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    Method and reagents for assessing lipoprotein metabolism
    1.
    发明授权
    Method and reagents for assessing lipoprotein metabolism 失效
    评估脂蛋白代谢的方法和试剂

    公开(公告)号：US6074834A

    公开(公告)日：2000-06-13

    申请号：US36454

    申请日：1998-03-06

    申请人： Sue Ellen Slezak ,  John H. Abel ,  Barbara Obrepalska-Bielska ,  Eugene A. Nau ,  Kathy L. Gottlund

    发明人： Sue Ellen Slezak ,  John H. Abel ,  Barbara Obrepalska-Bielska ,  Eugene A. Nau ,  Kathy L. Gottlund

    IPC分类号： G01N33/531 ,  G01N33/92 ,  G01N33/567

    CPC分类号： G01N33/531 ,  G01N33/92 ,  G01N2333/705 ,  G01N2800/044 ,  Y10S435/81

    摘要： The present invention provides methods and reagents for assessing lipoprotein metabolism. The methods provided are applicable for use on multiple samples in a clinical laboratory, thus obviating the need for sophisticated instrumentation, such as flow cytometry. Selected cell types in a test sample are uniformly labelled with a detectable reporter substance, so that they may later be enumerated. Pre-determined lipoprotein receptors associated with the cells of interest are labelled with a receptor-selective marker, thereby to determine the number of lipoprotein receptors per cell in the test sample. Selected cells of interest are conveniently separated from the test sample by binding thereto a specific binding substance attached to a solid support. The specific binding substance binds specifically with a characteristic determinant of the cell subset of interest. The remainder of the test sample may be washed away or discarded. The separated cells of interest may then be enumerated by measuring the amount of detectable label associated therewith, hence to determine the number of lipoprotein receptors per cell in each test sample.

    摘要翻译： 本发明提供了用于评估脂蛋白代谢的方法和试剂。 所提供的方法适用于临床实验室中的多个样品，从而避免需要复杂的仪器，如流式细胞术。 测试样品中选定的细胞类型用可检测的报道物质均匀地标记，以便随后可列举它们。 与感兴趣的细胞相关的预定脂蛋白受体用受体选择性标记物标记，从而确定测试样品中每个细胞的脂蛋白受体数。 所选择的细胞通过与附着在固体支持物上的特异性结合物质结合而方便地与测试样品分离。 特异性结合物质与感兴趣的细胞亚群的特征决定簇特异性结合。 测试样品的其余部分可能被冲走或丢弃。 然后可以通过测量与其相关联的可检测标记的量来列举所分离的感兴趣的细胞，从而确定每个测试样品中每个细胞的脂蛋白受体的数量。