公开(公告)号：US06379917B1

公开(公告)日：2002-04-30

申请号：US09627642

申请日：2000-07-28

Applicant: Ilya Okun ,  Alex Okun

Inventor： Ilya Okun ,  Alex Okun

IPC: C12Q102

CPC classification number: G01N33/502 ,  G01N33/5008 ,  G01N33/6872 ,  G01N35/00 ,  G01N35/1095 ,  G01N2510/00

Abstract: An apparatus and method for real-time measurement of a cellular response of a test compound or series of test compounds (303) on a flowing suspension of cells (349), in which a homogenous suspension of each member of a series of cell types (349) is combined with a concentration of a test compound (303), directed through a detection zone (355), and a cellular response of the living cells is measured in real time as the cells in the test mixture are flowing through the detection zone (355). The apparatus may be used in automated screening of libraries of compounds, and is capable of real-time variation of concentrations of test and standard compounds and generation of dose/response profiles within a short timespan.

Abstract translation: 一种用于实时测量测试化合物或一系列测试化合物（303）在细胞流动悬浮液（349）上的细胞反应的装置和方法，其中一系列细胞类型的每种成员的均匀悬浮液 349）与测定化合物（303）的浓度结合，通过检测区（355）引导，并且当测试混合物中的细胞流过检测区时，实时测量活细胞的细胞反应 （355）。 该装置可以用于化合物文库的自动筛选，并且能够在短时间内实时测试和标准化合物的浓度变化以及剂量/反应谱的产生。

公开(公告)号：US20070212784A1

公开(公告)日：2007-09-13

申请号：US11683829

申请日：2007-03-08

Applicant: Alex Okun

Inventor： Alex Okun

IPC: G01N35/00

CPC classification number: G01N35/1095 ,  G01N35/1009 ,  G01N35/1065 ,  G01N35/1097 ,  Y10T436/11 ,  Y10T436/117497 ,  Y10T436/118339 ,  Y10T436/119163 ,  Y10T436/2575

Abstract: Disclosed herein is a sample supply device that alternates between the supply of samples from one sample line while cleaning a second sample line and then supplying a second sample from the second sample line while cleaning the first sample line. This is repeated in rapid succession to allow greater speed in analyzing a plurality of samples in a shorter amount of time.

Abstract translation: 本文公开了一种样品供应装置，其在清洁第二样品管线之间从一个样品管线供应的样品之间交替，然后在清洁第一样品管线的同时从第二样品管线提供第二样品。 快速连续重复这一步骤，以便在更短的时间内分析多个样品的速度更快。

公开(公告)号：US06242209B1

公开(公告)日：2001-06-05

申请号：US09568778

申请日：2000-05-10

Applicant: John Ransom ,  Ilya Okun ,  Alex Okun

Inventor： John Ransom ,  Ilya Okun ,  Alex Okun

IPC: C12Q102

CPC classification number: G01N33/6872 ,  G01N15/1404 ,  G01N33/5008 ,  G01N33/502 ,  G01N33/5076 ,  G01N33/5079 ,  G01N33/5302 ,  G01N35/00 ,  G01N2500/00 ,  G01N2510/00 ,  Y10S435/968

Abstract: An apparatus and method for real-time measurement of a cellular response of a test compound or series of test compounds (303) on a flowing suspension of cells (349), in which a homogeneous suspension of each member of a series of cell types (349) is combined with a concentration of a test compound (303), directed through a detection zone (355), and a cellular response of the living cells is measured in real time as the cells in the test mixture are flowing through the detection zone (355). The apparatus may be used in automated screening of libraries of compounds, and is capable of real-time variation of concentrations of test and standard compounds and generation of dose/response profiles within a short time span.

Abstract translation: 一种用于实时测量测试化合物或一系列测试化合物（303）对细胞流动悬浮液（349）的细胞反应的装置和方法，其中一系列细胞类型的每种成员的均匀悬浮液 349）与测定化合物（303）的浓度结合，通过检测区（355）引导，并且当测试混合物中的细胞流过检测区时，实时测量活细胞的细胞反应 （355）。 该装置可以用于化合物文库的自动筛选，并且能够在短时间内实时测试和标准化合物的浓度变化以及剂量/反应谱的产生。

公开(公告)号：US08313948B2

公开(公告)日：2012-11-20

申请号：US12265610

申请日：2008-11-05

Applicant: Alex Okun

Inventor： Alex Okun

IPC: G01N21/05 ,  G01N1/10 ,  G01N35/10

CPC classification number: G01N35/1095 ,  G01N35/1009 ,  G01N35/1065 ,  G01N35/1097 ,  Y10T436/11 ,  Y10T436/117497 ,  Y10T436/118339 ,  Y10T436/119163 ,  Y10T436/2575

Abstract: Disclosed herein is a sample supply device that alternates between the supply of samples from one sample line while cleaning a second sample line and then supplying a second sample from the second sample line while cleaning the first sample line. This is repeated in rapid succession to allow greater speed in analyzing a plurality of samples in a shorter amount of time.

Abstract translation: 本文公开了一种样品供应装置，其在清洁第二样品管线之间从一个样品管线供应的样品之间交替，然后在清洁第一样品管线的同时从第二样品管线提供第二样品。 快速连续重复这一步骤，以便在更短的时间内分析多个样品的速度更快。

公开(公告)号：US07459126B2

公开(公告)日：2008-12-02

申请号：US11683829

申请日：2007-03-08

Applicant: Alex Okun

Inventor： Alex Okun

IPC: G01N21/05 ,  G01N1/10 ,  G01N35/10

CPC classification number: G01N35/1095 ,  G01N35/1009 ,  G01N35/1065 ,  G01N35/1097 ,  Y10T436/11 ,  Y10T436/117497 ,  Y10T436/118339 ,  Y10T436/119163 ,  Y10T436/2575

Abstract: Disclosed herein is a sample supply device that alternates between the supply of samples from one sample line while cleaning a second sample line and then supplying a second sample from the second sample line while cleaning the first sample line. This is repeated in rapid succession to allow greater speed in analyzing a plurality of samples in a shorter amount of time.

Abstract translation: 本文公开了一种样品供应装置，其在清洁第二样品管线之间从一个样品管线供应的样品之间交替，然后在清洁第一样品管线的同时从第二样品管线提供第二样品。 快速连续重复这一步骤，以便在更短的时间内分析多个样品的速度更快。

公开(公告)号：US20050249635A1

公开(公告)日：2005-11-10

申请号：US10841810

申请日：2004-05-07

Applicant: Alex Okun ,  Teresa Bennett ,  Andrew Beemink ,  David Sieg ,  John Ransom

Inventor： Alex Okun ,  Teresa Bennett ,  Andrew Beemink ,  David Sieg ,  John Ransom

IPC: G01N15/14 ,  G01N33/00 ,  G01N35/10

CPC classification number: G01N35/1009 ,  G01N15/1404 ,  G01N2035/1058 ,  Y10T436/114998

Abstract: A system and method of mixing and injecting discrete sample mixtures into a flow cytometer or other sample analysis apparatus may generally comprise a sample injection guide coupling a liquid handling apparatus with a sample analysis apparatus and facilitating injection of discrete sample mixtures into a fluidic system of the apparatus.

Abstract translation: 将离散样品混合物混合和注入到流式细胞仪或其他样品分析装置中的系统和方法通常可以包括将液体处理装置与样品分析装置相结合的样品注入导向件，并且促进将离散样品混合物注入流体系统的流体系统 仪器。

公开(公告)号：US20050021241A1

公开(公告)日：2005-01-27

申请号：US10841974

申请日：2004-05-07

Applicant: Juan Ballesteros ,  Teresa Bennett ,  Alex Okun ,  Andrew Beernink ,  John Ransom

Inventor： Juan Ballesteros ,  Teresa Bennett ,  Alex Okun ,  Andrew Beernink ,  John Ransom

IPC: G01N33/48 ,  G01N33/50 ,  G01N33/537 ,  G01N33/566

CPC classification number: G01N33/5023 ,  G01N33/5008 ,  G01N33/5041 ,  G01N33/537 ,  G01N33/566

Abstract: The present disclosure provides compositions and methods for high throughput Gain of Function (GOF) sorting to discover and develop novel and highly selective candidate drug molecules. High throughput GOF sorting includes: mutating a single residue in a receptor and/or ligand; measuring the affinity and functional activity of the resulting ligand target-ligand interaction; and carrying out multiple rounds of mutation and measurement to determine which residues provide key interaction points underlying the functional activity of a ligand target-ligand interaction. Further, the present disclosure provides methods and compositions for high throughput and high precision GOF sorting, such that large numbers of mutations can be generated and screened rapidly, and GOF compounds can be identified and isolated.

Abstract translation: 本公开提供用于高通量增加功能（GOF）分选以发现和开发新型和高选择性候选药物分子的组合物和方法。 高通量GOF分选包括：突变受体和/或配体中的单个残基; 测量所得配体靶 - 配体相互作用的亲和力和功能活性; 并进行多轮突变和测量以确定哪些残基提供了配体靶 - 配体相互作用的功能活性的关键相互作用点。 此外，本公开提供了用于高通量和高精度GOF分选的方法和组合物，使得可以快速产生和筛选大量突变，并且可以鉴定和分离GOF化合物。

公开(公告)号：US06287758B1

公开(公告)日：2001-09-11

申请号：US09535261

申请日：2000-03-23

Applicant: Ilya Okun ,  Alex Okun ,  Gregory Kaler

Inventor： Ilya Okun ,  Alex Okun ,  Gregory Kaler

IPC: C12Q168

CPC classification number: G01N33/5079 ,  G01N33/5008 ,  G01N33/582 ,  G01N33/6872 ,  Y10T436/25125 ,  Y10T436/25375

Abstract: Sensitive methods for identifying compounds having biological activity comprising combining living cells with two fluorescent membrane permeable ionic dyes having the same charge sign, the first of which has an emission spectrum which overlaps the excitation spectrum of the second fluorescent membrane penetrative dye. The fluorescence is then induced by illuminating the dyes at a wavelength corresponding to the excitation spectrum of the first fluorescent dye and emission is then registered at a wavelength corresponding to the emission spectrum of the second fluorescent dye (FRET). The change in the FRET is indicative of a modulation of cell membrane potential by the biologically active compounds.

Abstract translation: 用于鉴定具有生物活性的化合物的敏感方法包括将活细胞与具有相同电荷符号的两个荧光膜可渗透的离子染料组合，其中第一个具有与第二荧光膜穿透性染料的激发光谱重叠的发射光谱。 然后通过以与第一荧光染料的激发光谱相对应的波长照射染料来诱导荧光，然后以对应于第二荧光染料（FRET）的发射光谱的波长记录发射。 FRET中的变化指示生物活性化合物调节细胞膜电位。

公开(公告)号：US20110065172A1

公开(公告)日：2011-03-17

申请号：US12949493

申请日：2010-11-18

Applicant: Alex Okun ,  Teresa A. Bennett ,  Andrew Beemink ,  David J. Sieg ,  John T. Ransom

Inventor： Alex Okun ,  Teresa A. Bennett ,  Andrew Beemink ,  David J. Sieg ,  John T. Ransom

IPC: C12M1/34

CPC classification number: G01N35/1009 ,  G01N15/1404 ,  G01N2035/1058 ,  Y10T436/114998

Abstract: A system and method of mixing and injecting discrete sample mixtures into a flow cytometer or other sample analysis apparatus may generally comprise a sample injection guide coupling a liquid handling apparatus with a sample analysis apparatus and facilitating injection of discrete sample mixtures into a fluidic system of the apparatus.

Abstract translation: 将离散样品混合物混合和注入到流式细胞仪或其他样品分析装置中的系统和方法通常可以包括将液体处理装置与样品分析装置相结合的样品注入导向件，并且促进将离散样品混合物注入流体系统的流体系统 仪器。

公开(公告)号：US20050249642A1

公开(公告)日：2005-11-10

申请号：US10841239

申请日：2004-05-07

Applicant: Alex Okun ,  John Ransom

Inventor： Alex Okun ,  John Ransom

IPC: B01L99/00 ,  G01N15/14 ,  G01N35/10 ,  B01L11/00

CPC classification number: G01N35/1095 ,  G01N15/1404 ,  G01N2015/1409 ,  G01N2015/149

Abstract: A system and method of mixing and injecting discrete sample mixtures into a flow cytometer or other sample analysis apparatus may generally comprise a sample injection guide coupling a liquid handling apparatus with a sample analysis apparatus and facilitating injection of discrete sample mixtures into a fluidic system of the apparatus.

Abstract translation: 将离散样品混合物混合和注入到流式细胞仪或其他样品分析装置中的系统和方法通常可以包括将液体处理装置与样品分析装置相结合的样品注入导向件，并且促进将离散样品混合物注入流体系统的流体系统 仪器。