公开(公告)号：US20060094085A1

公开(公告)日：2006-05-04

申请号：US11245473

申请日：2005-10-05

申请人： Alan Berry ,  Richard Burlingame ,  James Millis

发明人： Alan Berry ,  Richard Burlingame ,  James Millis

IPC分类号： C12P19/28 ,  C12N1/20

CPC分类号： C07H5/06 ,  C07H11/04 ,  C12N9/1096 ,  C12N15/70 ,  C12P19/26 ,  C12Y206/01016

摘要： The present invention relates to a method and materials for producing glucosamine by fermentation of a genetically modified microorganism. Included in the present invention are genetically modified microorganisms useful in the present method for producing glucsamine, as well as recombinant nucleic acid molecules and the proteins produces by such recombinant nucleic acid molecules.

公开(公告)号：US20100151555A1

公开(公告)日：2010-06-17

申请号：US12540050

申请日：2009-08-12

申请人： Bryan Julien ,  Richard Burlingame

发明人： Bryan Julien ,  Richard Burlingame

IPC分类号： C12N9/10 ,  C12N15/54 ,  C12N15/81 ,  C12N1/19

CPC分类号： C12N9/1085 ,  C12N15/1079 ,  C12P5/002 ,  C12P5/007

摘要： The present invention is directed to variant squalene synthase enzymes, including Saccharomyces cerevisiae squalene synthase enzymes, and to nucleic acid molecules encoding these variant enzymes. These variant enzymes produce squalene at a lower rate than the wild-type enzyme, allowing more farnesyl pyrophosphate to be utilized for production of isoprenoid compounds, while still producing sufficient squalene to allow the S. cerevisiae cells to grow without the requirement for supplementation by sterols such as ergosterol. These variant enzymes, therefore, are highly suitable for the efficient production of isoprenoids.

摘要翻译： 本发明涉及变体角鲨烯合酶，包括酿酒酵母角鲨烯合酶，以及编码这些变体酶的核酸分子。 这些变体酶以比野生型酶低的速率产生角鲨烯，允许更多的法呢基焦磷酸盐用于生产异戊二烯化合物，同时仍然产生足够的角鲨烯以允许酿酒酵母细胞生长而不需要由甾醇补充 如麦角甾醇。 因此，这些变体酶非常适合有效生产类异戊二烯。

公开(公告)号：US09353385B2

公开(公告)日：2016-05-31

申请号：US13987493

申请日：2013-07-30

申请人： Grace Eunyoung Park ,  Bryan N. Julien ,  Richard Burlingame

发明人： Grace Eunyoung Park ,  Bryan N. Julien ,  Richard Burlingame

IPC分类号： C12P7/02 ,  C12P9/00 ,  C12N9/88 ,  C12P7/18

CPC分类号： C12P7/02 ,  C12N9/88 ,  C12P7/18 ,  C12P9/00 ,  C12Y402/03 ,  Y02P20/52

摘要： Provided are labdenediol diphosphate synthase polypeptides, sclareol synthase polypeptides, nucleic acid molecules encoding the labdenediol diphosphate synthase polypeptides and sclareol synthase polypeptides, and methods of using the labdenediol diphosphate synthase polypeptides, sclareol synthase polypeptides. Also provided are methods for producing labdenediol diphosphate, sclareol and (−)-ambroxide.

摘要翻译： 本发明提供了实验二烯二磷酸合酶多肽，香紫苏醇合成酶多肽，编码实二烯二磷酸合成酶多肽和香紫苏醇合成酶多肽的核酸分子，以及使用实验二烯丙二磷酸合成酶多肽，香紫苏醇合成酶多肽的方法。 还提供了生产二氢二苯二酚，香紫苏和（ - ） - 氨氧化物的方法。

公开(公告)号：US20140242658A1

公开(公告)日：2014-08-28

申请号：US14120176

申请日：2014-05-02

申请人： Bryan Julien ,  Richard Burlingame

发明人： Bryan Julien ,  Richard Burlingame

IPC分类号： C12N9/10 ,  C12N15/10 ,  C12P5/00

CPC分类号： C12N9/1085 ,  C12N15/1079 ,  C12P5/002 ,  C12P5/007

摘要： The present invention is directed to variant squalene synthase enzymes, including Saccharomyces cerevisiae squalene synthase enzymes, and to nucleic acid molecules encoding these variant enzymes. These variant enzymes produce squalene at a lower rate than the wild-type enzyme, allowing more farnesyl pyrophosphate to be utilized for production of isoprenoid compounds, while still producing sufficient squalene to allow the S. cerevisiae cells to grow without the requirement for supplementation by sterols such as ergosterol. These variant enzymes, therefore, are highly suitable for the efficient production of isoprenoids.

摘要翻译： 本发明涉及变体角鲨烯合酶，包括酿酒酵母角鲨烯合酶，以及编码这些变体酶的核酸分子。 这些变体酶以比野生型酶低的速率产生角鲨烯，允许更多的法呢基焦磷酸盐用于生产异戊二烯化合物，同时仍然产生足够的角鲨烯以允许酿酒酵母细胞生长而不需要由甾醇补充 如麦角甾醇。 因此，这些变体酶非常适合有效生产类异戊二烯。

公开(公告)号：US08304212B2

公开(公告)日：2012-11-06

申请号：US11775777

申请日：2007-07-10

申请人： Marco A. Baez-Vasquez ,  Richard Burlingame ,  Jon K. Magnuson ,  Marion Bradford ,  Arkady Panteleimonovich Sinitsyn

发明人： Marco A. Baez-Vasquez ,  Richard Burlingame ,  Jon K. Magnuson ,  Marion Bradford ,  Arkady Panteleimonovich Sinitsyn

IPC分类号： C12P19/00 ,  C12N9/00 ,  C12N9/14 ,  C12N1/00

CPC分类号： C12P19/02 ,  C12N9/2428 ,  C12N9/2445 ,  C12P19/20 ,  C12Y302/01003 ,  C12Y302/01021 ,  C12Y302/01037 ,  Y02E50/16

摘要： Methods to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material are provided, as well as novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass.

摘要翻译： 提供了将木质纤维素生物质转化成可降解木质纤维素材料的酶的方法，以及酶的新型组合，包括提供来自植物生物质的糖的协同释放的酶。

公开(公告)号：US20080076159A1

公开(公告)日：2008-03-27

申请号：US11775777

申请日：2007-07-10

申请人： Marco Baez-Vasquez ,  Richard Burlingame ,  Jon Magnuson ,  Marion Bradford ,  Arkady Sinitsyn

发明人： Marco Baez-Vasquez ,  Richard Burlingame ,  Jon Magnuson ,  Marion Bradford ,  Arkady Sinitsyn

IPC分类号： C12P19/00 ,  C12N1/00 ,  C12N9/14 ,  C12P1/02

CPC分类号： C12P19/02 ,  C12N9/2428 ,  C12N9/2445 ,  C12P19/20 ,  C12Y302/01003 ,  C12Y302/01021 ,  C12Y302/01037 ,  Y02E50/16

摘要： Methods to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material are provided, as well as novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass.

摘要翻译： 提供了将木质纤维素生物质转化成可降解木质纤维素材料的酶的方法，以及酶的新型组合，包括提供来自植物生物质的糖的协同释放的酶。

公开(公告)号：US08753842B2

公开(公告)日：2014-06-17

申请号：US13986436

申请日：2013-05-01

申请人： Bryan Julien ,  Richard Burlingame

发明人： Bryan Julien ,  Richard Burlingame

IPC分类号： C12P21/00 ,  C07H21/04 ,  C12P19/34 ,  C12P5/00

CPC分类号： C12N9/1085 ,  C12N15/1079 ,  C12P5/002 ,  C12P5/007

摘要： The present invention is directed to variant squalene synthase enzymes, including Saccharomyces cerevisiae squalene synthase enzymes, and to nucleic acid molecules encoding these variant enzymes. These variant enzymes produce squalene at a lower rate than the wild-type enzyme, allowing more farnesyl pyrophosphate to be utilized for production of isoprenoid compounds, while still producing sufficient squalene to allow the S. cerevisiae cells to grow without the requirement for supplementation by sterols such as ergosterol. These variant enzymes, therefore, are highly suitable for the efficient production of isoprenoids.

公开(公告)号：US20130236943A1

公开(公告)日：2013-09-12

申请号：US13986436

申请日：2013-05-01

申请人： Bryan Julien ,  Richard Burlingame

发明人： Bryan Julien ,  Richard Burlingame

IPC分类号： C12P5/00

CPC分类号： C12N9/1085 ,  C12N15/1079 ,  C12P5/002 ,  C12P5/007

摘要： The present invention is directed to variant squalene synthase enzymes, including Saccharomyces cerevisiae squalene synthase enzymes, and to nucleic acid molecules encoding these variant enzymes. These variant enzymes produce squalene at a lower rate than the wild-type enzyme, allowing more farnesyl pyrophosphate to be utilized for production of isoprenoid compounds, while still producing sufficient squalene to allow the S. cerevisiae cells to grow without the requirement for supplementation by sterols such as ergosterol. These variant enzymes, therefore, are highly suitable for the efficient production of isoprenoids.

摘要翻译： 本发明涉及变体角鲨烯合酶，包括酿酒酵母角鲨烯合酶，以及编码这些变体酶的核酸分子。 这些变体酶以比野生型酶低的速率产生角鲨烯，允许更多的法呢基焦磷酸盐用于生产异戊二烯化合物，同时仍然产生足够的角鲨烯以允许酿酒酵母细胞生长而不需要由甾醇补充 如麦角甾醇。 因此，这些变体酶非常适合有效生产类异戊二烯。

公开(公告)号：US20100151519A1

公开(公告)日：2010-06-17

申请号：US12540094

申请日：2009-08-12

申请人： Bryan Julien ,  Richard Burlingame

发明人： Bryan Julien ,  Richard Burlingame

IPC分类号： C12P21/00 ,  C07H21/04 ,  C12P19/34 ,  C12P5/00

CPC分类号： C12N9/1085 ,  C12N15/1079 ,  C12P5/002 ,  C12P5/007

摘要： The present invention is directed to variant squalene synthase enzymes, including Saccharomyces cerevisiae squalene synthase enzymes, and to nucleic acid molecules encoding these variant enzymes. These variant enzymes produce squalene at a lower rate than the wild-type enzyme, allowing more farnesyl pyrophosphate to be utilized for production of isoprenoid compounds, while still producing sufficient squalene to allow the S. cerevisiae cells to grow without the requirement for supplementation by sterols such as ergosterol. These variant enzymes, therefore, are highly suitable for the efficient production of isoprenoids.

摘要翻译： 本发明涉及变体角鲨烯合酶，包括酿酒酵母角鲨烯合酶，以及编码这些变体酶的核酸分子。 这些变体酶以比野生型酶低的速率产生角鲨烯，允许更多的法呢基焦磷酸盐用于生产异戊二烯化合物，同时仍然产生足够的角鲨烯以允许酿酒酵母细胞生长而不需要由甾醇补充 如麦角甾醇。 因此，这些变体酶非常适合有效生产类异戊二烯。

公开(公告)号：US20070238155A1

公开(公告)日：2007-10-11

申请号：US11487547

申请日：2006-07-13

申请人： Alexander Gusakov ,  Tatyana Salanovich ,  Alexey Antonov ,  Boris Ustinov ,  Oleg Okunev ,  Richard Burlingame ,  Mark Emalfarb ,  Marco Baez ,  Arkady Sinitsyn

发明人： Alexander Gusakov ,  Tatyana Salanovich ,  Alexey Antonov ,  Boris Ustinov ,  Oleg Okunev ,  Richard Burlingame ,  Mark Emalfarb ,  Marco Baez ,  Arkady Sinitsyn

IPC分类号： C12P19/04 ,  A01H5/00 ,  C07H21/00 ,  C07K14/00 ,  C12N1/14 ,  C12P7/24 ,  C12P7/02 ,  C12N9/14 ,  C12P1/00 ,  C12P13/04

CPC分类号： C12N9/2437 ,  C12N9/244 ,  C12N9/2445 ,  C12P19/14 ,  C12Y302/01004 ,  C12Y302/01006 ,  C12Y302/01021 ,  C12Y302/01091 ,  Y02E50/16

摘要： This invention provides novel enzyme compositions using newly identified and isolated C. lucknowense enzymes, including CBH Ib CBH IIb, EG II, EG VI, β-glucosidase, and xylanase II in conjunction with previously identified enzymes CBH Ia, CBH IIa (previously described as Endo 43), and EG V. These enzyme compositions demonstrate an extremely high ability to convert lignocellulosic biomass (e.g., Avicel, cotton, Douglas fir wood pretreated by organosolv) to glucose. CBH Ia and IIb, which both have a cellulose-binding module (CBM) displayed a pronounced synergism with three major endoglucanases (EG II, EG V, EG VI) from the same fungus in hydrolysis of cotton as well as a strong synergy with each other. The enzyme compositions are effective in hydrolysis of the lignocellulosic biomass.

摘要翻译： 本发明提供使用新鉴定和分离的C.运气型酶的新型酶组合物，包括CBH Ib CBH IIb，EG II，EG VI，β-葡糖苷酶和木聚糖酶II结合先前鉴定的酶CBH 1a，CBH IIa（以前描述为 Endo 43）和EG V.这些酶组合物表现出将木质纤维素生物质（例如，通过有机溶剂预处理的Avicel，棉花，Douglas冷杉木材）转化为葡萄糖的极高的能力。 两者具有纤维素结合模块（CBM）的CBH 1a和IIb显示出与来自棉花水解的相同真菌的三种主要内切葡聚糖酶（EG II，EG V，EG VI）的显着协同作用，以及与每种 其他。 酶组合物在木质纤维素生物质的水解中是有效的。