公开(公告)号：US07132289B2

公开(公告)日：2006-11-07

申请号：US10015607

申请日：2001-12-17

申请人： Akio Kobayashi ,  Kiichi Fukui ,  Satoshi Harajima ,  Eiichiro Fukusaki ,  Shinichiro Kajiyama ,  Shinya Okuda ,  Takeshi Shoji

发明人： Akio Kobayashi ,  Kiichi Fukui ,  Satoshi Harajima ,  Eiichiro Fukusaki ,  Shinichiro Kajiyama ,  Shinya Okuda ,  Takeshi Shoji

IPC分类号： C12N15/09 ,  C12N5/10 ,  C12N15/63 ,  C12N15/88 ,  A01H5/00

CPC分类号： C12N15/87 ,  C12M35/02 ,  C12N13/00 ,  C12N15/8206

摘要： A method for introducing a foreign matter into a cell, includes the steps of placing a small particle carrying a foreign matter at a part of a cell surface of a living cell, boring a hole in a cell wall and/or a cell membrane by irradiating and treating said part of the cell surface with a laser beam, and introducing the foreign matter into the living cell.

公开(公告)号：US06596540B2

公开(公告)日：2003-07-22

申请号：US09956166

申请日：2001-09-20

申请人： Kiichi Fukui ,  Akio Kobayashi ,  Satoshi Harashima ,  Eiichiro Fukusaki ,  Takefumi Sone

发明人： Kiichi Fukui ,  Akio Kobayashi ,  Satoshi Harashima ,  Eiichiro Fukusaki ,  Takefumi Sone

IPC分类号： C12N1500

CPC分类号： C12N15/89

摘要： A novel method for introduction of an exogenous substance or a physiologically active compound into cells is provided according to this invention. This method can realize introduction of an exogenous genetic substance or a physiologically active compound of large size with a large amount. Such substance is immobilized to beads of sphere fine particles having a particle size of 0.01 mm to 10 mm, and bio-active beads thus produced are introduced into cells. Bio-active beads comprising calcium alginate are particularly useful for the purpose of the present invention.

摘要翻译： 根据本发明提供了将外源物质或生理活性化合物引入细胞的新方法。 该方法可以实现大量外源遗传物质或生理活性化合物的引入。 将该物质固定在粒径为0.01mm〜10mm的球状微粒子珠上，将生成的生物活性珠子导入到细胞中。 包含藻酸钙的生物活性珠特别适用于本发明的目的。

公开(公告)号：US08629980B2

公开(公告)日：2014-01-14

申请号：US13375388

申请日：2010-06-02

申请人： Yasuyuki Ozeki ,  Kazuyoshi Itoh ,  Fumihiro Dake ,  Kiichi Fukui ,  Shinichiro Kajiyama ,  Yuma Kitagawa ,  Norihiko Nishizawa ,  Kazuhiko Sumimura

发明人： Yasuyuki Ozeki ,  Kazuyoshi Itoh ,  Fumihiro Dake ,  Kiichi Fukui ,  Shinichiro Kajiyama ,  Yuma Kitagawa ,  Norihiko Nishizawa ,  Kazuhiko Sumimura

IPC分类号： G01J3/44

CPC分类号： G01J3/4412 ,  G01N21/65 ,  G02B21/06 ,  G02B21/365

摘要： The invention provides an optical microscope that prevents an increase in the complexity of the light source system and is equipped with optics readily capable of adequate operation even when the modulation frequency is increased in order to reduce the impact of the intensity noise of the laser, etc. This optical microscope 100 irradiates a sample 6 with a first train of optical pulses having a first optical frequency, which is generated by a first light source, and a second train of optical pulses having a second optical frequency, which is temporally synchronized with the first train of optical pulses and is generated by a second light source, and detects light scattered from the sample 6. The repetition frequency of the train of optical pulses generated by the first light source is an integral sub-multiple of the repetition frequency of the train of optical pulses generated by the second light source.

摘要翻译： 本发明提供了一种光学显微镜，其防止光源系统的复杂性的增加，并且配备有即使当增加调制频率以便减少激光器的强度噪声的影响等时容易进行适当操作的光学器件 该光学显微镜100照射具有由第一光源产生的具有第一光学频率的第一列光脉冲的样品6和具有与第二光频同步的第二光学频率的第二光脉冲序列 第一光脉冲串，并由第二光源产生，并且检测从样品6散射的光。由第一光源产生的光脉冲序列的重复频率是该第一光源的重复频率的整数倍 由第二光源产生的光脉冲序列。

公开(公告)号：US20120140217A1

公开(公告)日：2012-06-07

申请号：US13375388

申请日：2010-06-02

申请人： Yasuyuki Ozeki ,  Kazuyoshi Itoh ,  Fumihiro Dake ,  Kiichi Fukui ,  Shinichiro Kajiyama ,  Yuma Kitagawa ,  Norihiko Nishizawa ,  Kazuhiko Sumimura

发明人： Yasuyuki Ozeki ,  Kazuyoshi Itoh ,  Fumihiro Dake ,  Kiichi Fukui ,  Shinichiro Kajiyama ,  Yuma Kitagawa ,  Norihiko Nishizawa ,  Kazuhiko Sumimura

IPC分类号： G01N21/65

CPC分类号： G01J3/4412 ,  G01N21/65 ,  G02B21/06 ,  G02B21/365

摘要： The invention provides an optical microscope that prevents an increase in the complexity of the light source system and is equipped with optics readily capable of adequate operation even when the modulation frequency is increased in order to reduce the impact of the intensity noise of the laser, etc. This optical microscope 100 irradiates a sample 6 with a first train of optical pulses having a first optical frequency, which is generated by a first light source, and a second train of optical pulses having a second optical frequency, which is temporally synchronized with the first train of optical pulses and is generated by a second light source, and detects light scattered from the sample 6. The repetition frequency of the train of optical pulses generated by the first light source is an integral sub-multiple of the repetition frequency of the train of optical pulses generated by the second light source.

摘要翻译： 本发明提供了一种光学显微镜，其防止光源系统的复杂性的增加，并且配备有即使当增加调制频率以便减少激光器的强度噪声的影响等时容易进行适当操作的光学器件 该光学显微镜100照射具有由第一光源产生的具有第一光学频率的第一列光脉冲的样品6和具有与第二光频同步的第二光学频率的第二光脉冲序列 第一光脉冲串，并由第二光源产生，并且检测从样品6散射的光。由第一光源产生的光脉冲序列的重复频率是该第一光源的重复频率的整数倍 由第二光源产生的光脉冲序列。

公开(公告)号：US07342223B2

公开(公告)日：2008-03-11

申请号：US11151466

申请日：2005-06-14

申请人： Kunihiko Ohkubo ,  Kiichi Fukui ,  Kazuyoshi Itoh

发明人： Kunihiko Ohkubo ,  Kiichi Fukui ,  Kazuyoshi Itoh

IPC分类号： B01D59/44

CPC分类号： H01J49/162 ,  H01J49/0463

摘要： The mass spectrometer according to the present invention includes a light source for emitting pulse light including a plurality of wavelengths; an ionizer for ionizing molecules of a sample by irradiating the light from the light source to the sample; and a mass analyzer for separating ions ionized in the ionizer according to their mass to charge ratios. For the light source, one including a plurality of ultrashort pulse laser sources each emitting a wavelength different from others, and one emitting ultrashort pulse light including plural wavelengths ranging from the visible region to the infrared region generated by dispersing an ultrashort pulse light with continuous (white) spectrum can be used. Pulse lights having plural wavelengths ranging from near infrared to the ultraviolet region respectively share the role; i.e., one of them vaporizes the sample without fragmenting it, and another ionizes the vaporized sample with the single-photon process or two-photon (or multi-photon) process. This enables ionization of protein complexes as a whole contained in the sample, and enables mass analyses of them.

摘要翻译： 根据本发明的质谱仪包括用于发射包括多个波长的脉冲光的光源; 电离器，用于通过将来自光源的光照射到样品上来使样品的分子离子化; 以及质量分析器，用于根据电离质量与电离比分离在离子发生器中离子化的离子。 对于光源，包括多个发射不同于其他波长的超短脉冲激光源的光源，以及一个发射包括从可见光区域到红外区域的多个波长的超短脉冲光，通过分散具有连续的超短脉冲光 白色）光谱。 分别具有从近红外到紫外线区域的多个波长的脉冲光共同作用; 即，其中一个使样品蒸发而不使其分裂，另一个使用单光子过程或双光子（或多光子）过程将蒸发的样品电离。 这使得能够将样品中包含的蛋白质复合物电离，并且能够进行质量分析。

公开(公告)号：US20100015638A1

公开(公告)日：2010-01-21

申请号：US11887122

申请日：2006-03-24

申请人： Susumu Uchiyama ,  Kiichi Fukui

发明人： Susumu Uchiyama ,  Kiichi Fukui

IPC分类号： G01N33/53

CPC分类号： C07K16/2887 ,  A61K2039/515 ,  C07K2317/92

摘要： Disclosed are a method for determining the affinity of an antibody capable of binding to a cell membrane surface antigen for the antigen; and a method for assaying or screening an antibody capable of binding to a cell membrane surface antigen by utilizing the determination method. In the determination of the affinity of the antibody for the antigen, floating cells presenting the antigen on the surface of the cell membrane thereof are used and the B/F separation of the cells is made by centrifugation or on a filter through which the cells cannot pass.

摘要翻译： 公开了一种确定能够结合抗原的细胞膜表面抗原的抗体的亲和力的方法; 以及通过利用测定方法测定或筛选能够结合细胞膜表面抗原的抗体的方法。 在测定抗体对抗原的亲和性时，使用在其细胞膜表面上呈递抗原的浮游细胞，并通过离心或细胞不能通过的过滤器进行细胞的B / F分离 通过。

公开(公告)号：US20130109874A1

公开(公告)日：2013-05-02

申请号：US13643396

申请日：2011-10-25

申请人： Kiichi Fukui ,  Joyce Cartagena ,  Naoki Wada ,  Tsutomu Kohinata ,  Yasuhisa Yushio ,  Naoki Ikeguchi ,  Satoshi Tabata

发明人： Kiichi Fukui ,  Joyce Cartagena ,  Naoki Wada ,  Tsutomu Kohinata ,  Yasuhisa Yushio ,  Naoki Ikeguchi ,  Satoshi Tabata

IPC分类号： C12N15/82

CPC分类号： C12N15/8247 ,  C12N9/1241 ,  C12N15/8243 ,  C12N15/8271 ,  C12N15/8273 ,  C12Y207/07003

摘要： A PPAT polypeptide of SEQ ID NO: 1 derived from Jatropha, a PPAT polynucleotide of SEQ ID NO: 2 and so on were found. By transforming Jatropha with these PPAT polynucleotides, it is possible to overexpress the PPAT polypeptide in comparison with a wild type, and biosynthesis of coenzyme A is promoted by these polypeptides, the metabolic function and viability of the transformed Jatropha are enhanced, and for example, stress resistance can be significantly improved.

摘要翻译： 发现衍生自麻疯树的SEQ ID NO：1的PPAT多肽，SEQ ID NO：2的PPAT多核苷酸等。 通过用这些PPAT多核苷酸转化麻疯树，可以与野生型相比过表达PPAT多肽，并且这些多肽促进了辅酶A的生物合成，增强了转化的麻疯树的代谢功能和活力，例如， 耐应力可显着提高。

公开(公告)号：US20110263825A1

公开(公告)日：2011-10-27

申请号：US12674783

申请日：2007-09-06

申请人： Susumu Uchiyama ,  Kiichi Fukui

发明人： Susumu Uchiyama ,  Kiichi Fukui

IPC分类号： C07K16/28 ,  C12P21/08

CPC分类号： C07K16/2887 ,  C07K2317/24 ,  C07K2317/56 ,  C07K2317/73 ,  C07K2317/732 ,  C07K2317/734 ,  C07K2317/92

摘要： It is intended to provide a monoclonal antibody having a growth inhibitory activity against a cell having a human CD20 antigen which is produced by using, as immunogens, a human B cell line expressing the human CD20 antigen and a cell line originating in a non-human animal, which is different from an animal to be immunized and has been transformed with human CD20 DNA, and a monoclonal antibody obtained by chimerization or humanization of the above-described monoclonal antibody. These monoclonal antibodies show biological activities suitable for using as drugs.

摘要翻译： 旨在提供对具有人CD20抗原的细胞具有生长抑制活性的单克隆抗体，其通过使用表达人CD20抗原的人B细胞系和源自非人类的细胞系作为免疫原产生 动物，其与被免疫的动物不同，并且已经用人CD20DNA转化，以及通过上述单克隆抗体的嵌合或人源化获得的单克隆抗体。 这些单克隆抗体显示适合用作药物的生物活性。

公开(公告)号：US20110045009A1

公开(公告)日：2011-02-24

申请号：US11883810

申请日：2006-02-09

申请人： Shinsaku Nakagawa ,  Tadanori Mayumi ,  Kiichi Fukui

发明人： Shinsaku Nakagawa ,  Tadanori Mayumi ,  Kiichi Fukui

IPC分类号： A61K39/00 ,  C07K7/08 ,  A61P37/04

CPC分类号： C40B40/02 ,  A61K39/385 ,  A61K47/62 ,  A61K47/645 ,  A61K47/646 ,  A61K2039/6031 ,  C07K7/06 ,  C07K7/08 ,  C07K14/005 ,  C07K2319/00 ,  C07K2319/735 ,  C12N15/1037 ,  C12N2740/16322

摘要： The number of peptides having an ability to bind to a cell or penetrate into a cell is narrowed down by being selectively enriched from a random peptide library with a diversity of not less than one hundred millions of peptides using a phage surface display technique, and then cytoplasmic transfer is evaluated by using protein synthesis inhibition as an indicator by adding to a cell, a fusion body of the selectively enriched peptide and a protein synthesis inhibitory factor (PSIF) that cannot solely penetrate into the cell.

摘要翻译： 通过使用噬菌体表面显示技术从具有不少于一亿多种肽的多样性的随机多肽文库中选择性富集具有结合细胞或穿透细胞的能力的肽的数量变窄，然后 通过添加细胞，选择性富集的肽的融合体和蛋白质合成抑制因子（PSIF）不能仅仅渗入细胞，通过使用蛋白质合成抑制作为指标来评价细胞质转移。

公开(公告)号：US20090232381A1

公开(公告)日：2009-09-17

申请号：US11721120

申请日：2005-10-24

申请人： Sachihiro Matsunaga ,  Susumu Uchiyama ,  Kiichi Fukui

发明人： Sachihiro Matsunaga ,  Susumu Uchiyama ,  Kiichi Fukui

IPC分类号： G06K9/00

CPC分类号： G01N21/6458 ,  G01N21/6428 ,  G01N2021/6423 ,  G01N2021/6482

摘要： A method for monitoring cells is provided capable of determining the stage in cell cycle of a cell in a rapid and highly reliable manner.A step of obtaining an image that reflects the chromosomal state in the cell, and a step of calculating a parameter that corresponds to the chromosomal state based on the image to determine the stage in the cell cycle on the basis of the calculation result are included.

摘要翻译： 提供了一种用于监测细胞的方法，其能够以快速且高度可靠的方式确定细胞的细胞周期的阶段。 包括获得反映细胞中的染色体状态的图像的步骤，以及基于该图像计算与染色体状态对应的参数的步骤，以基于计算结果确定细胞周期中的阶段。