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    Phenylboronic Acid Monomer and Phenylboronic Acid Polymer
    1.
    发明申请
    Phenylboronic Acid Monomer and Phenylboronic Acid Polymer 审中-公开
    苯硼酸单体和苯硼酸聚合物

    公开(公告)号：US20120283403A1

    公开(公告)日：2012-11-08

    申请号：US13520710

    申请日：2010-12-27

    申请人： Akira Matsumoto ,  Takehiko Ishii ,  Kazunori Kataoka ,  Yuji Miyahara

    发明人： Akira Matsumoto ,  Takehiko Ishii ,  Kazunori Kataoka ,  Yuji Miyahara

    IPC分类号： C07F5/02 ,  C08F230/06 ,  C08F30/06

    CPC分类号： C07F5/025 ,  C08F220/56

    摘要： Disclosed are a phenylboronic acid monomer and a phenylboronic acid polymer, each of which can have a pKa value suitable for the use under physiological environments and can be used for various applications. The pheanylboronic acid monomer has a structure represented by formula (13) and therefore has high hydrophilicity, can have a satisfactorily low pKa value when the phenyl ring is fluorinated, and can have a polymerizable unsaturated bond. The phenylboronic acid monomer has high hydrophilicity at a pKa value of 7.4 or less which is a physiological level, and can be polymerized with a wide variety of monomers to produce polymers suitable for the intended purposes.

    摘要翻译： 公开了苯基硼酸单体和苯基硼酸聚合物，其各自可以具有适合在生理环境下使用的pKa值，并可用于各种应用。 苯基苯基硼酸单体具有式（13）所示的结构，因此具有高亲水性，当苯基环被氟化时，可以具有令人满意的低pKa值，并且可以具有可聚合的不饱和键。 苯基硼酸单体在pKa值为7.4或更低的生物水平下具有高亲水性，并且可以与各种单体聚合以产生适合于预期目的的聚合物。

    Method of analyzing DNA sequence using field-effect device, and base sequence analyzer
    3.
    发明授权
    Method of analyzing DNA sequence using field-effect device, and base sequence analyzer 有权
    使用场效应分析DNA序列的方法和碱基序列分析仪

    公开(公告)号：US07888013B2

    公开(公告)日：2011-02-15

    申请号：US11574353

    申请日：2005-08-26

    申请人： Yuji Miyahara ,  Toshiya Sakata

    发明人： Yuji Miyahara ,  Toshiya Sakata

    IPC分类号： C12Q1/68 ,  C07H21/02 ,  C07H21/04

    CPC分类号： B01L7/00 ,  B01L3/5027 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0877 ,  G01N27/4145

    摘要： Since conventional DNA sequence analyzing technologies are based on the fundamental principle of fluorescent detection, expensive, complex optical systems and laser sources have been necessary.A field-effect device for gene detection of the present invention analyzes a base sequence by immobilizing a single-strand nucleic acid probe at a gate portion, inducing hybridization at the gate portion to form a double-stranded DNA, inducing elongation reaction by adding a DNA polymerase and one of the substrates, and measuring the electrical characteristic of the field-effect device caused by elongation reaction.Since the elongation reaction of one base induced at the gate portion can be directly converted to an electrical signal, expensive lasers or complex optical systems are not needed. Thus, a small gene polymorphism detection system that can conduct measurement at high precision can be provided.

    摘要翻译： 由于常规DNA序列分析技术是基于荧光检测的基本原理，因此必须使用昂贵的复杂光学系统和激光源。 本发明的用于基因检测的场效应装置通过在门部固定单链核酸探针来分析碱基序列，在门部诱导杂交以形成双链DNA，通过加入 DNA聚合酶和其中一种底物，并测量由伸长反应引起的场效应装置的电特性。 由于在栅极部分引起的一个基极的伸长反应可以直接转换成电信号，所以不需要昂贵的激光器或复杂的光学系统。 因此，可以提供可以高精度地进行测量的小型基因多态性检测系统。

    GENE DETECTION FIELD-EFFECT DEVICE AND METHOD OF ANALYZING GENE POLYMORPHISM THEREWITH
    4.
    发明申请
    GENE DETECTION FIELD-EFFECT DEVICE AND METHOD OF ANALYZING GENE POLYMORPHISM THEREWITH 审中-公开
    基因检测场效应装置及其分析基因多态性的方法

    公开(公告)号：US20100197001A1

    公开(公告)日：2010-08-05

    申请号：US12696342

    申请日：2010-01-29

    申请人： Yuji Miyahara ,  Toshiya Sakata

    发明人： Yuji Miyahara ,  Toshiya Sakata

    IPC分类号： C12M1/00

    CPC分类号： C12Q1/6825 ,  C12Q2600/156 ,  G01N27/4145 ,  C12Q2565/607 ,  C12Q2565/537

    摘要： A gene detection field-effect device provided with an insulation film (2), a semiconductor substrate (3), and a reference electrode (4), includes: (a) the insulation film (2) including a nucleic acid probe (5) immobilized on one of the surfaces thereof and is in contact with a sample solution (6) containing at least one type of a target gene (601) for detection and analysis; (b) the semiconductor substrate (3) being installed so as to abut against the other surface of the insulation film (2); and (c) the reference electrode (4) being provided in the sample solution (6).

    摘要翻译： 设置有绝缘膜（2），半导体基板（3）和参考电极（4）的基因检测场效应元件包括：（a）包含核酸探针（5）的绝缘膜（2） 固定在其一个表面上并与含有至少一种类型的目标基因（601）的样品溶液（6）接触以进行检测和分析; （b）半导体衬底（3）被安装成抵靠绝缘膜（2）的另一个表面; 和（c）参考电极（4）设置在样品溶液（6）中。

    Method of Analyzing Dna Sequence Using Field-Effect Device, and Base Sequence Analyzer
    5.
    发明申请
    Method of Analyzing Dna Sequence Using Field-Effect Device, and Base Sequence Analyzer 有权
    使用场效应器件分析Dna序列的方法和基本序列分析仪

    公开(公告)号：US20080286767A1

    公开(公告)日：2008-11-20

    申请号：US11574353

    申请日：2005-08-26

    申请人： Yuji Miyahara ,  Toshiya Sakata

    发明人： Yuji Miyahara ,  Toshiya Sakata

    IPC分类号： C12Q1/68 ,  C12M1/40

    CPC分类号： B01L7/00 ,  B01L3/5027 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0877 ,  G01N27/4145

    摘要： Since conventional DNA sequence analyzing technologies are based on the fundamental principle of fluorescent detection, expensive, complex optical systems and laser sources have been necessary.A field-effect device for gene detection of the present invention analyzes a base sequence by immobilizing a single-strand nucleic acid probe at a gate portion, inducing hybridization at the gate portion to form a double-stranded DNA, inducing elongation reaction by adding a DNA polymerase and one of the substrates, and measuring the electrical characteristic of the field-effect device caused by elongation reaction.Since the elongation reaction of one base induced at the gate portion can be directly converted to an electrical signal, expensive lasers or complex optical systems are not needed. Thus, a small gene polymorphism detection system that can conduct measurement at high precision can be provided.

    摘要翻译： 由于常规DNA序列分析技术是基于荧光检测的基本原理，因此必须使用昂贵的复杂光学系统和激光源。 本发明的用于基因检测的场效应装置通过在门部固定单链核酸探针来分析碱基序列，在门部诱导杂交以形成双链DNA，通过加入 DNA聚合酶和其中一种底物，并测量由伸长反应引起的场效应装置的电特性。 由于在栅极部分引起的一个基极的伸长反应可以直接转换为电信号，所以不需要昂贵的激光器或复杂的光学系统。 因此，可以提供可以高精度地进行测量的小型基因多态性检测系统。

    Gene Detection Field-Effect Device And Method Of Analyzing Gene Polymorphism Therewith
    6.
    发明申请
    Gene Detection Field-Effect Device And Method Of Analyzing Gene Polymorphism Therewith 有权
    基因检测场效应装置及其分析基因多态性的方法

    公开(公告)号：US20080286762A1

    公开(公告)日：2008-11-20

    申请号：US10587941

    申请日：2005-02-03

    申请人： Yuji Miyahara ,  Toshiya Sakata

    发明人： Yuji Miyahara ,  Toshiya Sakata

    IPC分类号： C12Q1/68 ,  C12M1/00

    CPC分类号： C12Q1/6825 ,  C12Q2600/156 ,  G01N27/4145 ,  C12Q2565/607 ,  C12Q2565/537

    摘要： A gene detection field-effect device provided with an insulation film (2), a semiconductor substrate (3), and a reference electrode (4), includes:(a) the insulation film (2) including a nucleic acid probe (5) immobilized on one of the surfaces thereof and is in contact with a sample solution (6) containing at least one type of a target gene (601) for detection and analysis; (b) the semiconductor substrate (3) being installed so as to abut against the other surface of the insulation film (2); and (c) the reference electrode (4) being provided in the sample solution (6).

    摘要翻译： 设置有绝缘膜（2），半导体基板（3）和参考电极（4）的基因检测场效应元件包括：（a）包含核酸探针（5）的绝缘膜（2） 固定在其一个表面上并与含有至少一种类型的目标基因（601）的样品溶液（6）接触以进行检测和分析; （b）半导体衬底（3）被安装成抵靠绝缘膜（2）的另一个表面; 和（c）参考电极（4）设在样品溶液（6）中。

    Apparatus for purifying nucleic acid and method of purifying nucleic acid
    8.
    发明授权
    Apparatus for purifying nucleic acid and method of purifying nucleic acid 有权
    核酸纯化装置及核酸纯化方法

    公开(公告)号：US07273702B2

    公开(公告)日：2007-09-25

    申请号：US10478096

    申请日：2002-05-24

    申请人： Teruhisa Akashi ,  Yukiko Ikeda ,  Yoshihiro Nagaoka ,  Naruo Watanabe ,  Yuji Miyahara

    发明人： Teruhisa Akashi ,  Yukiko Ikeda ,  Yoshihiro Nagaoka ,  Naruo Watanabe ,  Yuji Miyahara

    IPC分类号： C12Q1/68 ,  C12M1/00 ,  C12M3/00 ,  C07H21/02

    CPC分类号： B01L3/50273 ,  B01L3/5025 ,  B01L3/502738 ,  B01L3/502753 ,  B01L2200/027 ,  B01L2300/0803 ,  B01L2300/0867 ,  B01L2300/1827 ,  B01L2400/0409 ,  B01L2400/0694 ,  G01N35/00069 ,  G01N2030/381

    摘要： A nucleic acid refining apparatus, being ease in automation thereof, keeping high in contacting frequency between nucleic acid within a sample and a solid phase during nucleic acid capture processing, thereby proving high capturing rate, comprises: means for separating a liquid containing the nucleic acid therein from said sample through centrifugal force; means for transferring a reagent through the centrifugal force; means for producing a mixture liquid of said reagent transferred through the centrifugal force and a solution containing said nucleic acid therein; a carrier for capturing said nucleic acid; means for transferring said mixture liquid to said carrier through the centrifugal force; heating means for heating said carrier; and a holding means for separating and holding the reagent containing said nucleic acid eluting from said carrier, separating from other reagent, through different centrifugal.

    摘要翻译： 一种核酸精制装置，其易于自动化，在核酸捕获处理期间保持样品内的核酸与固相之间的接触频率高，从而证明高捕获率，包括：用于分离含有核酸的液体的装置 其中从所述样品通过离心力; 用于通过离心力转移试剂的装置; 用于生产通过离心力转移的所述试剂的混合液体的装置和其中含有所述核酸的溶液; 用于捕获所述核酸的载体; 用于通过离心力将所述混合液转移到所述载体的装置; 用于加热所述载体的加热装置; 以及用于分离并保持含有从所述载体洗脱的所述核酸的试剂的保持装置，通过不同的离心与其它试剂分离。