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公开(公告)号:US20250122564A1
公开(公告)日:2025-04-17
申请号:US18980743
申请日:2024-12-13
Applicant: 10x Genomics, Inc.
Inventor: Marco Mignardi , Marlon Stoeckius
IPC: C12Q1/6874
Abstract: Provided herein are methods of identifying abundance and location of an RNA in a biological sample using an adaptor sequence and a primer. Also disclosed herein are kits. compositions, and systems that are used to perform the methods.
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公开(公告)号:US20240417783A1
公开(公告)日:2024-12-19
申请号:US18785905
申请日:2024-07-26
Applicant: 10x Genomics, Inc.
Inventor: Jennifer Chew , Marlon Stoeckius
IPC: C12Q1/6837 , C12Q1/48 , G01N1/30
Abstract: Provided herein are methods and kits for binding of an analyte capture sequence to a capture domain of a capture probe.
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公开(公告)号:US20240043925A1
公开(公告)日:2024-02-08
申请号:US18490390
申请日:2023-10-19
Applicant: 10x Genomics, Inc.
Inventor: Zachary Bent , Marlon Stoeckius
IPC: C12Q1/6876
CPC classification number: C12Q1/6876
Abstract: Provided herein are methods of detecting target nucleic acids and uses of the same.
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公开(公告)号:US20230383344A1
公开(公告)日:2023-11-30
申请号:US18448618
申请日:2023-08-11
Applicant: 10x Genomics, Inc.
Inventor: Marlon Stoeckius
IPC: C12Q1/6874 , C12Q1/686 , C12Q1/6876 , C12Q1/48 , C12Q1/6806
CPC classification number: C12Q1/6874 , C12Q1/686 , C12Q1/6876 , C12Q1/485 , C12Q1/6806 , G01N2333/9015 , G01N2333/9126 , G01N2333/922 , C12Y301/26004 , C12Y207/07007 , C12Y605/01001
Abstract: Provided herein are methods of identifying a location of an RNA in a sample that include: (a) contacting the sample with an array comprising capture probes, where a capture probe comprises a capture domain and a spatial barcode; (b) releasing the RNA from the sample; (c) extending a 3′ end of the capture probe using the capture domain-bound RNA as a template; (d) generating nick(s) in the extended capture probe-hybridized RNA and performing random-primed DNA synthesis; (e) performing end repair on the second strand DNA molecule; (f) adding a single adenosine nucleotide to the 3′ end of the extended capture probe; (g) ligating a double-stranded sequencing adaptor to the double-stranded DNA product; and (h) determining all or a part of the sequence of the RNA, and the sequence of the spatial barcode, or complements thereof, and using the determined sequences to identify the location of the RNA in the sample.
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公开(公告)号:US20230183684A1
公开(公告)日:2023-06-15
申请号:US18165721
申请日:2023-02-07
Applicant: 10x Genomics, Inc.
Inventor: Caroline Julie Gallant , Marlon Stoeckius , Katherine Pfeiffer
IPC: C12N15/10 , C12Q1/6837 , C12Q1/6874
CPC classification number: C12N15/1093 , C12Q1/6837 , C12Q1/6874
Abstract: Provided herein are methods, compositions, and kits for removing a portion of a sequence in a member of a nucleic acid library.
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公开(公告)号:US11332790B2
公开(公告)日:2022-05-17
申请号:US17220529
申请日:2021-04-01
Applicant: 10x Genomics, Inc.
Inventor: James Michael Chell , Marlon Stoeckius , Jonathan Alles , Caroline Julie Gallant , Christina Galonska , Felice Alessio Bava , Layla Katiraee
IPC: C12Q1/6876 , C12Q1/6811 , C12Q1/25 , C12Q1/6816 , C12Q1/6841 , C12Q1/6851
Abstract: Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample using RNA-templated ligation.
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公开(公告)号:US20220025447A1
公开(公告)日:2022-01-27
申请号:US17312238
申请日:2019-12-06
Applicant: 10x Genomics, Inc.
Inventor: Augusto Manuel Tentori , Marlon Stoeckius
IPC: C12Q1/6837 , C12Q1/6841
Abstract: The present disclosure relates to materials and methods for generating spatial arrays with gradients and using the generated spatial arrays to identify the location of analytes present in a biological sample.
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公开(公告)号:US20240301488A1
公开(公告)日:2024-09-12
申请号:US18605197
申请日:2024-03-14
Applicant: 10x Genomics, Inc.
Inventor: Marlon Stoeckius
IPC: C12Q1/6874 , C12Q1/48 , C12Q1/6806 , C12Q1/686 , C12Q1/6876
CPC classification number: C12Q1/6874 , C12Q1/485 , C12Q1/6806 , C12Q1/686 , C12Q1/6876 , C12Y207/07007 , C12Y301/26004 , C12Y605/01001 , G01N2333/9015 , G01N2333/9126 , G01N2333/922
Abstract: Provided herein are methods of identifying a location of an RNA in a sample that include: (a) contacting the sample with an array comprising capture probes, where a capture probe comprises a capture domain and a spatial barcode; (b) releasing the RNA from the sample; (c) extending a 3′ end of the capture probe using the capture domain-bound RNA as a template; (d) generating nick(s) in the extended capture probe-hybridized RNA and performing random-primed DNA synthesis; (e) performing end repair on the second strand DNA molecule; (f) adding a single adenosine nucleotide to the 3′ end of the extended capture probe; (g) ligating a double-stranded sequencing adaptor to the double-stranded DNA product; and (h) determining all or a part of the sequence of the RNA, and the sequence of the spatial barcode, or complements thereof, and using the determined sequences to identify the location of the RNA in the sample.
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公开(公告)号:US20240052343A1
公开(公告)日:2024-02-15
申请号:US18492344
申请日:2023-10-23
Applicant: 10x Genomics, Inc.
Inventor: Caroline Julie Gallant , Marlon Stoeckius , Katherine Pfeiffer
IPC: C12N15/10 , C12Q1/6837 , C12Q1/6874
CPC classification number: C12N15/1093 , C12Q1/6837 , C12Q1/6874
Abstract: Provided herein are methods, compositions, and kits for removing a portion of a sequence in a member of a nucleic acid library.
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公开(公告)号:US11840687B2
公开(公告)日:2023-12-12
申请号:US18319889
申请日:2023-05-18
Applicant: 10x Genomics, Inc.
Inventor: Caroline Julie Gallant , Marlon Stoeckius , Katherine Pfeiffer
IPC: C12Q1/6837 , C12Q1/6874 , C12N15/10
CPC classification number: C12N15/1093 , C12Q1/6837 , C12Q1/6874
Abstract: Provided herein are methods, compositions, and kits for removing a portion of a sequence in a member of a nucleic acid library.
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