预防作用。