公开(公告)号：US20060141474A1

公开(公告)日：2006-06-29

申请号：US10563475

申请日：2004-08-27

申请人： Yuji Miyahara ,  Toshiya Sakata ,  Masao Kamahori ,  Yoshiaki Yazawa

发明人： Yuji Miyahara ,  Toshiya Sakata ,  Masao Kamahori ,  Yoshiaki Yazawa

IPC分类号： C12Q1/68 ,  C12M1/34

CPC分类号： C12Q1/6837 ,  B01J2219/00529 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00653 ,  G01N27/4145 ,  C12Q2565/607 ,  C12Q2563/173

摘要： An inexpensive DNA chip/DNA microarray system capable of highly accurate measurement with low running cost. A DNA probe 8 is immobilized on a floating electrode 7 connected to a gate electrode 5 of a field effect transistor. Hybridization with a target gene is carried out on the surface of the floating electrode, when a change in surface charge density is detected using a field effect.

摘要翻译： 一种廉价的DNA芯片/ DNA微阵列系统，能够以低运行成本进行高精度测量。 DNA探针8被固定在连接到场效应晶体管的栅电极5的浮置电极7上。 当使用场效应检测表面电荷密度的变化时，在浮动电极的表面上进行与靶基因的杂交。

公开(公告)号：US07695907B2

公开(公告)日：2010-04-13

申请号：US10587941

申请日：2005-02-03

申请人： Yuji Miyahara ,  Toshiya Sakata

发明人： Yuji Miyahara ,  Toshiya Sakata

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6825 ,  C12Q2600/156 ,  G01N27/4145 ,  C12Q2565/607 ,  C12Q2565/537

摘要： A gene detection field-effect device provided with an insulation film (2), a semiconductor substrate (3), and a reference electrode (4), includes: (a) the insulation film (2) including a nucleic acid probe (5) immobilized on one of the surfaces thereof and is in contact with a sample solution (6) containing at least one type of a target gene (601) for detection and analysis; (b) the semiconductor substrate (3) being installed so as to abut against the other surface of the insulation film (2); and (c) the reference electrode (4) being provided in the sample solution (6).

摘要翻译： 设置有绝缘膜（2），半导体基板（3）和参考电极（4）的基因检测场效应元件包括：（a）包含核酸探针（5）的绝缘膜（2） 固定在其一个表面上并与含有至少一种类型的目标基因（601）的样品溶液（6）接触以进行检测和分析; （b）半导体衬底（3）被安装成抵靠绝缘膜（2）的另一个表面; 和（c）参考电极（4）设置在样品溶液（6）中。

公开(公告)号：US20110193139A1

公开(公告)日：2011-08-11

申请号：US12852205

申请日：2010-08-06

申请人： Toshiya SAKATA ,  Sayaka KITA

发明人： Toshiya SAKATA ,  Sayaka KITA

IPC分类号： H01L29/772

CPC分类号： G01N27/4145

摘要： There is provided a cell measuring apparatus that can measure cells efficiently. In a cell measuring apparatus 1 provided with an FET sensor 2, an accommodating cavity 21 that can accommodate a cell 15, is provided on a measuring section 20 on which cells immersed in a solution are mounted. The accommodating cavity 21 is formed in a mortar shape. By accommodating a cell 15 in the accommodating cavity 21, the cells 15 can be easily separated, so that measurement can be performed for each cell 15. Therefore the state of the respective cells can be more efficiently measured.

摘要翻译： 提供了可以有效地测量细胞的细胞测量装置。 在设置有FET传感器2的电池测量装置1中，在安装有溶液的电池的测量部20上设置有容纳电池单元15的容纳腔21。 容纳腔21形成为砂浆形状。 通过将电池单元15容纳在容纳腔21中，可以容易地分离电池单元15，从而可以对每个电池单元15进行测量。因此，可以更有效地测量各电池单元的状态。

公开(公告)号：US07888013B2

公开(公告)日：2011-02-15

申请号：US11574353

申请日：2005-08-26

申请人： Yuji Miyahara ,  Toshiya Sakata

发明人： Yuji Miyahara ,  Toshiya Sakata

IPC分类号： C12Q1/68 ,  C07H21/02 ,  C07H21/04

CPC分类号： B01L7/00 ,  B01L3/5027 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0877 ,  G01N27/4145

摘要： Since conventional DNA sequence analyzing technologies are based on the fundamental principle of fluorescent detection, expensive, complex optical systems and laser sources have been necessary.A field-effect device for gene detection of the present invention analyzes a base sequence by immobilizing a single-strand nucleic acid probe at a gate portion, inducing hybridization at the gate portion to form a double-stranded DNA, inducing elongation reaction by adding a DNA polymerase and one of the substrates, and measuring the electrical characteristic of the field-effect device caused by elongation reaction.Since the elongation reaction of one base induced at the gate portion can be directly converted to an electrical signal, expensive lasers or complex optical systems are not needed. Thus, a small gene polymorphism detection system that can conduct measurement at high precision can be provided.

摘要翻译： 由于常规DNA序列分析技术是基于荧光检测的基本原理，因此必须使用昂贵的复杂光学系统和激光源。 本发明的用于基因检测的场效应装置通过在门部固定单链核酸探针来分析碱基序列，在门部诱导杂交以形成双链DNA，通过加入 DNA聚合酶和其中一种底物，并测量由伸长反应引起的场效应装置的电特性。 由于在栅极部分引起的一个基极的伸长反应可以直接转换成电信号，所以不需要昂贵的激光器或复杂的光学系统。 因此，可以提供可以高精度地进行测量的小型基因多态性检测系统。

公开(公告)号：US20100197001A1

公开(公告)日：2010-08-05

申请号：US12696342

申请日：2010-01-29

申请人： Yuji Miyahara ,  Toshiya Sakata

发明人： Yuji Miyahara ,  Toshiya Sakata

IPC分类号： C12M1/00

CPC分类号： C12Q1/6825 ,  C12Q2600/156 ,  G01N27/4145 ,  C12Q2565/607 ,  C12Q2565/537

摘要： A gene detection field-effect device provided with an insulation film (2), a semiconductor substrate (3), and a reference electrode (4), includes: (a) the insulation film (2) including a nucleic acid probe (5) immobilized on one of the surfaces thereof and is in contact with a sample solution (6) containing at least one type of a target gene (601) for detection and analysis; (b) the semiconductor substrate (3) being installed so as to abut against the other surface of the insulation film (2); and (c) the reference electrode (4) being provided in the sample solution (6).

摘要翻译： 设置有绝缘膜（2），半导体基板（3）和参考电极（4）的基因检测场效应元件包括：（a）包含核酸探针（5）的绝缘膜（2） 固定在其一个表面上并与含有至少一种类型的目标基因（601）的样品溶液（6）接触以进行检测和分析; （b）半导体衬底（3）被安装成抵靠绝缘膜（2）的另一个表面; 和（c）参考电极（4）设置在样品溶液（6）中。

公开(公告)号：US20080286767A1

公开(公告)日：2008-11-20

申请号：US11574353

申请日：2005-08-26

申请人： Yuji Miyahara ,  Toshiya Sakata

发明人： Yuji Miyahara ,  Toshiya Sakata

IPC分类号： C12Q1/68 ,  C12M1/40

CPC分类号： B01L7/00 ,  B01L3/5027 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0877 ,  G01N27/4145

摘要： Since conventional DNA sequence analyzing technologies are based on the fundamental principle of fluorescent detection, expensive, complex optical systems and laser sources have been necessary.A field-effect device for gene detection of the present invention analyzes a base sequence by immobilizing a single-strand nucleic acid probe at a gate portion, inducing hybridization at the gate portion to form a double-stranded DNA, inducing elongation reaction by adding a DNA polymerase and one of the substrates, and measuring the electrical characteristic of the field-effect device caused by elongation reaction.Since the elongation reaction of one base induced at the gate portion can be directly converted to an electrical signal, expensive lasers or complex optical systems are not needed. Thus, a small gene polymorphism detection system that can conduct measurement at high precision can be provided.

摘要翻译： 由于常规DNA序列分析技术是基于荧光检测的基本原理，因此必须使用昂贵的复杂光学系统和激光源。 本发明的用于基因检测的场效应装置通过在门部固定单链核酸探针来分析碱基序列，在门部诱导杂交以形成双链DNA，通过加入 DNA聚合酶和其中一种底物，并测量由伸长反应引起的场效应装置的电特性。 由于在栅极部分引起的一个基极的伸长反应可以直接转换为电信号，所以不需要昂贵的激光器或复杂的光学系统。 因此，可以提供可以高精度地进行测量的小型基因多态性检测系统。

公开(公告)号：US20080286762A1

公开(公告)日：2008-11-20

申请号：US10587941

申请日：2005-02-03

申请人： Yuji Miyahara ,  Toshiya Sakata

发明人： Yuji Miyahara ,  Toshiya Sakata

IPC分类号： C12Q1/68 ,  C12M1/00

CPC分类号： C12Q1/6825 ,  C12Q2600/156 ,  G01N27/4145 ,  C12Q2565/607 ,  C12Q2565/537

摘要： A gene detection field-effect device provided with an insulation film (2), a semiconductor substrate (3), and a reference electrode (4), includes:(a) the insulation film (2) including a nucleic acid probe (5) immobilized on one of the surfaces thereof and is in contact with a sample solution (6) containing at least one type of a target gene (601) for detection and analysis; (b) the semiconductor substrate (3) being installed so as to abut against the other surface of the insulation film (2); and (c) the reference electrode (4) being provided in the sample solution (6).

摘要翻译： 设置有绝缘膜（2），半导体基板（3）和参考电极（4）的基因检测场效应元件包括：（a）包含核酸探针（5）的绝缘膜（2） 固定在其一个表面上并与含有至少一种类型的目标基因（601）的样品溶液（6）接触以进行检测和分析; （b）半导体衬底（3）被安装成抵靠绝缘膜（2）的另一个表面; 和（c）参考电极（4）设在样品溶液（6）中。