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    DNA encoding hydantoinase, DNA encoding N-carbamyl-L-amino acid hydrolase, recombinant DNA, transformed cell, method of producing protein, and method of producing optically active amino acid
    4.
    发明申请
    DNA encoding hydantoinase, DNA encoding N-carbamyl-L-amino acid hydrolase, recombinant DNA, transformed cell, method of producing protein, and method of producing optically active amino acid 失效
    编码乙内酰脲的DNA，编码N-氨基甲酰基-L-氨基酸水解酶的DNA，重组DNA，转化细胞，产生蛋白质的方法以及制备光学活性氨基酸的方法

    公开(公告)号：US20030109013A1

    公开(公告)日：2003-06-12

    申请号：US10289360

    申请日：2002-11-07

    Applicant: AJINOMOTO CO. INC

    Inventor： Yasuhiro Takenaka ,  Shunichi Suzuki ,  Norimasa Onishi ,  Kenzo Yokozeki

    IPC: C12P013/04 ,  C12N009/78 ,  C07H021/04 ,  C12P021/02 ,  C12N001/21 ,  C12N015/74

    CPC classification number: C12N9/80 ,  C12N9/86 ,  C12P13/04 ,  C12P41/009 ,  C12Y305/01077 ,  C12Y305/02002

    Abstract: The present invention provides a method of producing optically active amino acids from 5-substituted hydantoin by isolating a hydantoinase gene and an N-carbamyl-L-amino acid hydrolase gene involved in an ability to convert 5-substituted hydantoin or N-carbamylamino acid into optically active amino acids from a microorganism of the genus Microbacterium having the above ability and by improving gene amplification and transcriptional and translational activities thereby preparing a recombinant wherein the amount of the desired enzymes produced is increased. The hydantoinase gene is, for example, a DNA encoding for a protein having a hydantoinase activity, which has the nucleotide sequence set forth in SEQ ID NO:1 in the Sequence. The N-carbamyl-L-amino acid hydrolase gene is, for example, a DNA encoding for a protein having an N-carbamyl-L-amino acid hydrolase activity, which has the nucleotide sequence set forth in SEQ ID NO:3 in the Sequence.

    Abstract translation: 本发明提供了一种通过分离涉及将5-取代的乙内酰脲或N-氨基甲酰基氨基酸转化成5-取代的乙内酰脲或N-氨基甲酰基氨基酸的能力的乙内酰脲酶基因和N-氨基甲酰基-L-氨基酸水解酶基因从5-取代的乙内酰脲生产旋光性氨基酸的方法 具有上述能力的微杆菌属的微生物的旋光活性氨基酸，通过改善基因扩增和转录和翻译活性，从而制备重组体，其中产生的所需酶的量增加。 乙内酰脲酶基因是例如编码具有乙内酰脲酶活性的蛋白质的DNA，其具有序列中SEQ ID NO：1所示的核苷酸序列。 N-氨基甲酰基-L-氨基酸水解酶基因是例如编码具有N-氨基甲酰基-L-氨基酸水解酶活性的蛋白质的DNA，其具有SEQ ID NO：3所示的核苷酸序列 序列。

    Method for producing halo-L-tryptophan
    8.
    发明申请
    Method for producing halo-L-tryptophan 失效
    产生卤代-L-色氨酸的方法

    公开(公告)号：US20010029037A1

    公开(公告)日：2001-10-11

    申请号：US09800885

    申请日：2001-03-08

    Applicant: AJINOMOTO CO., INC.

    Inventor： Norimasa Onishi ,  Kenzo Yokozeki

    IPC: C12P013/22

    CPC classification number: C12P13/227 ,  C12N1/20 ,  Y10S435/822 ,  Y10S435/873

    Abstract: A method for producing halo-L-tryptophan from haloindole, comprising culturing a microorganism in a culture medium and then contacting the microorganism with (a) a mixture comprising haloindole, pyruvic acid and ammonia, or (b) a mixture comprising haloindole, a source of pyruvic acid and ammonia, until the halo-L-tryptophan is produced; and recovering the halo-L-tryptophan.

    Abstract translation: 一种从卤代吲哚生产卤代-L-色氨酸的方法，包括在培养基中培养微生物，然后使微生物与（a）包含卤代吲哚，丙酮酸和氨的混合物接触，或（b）包含卤代吲哚，源 的丙酮酸和氨，直到产生卤代-L-色氨酸; 并回收卤代-L-色氨酸。

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