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    Method for production of antibody directed against cell membrane surface antigen epitope and assaying method
    1.
    发明申请
    Method for production of antibody directed against cell membrane surface antigen epitope and assaying method 审中-公开
    生产针对细胞膜表面抗原表位的抗体的方法和测定方法

    公开(公告)号：US20100015638A1

    公开(公告)日：2010-01-21

    申请号：US11887122

    申请日：2006-03-24

    申请人： Susumu Uchiyama ,  Kiichi Fukui

    发明人： Susumu Uchiyama ,  Kiichi Fukui

    IPC分类号： G01N33/53

    CPC分类号： C07K16/2887 ,  A61K2039/515 ,  C07K2317/92

    摘要： Disclosed are a method for determining the affinity of an antibody capable of binding to a cell membrane surface antigen for the antigen; and a method for assaying or screening an antibody capable of binding to a cell membrane surface antigen by utilizing the determination method. In the determination of the affinity of the antibody for the antigen, floating cells presenting the antigen on the surface of the cell membrane thereof are used and the B/F separation of the cells is made by centrifugation or on a filter through which the cells cannot pass.

    摘要翻译： 公开了一种确定能够结合抗原的细胞膜表面抗原的抗体的亲和力的方法; 以及通过利用测定方法测定或筛选能够结合细胞膜表面抗原的抗体的方法。 在测定抗体对抗原的亲和性时，使用在其细胞膜表面上呈递抗原的浮游细胞，并通过离心或细胞不能通过的过滤器进行细胞的B / F分离 通过。

    ANTI-CD20 MONOCLONAL ANTIBODIES
    3.
    发明申请
    ANTI-CD20 MONOCLONAL ANTIBODIES 审中-公开
    抗CD20单克隆抗体

    公开(公告)号：US20110263825A1

    公开(公告)日：2011-10-27

    申请号：US12674783

    申请日：2007-09-06

    申请人： Susumu Uchiyama ,  Kiichi Fukui

    发明人： Susumu Uchiyama ,  Kiichi Fukui

    IPC分类号： C07K16/28 ,  C12P21/08

    CPC分类号： C07K16/2887 ,  C07K2317/24 ,  C07K2317/56 ,  C07K2317/73 ,  C07K2317/732 ,  C07K2317/734 ,  C07K2317/92

    摘要： It is intended to provide a monoclonal antibody having a growth inhibitory activity against a cell having a human CD20 antigen which is produced by using, as immunogens, a human B cell line expressing the human CD20 antigen and a cell line originating in a non-human animal, which is different from an animal to be immunized and has been transformed with human CD20 DNA, and a monoclonal antibody obtained by chimerization or humanization of the above-described monoclonal antibody. These monoclonal antibodies show biological activities suitable for using as drugs.

    摘要翻译： 旨在提供对具有人CD20抗原的细胞具有生长抑制活性的单克隆抗体，其通过使用表达人CD20抗原的人B细胞系和源自非人类的细胞系作为免疫原产生 动物，其与被免疫的动物不同，并且已经用人CD20DNA转化，以及通过上述单克隆抗体的嵌合或人源化获得的单克隆抗体。 这些单克隆抗体显示适合用作药物的生物活性。

    Method for Monitoring Cells, System for Cell-Based Assay, and Program for Cell-Based Assay
    4.
    发明申请
    Method for Monitoring Cells, System for Cell-Based Assay, and Program for Cell-Based Assay 审中-公开
    监测细胞的方法，基于细胞的测定系统和基于细胞的测定的程序

    公开(公告)号：US20090232381A1

    公开(公告)日：2009-09-17

    申请号：US11721120

    申请日：2005-10-24

    申请人： Sachihiro Matsunaga ,  Susumu Uchiyama ,  Kiichi Fukui

    发明人： Sachihiro Matsunaga ,  Susumu Uchiyama ,  Kiichi Fukui

    IPC分类号： G06K9/00

    CPC分类号： G01N21/6458 ,  G01N21/6428 ,  G01N2021/6423 ,  G01N2021/6482

    摘要： A method for monitoring cells is provided capable of determining the stage in cell cycle of a cell in a rapid and highly reliable manner.A step of obtaining an image that reflects the chromosomal state in the cell, and a step of calculating a parameter that corresponds to the chromosomal state based on the image to determine the stage in the cell cycle on the basis of the calculation result are included.

    摘要翻译： 提供了一种用于监测细胞的方法，其能够以快速且高度可靠的方式确定细胞的细胞周期的阶段。 包括获得反映细胞中的染色体状态的图像的步骤，以及基于该图像计算与染色体状态对应的参数的步骤，以基于计算结果确定细胞周期中的阶段。

    Mass spectrometer for biological samples
    7.
    发明授权
    Mass spectrometer for biological samples 失效
    质谱仪用于生物样品

    公开(公告)号：US07342223B2

    公开(公告)日：2008-03-11

    申请号：US11151466

    申请日：2005-06-14

    申请人： Kunihiko Ohkubo ,  Kiichi Fukui ,  Kazuyoshi Itoh

    发明人： Kunihiko Ohkubo ,  Kiichi Fukui ,  Kazuyoshi Itoh

    IPC分类号： B01D59/44

    CPC分类号： H01J49/162 ,  H01J49/0463

    摘要： The mass spectrometer according to the present invention includes a light source for emitting pulse light including a plurality of wavelengths; an ionizer for ionizing molecules of a sample by irradiating the light from the light source to the sample; and a mass analyzer for separating ions ionized in the ionizer according to their mass to charge ratios. For the light source, one including a plurality of ultrashort pulse laser sources each emitting a wavelength different from others, and one emitting ultrashort pulse light including plural wavelengths ranging from the visible region to the infrared region generated by dispersing an ultrashort pulse light with continuous (white) spectrum can be used. Pulse lights having plural wavelengths ranging from near infrared to the ultraviolet region respectively share the role; i.e., one of them vaporizes the sample without fragmenting it, and another ionizes the vaporized sample with the single-photon process or two-photon (or multi-photon) process. This enables ionization of protein complexes as a whole contained in the sample, and enables mass analyses of them.

    摘要翻译： 根据本发明的质谱仪包括用于发射包括多个波长的脉冲光的光源; 电离器，用于通过将来自光源的光照射到样品上来使样品的分子离子化; 以及质量分析器，用于根据电离质量与电离比分离在离子发生器中离子化的离子。 对于光源，包括多个发射不同于其他波长的超短脉冲激光源的光源，以及一个发射包括从可见光区域到红外区域的多个波长的超短脉冲光，通过分散具有连续的超短脉冲光 白色）光谱。 分别具有从近红外到紫外线区域的多个波长的脉冲光共同作用; 即，其中一个使样品蒸发而不使其分裂，另一个使用单光子过程或双光子（或多光子）过程将蒸发的样品电离。 这使得能够将样品中包含的蛋白质复合物电离，并且能够进行质量分析。