NUCLEASE ON CHIP
    1.
    发明申请
    NUCLEASE ON CHIP 审中-公开
    芯片上的核

    公开(公告)号:US20090197775A1

    公开(公告)日:2009-08-06

    申请号:US12247922

    申请日:2008-10-08

    IPC分类号: C40B30/04 C12Q1/68

    CPC分类号: C12Q1/6827

    摘要: The present invention pertains to a method for determining point mutations on micro-arrays/biochips using a nuclease capable to selectively digest hybridized DNA strands into two DNA fragments, said hybridized DNA strands containing a nucleotide mismatch or nucleotide deletion. The occurrence and/or location of such DNA fragments are indicative for a point mutation.

    摘要翻译: 本发明涉及使用能够将杂交的DNA链选择性地将两条DNA片段消化的核酸酶来确定微阵列/生物芯片上的点突变的方法,所述杂交DNA链含有核苷酸错配或核苷酸缺失。 这种DNA片段的出现和/或位置指示点突变。

    Detecting analytes
    3.
    发明授权
    Detecting analytes 有权
    检测分析物

    公开(公告)号:US08778157B2

    公开(公告)日:2014-07-15

    申请号:US12921902

    申请日:2009-03-11

    IPC分类号: G01N27/447 G01N27/327

    摘要: Provided is a method for processing a sample, which method comprises: a) contacting a binding phase, which binding phase is capable of binding an analyte, with the sample in the presence of a medium; b) applying across the medium a first alternating field composed of a plurality of pulses and having a first frequency, a first pulse duration and a first pulse rise time; c) optionally applying across the medium a second alternating field; and d) thereby influencing the sample and/or the binding phase in the medium.

    摘要翻译: 提供了一种处理样品的方法,该方法包括:a)在介质存在下使结合相与结合相结合,该结合相能够与分析物结合; b)在介质上施加由多个脉冲组成的具有第一频率,第一脉冲持续时间和第一脉冲上升时间的第一交变场; c)任选地在所述介质上施加第二交变场; 和d)从而影响培养基中的样品和/或结合相。

    SURFACE ATTACHMENT
    4.
    发明申请
    SURFACE ATTACHMENT 审中-公开
    表面贴附

    公开(公告)号:US20110077166A1

    公开(公告)日:2011-03-31

    申请号:US12922140

    申请日:2009-03-10

    摘要: Provided is a method of attaching a substance to a surface, which method comprises contacting a surface comprising amine reactive groups with a substance labelled with a peptide tag such that the substance is covalently attached to the surface via the peptide tag, wherein the peptide tag comprises one or more histidine residues, one of which is a terminal histidine residue having a free N-terminal amino group. Also provided is a method of processing or analysis which comprises a method of attaching a substance to a surface as detailed above and comprises one or more further steps of processing or analysing the substance. The present invention further provides a method of processing or analysis comprising the steps of: a) attaching a substance to a surface by a method as defined above b) contacting the surface with a sample comprising an analyte in order that the analyte in the sample binds to the substance attached to the surface; and c) processing and/or analysing the analyte

    摘要翻译: 提供了将物质附着到表面的方法,该方法包括使包含胺反应性基团的表面与标记有肽标签的物质接触,使得物质通过肽标签共价连接到表面,其中肽标签包含 一个或多个组氨酸残基,其中之一是具有游离N-末端氨基的末端组氨酸残基。 还提供了一种处理或分析方法,其包括将物质附着到如上所述的表面的方法,并且包括处理或分析物质的一个或多个其它步骤。 本发明还提供了一种加工或分析方法,包括以下步骤:a)通过上述方法将物质附着于表面b)使表面与包含分析物的样品接触,以使样品中的分析物结合 附着于表面的物质; 和c)处理和/或分析分析物

    DETECTING ANALYTES
    5.
    发明申请
    DETECTING ANALYTES 有权
    检测分析

    公开(公告)号:US20110031123A1

    公开(公告)日:2011-02-10

    申请号:US12921902

    申请日:2009-03-11

    IPC分类号: G01N27/26 G01N27/447

    摘要: Provided is a method for processing a sample, which method comprises:a) contacting a binding phase, which binding phase is capable of binding an analyte, with the sample in the presence of a medium;b) applying across the medium a first alternating field composed of a plurality of pulses and having a first frequency, a first pulse duration and a first pulse rise time;c) optionally applying across the medium a second alternating field; andd) thereby influencing the sample and/or the binding phase in the medium.

    摘要翻译: 提供了一种处理样品的方法,该方法包括:a)在介质存在下使结合相与结合相结合,该结合相能够与分析物结合; b)在介质上施加由多个脉冲组成的具有第一频率,第一脉冲持续时间和第一脉冲上升时间的第一交变场; c)任选地在所述介质上施加第二交变场; 和d)从而影响培养基中的样品和/或结合相。

    TRIPLE FUNCTION ELECTRODES
    9.
    发明申请
    TRIPLE FUNCTION ELECTRODES 审中-公开
    三功能电极

    公开(公告)号:US20110100820A1

    公开(公告)日:2011-05-05

    申请号:US12994424

    申请日:2009-03-20

    IPC分类号: G01N27/447

    摘要: Provided is a device for assaying one or more analytes, said device comprising an electrode, a means for optical detection; and a means for electrochemical detection, wherein the device is configured such that the electrode is capable of promoting transport of an analyte when a field is applied to the analyte via the electrode, and wherein the means for electrochemical detection employs the electrode and the means for optical detection employs the electrode, and wherein the device is configured to carry out dielectrophoresis.Further provided is the use of the device of the present invention for promoting transport of an analyte, detecting the optical properties of the analyte and detecting the electrochemical properties of the analyte.Also provided is method for assaying one or more analytes, which method comprises the steps of: promoting transport of an analyte, performing an optical measurement of the analyte and performing an electrochemical measurement of the analyte, which method employs the device of the present invention.

    摘要翻译: 提供了一种用于测定一种或多种分析物的装置,所述装置包括电极,用于光学检测的装置; 以及用于电化学检测的装置,其中所述装置被配置为使得当通过所述电极将场施加到所述分析物时,所述电极能够促进分析物的运输,并且其中所述用于电化学检测的装置使用所述电极和所述装置 光学检测使用电极,并且其中该器件被配置为执行介电电泳。 还提供了本发明的装置用于促进分析物的运输,检测分析物的光学性质和检测分析物的电化学性质的用途。 还提供了用于测定一种或多种分析物的方法,该方法包括以下步骤:促进分析物的转运,进行分析物的光学测量并进行分析物的电化学测量,该方法采用本发明的装置。

    Microarray and method for genotyping SHV beta lactamases
    10.
    发明申请
    Microarray and method for genotyping SHV beta lactamases 审中-公开
    微阵列和基因分型SHVβ-内酰胺酶的方法

    公开(公告)号:US20060210999A1

    公开(公告)日:2006-09-21

    申请号:US11083788

    申请日:2005-03-18

    IPC分类号: C12Q1/68

    摘要: The present invention relates to a method for genotyping SHV beta lactamase and a method for determining antibiotic resistance conferred by a SHV-type Extended Spectrum Beta Lactamase of a bacterium. Moreover, the present invention pertains to an oligonucleotide array and a kit for a genotyping based detection of SHV beta lactamases, as well as to a method for designing improved oligonucleotide capture probes.

    摘要翻译: 本发明涉及用于基因分型SHVβ内酰胺酶的方法和由细菌的SHV型扩展光谱β-内酰胺酶赋予的抗生素抗性的方法。 此外,本发明涉及寡核苷酸阵列和用于基于SHVβ内酰胺酶检测的基因分型的试剂盒,以及设计改进的寡核苷酸捕获探针的方法。