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公开(公告)号:US20130137189A1
公开(公告)日:2013-05-30
申请号:US13745029
申请日:2013-01-18
申请人: Alan J. Polito , Richard M. Thayer , Robert K. DiNello , George H. Sierra , Dennis Nixon , Alan Phillips , Stuart Neubarth
发明人: Alan J. Polito , Richard M. Thayer , Robert K. DiNello , George H. Sierra , Dennis Nixon , Alan Phillips , Stuart Neubarth
IPC分类号: G01N27/22
CPC分类号: G01N27/227 , B01L3/5023 , G01N33/4875 , G01N33/54386 , G01N33/54393 , G01N33/558 , Y10S436/806 , Y10S436/807 , Y10T436/114998
摘要: An embodiment of the present invention provides a method for performing a lateral flow assay. The method includes depositing a sample on a test strip at an application region, detecting a first detection signal arising from the test strip in the first detection zone, and generating a baseline for the first measurement zone by interpolating between values of the detection signal outside of the first measurement zone and inside of the first detection zone. The method may include locating a beginning boundary and an ending boundary for the first measurement zone on the test strip. Additional detection zones having measurement zones may also be incorporated with the embodiment.
摘要翻译: 本发明的一个实施方案提供了一种用于进行侧流测定的方法。 该方法包括将样品沉积在应用区域的测试条上,检测在第一检测区域中由测试条产生的第一检测信号,以及通过在第一检测区域之外的检测信号的值之间进行内插来生成第一测量区域的基线 第一测量区域和第一检测区域内部。 该方法可以包括在测试条上定位用于第一测量区域的开始边界和结束边界。 具有测量区域的附加检测区域也可以结合实施例。
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公开(公告)号:US6136610A
公开(公告)日:2000-10-24
申请号:US199255
申请日:1998-11-23
申请人: Alan J. Polito , Richard M. Thayer , Robert K. DiNello , George H. Sierra , Dennis Nixon , Alan Phillips , Stuart Neubarth
发明人: Alan J. Polito , Richard M. Thayer , Robert K. DiNello , George H. Sierra , Dennis Nixon , Alan Phillips , Stuart Neubarth
IPC分类号: G01N33/543 , G01N33/558 , C12Q1/00 , C12N1/02 , C12Q1/70 , G01N33/53 , G01N35/02
CPC分类号: G01N27/227 , G01N33/54386 , G01N33/54393 , G01N33/558 , B01L3/5023 , G01N33/4875 , Y10S436/806 , Y10S436/807 , Y10T436/114998
摘要: An embodiment of the present invention provides a method for performing a lateral flow assay. The method includes depositing a sample on a test strip at an application region, detecting a first detection signal arising from the test strip in the first detection zone, and generating a baseline for the first measurement zone by interpolating between values of the detection signal outside of the first measurement zone and inside of the first detection zone. The method may include locating a beginning boundary and an ending boundary for the first measurement zone on the test strip. Additional detection zones having measurement zones may also be incorporated with the embodiment.
摘要翻译: 本发明的一个实施方案提供了一种用于进行侧流测定的方法。 该方法包括将样品沉积在应用区域的测试条上,检测在第一检测区域中由测试条产生的第一检测信号,以及通过在第一检测区域之外的检测信号的值之间进行内插来生成第一测量区域的基线 第一测量区域和第一检测区域内部。 该方法可以包括在测试条上定位用于第一测量区域的开始边界和结束边界。 具有测量区域的附加检测区域也可以结合实施例。
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公开(公告)号:US08354270B2
公开(公告)日:2013-01-15
申请号:US11868923
申请日:2007-10-08
申请人: Alan J. Polito , Richard M. Thayer , Robert K. Dinello , George H. Sierra , Dennis Nixon , Alan Phillips , Stuart Neubarth
发明人: Alan J. Polito , Richard M. Thayer , Robert K. Dinello , George H. Sierra , Dennis Nixon , Alan Phillips , Stuart Neubarth
CPC分类号: G01N27/227 , B01L3/5023 , G01N33/4875 , G01N33/54386 , G01N33/54393 , G01N33/558 , Y10S436/806 , Y10S436/807 , Y10T436/114998
摘要: An embodiment of the present invention provides a-method for performing a lateral flow assay. The method includes depositing a sample on a test strip at an application region, detecting a first detection signal arising from the test strip in the first detection zone, and generating a baseline for the first measurement zone by interpolating between values of the detection signal outside of the first measurement zone and inside of the first detection zone. The method may include locating a beginning boundary and an ending boundary for the first measurement zone on the test strip. Additional detection zones having measurement zones may also be incorporated with the embodiment.
摘要翻译: 本发明的一个实施方案提供了一种用于进行侧流测定的方法。 该方法包括将样品沉积在应用区域的测试条上,检测在第一检测区域中由测试条产生的第一检测信号,以及通过在第一检测区域之外的检测信号的值之间进行内插来生成第一测量区域的基线 第一测量区域和第一检测区域内部。 该方法可以包括在测试条上定位用于第一测量区域的开始边界和结束边界。 具有测量区域的附加检测区域也可以结合实施例。
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公开(公告)号:US07297529B2
公开(公告)日:2007-11-20
申请号:US10634331
申请日:2003-08-04
申请人: Alan J. Polito , Richard M. Thayer , Robert K. DiNello , George H. Sierra , Dennis Nixon , Alan Phillips , Stuart Neubarth
发明人: Alan J. Polito , Richard M. Thayer , Robert K. DiNello , George H. Sierra , Dennis Nixon , Alan Phillips , Stuart Neubarth
CPC分类号: G01N27/227 , B01L3/5023 , G01N33/4875 , G01N33/54386 , G01N33/54393 , G01N33/558 , Y10S436/806 , Y10S436/807 , Y10T436/114998
摘要: An embodiment of the present invention provides a method for performing a lateral flow assay. The method includes depositing a sample on a test strip at an application region, detecting a first detection signal arising from the test strip in the first detection zone, and generating a baseline for the first measurement zone by interpolating between values of the detection signal outside of the first measurement zone and inside of the first detection zone. The method may include locating a beginning boundary and an ending boundary for the first measurement zone on the test strip. Additional detection zones having measurement zones may also be incorporated with the embodiment.
摘要翻译: 本发明的一个实施方案提供了一种用于进行侧流测定的方法。 该方法包括将样品沉积在应用区域的测试条上,检测由第一检测区域中的测试条产生的第一检测信号,以及通过在第一测量区域之外的检测信号的值之间进行内插来生成第一测量区域的基线 第一测量区域和第一检测区域内部。 该方法可以包括在测试条上定位用于第一测量区域的开始边界和结束边界。 具有测量区域的附加检测区域也可以结合实施例。
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公开(公告)号:US06528323B1
公开(公告)日:2003-03-04
申请号:US09332933
申请日:1999-06-14
IPC分类号: G01N3353
CPC分类号: G01N33/558 , G01N33/54386 , Y10S435/81 , Y10S435/97 , Y10S435/974 , Y10S435/975 , Y10S436/805 , Y10S436/807 , Y10S436/808 , Y10S436/81 , Y10S436/811 , Y10S436/817
摘要: A test strip adapted to receive a sample and detect an analyte therein is provided. The test strip comprises a sample addition zone to which a sample may be added; an absorbent zone proximal to the sample addition zone; one or more test zones distal to the sample addition zone, at least one of the test zones including a first analyte binding agent immobilized therein which is capable of binding to the analyte to be detected; and a terminal sample flow zone distal to the one or more test zones, the absorbent zone being positioned relative to the sample addition zone and having an absorption capacity relative to the other zones of the test strip such that a distal diffusion front of a sample added to the sample addition zone diffuses from the sample addition zone to a distal diffusion point within the terminal sample flow zone and then reverses direction and diffuses proximal relative to the one or more test zones.
摘要翻译: 提供了适于接收样品并检测其中的分析物的测试条。 测试条包括可以加入样品的样品添加区域; 靠近样品添加区的吸收区; 至少一个测试区域包括固定在其中的能够结合待检测分析物的第一分析物结合剂; 以及远离所述一个或多个测试区域的终端样品流动区域,所述吸收区域相对于所述样品添加区域定位,并且相对于所述测试条带的其它区域具有吸收能力,使得样品的远端扩散前沿 到样品添加区域从样品添加区域扩散到终端样品流动区域内的远端扩散点,然后反转方向并相对于一个或多个测试区域扩散近端。
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公开(公告)号:US07229839B2
公开(公告)日:2007-06-12
申请号:US10346683
申请日:2003-01-17
IPC分类号: G01N33/53
CPC分类号: G01N33/558 , G01N33/54386 , Y10S435/81 , Y10S435/97 , Y10S435/974 , Y10S435/975 , Y10S436/805 , Y10S436/807 , Y10S436/808 , Y10S436/81 , Y10S436/811 , Y10S436/817
摘要: A test strip adapted to receive a sample and detect an analyte therein is provided. The test strip comprises a sample addition zone to which a sample may be added; an absorbent zone proximal to the sample addition zone; one or more test zones distal to the sample addition zone, at least one of the test zones including a first analyte binding agent immobilized therein which is capable of binding to the analyte to be detected; and a terminal sample flow zone distal to the one or more test zones, the absorbent zone being positioned relative to the sample addition zone and having an absorption capacity relative to the other zones of the test strip such that a distal diffusion front of a sample added to the sample addition zone diffuses from the sample addition zone to a distal diffusion point within the terminal sample flow zone and then reverses direction and diffuses proximal relative to the one or more test zones.
摘要翻译: 提供了适于接收样品并检测其中的分析物的测试条。 测试条包括可以加入样品的样品添加区域; 靠近样品添加区的吸收区; 至少一个测试区域包括固定在其中的能够结合待检测分析物的第一分析物结合剂; 以及远离所述一个或多个测试区域的终端样品流动区域,所述吸收区域相对于所述样品添加区域定位,并且相对于所述测试条带的其它区域具有吸收能力,使得样品的远端扩散前沿 到样品添加区域从样品添加区域扩散到终端样品流动区域内的远端扩散点,然后反转方向并相对于一个或多个测试区域扩散近端。
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公开(公告)号:US5688688A
公开(公告)日:1997-11-18
申请号:US288336
申请日:1994-08-10
申请人: Paul A. Luciw , Dino Dina , Steven Rosenberg , Barbara S. Chapman , Richard M. Thayer , Nancy L. Haigwood
发明人: Paul A. Luciw , Dino Dina , Steven Rosenberg , Barbara S. Chapman , Richard M. Thayer , Nancy L. Haigwood
IPC分类号: C12N15/09 , A61K31/00 , A61K38/00 , A61K39/21 , A61K39/395 , A61P31/00 , A61P31/12 , A61P31/14 , A61P31/18 , A61P37/00 , A61P37/02 , C07H21/04 , C07K14/005 , C07K14/155 , C07K14/16 , C07K14/195 , C07K16/00 , C07K19/00 , C12N1/19 , C12N1/21 , C12N7/00 , C12N7/02 , C12N9/02 , C12N15/00 , C12N15/48 , C12N15/49 , C12N15/81 , C12N15/85 , C12P21/02 , C12Q1/68 , C12Q1/70 , C12R1/19 , C12R1/865 , G01N33/53 , G01N33/569 , G01N33/577 , C12N15/67 , C12N15/11
CPC分类号: C07K14/005 , C12N15/81 , C12N15/85 , C12N9/0089 , C12Q1/703 , A61K38/00 , C07K2319/00 , C07K2319/40 , C07K2319/735 , C07K2319/75 , C12N2740/16122 , C12N2740/16222 , C12N2800/108 , C12N2830/00 , C12N2830/002 , C12N2830/005 , C12N2830/15 , C12N2830/36 , C12N2830/55 , C12N2830/702 , C12N2830/704 , Y10S435/81 , Y10S435/82 , Y10S435/974 , Y10S435/975 , Y10S530/826
摘要: Polynucleotide sequences are provided for the diagnosis of the presence of retroviral infection in a human host associated with lymphadenopathy syndrome and/or acquired immune deficiency syndrome, for expression of polypeptides and use of the polypeptides to prepare antibodies, where both the polypeptides and antibodies may be employed as diagnostic reagents or in therapy, e.g., vaccines and passive immunization. The sequences provide detection of the viral infectious agents associated with the indicated syndromes and can be used for expression of antigenic polypeptides.
摘要翻译: 提供多核苷酸序列用于诊断与淋巴结病综合征和/或获得性免疫缺陷综合征相关的人宿主中的逆转录病毒感染的存在,用于表达多肽和使用多肽来制备抗体,其中多肽和抗体可以是 用作诊断试剂或用于治疗,例如疫苗和被动免疫。 这些序列提供与所指示的综合征相关的病毒感染因子的检测,并可用于表达抗原多肽。
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