公开(公告)号：US20100043882A1

公开(公告)日：2010-02-25

申请号：US12308434

申请日：2007-06-15

申请人： Setsuya Sato ,  Toshiyuki Yamato ,  Katsuaki Hayashi ,  Yoshitaka Matsumoto ,  Takahiro Ochiya ,  Isamu Oh

发明人： Setsuya Sato ,  Toshiyuki Yamato ,  Katsuaki Hayashi ,  Yoshitaka Matsumoto ,  Takahiro Ochiya ,  Isamu Oh

IPC分类号： F16L41/00

CPC分类号： C12M33/00 ,  B01L3/502761 ,  G01N15/1056 ,  Y10T137/0318 ,  Y10T137/85938

摘要： [Problems]To provide a microchip for cell arranging which transfers and arranges the cell accurately and quickly, by combining advantages of the method of an optical tweezer and of micro channel.[Means for Solving Problems]A microchip for arranging cells, including a base, the base comprising: at least one cell supply channel including a cell inlet port in liquid communication with a supply mechanism configured to supply the cells into the cell supply channel; at least one cell drain channel including a cell drain port in fluid communication with a drain mechanism, the cell drain channel configured to guide the cells to the cell drain port; at least one reservoir for temporarily storing the cells flowing between the cell supply channel and the cell drain channel; at least one cell arrangement portion for receiving cells introduced from the reservoir, the cell arrangement portion comprising plural compartments partitioned by a wall; and at least one junction channel extending from the cell arrangement portion to the reservoir; wherein the reservoir is wider than the cell supply channel, the cell drain channel and the junction channel.

摘要翻译： [问题]通过组合光镊方法和微通道的优点，提供一种用于细胞排列的微芯片，其通过精确而快速地传送和排列细胞。 解决问题的手段一种用于布置电池的微芯片，包括基座，所述基座包括：至少一个电池供应通道，包括与供配给将电池供应到电池供应通道的供电机构液体连通的电池入口; 至少一个单元排放通道，包括与漏极机构流体连通的单元排出端口，所述单元排放通道被配置为将单元引导到单元排放端口; 至少一个存储器，用于临时存储在单元供应通道和单元排放通道之间流动的单元; 至少一个用于接收从储存器引入的单元的单元布置部分，单元布置部分包括由壁分隔的多个隔室; 以及从单元布置部分延伸到储存器的至少一个接合通道; 其中所述储存器比所述电池供给通道，所述电池排出通道和所述接合通道宽。

公开(公告)号：US20050276456A1

公开(公告)日：2005-12-15

申请号：US11139015

申请日：2005-05-26

申请人： Toshiyuki Yamato ,  Yoshitaka Matsumoto ,  Katsuaki Hayashi ,  Isamu Oh ,  Setsuya Sato

发明人： Toshiyuki Yamato ,  Yoshitaka Matsumoto ,  Katsuaki Hayashi ,  Isamu Oh ,  Setsuya Sato

IPC分类号： C12M1/34 ,  G02B21/36 ,  G06K9/00

CPC分类号： G06K9/00127 ,  C12M41/36 ,  C12M47/04 ,  G02B21/365

摘要： The present invention provides a cell-operating device comprising a laser light source and a microscope to observe cell sample fluorescently labeled and a camera to take the images of the cell sample that is observed with said microscope and a computer to capture the digital image data obtained by said camera and a group of mirrors comprising electronic control mirrors of which optical axis can be adjusted so that the laser light irradiated from said laser light source can be guided in to said microscope. The computer includes a binarization program for binarizing said digital image data by identifying whether each pixel of said digital image data captures a part of said cell sample according to the fluorescence luminance of each pixel. The computer has an identification program for identifying the boundaries between adjacent different values in the binarized digital image and joins the boundaries into an isolated closed curve, and a data processing program for processing said binarized digital data so that in all the area within said isolated closed curve a cell exists, and a calculation program to divide said binarized digital image data, after running said data processing program, into sections with a predefined size and identify the center of gravity coordinate of each section, and an adjustment program to adjust the optical axis of said electronic control mirrors so that said laser irradiates said center of gravity coordinate. Thus, the present invention is a cell-operating device that the laser light from said laser light source irradiates said center of gravity coordinate.

摘要翻译： 本发明提供了一种细胞操作装置，其包括激光光源和显微镜以观察荧光标记的细胞样品，以及相机拍摄用所述显微镜观察的细胞样品的图像和计算机，以捕获获得的数字图像数据 通过所述相机和一组镜子，包括可以调节光轴的电子控制反射镜，使得可以将从所述激光源照射的激光引导到所述显微镜。 计算机包括二进制化程序，用于通过识别所述数字图像数据的每个像素是否根据每个像素的荧光亮度捕获所述细胞样本的一部分来二值化所述数字图像数据。 计算机具有用于识别二进制数字图像中的相邻不同值之间的边界的识别程序，并将边界连接成隔离闭合曲线，以及用于处理所述二进制数字数据的数据处理程序，使得在所述隔离闭合的所有区域内 曲线单元存在，以及计算程序，用于在运行所述数据处理程序之后将所述二进制数字图像数据划分成具有预定尺寸的区段，并且识别每个区段的重心坐标，以及调整程序以调整光轴 的所述电子控制反射镜，使得所述激光器照射所述重心坐标。 因此，本发明是一种电池操作装置，来自所述激光光源的激光照射所述重心坐标。

公开(公告)号：US20100066880A1

公开(公告)日：2010-03-18

申请号：US12308442

申请日：2007-06-15

申请人： Setsuya Sato ,  Toshiyuki Yamato ,  Katsuaki Hayashi ,  Yoshitaka Matsumoto ,  Isamu Oh

发明人： Setsuya Sato ,  Toshiyuki Yamato ,  Katsuaki Hayashi ,  Yoshitaka Matsumoto ,  Isamu Oh

IPC分类号： H04N5/335 ,  H04N5/225

CPC分类号： G01N15/1484 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2300/0864 ,  B01L2400/0454 ,  B01L2400/0487 ,  C12M47/04 ,  G01N15/1459 ,  G01N15/1463 ,  G01N2015/149

摘要： [Problems] To provide a microchip for cell sorting to obtain the desired cell from a number of cells quickly and accurately by combining the advantages from both methods using the optical tweezer and the liquid supply to the micro channel.[Means for solving problems] A microchip for sorting at least one cell including a base, the base comprising: a cell supply channel for supplying and flowing a liquid containing cells; a cell supply channel including an inlet port in liquid communication with a supply mechanism and a drain port in liquid communication with a drain mechanism; a cell sorting channel intersecting with the cell supply channel at their middle point, the cell sorting channel including an inlet port in liquid communication with a supply mechanism and a drain port in liquid communication with a drain mechanism.

摘要翻译： [问题]提供一种用于细胞分选的微芯片，通过结合使用光学镊子的两种方法和向微通道的液体供应的优点，快速而准确地从许多细胞获得所需的细胞。 解决问题的手段一种用于对至少一个包括基底的电池进行分选的微型芯片，所述基底包括：用于供给和流动含有细胞的液体的电池供给通道; 电池供应通道，其包括与供给机构液体连通的入口和与排出机构液体连通的排出口; 细胞分选通道在其中点处与细胞供应通道相交，细胞分选通道包括与供给机构液体连通的入口和与排出机构液体连通的排出口。