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    ISOLATED PHOSPHOLIPID-PROTEIN PARTICLES
    7.
    发明申请
    ISOLATED PHOSPHOLIPID-PROTEIN PARTICLES 审中-公开
    分离的磷脂 - 蛋白质颗粒

    公开(公告)号：US20090161828A1

    公开(公告)日：2009-06-25

    申请号：US12333191

    申请日：2008-12-11

    申请人： Federico Katzen ,  Julia Fletcher ,  Wieslaw Kudlicki ,  Joseph Beechem ,  Lilin Wang

    发明人： Federico Katzen ,  Julia Fletcher ,  Wieslaw Kudlicki ,  Joseph Beechem ,  Lilin Wang

    IPC分类号： G01N23/207 ,  C07K14/00 ,  C12N9/10 ,  C12N9/48 ,  C12N9/16

    CPC分类号： C12P21/02 ,  C07K1/02

    摘要： Systems and methods are provided for producing a protein of interest that is typically not amenable to expression in soluble form in in vitro expression systems. In some aspects, the invention provides methods of synthesizing proteins using in vitro protein synthesis systems that include a scaffold protein such as apolipoprotein or an amphipathic alpha helix containing (“AAHC”) protein, in which higher yields of soluble protein are produced than in the absence of the scaffold protein. The scaffold proteins may be provided in an in vitro protein synthesis system associated with lipid or not associated with lipid. The scaffold protein may be provided as a protein per se or may be encoded by a nucleic acid template and co-expressed with the protein of interest. The invention also provides compositions and kits for synthesis of proteins in soluble form, in which the compositions and kits include cell extracts for protein expression and isolation.

    摘要翻译： 提供的系统和方法用于产生通常不适于在体外表达系统中以可溶形式表达的目的蛋白质。 在一些方面，本发明提供了使用体外蛋白质合成系统合成蛋白质的方法，所述体外蛋白质合成系统包括诸如载脂蛋白或含有（“AAHC”）蛋白质的两亲性α螺旋的支架蛋白，其中产生比在 缺少支架蛋白。 支架蛋白质可以提供在与脂质相关的体外蛋白质合成系统中或与脂质不相关。 支架蛋白质可以作为蛋白质本身提供，或者可以由核酸模板编码并与感兴趣的蛋白质共表达。 本发明还提供用于合成可溶形式的蛋白质的组合物和试剂盒，其中组合物和试剂盒包括用于蛋白质表达和分离的细胞提取物。

    Micromixer with overlapping-crisscross entrance
    8.
    发明申请
    Micromixer with overlapping-crisscross entrance 审中-公开
    具有重叠十字交叉入口的微混合器

    公开(公告)号：US20050232076A1

    公开(公告)日：2005-10-20

    申请号：US11107775

    申请日：2005-04-18

    申请人： Jing-Tang Yang ,  Lilin Wang ,  Ker-Jer Huang

    发明人： Jing-Tang Yang ,  Lilin Wang ,  Ker-Jer Huang

    IPC分类号： B01F3/00 ,  B01F5/06 ,  B01F13/00 ,  B81B1/00

    CPC分类号： B01F13/0059 ,  B01F5/061 ,  B01F2005/0621 ,  B01F2005/0636

    摘要： The micromixer with overlapping-crisscross entrance incorporated with the grooved microchannel, is used effectively for mixing two or more fluid streams. The X-shape overlapping-crisscross inlet ports wherein two microfluidic channels contact over a small area, allow the fluid streams flow through and create the tumbling inside the micromixer. Then merging with some patterned grooves on the walls also induces swirling motion. As a result, the folding and stretching effects of the flow are augmented to amplify the fluid mixing of two or more streams of the inlet fluids within a relative short distance in the micromixer. All of the flow streams are actuated with either pressure driven by a syringe pump or capillary electrophoresis. The present invention is applicable for micro total analysis systems and drug delivery systems.

    摘要翻译： 具有与沟槽微通道结合的交叠十字交叉入口的微混合器被有效地用于混合两个或更多个流体流。 X形交叠十字交叉入口，其中两个微流体通道在小区域上接触，允许流体流流过并在微型混合器内部产生翻转。 然后与壁上的一些图案化凹槽合并也引起旋转运动。 结果，增加了流动的折叠和拉伸效果，以在微型混合器内相对较短的距离内放大入口流体的两个或更多个流体的流体混合。 所有的流动流由用注射器泵或毛细管电泳驱动的压力致动。 本发明适用于微量总分析系统和药物递送系统。

    ISOLATED PHOSPHOLIPID-PROTEIN PARTICLES
    9.
    发明申请
    ISOLATED PHOSPHOLIPID-PROTEIN PARTICLES 审中-公开
    分离的磷脂 - 蛋白质颗粒

    公开(公告)号：US20100233782A1

    公开(公告)日：2010-09-16

    申请号：US12576880

    申请日：2009-10-09

    申请人： Federico Katzen ,  Julia Fletcher ,  Wieslaw Kudlicki ,  Joseph Beechem ,  Lilin Wang

    发明人： Federico Katzen ,  Julia Fletcher ,  Wieslaw Kudlicki ,  Joseph Beechem ,  Lilin Wang

    IPC分类号： C12N9/10 ,  C07K14/00 ,  C12N9/48 ,  C12N9/16

    CPC分类号： C12P21/02 ,  C07K1/02

    摘要： Systems and methods are provided for producing a protein of interest that is typically not amenable to expression in soluble form in in vitro expression systems. In some aspects, the invention provides methods of synthesizing proteins using in vitro protein synthesis systems that include a scaffold protein such as apolipoprotein or an amphipathic alpha helix containing (“AAHC”) protein, in which higher yields of soluble protein are produced than in the absence of the scaffold protein. The scaffold proteins may be provided in an in vitro protein synthesis system associated with lipid or not associated with lipid. The scaffold protein may be provided as a protein per se or may be encoded by a nucleic acid template and co-expressed with the protein of interest. The invention also provides compositions and kits for synthesis of proteins in soluble form, in which the compositions and kits include cell extracts for protein expression and isolation.

    摘要翻译： 提供的系统和方法用于产生通常不适于在体外表达系统中以可溶形式表达的目的蛋白质。 在一些方面，本发明提供了使用体外蛋白质合成系统合成蛋白质的方法，所述体外蛋白质合成系统包括诸如载脂蛋白或含有（“AAHC”）蛋白质的两亲性α螺旋的支架蛋白，其中产生比在 缺少支架蛋白。 支架蛋白质可以提供在与脂质相关的体外蛋白质合成系统中或与脂质不相关。 支架蛋白质可以作为蛋白质本身提供，或者可以由核酸模板编码并与感兴趣的蛋白质共表达。 本发明还提供用于合成可溶形式的蛋白质的组合物和试剂盒，其中组合物和试剂盒包括用于蛋白质表达和分离的细胞提取物。