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16 条记录 (耗时 0.045 秒)

    Absolute quantitation of protein contents based on exponentially modified protein abundance index by mass spectrometry
    5.
    发明授权
    Absolute quantitation of protein contents based on exponentially modified protein abundance index by mass spectrometry 有权
    基于指数变化的蛋白质丰度指数通过质谱法对蛋白质含量进行绝对定量

    公开(公告)号：US07910372B2

    公开(公告)日：2011-03-22

    申请号：US11658592

    申请日：2005-07-04

    申请人： Yasushi Ishihama

    发明人： Yasushi Ishihama

    IPC分类号： G01N33/00 ,  G01N33/48

    CPC分类号： G01N33/6803 ,  G01N33/6848

    摘要： The present inventor has established protein abundance index (PAI, π) to determine the protein contents in a protein mixture solution using nanoLC-MSMS data. Digested peptides were analyzed by nanoLC-MS/MS and the obtained results were applied to a Mascot protein identification algorism based on tandem mass spectra. For absolute quantitation, PAI was converted to exponentially modified PAI (EMPAI, m π), which is proportional to protein contents in the protein mixture. EMPAI was successfully applied to comprehensive protein expression analysis and performed a comparison study between gene and protein expression in an HCT116 human cancer cells. Accordingly, the present invention provides a method and a computer program for quantifying the protein contents based on the protein abundance index.

    摘要翻译： 本发明人利用nanoLC-MSMS数据建立了蛋白质丰度指数（PAI，pgr），以确定蛋白质混合溶液中的蛋白质含量。 通过纳米LC-MS / MS分析消化肽，将得到的结果应用于基于串联质谱的吉祥物蛋白鉴定算法。 为了绝对定量，PAI被转化为指数变化的PAI（EMPAI，m＆pgr），其与蛋白质混合物中的蛋白质含量成比例。 EMPAI成功应用于综合蛋白表达分析，并在HCT116人类癌细胞中进行基因与蛋白质表达的比较研究。 因此，本发明提供了一种用于基于蛋白质丰度指数量化蛋白质含量的方法和计算机程序。

    Quantitation method using isotope labeled internal standard substance, analysis system for executing the quantitation method, and program for the analysis
    6.
    发明申请
    Quantitation method using isotope labeled internal standard substance, analysis system for executing the quantitation method, and program for the analysis 失效
    使用同位素标记的内标物质的定量方法，用于执行定量方法的分析系统和分析程序

    公开(公告)号：US20070134806A1

    公开(公告)日：2007-06-14

    申请号：US10579780

    申请日：2004-11-22

    申请人： Yoshiya Oda ,  Yasushi Ishihama ,  Tsuyoshi Tabata

    发明人： Yoshiya Oda ,  Yasushi Ishihama ,  Tsuyoshi Tabata

    IPC分类号： G01N24/00

    CPC分类号： G01N33/6848 ,  Y10T436/10 ,  Y10T436/104165 ,  Y10T436/104998 ,  Y10T436/105831 ,  Y10T436/24

    摘要： Problem: An object of the present invention is to quantitate with good accuracy, furthermore, quantitate absolutely, one or a plurality of biological molecules in a sample such as a tissue, a biological fluid, a cell, a cell organ or protein complex. Solution: By adding a metabolically isotope labeled biological molecule as an internal standard substance and measuring with a mass spectrometer, quantitating with good accuracy one or a plurality of target molecules in a sample has become possible. In addition, by performing waveform separation processing during mass analysis, a highly accurate quantitative analysis method of mass analysis is provided.

    摘要翻译： 问题：本发明的目的是准确定量，此外，在样品如组织，生物流体，细胞，细胞器官或蛋白质复合物中绝对地定量分析一种或多种生物分子。 解决方案：通过添加代谢同位素标记的生物分子作为内标物质并用质谱仪进行测量，以高精度定量，样品中的一个或多个目标分子成为可能。 此外，通过在质量分析中进行波形分离处理，提供了高精度的质量分析的定量分析方法。

    Capillary having coated inner wall
    8.
    发明授权
    Capillary having coated inner wall 有权
    毛细管涂有内壁

    公开(公告)号：US06586065B1

    公开(公告)日：2003-07-01

    申请号：US09319043

    申请日：1999-06-01

    申请人： Hiroyuki Katayama ,  Yasushi Ishihama

    发明人： Hiroyuki Katayama ,  Yasushi Ishihama

    IPC分类号： G01N2726

    CPC分类号： G01N27/44704 ,  Y10T428/1321 ,  Y10T428/139 ,  Y10T428/31536

    摘要： It is to provide a capillary having adsorbed a polymer. A capillary having absorbed on an inner wall of the capillary, an ionic polymer having positive electric charge and an ionic polymer having negative electric charge alternately, and a process for producing the same.

    摘要翻译： 本发明提供吸附了聚合物的毛细管。毛细管吸收在毛细管内壁上的毛细管，具有正电荷的离子聚合物和交替负电荷的离子聚合物及其制造方法。

    Photohardenable resin composition providing heat-resistant photohardened product
    9.
    发明授权
    Photohardenable resin composition providing heat-resistant photohardened product 有权
    提供耐热光硬化产品的可光硬化树脂组合物

    公开(公告)号：US06413698B1

    公开(公告)日：2002-07-02

    申请号：US09478462

    申请日：2000-01-06

    申请人： Yorikazu Tamura ,  Tsuneo Hagiwara ,  Makoto Ohtake ,  Minoru Shinoda ,  Toshiharu Suzuki ,  Tatsuhiko Ozaki ,  Yasushi Ishihama ,  Takayuki Nakamura

    发明人： Yorikazu Tamura ,  Tsuneo Hagiwara ,  Makoto Ohtake ,  Minoru Shinoda ,  Toshiharu Suzuki ,  Tatsuhiko Ozaki ,  Yasushi Ishihama ,  Takayuki Nakamura

    IPC分类号： G03F7028

    CPC分类号： G03F7/0037 ,  B33Y70/00 ,  C08G18/675 ,  C08L75/16 ,  G03F7/027

    摘要： A photohardenable resin composition comprising (A) a urethanated acrylic compound represented by formula (I): (A)a—C—(R)4−a wherein R is H or alkyl; a is 3 or 4; and A represents a group of formula (II): wherein D represents —CH2CH2O—, —CH2CH(CH3)O—, —CH2CH2COO— or —(CH2)6COO—; E represents —CH2CH2O—, —CH2CH(CH3)O—, —COCH2CH2O— or —CO(CH2)6O—; G represents a diol or triol residual group; J represents a divalent or trivalent and substituted or unsubstituted hydrocarbon group; L represents —(CH2CH2O)h— (h: integer of 1 to 4), —(CH2CH(CH3)O)j— (j: integer of 1 to 4), or —(CH2CH2O)k—(CH2CH(CH3)O)m— (k, m: integer of 1 to 3 (k+m=2, 3 or 4); b, c, and d are each 0 or 1 (b+c+d=1, 2 or 3); e is 0, 1 or 2; f is 0 or 1 (e+f=1 or 2); and g is 1 or 2, and (B) a radical polymerizable compound at a (A) : (B) ratio of 80:20 to 10:90 by weight.

    摘要翻译： 一种可光硬化树脂组合物，其包含（A）由式（I）表示的氨基甲酸酯化丙烯酸化合物：其中R是H或烷基; a是3或4; 并且A表示式（II）的基团：其中D表示-CH 2 CH 2 O-，-CH 2 CH（CH 3）O - ， - CH 2 CH 2 COO-或 - （CH 2）6 COO-; E表示-CH 2 CH 2 O-，-CH 2 CH（CH 3）O-，-COCH 2 CH 2 O-或-CO（CH 2）6 O-; G表示二醇或三醇残基; J表示二价或三价且取代或未取代的烃基; L表示 - （CH 2 CH 2 O）h - （h：1〜4的整数）， - （CH 2 CH（CH 3）O）j-（j：1〜4的整数）或 - （CH 2 CH 2 O）k-（CH 2 CH O）m-（k，m：1〜3的整数（k + m = 2,3或4）; b，c和d分别为0或1（b + c + d = 1,2或3） ; e为0,1或2; f为0或1（e + f = 1或2）; g为1或2，（B）自由基聚合性化合物的（A）：（B） 80:20至10:90（重量）

    Quantitation Method Using Isotope Labeled Internal Standard Substance, Analysis System for Executing the Quantitation Method, and Program for the Analysis
    10.
    发明申请
    Quantitation Method Using Isotope Labeled Internal Standard Substance, Analysis System for Executing the Quantitation Method, and Program for the Analysis 审中-公开
    使用同位素标记的内标物质的定量方法，执行定量方法的分析系统和分析程序

    公开(公告)号：US20110022326A1

    公开(公告)日：2011-01-27

    申请号：US12787712

    申请日：2010-05-26

    申请人： Yoshiya ODA ,  Yasushi Ishihama ,  Tsuyoshi Tabata

    发明人： Yoshiya ODA ,  Yasushi Ishihama ,  Tsuyoshi Tabata

    IPC分类号： G06F19/00 ,  C12N5/07 ,  C12Q1/02 ,  C07C229/08

    CPC分类号： G01N33/6848 ,  Y10T436/10 ,  Y10T436/104165 ,  Y10T436/104998 ,  Y10T436/105831 ,  Y10T436/24

    摘要： Problem: An object of the present invention is to quantitate with good accuracy, furthermore, quantitate absolutely, one or a plurality of biological molecules in a sample such as a tissue, a biological fluid, a cell, a cell organ or protein complex.Solution: By adding a metabolically isotope labeled biological molecule as an internal standard substance and measuring with a mass spectrometer, quantitating with good accuracy one or a plurality of target molecules in a sample has become possible. In addition, by performing waveform separation processing during mass analysis, a highly accurate quantitative analysis method of mass analysis is provided.

    摘要翻译： 问题：本发明的目的是准确定量，此外，在样品如组织，生物流体，细胞，细胞器官或蛋白质复合物中绝对地定量分析一种或多种生物分子。 解决方案：通过添加代谢同位素标记的生物分子作为内标物质并用质谱仪进行测量，以高精度定量，样品中的一个或多个目标分子成为可能。 此外，通过在质量分析中进行波形分离处理，提供了高精度的质量分析的定量分析方法。