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    Clone-array pooled shotgun strategy for nucleic acid sequencing
    1.
    发明授权
    Clone-array pooled shotgun strategy for nucleic acid sequencing 失效
    用于核酸测序的克隆阵列合并霰弹枪策略

    公开(公告)号：US06975943B2

    公开(公告)日：2005-12-13

    申请号：US09969111

    申请日：2001-09-24

    申请人： Richard Gibbs ,  Allan Bradley ,  Wei-Wen Cai

    发明人： Richard Gibbs ,  Allan Bradley ,  Wei-Wen Cai

    IPC分类号： C12N15/10 ,  C12Q1/68 ,  G06F19/00 ,  G01N33/48

    CPC分类号： C12N15/1034 ,  C12Q1/6874

    摘要： A simplified strategy for sequencing large genomes has been developed. Clone-Array Pooled Shotgun Sequencing (CAPSS) is based upon pooling rows and columns of arrayed genomic clones, for shotgun library construction. Random sequences are accumulated and the data are assembled by sequential comparison of rows and columns, to resolve the sequence of clones at points of intersection. Compared to either a clone-by-clone approach or whole genome shotgun sequencing, CAPSS requires relatively few library constructions and only minimal computational power for a complete genome assembly. The strategy is suitable for sequencing large genomes for which there are no sequence-ready maps, but for which relatively high resolution STS maps and highly redundant BAC libraries are available. It is immediately applicable to the sequencing of mouse, rat, zebra fish and other important genomes, and can be managed in a cooperative fashion to take advantage of the distributed international DNA sequencing capacity.

    摘要翻译： 已经开发了用于测序大基因组的简化策略。 克隆阵列池式霰弹枪测序（CAPSS）基于汇集排列的基因组克隆的行和列，用于霰弹枪库构建。 随机序列被累积，并且数据通过行和列的顺序比较来组装，以解决交叉点处的克隆序列。 与克隆克隆方法或全基因组鸟枪法测序相比，CAPSS需要相对较少的文库构建，并且对于完整的基因组装配仅需要最小的计算能力。 该策略适用于对没有序列准备图的大型基因组进行排序，但对于相对较高分辨率的STS图和高度冗余的BAC库可用。 它可以立即适用于小鼠，大鼠，斑马鱼等重要基因组的测序，可以以合作的方式管理，以利用分布式国际DNA测序能力。

    METHOD FOR SPECIFIC ENRICHMENT OF NUCLEIC ACID SEQUENCES
    2.
    发明申请
    METHOD FOR SPECIFIC ENRICHMENT OF NUCLEIC ACID SEQUENCES 审中-公开
    用于特异性增强核酸序列的方法

    公开(公告)号：US20130130917A1

    公开(公告)日：2013-05-23

    申请号：US13349718

    申请日：2012-01-13

    申请人： Wei-Wen Cai

    发明人： Wei-Wen Cai

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6874 ,  B01J19/0046 ,  B01J2219/00288 ,  B01J2219/00423 ,  B01J2219/00509 ,  C12N15/1003

    摘要： The invention discloses a method for immobilizing nucleic acid probes to solid substrates. Also disclosed is a micro column format for specific sequence capture which enables efficient and convenient enrichment of target sequences from a complex source. The capture probes are immobilized onto microspheres or fibrous filter as the active component inside the column. The column format allows hybridization, post-hybridization wash and recovery of captured sequences all to take place in a simple device without sophisticated equipment.

    摘要翻译： 本发明公开了一种将核酸探针固定在固体基质上的方法。 还公开了用于特定序列捕获的微柱形式，其能够从复杂源有效且方便地富集靶序列。 将捕获探针固定在微球或纤维过滤器上作为柱内活性成分。 列格式允许杂交，杂交后洗涤和捕获序列的恢复全部发生在没有精密设备的简单设备中。

    METHOD FOR PARALLEL AMPLIFICATION OF NUCLEIC ACIDS
    8.
    发明申请
    METHOD FOR PARALLEL AMPLIFICATION OF NUCLEIC ACIDS 审中-公开
    核酸平行放大的方法

    公开(公告)号：US20130005591A1

    公开(公告)日：2013-01-03

    申请号：US13349780

    申请日：2012-01-13

    申请人： Wei-Wen Cai

    发明人： Wei-Wen Cai

    IPC分类号： C40B50/06 ,  C40B30/04 ,  C12P19/34

    CPC分类号： C12Q1/686 ,  C12Q2537/143 ,  C12Q2563/155 ,  C12Q2563/159

    摘要： A new method that enables parallel amplification of nucleic sequences from a complex source is disclosed. The amplification is compartmentized into microdroplets by porous-walled hollow glass microspheres and subjected to PCR thermal cycling or isothermal amplification. The rigid wall of the glass microspheres allows very simple and conventional manipulation of the amplification products for downstream application such as sequencing or detection of copy number of specific sequences in a complex sample.

    摘要翻译： 公开了一种能够从复杂源平行扩增核酸序列的新方法。 通过多孔壁中空玻璃微球将扩增室分成微滴，进行PCR热循环或等温扩增。 玻璃微球的刚性壁允许用于下游应用的扩增产物的非常简单和常规的操作，例如在复杂样品中测序或检测特异性序列的拷贝数。