公开(公告)号：US11117937B2

公开(公告)日：2021-09-14

申请号：US15989400

申请日：2018-05-25

Applicant: AJINOMOTO CO., INC. ,  SPIBER INC.

Inventor： Kentaro Nakase ,  Yoshinori Takashima ,  Takeshi Nagahiko

IPC: C12N15/09 ,  C12N15/71 ,  D01F4/02 ,  C07K14/435 ,  C12P21/02

Abstract: A method for producing a fibroin-like protein is provided. A fibroin-like protein is produced by a method of culturing Escherichia coli having a gene encoding the fibroin-like protein in a medium, inducing expression of the gene encoding the fibroin-like protein, and collecting the fibroin-like protein, wherein accumulation of an organic acid at the time of inducing the expression is reduced, and wherein the gene is expressed under control of a tryptophan promoter.

公开(公告)号：US20190016763A1

公开(公告)日：2019-01-17

申请号：US16144562

申请日：2018-09-27

Applicant: AJINOMOTO CO., INC.

Inventor： Manabu Kitazawa ,  Nao Sugimoto ,  Riho Kodama ,  Takeshi Nagahiko ,  Yoshihiro Ito ,  Kentaro Nakase ,  Yoshinori Takashima ,  Ayako Mine ,  Yoko Kuwabara ,  Uno Tagami ,  Masayuki Sugiki

IPC: C07K14/435 ,  C12N15/62 ,  C12N5/00 ,  C07K2/00 ,  C12M3/04 ,  C12N5/071 ,  C12N5/074

Abstract: A technique for efficiently culturing cells such as stem cells is provided. Cells such as stem cells are cultured using a cell culture vessel coated with a fibroin-like protein into which a cell adhesion sequence containing RGD (Arg-Gly-Asp) is inserted.

公开(公告)号：US20180265555A1

公开(公告)日：2018-09-20

申请号：US15989400

申请日：2018-05-25

Applicant: AJINOMOTO CO., INC. ,  SPIBER INC.

Inventor： Kentaro Nakase ,  Yoshinori Takashima ,  Takeshi Nagahiko

IPC: C07K14/435 ,  C12N15/71 ,  C12P21/02

CPC classification number: C07K14/43518 ,  C07K2319/02 ,  C07K2319/034 ,  C07K2319/035 ,  C12N15/09 ,  C12N15/71 ,  C12P21/02 ,  D01F4/02

Abstract: A method for producing a fibroin-like protein is provided. A fibroin-like protein is produced by a method of culturing Escherichia coli having a gene encoding the fibroin-like protein in a medium, inducing expression of the gene encoding the fibroin-like protein, and collecting the fibroin-like protein, wherein accumulation of an organic acid at the time of inducing the expression is reduced, and wherein the gene is expressed under control of a tryptophan promoter.