-
公开(公告)号：US10752660B2
公开(公告)日：2020-08-25
申请号：US15356915
申请日：2016-11-21
Applicant: AJINOMOTO CO., INC. , SPIBER INC.
Inventor： Kentaro Nakase
IPC: C12N15/09 , C12N15/70 , C12N1/00 , C12P21/02 , C07K14/435
Abstract: A method for producing a fibroin-like protein is described. A fibroin-like protein is produced by culturing Escherichia coli having a gene encoding the fibroin-like protein in a medium, inducing expression of the gene encoding the fibroin-like protein, and collecting the fibroin-like protein, wherein the cell proliferation after inducing the expression is reduced.
-
公开(公告)号：US20170066805A1
公开(公告)日：2017-03-09
申请号：US15356915
申请日：2016-11-21
Applicant: AJINOMOTO CO., INC. , SPIBER INC.
Inventor： Kentaro Nakase
IPC: C07K14/435 , C12P21/02 , C12N15/70
Abstract: A method for producing a fibroin-like protein is described. A fibroin-like protein is produced by culturing Escherichia coli having a gene encoding the fibroin-like protein in a medium, inducing expression of the gene encoding the fibroin-like protein, and collecting the fibroin-like protein, wherein the cell proliferation after inducing the expression is reduced.
Abstract translation: 描述了一种生产丝心蛋白样蛋白的方法。 通过培养在培养基中培养具有编码丝心蛋白样蛋白质的基因的大肠杆菌，诱导编码丝心蛋白样蛋白质的基因的表达，收集丝心蛋白样蛋白质，产生丝心蛋白样蛋白质，诱导后的细胞增殖 表达式减少。
-
公开(公告)号：US20190016763A1
公开(公告)日：2019-01-17
申请号：US16144562
申请日：2018-09-27
Applicant: AJINOMOTO CO., INC.
Inventor： Manabu Kitazawa , Nao Sugimoto , Riho Kodama , Takeshi Nagahiko , Yoshihiro Ito , Kentaro Nakase , Yoshinori Takashima , Ayako Mine , Yoko Kuwabara , Uno Tagami , Masayuki Sugiki
Abstract: A technique for efficiently culturing cells such as stem cells is provided. Cells such as stem cells are cultured using a cell culture vessel coated with a fibroin-like protein into which a cell adhesion sequence containing RGD (Arg-Gly-Asp) is inserted.
-
Patent Agency Ranking
公开(公告)号：US20180265555A1
公开(公告)日：2018-09-20
申请号：US15989400
申请日：2018-05-25
Applicant: AJINOMOTO CO., INC. , SPIBER INC.
Inventor： Kentaro Nakase , Yoshinori Takashima , Takeshi Nagahiko
IPC: C07K14/435 , C12N15/71 , C12P21/02
CPC classification number: C07K14/43518 , C07K2319/02 , C07K2319/034 , C07K2319/035 , C12N15/09 , C12N15/71 , C12P21/02 , D01F4/02
Abstract: A method for producing a fibroin-like protein is provided. A fibroin-like protein is produced by a method of culturing Escherichia coli having a gene encoding the fibroin-like protein in a medium, inducing expression of the gene encoding the fibroin-like protein, and collecting the fibroin-like protein, wherein accumulation of an organic acid at the time of inducing the expression is reduced, and wherein the gene is expressed under control of a tryptophan promoter.
-
公开(公告)号：US11117937B2
公开(公告)日：2021-09-14
申请号：US15989400
申请日：2018-05-25
Applicant: AJINOMOTO CO., INC. , SPIBER INC.
Inventor： Kentaro Nakase , Yoshinori Takashima , Takeshi Nagahiko
IPC: C12N15/09 , C12N15/71 , D01F4/02 , C07K14/435 , C12P21/02
Abstract: A method for producing a fibroin-like protein is provided. A fibroin-like protein is produced by a method of culturing Escherichia coli having a gene encoding the fibroin-like protein in a medium, inducing expression of the gene encoding the fibroin-like protein, and collecting the fibroin-like protein, wherein accumulation of an organic acid at the time of inducing the expression is reduced, and wherein the gene is expressed under control of a tryptophan promoter.
-
公开(公告)号：US20200339640A1
公开(公告)日：2020-10-29
申请号：US16929658
申请日：2020-07-15
Applicant: AJINOMOTO CO., INC. , SPIBER INC.
Inventor： Kentaro Nakase
IPC: C07K14/435 , C12P21/02 , C12N15/70
Abstract: A method for producing a fibroin-like protein is described. A fibroin-like protein is produced by culturing Escherichia coli having a gene encoding the fibroin-like protein in a medium, inducing expression of the gene encoding the fibroin-like protein, and collecting the fibroin-like protein, wherein the cell proliferation after inducing the expression is reduced.
-
公开(公告)号：US09926348B2
公开(公告)日：2018-03-27
申请号：US15356818
申请日：2016-11-21
Applicant: AJINOMOTO CO., INC. , SPIBER INC.
Inventor： Kentaro Nakase
IPC: C12P21/02 , C07K14/435 , C12N15/70 , C12N15/09
CPC classification number: C07K14/43518 , C12N15/09 , C12N15/70 , C12P21/02
Abstract: A method for producing a fibroin-like protein is described. A fibroin-like protein is produced by culturing Escherichia coli having a gene encoding the fibroin-like protein in a medium, inducing expression of the gene encoding the fibroin-like protein, and collecting the fibroin-like protein, wherein the accumulation of an organic acid at the time of inducing the expression is reduced.
-
公开(公告)号：US20170066804A1
公开(公告)日：2017-03-09
申请号：US15356818
申请日：2016-11-21
Applicant: AJINOMOTO CO., INC. , SPIBER INC.
Inventor： Kentaro Nakase
IPC: C07K14/435 , C12N15/70 , C12P21/02
CPC classification number: C07K14/43518 , C12N15/09 , C12N15/70 , C12P21/02
Abstract: A method for producing a fibroin-like protein is described. A fibroin-like protein is produced by culturing Escherichia coli having a gene encoding the fibroin-like protein in a medium, inducing expression of the gene encoding the fibroin-like protein, and collecting the fibroin-like protein, wherein the accumulation of an organic acid at the time of inducing the expression is reduced.
Abstract translation: 描述了一种生产丝心蛋白样蛋白的方法。 通过在培养基中培养具有编码丝心蛋白样蛋白质的基因的大肠杆菌，诱导编码丝心蛋白样蛋白质的基因的表达，收集丝心蛋白样蛋白质来生成丝心蛋白样蛋白质，其中有机物 诱导表达时的酸降低。
-
-
-
-
-
-
-
Search Results
8
records
(took 0.019 seconds)
