公开(公告)号：US20140008335A1

公开(公告)日：2014-01-09

申请号：US14003319

申请日：2012-03-11

申请人： Y. Lawrence Yao ,  Gen Satoh

发明人： Y. Lawrence Yao ,  Gen Satoh

IPC分类号： B23K26/32

CPC分类号： B23K26/323 ,  B23K1/0008 ,  B23K1/0056 ,  B23K1/19 ,  B23K26/0622 ,  B23K26/0732 ,  B23K26/26 ,  B23K2101/185 ,  B23K2101/32 ,  B23K2103/24

摘要： A method, article of manufacture, and system for joining different materials is described. In a principal embodiment, two articles, for example different metals, are placed in proximity to one another with an interfacial area. One article is heated using a laser that is scanned across from a point remote from the interface to a point at or just short of the interface. In embodiments, the interfacial bond region is characterized by a homogenous mixed phase region with very low to substantially no brittle intermetallics.

摘要翻译： 描述了用于连接不同材料的方法，制品和系统。 在一个主要实施例中，两个物品，例如不同的金属，被放置在彼此接近的界面面积。 一个文章使用激光进行加热，该激光从远离接口的点扫描到接口处或刚好不到界面的点。 在实施方案中，界面键区域的特征在于具有非常低至基本上不脆的金属间化合物的均匀混合相区域。

公开(公告)号：US20090054222A1

公开(公告)日：2009-02-26

申请号：US11895485

申请日：2007-08-24

申请人： Jian Zhang ,  Alessio Salerno ,  Nabil Simaan ,  Y. Lawrence Yao ,  Gerhard Randers-Pehrson ,  Guy Garty ,  Aparajita Dutta ,  David J. Brenner

发明人： Jian Zhang ,  Alessio Salerno ,  Nabil Simaan ,  Y. Lawrence Yao ,  Gerhard Randers-Pehrson ,  Guy Garty ,  Aparajita Dutta ,  David J. Brenner

IPC分类号： B04B5/10

CPC分类号： C40B30/10 ,  C40B60/12

摘要： Systems and methods for robotic transport are disclosed herein. In some embodiments, robotic systems for transporting biological samples include: a plurality of capillary vessels, in which each capillary vessel can contain a biological sample from a population; a receptacle that can contain the plurality of capillary vessels; a centrifuge; a first robotic device that can transport the receptacle between an input module and the centrifuge; a second robotic device that can transport the receptacle between the centrifuge and a sample harvest location; a cutting device that can cut each of the plurality of capillary vessels; a multi-well plate having a plurality of wells arranged in an array; and a third robotic device that can transfer at least one portion of each of the plurality of biological samples from each of the plurality of capillary vessels to a corresponding well in the array.

摘要翻译： 本文公开了用于机器人运输的系统和方法。 在一些实施例中，用于输送生物样品的机器人系统包括：多个毛细血管，其中每个毛细血管可以包含来自群体的生物样品; 可容纳多个毛细血管的容器; 离心机 能够在输入模块和离心机之间输送容器的第一机器人装置; 第二机器人装置，其可以在离心机和样品收集位置之间运输容器; 切割装置，其可以切割所述多个毛细血管中的每一个; 具有排列成阵列的多个孔的多孔板; 以及第三机器人装置，其可以将多个生物样品中的每一个的至少一部分从多个毛细血管中的每一个转移到阵列中的对应的孔中。

公开(公告)号：US20080179301A1

公开(公告)日：2008-07-31

申请号：US11895517

申请日：2007-08-24

申请人： Guy Garty ,  Gerhard Randers-Pehrson ,  Anubha Bhatla ,  Alessio Salerno ,  Nabil Simaan ,  Y. Lawrence Yao ,  David J. Brenner

发明人： Guy Garty ,  Gerhard Randers-Pehrson ,  Anubha Bhatla ,  Alessio Salerno ,  Nabil Simaan ,  Y. Lawrence Yao ,  David J. Brenner

IPC分类号： B23K26/00 ,  G06K7/10

CPC分类号： C40B30/10 ,  C40B60/12

摘要： Systems and methods for etching materials are disclosed herein. Embodiments of the disclosed subject matter include methods for marking at least one capillary, including etching the at least one capillary with a laser, as well as methods for reading the resulting markings using a second laser. Further embodiments incorporate the second laser within a barcode reader. Various embodiments of the disclosed subject matter include capillaries having outer diameters of about 2 mm. In some embodiments, the capillary is moved while the first laser marks the capillary.

摘要翻译： 本文公开了蚀刻材料的系统和方法。 所公开的主题的实施例包括用于标记至少一个毛细管的方法，包括用激光蚀刻至少一个毛细管，以及使用第二激光读取所得到的标记的方法。 另外的实施例将第二激光器并入条形码读取器内。 所公开的主题的各种实施例包括外径约2mm的毛细管。 在一些实施例中，当第一激光器标记毛细管时毛细管被移动。

公开(公告)号：US20120132313A1

公开(公告)日：2012-05-31

申请号：US11895557

申请日：2007-08-24

申请人： Anubha Bhatla ,  Alessio Salerno ,  Nabil Simaan ,  Y. Lawrence Yao ,  Gerhard Randers-Pehrson ,  Guy Y. Garty ,  Aparajita Dutta ,  David J. Brenner

发明人： Anubha Bhatla ,  Alessio Salerno ,  Nabil Simaan ,  Y. Lawrence Yao ,  Gerhard Randers-Pehrson ,  Guy Y. Garty ,  Aparajita Dutta ,  David J. Brenner

IPC分类号： B65B3/26

CPC分类号： C40B30/10 ,  C40B60/12

摘要： Systems and methods for cutting materials are disclosed herein In some embodiments, methods of at least partially severing a capillary vessel can include: focusing a laser on a predetermined point on the capillary vessel, said capillary vessel containing a biological sample; and cutting the capillary vessel using a laser at the predetermined point. In some embodiments, the methods further can include capturing an image of the capillary vessel and analyzing the image to determine the predetermined point. In some embodiments, a beam of the laser can be moved using one or more galvanometric mirrors. In some embodiments, the methods further can include cutting a plurality of capillary vessels using the laser. In some embodiments, the methods can include utilizing a plurality of lasers, and/or further can include rotating the capillary vessel while the laser can be cutting the capillary vessel. In some embodiments, cutting the capillary vessel can include cutting only a portion of the capillary vessel.

摘要翻译： 用于切割材料的系统和方法在一些实施例中，至少部分地切断毛细管的方法可以包括：将激光聚焦在毛细血管上的预定点上，所述毛细血管容纳生物样品; 以及在预定点处使用激光切割毛细血管。 在一些实施例中，所述方法还可包括捕获毛细管血管的图像并分析图像以确定预定点。 在一些实施例中，可以使用一个或多个电流计反射镜移动激光束。 在一些实施例中，所述方法还可包括使用所述激光切割多个毛细血管。 在一些实施例中，该方法可以包括利用多个激光器，和/或还可以包括旋转毛细血管，同时激光器可以切割毛细血管。 在一些实施例中，切割毛细血管可包括仅切割毛细血管的一部分。

公开(公告)号：US07787681B2

公开(公告)日：2010-08-31

申请号：US11895485

申请日：2007-08-24

申请人： Jian Zhang ,  Alessio Salerno ,  Nabil Simaan ,  Y. Lawrence Yao ,  Gerhard Randers-Pehrson ,  Guy Garty ,  Aparajita Dutta ,  David J. Brenner

发明人： Jian Zhang ,  Alessio Salerno ,  Nabil Simaan ,  Y. Lawrence Yao ,  Gerhard Randers-Pehrson ,  Guy Garty ,  Aparajita Dutta ,  David J. Brenner

IPC分类号： G06K9/00

CPC分类号： C40B30/10 ,  C40B60/12

摘要： Systems and methods for robotic transport are disclosed herein. In some embodiments, robotic systems for transporting biological samples include: a plurality of capillary vessels, in which each capillary vessel can contain a biological sample from a population; a receptacle that can contain the plurality of capillary vessels; a centrifuge; a first robotic device that can transport the receptacle between an input module and the centrifuge; a second robotic device that can transport the receptacle between the centrifuge and a sample harvest location; a cutting device that can cut each of the plurality of capillary vessels; a multi-well plate having a plurality of wells arranged in an array; and a third robotic device that can transfer at least one portion of each of the plurality of biological samples from each of the plurality of capillary vessels to a corresponding well in the array.

摘要翻译： 本文公开了用于机器人运输的系统和方法。 在一些实施例中，用于输送生物样品的机器人系统包括：多个毛细血管，其中每个毛细血管可以包含来自群体的生物样品; 可容纳多个毛细血管的容器; 离心机 能够在输入模块和离心机之间输送容器的第一机器人装置; 第二机器人装置，其可以在离心机和样品收集位置之间运输容器; 切割装置，其可以切割所述多个毛细血管中的每一个; 具有排列成阵列的多个孔的多孔板; 以及第三机器人装置，其可以将多个生物样品中的每一个的至少一部分从多个毛细血管中的每一个转移到阵列中的对应的孔中。

公开(公告)号：US09662745B2

公开(公告)日：2017-05-30

申请号：US14003319

申请日：2012-03-11

申请人： Y. Lawrence Yao ,  Gen Satoh

发明人： Y. Lawrence Yao ,  Gen Satoh

IPC分类号： B23K26/323 ,  B23K1/19 ,  B23K26/32 ,  B23K1/00 ,  B23K1/005 ,  B23K26/073 ,  B23K26/26 ,  B23K26/0622 ,  B23K101/18 ,  B23K101/32 ,  B23K103/24

CPC分类号： B23K26/323 ,  B23K1/0008 ,  B23K1/0056 ,  B23K1/19 ,  B23K26/0622 ,  B23K26/0732 ,  B23K26/26 ,  B23K2101/185 ,  B23K2101/32 ,  B23K2103/24

摘要： A method, article of manufacture, and system for joining different materials is described. In a principal embodiment, two articles, for example different metals, are placed in proximity to one another with an interfacial area. One article is heated using a laser that is scanned across from a point remote from the interface to a point at or just short of the interface. In embodiments, the interfacial bond region is characterized by a homogenous mixed phase region with very low to substantially no brittle intermetallics.

公开(公告)号：US07958063B2

公开(公告)日：2011-06-07

申请号：US11789571

申请日：2007-04-25

申请人： Xi Long ,  W. Louis Cleveland ,  Y. Lawrence Yao

发明人： Xi Long ,  W. Louis Cleveland ,  Y. Lawrence Yao

IPC分类号： G06N5/00

CPC分类号： G06K9/6269 ,  G06K9/00147 ,  G06K9/6256

摘要： A method of identifying and localizing objects belonging to one of three or more classes, includes deriving vectors, each being mapped to one of the objects, where each of the vectors is an element of an N-dimensional space. The method includes training an ensemble of binary classifiers with a CISS technique, using an ECOC technique. For each object corresponding to a class, the method includes calculating a probability that the associated vector belongs to a particular class, using an ECOC probability estimation technique. In another embodiment, increased detection accuracy is achieved by using images obtained with different contrast methods. A nonlinear dimensional reduction technique, Kernel PCA, was employed to extract features from the multi-contrast composite image. The Kernel PCA preprocessing shows improvements over traditional linear PCA preprocessing possibly due to its ability to capture high-order, nonlinear correlations in the high dimensional image space.

摘要翻译： 一种识别和定位属于三个或更多类中的一个的对象的方法包括导出向量，每个向量被映射到一个对象，其中每个向量是N维空间的元素。 该方法包括使用ECOC技术用CISS技术对二进制分类器进行训练。 对于与类相对应的每个对象，该方法包括使用ECOC概率估计技术来计算关联向量属于特定类的概率。 在另一个实施例中，通过使用用不同对比度方法获得的图像来实现提高的检测精度。 采用非线性尺寸缩小技术Kernel PCA从多重对比度复合图像中提取特征。 内核PCA预处理显示出比传统的线性PCA预处理更好的可能是由于其能够捕获高维非线性相关性的高维图像空间。

公开(公告)号：US07822249B2

公开(公告)日：2010-10-26

申请号：US11895417

申请日：2007-08-24

申请人： Guy Garty ,  David J. Brenner ,  Gerhard Randers-Pehrson ,  Y. Lawrence Yao ,  Nabil Simaan ,  Alessio Salerno ,  Anubha Bhatla ,  Jian Zhang ,  Oleksandra V. Lyulko ,  Aparajita Dutta

发明人： Guy Garty ,  David J. Brenner ,  Gerhard Randers-Pehrson ,  Y. Lawrence Yao ,  Nabil Simaan ,  Alessio Salerno ,  Anubha Bhatla ,  Jian Zhang ,  Oleksandra V. Lyulko ,  Aparajita Dutta

IPC分类号： G06K9/00

CPC分类号： C40B30/10 ,  C40B60/12

摘要： Systems and methods for high-throughput radiation biodosimetry are disclosed herein. In some embodiments, a high-throughput methods of analyzing a population for radiation exposure can include, in various possible sequences: marking a first capillary designed to retain a first sample from the population with a first identifier; transporting a plurality of samples to a biodosimetry system; inputting the samples into the biodosimetry system; centrifuging the plurality of samples including the first sample wherein each sample can be retained in a capillary and the first sample can be retained in the first capillary; transferring the plurality of capillaries including the first capillary from the centrifuge to a cutting device using a robotic device; cutting the first capillary; reading the first identifier; transferring at least one portion of the first sample from the first capillary to a well in an array, wherein the array can include one or more filters in a multi-well plate; correlating the first identifier to a location of the array that includes the at least one portion of the first sample; one or more cycles of biological processing, which can include addition of a reagent and/or incubation of a selected temperature such as, for example, 37° C., 4° C., room temperature, and the like; sealing the array; positioning the array adjacent to an imaging element; focusing the imaging element; capturing an image of the first sample in the array; and analyzing the image to determine whether the first sample indicates a level of radiation exposure exceeding a predetermined threshold.

摘要翻译： 本文公开了用于高通量辐射生物测定法的系统和方法。 在一些实施例中，用于分析用于辐射暴露的群体的高通量方法可以以各种可能的顺序包括：标记设计成用第一标识符从群体保留第一样本的第一毛细管; 将多个样品运送到生物测定系统; 将样品输入生物测定系统; 将包括第一样品的多个样品离心，其中每个样品可以保留在毛细管中，并且第一样品可以保留在第一毛细管中; 使用机器人装置将包括第一毛细管的多个毛细管从离心机转移到切割装置; 切割第一根毛细管; 阅读第一个标识符; 将所述第一样品的至少一部分从所述第一毛细管转移到阵列中的阱，其中所述阵列可以包括多孔板中的一个或多个过滤器; 将所述第一标识符与包括所述第一样本的所述至少一部分的所述阵列的位置相关联; 一个或多个生物处理循环，其可以包括添加试剂和/或选择温度的温育，例如37℃，4℃，室温等; 密封阵列; 将阵列定位成与成像元件相邻; 聚焦成像元件; 捕获阵列中第一个样本的图像; 并分析图像以确定第一样本是否指示超过预定阈值的辐射暴露水平。

公开(公告)号：US20080181473A1

公开(公告)日：2008-07-31

申请号：US11895417

申请日：2007-08-24

申请人： Guy Garty ,  David J. Brenner ,  Gerhard Randers-Pehrson ,  Y. Lawrence Yao ,  Nabil Simaan ,  Alessio Salerno ,  Anubha Bhatla ,  Jian Zhang ,  Oleksandra V. Lyulko ,  Aparajita Dutta

发明人： Guy Garty ,  David J. Brenner ,  Gerhard Randers-Pehrson ,  Y. Lawrence Yao ,  Nabil Simaan ,  Alessio Salerno ,  Anubha Bhatla ,  Jian Zhang ,  Oleksandra V. Lyulko ,  Aparajita Dutta

IPC分类号： G06K9/00

CPC分类号： C40B30/10 ,  C40B60/12

摘要： Systems and methods for high-throughput radiation biodosimetry are disclosed herein. In some embodiments, a high-throughput methods of analyzing a population for radiation exposure can include, in various possible sequences: marking a first capillary designed to retain a first sample from the population with a first identifier; transporting a plurality of samples to a biodosimetry system; inputting the samples into the biodosimetry system; centrifuging the plurality of samples including the first sample wherein each sample can be retained in a capillary and the first sample can be retained in the first capillary; transferring the plurality of capillaries including the first capillary from the centrifuge to a cutting device using a robotic device; cutting the first capillary; reading the first identifier; transferring at least one portion of the first sample from the first capillary to a well in an array, wherein the array can include one or more filters in a multi-well plate; correlating the first identifier to a location of the array that includes the at least one portion of the first sample; one or more cycles of biological processing, which can include addition of a reagent and/or incubation of a selected temperature such as, for example, 37° C., 4° C., room temperature, and the like; sealing the array; positioning the array adjacent to an imaging element; focusing the imaging element; capturing an image of the first sample in the array; and analyzing the image to determine whether the first sample indicates a level of radiation exposure exceeding a predetermined threshold.

摘要翻译： 本文公开了用于高通量辐射生物测定法的系统和方法。 在一些实施例中，用于分析用于辐射暴露的群体的高通量方法可以以各种可能的顺序包括：标记设计成用第一标识符从群体保留第一样本的第一毛细管; 将多个样品运送到生物测定系统; 将样品输入生物测定系统; 将包括第一样品的多个样品离心，其中每个样品可以保留在毛细管中，并且第一样品可以保留在第一毛细管中; 使用机器人装置将包括第一毛细管的多个毛细管从离心机转移到切割装置; 切割第一根毛细管; 阅读第一个标识符; 将所述第一样品的至少一部分从所述第一毛细管转移到阵列中的阱，其中所述阵列可以包括多孔板中的一个或多个过滤器; 将所述第一标识符与包括所述第一样本的所述至少一部分的所述阵列的位置相关联; 一个或多个生物处理循环，其可以包括添加试剂和/或选择温度的温育，例如37℃，4℃，室温等; 密封阵列; 将阵列定位成与成像元件相邻; 聚焦成像元件; 捕获阵列中第一个样本的图像; 并分析图像以确定第一样本是否指示超过预定阈值的辐射暴露水平。

公开(公告)号：US5854751A

公开(公告)日：1998-12-29

申请号：US730330

申请日：1996-10-15

申请人： Paul Di Pietro ,  Y. Lawrence Yao

发明人： Paul Di Pietro ,  Y. Lawrence Yao

IPC分类号： B23K26/03 ,  G06F19/00

CPC分类号： B23K26/38 ,  B23K26/034 ,  B23K26/123

摘要： A system and method for stabilizing the cutting front temperature of a laser beam on a workpiece in order to improve the quality of the laser beam cut. The technique and system models the temperature of a virtual grid of nodes on the workpiece's surface and determines the effect of the laser beam on each node's temperature including the creation of the kerf. An optimization technique is then performed on the results from temperature model which creates a power data profile to indicate the correct level of power which the laser should be operated for every point on the laser cut. The technique and system is especially useful when boundary, turns or holes are required where the cutting conditions change creating excessive heat accumulation.

摘要翻译： 一种用于稳定激光束在工件上的切割前端温度以提高激光束切割质量的系统和方法。 该技术和系统对工件表面上节点的虚拟网格的温度进行建模，并确定激光束对每个节点的温度的影响，包括创建切口。 然后对来自温度模型的结果执行优化技术，该模型产生功率数据轮廓以指示激光器对于激光切割上的每个点应该被操作的正确的功率水平。 当切割条件改变产生过多的热积聚时，需要边界，匝或孔时，该技术和系统特别有用。