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公开(公告)号:US20090197775A1
公开(公告)日:2009-08-06
申请号:US12247922
申请日:2008-10-08
申请人: Till Bachmann , Timo Barl , Heinz Gerhard Kohn
发明人: Till Bachmann , Timo Barl , Heinz Gerhard Kohn
CPC分类号: C12Q1/6827
摘要: The present invention pertains to a method for determining point mutations on micro-arrays/biochips using a nuclease capable to selectively digest hybridized DNA strands into two DNA fragments, said hybridized DNA strands containing a nucleotide mismatch or nucleotide deletion. The occurrence and/or location of such DNA fragments are indicative for a point mutation.
摘要翻译: 本发明涉及使用能够将杂交的DNA链选择性地将两条DNA片段消化的核酸酶来确定微阵列/生物芯片上的点突变的方法,所述杂交DNA链含有核苷酸错配或核苷酸缺失。 这种DNA片段的出现和/或位置指示点突变。
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公开(公告)号:US20050069901A1
公开(公告)日:2005-03-31
申请号:US10673038
申请日:2003-09-29
申请人: Verena Grimm , Santoshi Ezaki , Milorad Susa , Cornelius Knabe , Rolf Schmid , Till Bachmann
发明人: Verena Grimm , Santoshi Ezaki , Milorad Susa , Cornelius Knabe , Rolf Schmid , Till Bachmann
IPC分类号: C12Q1/68
CPC分类号: C12Q1/689 , C12Q1/6837 , C12Q1/6876 , C12Q2600/156 , C12Q2565/519 , C12Q2525/179
摘要: The present invention provides methods for detecting a micro-organism in a biological sample and simultaneously determining its resistance to antibiotics. The methods utilize micro-arrays to evaluate microbrial genes often associated with antibiotic resistance.
摘要翻译: 本发明提供了用于检测生物样品中微生物并同时测定其对抗生素抗性的方法。 该方法利用微阵列来评估通常与抗生素耐药相关的微生物基因。
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公开(公告)号:US08778157B2
公开(公告)日:2014-07-15
申请号:US12921902
申请日:2009-03-11
申请人: Holger Schulze , Till Bachmann , Andrew Mount
发明人: Holger Schulze , Till Bachmann , Andrew Mount
IPC分类号: G01N27/447 , G01N27/327
CPC分类号: B03C5/026 , B01L3/50273 , B01L2200/0652 , B01L2300/0645 , B01L2400/0424 , B03C5/005 , C12Q1/6816 , G01N33/561 , C12Q2523/307 , C12Q2565/607
摘要: Provided is a method for processing a sample, which method comprises: a) contacting a binding phase, which binding phase is capable of binding an analyte, with the sample in the presence of a medium; b) applying across the medium a first alternating field composed of a plurality of pulses and having a first frequency, a first pulse duration and a first pulse rise time; c) optionally applying across the medium a second alternating field; and d) thereby influencing the sample and/or the binding phase in the medium.
摘要翻译: 提供了一种处理样品的方法,该方法包括:a)在介质存在下使结合相与结合相结合,该结合相能够与分析物结合; b)在介质上施加由多个脉冲组成的具有第一频率,第一脉冲持续时间和第一脉冲上升时间的第一交变场; c)任选地在所述介质上施加第二交变场; 和d)从而影响培养基中的样品和/或结合相。
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公开(公告)号:US20110077166A1
公开(公告)日:2011-03-31
申请号:US12922140
申请日:2009-03-10
申请人: Colin Campbell , Holger Schulze , Till Bachmann
发明人: Colin Campbell , Holger Schulze , Till Bachmann
CPC分类号: C12Q1/6837 , B01J2219/00605 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00722 , B01J2219/00725 , C40B50/18 , C40B70/00 , G01N33/54353
摘要: Provided is a method of attaching a substance to a surface, which method comprises contacting a surface comprising amine reactive groups with a substance labelled with a peptide tag such that the substance is covalently attached to the surface via the peptide tag, wherein the peptide tag comprises one or more histidine residues, one of which is a terminal histidine residue having a free N-terminal amino group. Also provided is a method of processing or analysis which comprises a method of attaching a substance to a surface as detailed above and comprises one or more further steps of processing or analysing the substance. The present invention further provides a method of processing or analysis comprising the steps of: a) attaching a substance to a surface by a method as defined above b) contacting the surface with a sample comprising an analyte in order that the analyte in the sample binds to the substance attached to the surface; and c) processing and/or analysing the analyte
摘要翻译: 提供了将物质附着到表面的方法,该方法包括使包含胺反应性基团的表面与标记有肽标签的物质接触,使得物质通过肽标签共价连接到表面,其中肽标签包含 一个或多个组氨酸残基,其中之一是具有游离N-末端氨基的末端组氨酸残基。 还提供了一种处理或分析方法,其包括将物质附着到如上所述的表面的方法,并且包括处理或分析物质的一个或多个其它步骤。 本发明还提供了一种加工或分析方法,包括以下步骤:a)通过上述方法将物质附着于表面b)使表面与包含分析物的样品接触,以使样品中的分析物结合 附着于表面的物质; 和c)处理和/或分析分析物
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公开(公告)号:US20110031123A1
公开(公告)日:2011-02-10
申请号:US12921902
申请日:2009-03-11
申请人: Holger Schulze , Till Bachmann , Andrew Mount
发明人: Holger Schulze , Till Bachmann , Andrew Mount
IPC分类号: G01N27/26 , G01N27/447
CPC分类号: B03C5/026 , B01L3/50273 , B01L2200/0652 , B01L2300/0645 , B01L2400/0424 , B03C5/005 , C12Q1/6816 , G01N33/561 , C12Q2523/307 , C12Q2565/607
摘要: Provided is a method for processing a sample, which method comprises:a) contacting a binding phase, which binding phase is capable of binding an analyte, with the sample in the presence of a medium;b) applying across the medium a first alternating field composed of a plurality of pulses and having a first frequency, a first pulse duration and a first pulse rise time;c) optionally applying across the medium a second alternating field; andd) thereby influencing the sample and/or the binding phase in the medium.
摘要翻译: 提供了一种处理样品的方法,该方法包括:a)在介质存在下使结合相与结合相结合,该结合相能够与分析物结合; b)在介质上施加由多个脉冲组成的具有第一频率,第一脉冲持续时间和第一脉冲上升时间的第一交变场; c)任选地在所述介质上施加第二交变场; 和d)从而影响培养基中的样品和/或结合相。
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6.
公开(公告)号:US20080096203A1
公开(公告)日:2008-04-24
申请号:US11767145
申请日:2007-06-22
申请人: Xiaolei Yu , Milorad Susa , Cornelius Knabbe , Rolf Schmid , Till Bachmann
发明人: Xiaolei Yu , Milorad Susa , Cornelius Knabbe , Rolf Schmid , Till Bachmann
IPC分类号: C12Q1/68
CPC分类号: C12Q1/689 , C12Q2600/156
摘要: The present invention pertains to a method for detecting quinolone-resistant Escherichia coli strains in a biological sample. The present invention also relates to a kit adapted to perform the inventive method.
摘要翻译: 本发明涉及生物样品中喹诺酮抗性大肠杆菌菌株的检测方法。 本发明还涉及适于执行本发明方法的试剂盒。
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公开(公告)号:US20060216802A1
公开(公告)日:2006-09-28
申请号:US10555719
申请日:2004-05-05
申请人: Bernhard Hauer , Tilo Habicher , Rolf Schmid , Steffen Maurer , Vlada Urlacher , Holger Schulze , Norbert Huber , Till Bachmann
发明人: Bernhard Hauer , Tilo Habicher , Rolf Schmid , Steffen Maurer , Vlada Urlacher , Holger Schulze , Norbert Huber , Till Bachmann
CPC分类号: C12N9/0077
摘要: A process for preparing a hydroxylation catalyst by i) embedding a cystochrome P450 monooxygenase in a sol-gel matrix, ii) embedding an enzymatic NADPH-regenerating system in a sol-gel matrix, and combining the two components i) and ii) unless they were already mixed together before the embedding.
摘要翻译: 一种制备羟基化催化剂的方法,其方法是i)将囊状P450单加氧酶包埋在溶胶 - 凝胶基质中,ii)将酶NADPH再生系统包埋在溶胶 - 凝胶基质中,并将两种组分i)和ii)组合,除非它们 在嵌入之前已经混合在一起。
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公开(公告)号:US07943299B2
公开(公告)日:2011-05-17
申请号:US10408494
申请日:2003-04-07
申请人: Satoshi Ezaki , Stefanie Häfele , Till Bachmann , Rolf D. Schmid
发明人: Satoshi Ezaki , Stefanie Häfele , Till Bachmann , Rolf D. Schmid
IPC分类号: C12Q1/68 , C12M1/34 , C07K14/435
CPC分类号: C12Q1/6827 , C12Q1/6832 , C12Q2522/101
摘要: The present invention relates to an improved method for determining nucleotide sequences or nucleotide sequences on biochips/micro-assays, for example, using p53 mediated hybridization. The present invention relates particularly to the use of p53 polypeptides in determining changes in nucleotide sequences.
摘要翻译: 本发明涉及用于确定生物芯片/微量测定上的核苷酸序列或核苷酸序列的改进方法,例如使用p53介导的杂交。 本发明特别涉及p53多肽在确定核苷酸序列变化中的用途。
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公开(公告)号:US20110100820A1
公开(公告)日:2011-05-05
申请号:US12994424
申请日:2009-03-20
申请人: Till Bachmann , Andrew Mount , Anthony Walton
发明人: Till Bachmann , Andrew Mount , Anthony Walton
IPC分类号: G01N27/447
CPC分类号: B03C5/026 , B01L3/502761 , B01L2200/0647 , B01L2400/0424
摘要: Provided is a device for assaying one or more analytes, said device comprising an electrode, a means for optical detection; and a means for electrochemical detection, wherein the device is configured such that the electrode is capable of promoting transport of an analyte when a field is applied to the analyte via the electrode, and wherein the means for electrochemical detection employs the electrode and the means for optical detection employs the electrode, and wherein the device is configured to carry out dielectrophoresis.Further provided is the use of the device of the present invention for promoting transport of an analyte, detecting the optical properties of the analyte and detecting the electrochemical properties of the analyte.Also provided is method for assaying one or more analytes, which method comprises the steps of: promoting transport of an analyte, performing an optical measurement of the analyte and performing an electrochemical measurement of the analyte, which method employs the device of the present invention.
摘要翻译: 提供了一种用于测定一种或多种分析物的装置,所述装置包括电极,用于光学检测的装置; 以及用于电化学检测的装置,其中所述装置被配置为使得当通过所述电极将场施加到所述分析物时,所述电极能够促进分析物的运输,并且其中所述用于电化学检测的装置使用所述电极和所述装置 光学检测使用电极,并且其中该器件被配置为执行介电电泳。 还提供了本发明的装置用于促进分析物的运输,检测分析物的光学性质和检测分析物的电化学性质的用途。 还提供了用于测定一种或多种分析物的方法,该方法包括以下步骤:促进分析物的转运,进行分析物的光学测量并进行分析物的电化学测量,该方法采用本发明的装置。
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公开(公告)号:US20060210999A1
公开(公告)日:2006-09-21
申请号:US11083788
申请日:2005-03-18
申请人: Verena Grimm , Milorad Susa , Cornelius Knabbe , Rolf Schmid , Till Bachmann
发明人: Verena Grimm , Milorad Susa , Cornelius Knabbe , Rolf Schmid , Till Bachmann
IPC分类号: C12Q1/68
CPC分类号: C12Q1/689 , C12Q2600/156 , C12Q2600/166
摘要: The present invention relates to a method for genotyping SHV beta lactamase and a method for determining antibiotic resistance conferred by a SHV-type Extended Spectrum Beta Lactamase of a bacterium. Moreover, the present invention pertains to an oligonucleotide array and a kit for a genotyping based detection of SHV beta lactamases, as well as to a method for designing improved oligonucleotide capture probes.
摘要翻译: 本发明涉及用于基因分型SHVβ内酰胺酶的方法和由细菌的SHV型扩展光谱β-内酰胺酶赋予的抗生素抗性的方法。 此外,本发明涉及寡核苷酸阵列和用于基于SHVβ内酰胺酶检测的基因分型的试剂盒,以及设计改进的寡核苷酸捕获探针的方法。
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