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9 条记录 (耗时 0.019 秒)

    Silane mixtures
    1.
    发明授权
    Silane mixtures 有权

    公开(公告)号：US10213762B2

    公开(公告)日：2019-02-26

    申请号：US15417847

    申请日：2017-01-27

    申请人： Affymetrix, Inc.

    发明人： Glenn H. McGall ,  Robert G. Kuimelis

    IPC分类号： B01J20/283 ,  B01J20/285 ,  B01J19/00 ,  B82Y30/00 ,  C08G77/16 ,  C08G77/26 ,  C09D183/06 ,  C09D183/08 ,  C40B50/18

    摘要： Silanation compositions containing a mixture of two or more silanation reagents, where at least one silanation reagent includes a functional group capable of supporting polymer synthesis and at least one silanation reagent includes no functional group capable of supporting polymer synthesis are useful in modulating the active site density and hydrolytic stability of a surface. These compositions are particularly useful in silanating a surface prior to preparation of a polymer array and provide for increased hybridization results.

    METHODS AND COMPOSITIONS FOR AMPLIFICATION OF NUCLEIC ACIDS
    2.
    发明申请
    METHODS AND COMPOSITIONS FOR AMPLIFICATION OF NUCLEIC ACIDS 审中-公开
    核酸放大的方法和组合物

    公开(公告)号：US20150159198A1

    公开(公告)日：2015-06-11

    申请号：US14625322

    申请日：2015-02-18

    申请人： Affymetrix, Inc.

    发明人： Glenn H. McGall ,  Anthony D. Barone ,  Christopher J. Kubu

    IPC分类号： C12Q1/68 ,  C12N9/12 ,  C12N9/96

    CPC分类号： C12Q1/6869 ,  C12N9/1241 ,  C12N9/1252 ,  C12N9/96 ,  C12P19/34 ,  C12Q1/686 ,  C12Q2527/125

    摘要： The present invention provides methods, compositions, and kits for storing and enhancing the activity of polymerases and particularly thermostable polymerases. The methods comprise mixing a thermostable polymerase with at least one zwitterionic or ylide surfactant that has at least one PEO group. In another aspect the polymerase is mixed with a blocker such as PLURONIC® or TETRONIC® or an amine N-oxide derivative thereof. The thermostable polymerase may be reversibly inactivated by treatment with 2-(Methylsulfonyl)ethyl 4-nitrophenyl carbonate. Compositions and kits for performing the process according to the invention are also provided.

    摘要翻译： 本发明提供用于储存和增强聚合酶，特别是耐热聚合酶的活性的方法，组合物和试剂盒。 所述方法包括将热稳定聚合酶与至少一种具有至少一个PEO基团的两性离子或内鎓盐表面活性剂混合。 在另一方面，聚合酶与阻断剂例如PLUONIC或TETRONIC或其N-氧化物衍生物混合。 通过用2-（甲基磺酰基）乙基4-硝基苯基碳酸酯处理可以可逆地灭活热稳定聚合酶。 还提供了用于执行根据本发明的方法的组合物和试剂盒。

    Methods and compositions for amplification of nucleic acids
    3.
    发明授权
    Methods and compositions for amplification of nucleic acids 有权
    用于扩增核酸的方法和组合物

    公开(公告)号：US09085761B1

    公开(公告)日：2015-07-21

    申请号：US13791164

    申请日：2013-03-08

    申请人： Affymetrix, Inc.

    发明人： Glenn H. McGall ,  Anthony D. Barone ,  Christopher J. Kubu

    IPC分类号： C12Q1/00 ,  C12Q1/68 ,  C12P19/34 ,  C12N9/12

    CPC分类号： C12Q1/6869 ,  C12N9/1241 ,  C12N9/1252 ,  C12N9/96 ,  C12P19/34 ,  C12Q1/686 ,  C12Q2527/125

    摘要： The present invention provides methods, compositions, and kits for storing and enhancing the activity of polymerases and particularly thermostable polymerases. The methods comprise mixing a thermostable polymerase with at least one zwitterionic or ylide surfactant that has at least one PEO group. In another aspect the polymerase is mixed with a blocker such as PLURONIC® or TETRONIC® or an amine N-oxide derivative thereof. The thermostable polymerase may be reversibly inactivated by treatment with 2-(Methylsulfonyl)ethyl 4-nitrophenyl carbonate. Compositions and kits for performing the process according to the invention are also provided.

    摘要翻译： 本发明提供用于储存和增强聚合酶，特别是耐热聚合酶的活性的方法，组合物和试剂盒。 所述方法包括将热稳定聚合酶与至少一种具有至少一个PEO基团的两性离子或内鎓盐表面活性剂混合。 在另一方面，聚合酶与阻断剂例如PLUONIC或TETRONIC或其N-氧化物衍生物混合。 通过用2-（甲基磺酰基）乙基4-硝基苯基碳酸酯处理可以可逆地灭活热稳定聚合酶。 还提供了用于执行根据本发明的方法的组合物和试剂盒。

    Methods and Compositions for Amplification of Nucleic Acids
    9.
    发明申请
    Methods and Compositions for Amplification of Nucleic Acids 审中-公开
    核酸扩增的方法和组合物

    公开(公告)号：US20150252351A1

    公开(公告)日：2015-09-10

    申请号：US14717724

    申请日：2015-05-20

    申请人： Affymetrix, Inc.

    发明人： Glenn H. McGall ,  Anthony D. Barone ,  Christopher J. Kubu

    IPC分类号： C12N9/96 ,  C12P19/34 ,  C12N9/12

    CPC分类号： C12Q1/6869 ,  C12N9/1241 ,  C12N9/1252 ,  C12N9/96 ,  C12P19/34 ,  C12Q1/686 ,  C12Q2527/125

    摘要： The present invention provides methods, compositions, and kits for storing and enhancing the activity of polymerases and particularly thermostable polymerases. The methods comprise mixing a thermostable polymerase with at least one zwitterionic or ylide surfactant that has at least one PEO group. In another aspect the polymerase is mixed with a blocker such as PLURONIC® or TETRONIC® or an amine N-oxide derivative thereof. The thermostable polymerase may be reversibly inactivated by treatment with 2-(Methylsulfonyl)ethyl 4-nitrophenyl carbonate. Compositions and kits for performing the process according to the invention are also provided.

    摘要翻译： 本发明提供用于储存和增强聚合酶，特别是耐热聚合酶的活性的方法，组合物和试剂盒。 所述方法包括将热稳定聚合酶与至少一种具有至少一个PEO基团的两性离子或内鎓盐表面活性剂混合。 在另一方面，聚合酶与阻断剂例如PLUONIC或TETRONIC或其N-氧化物衍生物混合。 通过用2-（甲基磺酰基）乙基4-硝基苯基碳酸酯处理可以可逆地灭活热稳定聚合酶。 还提供了用于执行根据本发明的方法的组合物和试剂盒。