公开(公告)号：US20140287472A1

公开(公告)日：2014-09-25

申请号：US14301438

申请日：2014-06-11

Applicant: AJINOMOTO CO., INC.

Inventor： Konstantin Vyacheslavovich Rybak ,  Ekaterina Aleksandrovna Slivinskaya ,  Ekaterina Alekseevna Savrasova ,  Valeriy Zavenovich Akhverdian ,  Elena Vitalievna Klyachko ,  Sergei Vladimirovich Mashko ,  Vera Georgievna Doroshenko ,  Larisa Gotlibovna Airikh ,  Tatyana Viktorovna Leonova ,  Mikhail Markovich Gusyatiner ,  Elvira Borisovna Voroshilova ,  Yury Ivanovich Kozlov ,  Yoshihiko Hara ,  Takuji Ueda

IPC: C12P13/04 ,  C12P13/24 ,  C12P13/10 ,  C12P13/08

CPC classification number: C12P13/04 ,  C12P13/08 ,  C12P13/10 ,  C12P13/24

Abstract: There is disclosed a method for producing L-amino acid, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance an activity of D-xylose permease.

Abstract translation: 公开了使用肠杆菌科的细菌生产L-氨基酸的方法，例如L-苏氨酸，L-赖氨酸，L-组氨酸，L-苯丙氨酸，L-精氨酸或L-谷氨酸，其中细菌 已被修饰以增强D-木糖通透酶的活性。

公开(公告)号：US08753849B2

公开(公告)日：2014-06-17

申请号：US13774153

申请日：2013-02-22

Applicant: Ajinomoto Co., Inc.

Inventor： Yury Ivanovich Kozlov ,  Akito Chinen ,  Hiroshi Izui ,  Yoshihiko Hara ,  Hisashi Yasueda ,  Konstantin Vyacheslavovich Rybak ,  Ekaterina Aleksandrovna Slivinskaya ,  Joanna Yosifovna Katashkina

IPC: C12P13/14

CPC classification number: C12P13/24 ,  C12N9/88 ,  C12P7/42 ,  C12P7/44 ,  C12P7/46 ,  C12P7/62 ,  C12P13/06 ,  C12P13/10 ,  C12P13/12 ,  C12P13/14 ,  C12P13/20 ,  C12Y401/02009 ,  C12Y401/02022

Abstract: The present invention provides a bacterium which has an ability to produce a useful metabolite derived from acetyl-coenzyme A, such as L-glutamic acid, L-glutamine, L-proline, L-arginine, L-leucine, L-cysteine, succinate, and polyhydroxybutyrate, wherein said bacterium is modified so that activities of D-xylulose-5-phosphate phosphoketolase and/or fructose-6-phosphate phosphoketolase are enhanced. The present invention also provides a method for producing the useful metabolite using the bacterium.

Abstract translation: 本发明提供一种具有生产乙酰辅酶A的有用代谢物的能力，例如L-谷氨酸，L-谷氨酰胺，L-脯氨酸，L-精氨酸，L-亮氨酸，L-半胱氨酸，琥珀酸酯 和聚羟基丁酸酯，其中所述细菌被修饰，使得D-木酮糖-5-磷酸磷酸酮糖苷酶和/或果糖-6-磷酸磷酸酮醇酶的活性增强。 本发明还提供了使用该细菌生产有用代谢物的方法。

公开(公告)号：US20150203881A1

公开(公告)日：2015-07-23

申请号：US14668080

申请日：2015-03-25

Applicant: AJINOMOTO CO., INC.

Inventor： Leonid Romanovich Ptitsyn ,  Irina Borisovna Altman ,  Veronika Aleksandrovna Kotliarova ,  Olga Nikolaevna Mokhova ,  Tatyana Abramovna Yampolskaya ,  Yury Ivanovich Kozlov ,  Masaru Terashita ,  Yoshihiro Usuda ,  Kazuhiko Matsui

IPC: C12P13/08 ,  C12P13/06

CPC classification number: C12P13/08 ,  C12N9/0006 ,  C12P13/04 ,  C12P13/06 ,  C12P13/10 ,  C12P13/14 ,  C12P13/222 ,  C12P13/227 ,  C12P13/24 ,  C12Y101/01002

Abstract: A method for producing an L-amino acid is described, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine, L-tryptophan, or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance an activity of a wild-type alcohol dehydrogenase encoded by the adhE gene or a mutant alcohol dehydrogenase which is resistant to aerobic inactivation.

Abstract translation: 例如使用肠杆菌科的细菌来描述L-氨基酸的制造方法，例如L-苏氨酸，L-赖氨酸，L-组氨酸，L-苯丙氨酸，L-精氨酸，L-色氨酸或L-谷氨酸 家族，其中细菌已经被修饰以增强由adhE基因编码的野生型醇脱氢酶的活性或对有氧失活有抗性的突变型醇脱氢酶。

公开(公告)号：US08969048B2

公开(公告)日：2015-03-03

申请号：US14268110

申请日：2014-05-02

Applicant: Ajinomoto Co., Inc.

Inventor： Yury Ivanovich Kozlov ,  Akito Chinen ,  Hiroshi Izui ,  Yoshihiko Hara ,  Hisashi Yasueda ,  Konstantin Vyacheslavovich Rybak ,  Ekaterina Aleksandrovna Slivinskaya ,  Joanna Yosifovna Katashkina

IPC: C12P13/24 ,  C12N9/88 ,  C12P7/46 ,  C12P7/62 ,  C12P13/06 ,  C12P13/10 ,  C12P13/14 ,  C12P7/44 ,  C12P13/12 ,  C12P13/20 ,  C12P7/42

CPC classification number: C12P13/24 ,  C12N9/88 ,  C12P7/42 ,  C12P7/44 ,  C12P7/46 ,  C12P7/62 ,  C12P13/06 ,  C12P13/10 ,  C12P13/12 ,  C12P13/14 ,  C12P13/20 ,  C12Y401/02009 ,  C12Y401/02022

Abstract: The present invention provides a bacterium which has an ability to produce a useful metabolite derived from acetyl-coenzyme A, such as L-glutamic acid, L-glutamine, L-proline, L-arginine, L-leucine, L-cysteine, succinate, and polyhydroxybutyrate, wherein said bacterium is modified so that activities of D-xylulose-5-phosphate phosphoketolase and/or fructose-6-phosphate phosphoketolase are enhanced. The present invention also provides a method for producing the useful metabolite using the bacterium.

Abstract translation: 本发明提供一种具有生产乙酰辅酶A的有用代谢物的能力，例如L-谷氨酸，L-谷氨酰胺，L-脯氨酸，L-精氨酸，L-亮氨酸，L-半胱氨酸，琥珀酸酯 和聚羟基丁酸酯，其中所述细菌被修饰，使得D-木酮糖-5-磷酸磷酸酮糖苷酶和/或果糖-6-磷酸磷酸酮醇酶的活性增强。 本发明还提供了使用该细菌生产有用代谢物的方法。

公开(公告)号：US20140242643A1

公开(公告)日：2014-08-28

申请号：US14268110

申请日：2014-05-02

Applicant: AJINOMOTO CO., INC.

Inventor： Yury Ivanovich Kozlov ,  Akito Chinen ,  Hiroshi Izui ,  Yoshihiko Hara ,  Hisashi Yasueda ,  Konstantin Vyacheslavovich Rybak ,  Ekaterina Aleksandrovna Slivinskaya ,  Joanna Yosifovna Katashkina

IPC: C12P13/24 ,  C12P13/12 ,  C12P7/42 ,  C12P13/06 ,  C12P7/46 ,  C12P13/14 ,  C12P13/10

CPC classification number: C12P13/24 ,  C12N9/88 ,  C12P7/42 ,  C12P7/44 ,  C12P7/46 ,  C12P7/62 ,  C12P13/06 ,  C12P13/10 ,  C12P13/12 ,  C12P13/14 ,  C12P13/20 ,  C12Y401/02009 ,  C12Y401/02022

Abstract: The present invention provides a bacterium which has an ability to produce a useful metabolite derived from acetyl-coenzyme A, such as L-glutamic acid, L-glutamine, L-proline, L-arginine, L-leucine, L-cysteine, succinate, and polyhydroxybutyrate, wherein said bacterium is modified so that activities of D-xylulose-5-phosphate phosphoketolase and/or fructose-6-phosphate phosphoketolase are enhanced. The present invention also provides a method for producing the useful metabolite using the bacterium.

Abstract translation: 本发明提供一种具有生产乙酰辅酶A的有用代谢物的能力，例如L-谷氨酸，L-谷氨酰胺，L-脯氨酸，L-精氨酸，L-亮氨酸，L-半胱氨酸，琥珀酸酯 和聚羟基丁酸酯，其中所述细菌被修饰，使得D-木酮糖-5-磷酸磷酸酮糖苷酶和/或果糖-6-磷酸磷酸酮醇酶的活性增强。 本发明还提供了使用该细菌生产有用代谢物的方法。

公开(公告)号：US20130157321A1

公开(公告)日：2013-06-20

申请号：US13774153

申请日：2013-02-22

Applicant: Ajinomoto Co., Inc.

Inventor： Yury Ivanovich Kozlov ,  Akito Chinen ,  Hiroshi Izui ,  Yoshihiko Hara ,  Hisashi Yasueda ,  Konstantin Vyacheslavovich Rybak ,  Ekaterina Aleksandrovna Slivinskaya ,  Joanna Yosifovna Katashkina

IPC: C12P13/24 ,  C12P13/14 ,  C12P7/46 ,  C12P13/06 ,  C12P7/44 ,  C12P13/20 ,  C12P13/12

CPC classification number: C12P13/24 ,  C12N9/88 ,  C12P7/42 ,  C12P7/44 ,  C12P7/46 ,  C12P7/62 ,  C12P13/06 ,  C12P13/10 ,  C12P13/12 ,  C12P13/14 ,  C12P13/20 ,  C12Y401/02009 ,  C12Y401/02022

Abstract: The present invention provides a bacterium which has an ability to produce a useful metabolite derived from acetyl-coenzyme A, such as L-glutamic acid, L-glutamine, L-proline, L-arginine, L-leucine, L-cysteine, succinate, and polyhydroxybutyrate, wherein said bacterium is modified so that activities of D-xylulose-5-phosphate phosphoketolase and/or fructose-6-phosphate phosphoketolase are enhanced. The present invention also provides a method for producing the useful metabolite using the bacterium.

Abstract translation: 本发明提供一种具有生产乙酰辅酶A的有用代谢物的能力，例如L-谷氨酸，L-谷氨酰胺，L-脯氨酸，L-精氨酸，L-亮氨酸，L-半胱氨酸，琥珀酸酯 和聚羟基丁酸酯，其中所述细菌被修饰，使得D-木酮糖-5-磷酸磷酸酮糖苷酶和/或果糖-6-磷酸磷酸酮醇酶的活性增强。 本发明还提供了使用该细菌生产有用代谢物的方法。