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20 条记录 (耗时 0.027 秒)

    Electrophoretic nucleic acid purification method
    5.
    发明授权
    Electrophoretic nucleic acid purification method 失效
    电泳核酸纯化方法

    公开(公告)号：US6146511A

    公开(公告)日：2000-11-14

    申请号：US016531

    申请日：1998-01-30

    申请人： Gary Slater ,  J. William Efcavitch ,  Guy Drouin ,  Pascal Mayer ,  Jean Rousseau ,  Hong Yan Zhou ,  Claudia Chiesa ,  Robert Ruhfel ,  Roger O'Neill

    发明人： Gary Slater ,  J. William Efcavitch ,  Guy Drouin ,  Pascal Mayer ,  Jean Rousseau ,  Hong Yan Zhou ,  Claudia Chiesa ,  Robert Ruhfel ,  Roger O'Neill

    IPC分类号： B01D57/02 ,  B03C5/00 ,  C07H21/02 ,  C07H21/04 ,  C12N15/09 ,  C12N15/10 ,  G01N27/447 ,  G01N27/26

    CPC分类号： C12N15/101 ,  G01N27/447

    摘要： An electrophoretic method for purifying a nucleic acid sample is disclosed. The method generally comprises the steps of (1) providing a nucleic acid sample comprising a desired nucleic acid and one or more contaminants, (2) providing an electrophoresis matrix having a loading well and a recovery well formed therein, (3) placing the nucleic acid sample into the loading well, (4) performing a first electrophoresis comprising electrophoresing the nucleic acid sample for a first time effective to transport the desired nucleic acid out of the loading well and into the electrophoresis matrix; and (5) performing a second electrophoresis comprising electrophoresing the nucleic acid sample for a second time effective to transport the desired nucleic acid out of the electrophoresis matrix and into the recovery well. According to the method, the first and second electrophoresis steps are effective to substantially reduce the concentration of contaminants relative to the concentration of desired nucleic acid in the nucleic acid sample, thereby producing a purified nucleic acid. In the method, the loading and recovery wells may be the same or different, and the electric fields may be DC or alternating. Also disclosed is a preparative electrophoresis method employing an alternating electrical field.

    摘要翻译： 公开了一种用于纯化核酸样品的电泳方法。 该方法通常包括以下步骤：（1）提供包含所需核酸和一种或多种污染物的核酸样品，（2）提供具有载体孔的电泳基质和其中形成的回收孔，（3） （4）进行第一次电泳，其中包括使核酸样品首次有效地将所需核酸转运到载体孔中并进入电泳基质中; 包括进行第二次电泳，其包括将核酸样品电泳第二次，有效将所需核酸转运到电泳基质内并进入回收井。 根据该方法，第一和第二电泳步骤对于相对于核酸样品中所需核酸浓度显着降低污染物的浓度是有效的，从而产生纯化的核酸。 在该方法中，加载和回收井可以相同或不同，并且电场可以是直流或交替的。 还公开了采用交流电场的制备电泳方法。

    Microwave electronic tube with an improved collector
    6.
    发明授权
    Microwave electronic tube with an improved collector 失效
    微波电子管具有改进的收集器

    公开(公告)号：US3940655A

    公开(公告)日：1976-02-24

    申请号：US479136

    申请日：1974-06-13

    申请人： Pierre Palluel ,  Jean Hervier ,  Jean Rousseau

    发明人： Pierre Palluel ,  Jean Hervier ,  Jean Rousseau

    IPC分类号： H01J23/027 ,  H01J25/34

    CPC分类号： H01J23/027

    摘要： In order to reduce the drawbacks, encountered in microwave electronic tubes, resulting from the presence of electrons reflected from the collector of these tubes towards the tube input, the invention provides for the collectors of these tubes to take the form of hollow bodies whose at least part of the internal volume exhibits an axis of symmetry not coinciding with the axis of the beam. This arrangement reduces the number of electrons liable to return towards the tube input and produces a spread in their phases. Application is made to klystrons.

    摘要翻译： 为了减少微波电子管中遇到的缺陷，由于从这些管的收集器反射到电子管输入的电子的存在而产生，本发明提供了这些管的收集器以至少占据中空体的形式， 内部体积的一部分表现出与梁的轴线不一致的对称轴。 这种布置减少了易于返回到管输入的电子的数量并且在其相中产生扩展。 应用于速调管。

    Process for automatically determining the configuration of a stereotactic radiosurgery helmet to which can be fitted a plurality of collimators focused on an irradiation isocenter
    8.
    发明授权
    Process for automatically determining the configuration of a stereotactic radiosurgery helmet to which can be fitted a plurality of collimators focused on an irradiation isocenter 失效
    用于自动确定立体定向放射外科头盔的构造的方法，其可以装配聚焦在照射等角点上的多个准直器

    公开(公告)号：US6044126A

    公开(公告)日：2000-03-28

    申请号：US102216

    申请日：1998-06-22

    申请人： Jean Rousseau ,  David Gibon

    发明人： Jean Rousseau ,  David Gibon

    IPC分类号： A61N5/10

    CPC分类号： A61N5/1031 ,  A61N5/1084

    摘要： Process for determining the configuration or configurations [treatment time (TT.sub.i)/diameter (.phi..sub.i,f) of each collimator] of a helmet for stereotactic radiosurgery, to which can be fitted an plurality of collimators focused on an irradiation isocenter, consisting in automatically optimizing, through iterative dose calculation, the dose (D.sub.p) received at predetermined optimization points (M.sub.p), by modifying, in the course of the successive iterations, the treatment time (TT.sub.i) of at least one shot (i) and the diameter (.phi..sub.i,f) of at least one collimator (C.sub.f) of at least one shot (i), and by calculating, at each iteration, an objective function (OF) having as variables the differences between the calculated dose (D.sub.p) and the expected dose (ED.sub.p) for each point of optimization (M.sub.p), iterative calculation of the doses being carried out automatically until the objective function (OF) satisfies a predetermined optimization criterion.

    摘要翻译： 用于确定用于立体定向放射外科手术的头盔的配置或配置[每个准直器的治疗时间（TTi）/直径（phi i，f）]的过程，其可以安装多个准直器，其聚焦在照射等角点上，其自动组合 通过迭代剂量计算优化在预定优化点（Mp）处接收的剂量（Dp），通过在连续迭代过程中修改至少一个镜头（i）的处理时间（TTi）和直径（ 至少一个准直器（Cf）的至少一个（if）的第i个f i，f），并且通过在每次迭代中计算具有作为变量的目标函数（OF），所述目标函数具有计算剂量（Dp）和 每个优化点（Mp）的预期剂量（EDp），自动执行的剂量的迭代计算，直到目标函数（OF）满足预定的优化标准。

    Liner for deflection yoke
    9.
    发明授权
    Liner for deflection yoke 失效
    内部摆动

    公开(公告)号：US5185672A

    公开(公告)日：1993-02-09

    申请号：US580427

    申请日：1990-09-10

    申请人： Jean Rousseau ,  Claude Richard

    发明人： Jean Rousseau ,  Claude Richard

    IPC分类号： H01J29/82

    CPC分类号： H01J29/826

    摘要： A liner for a deflection yoke assembly for a CRT includes four substantially equally spaced inclined semicircular channel-like mounting studs, arranged about the circumference of a band-like ring about the front of the liner. Threaded sleeves are push mounted upon and ultrasonically welded to an associated mounting stud. Hollow alignment bolts are screwed into each threaded sleeve, and adjustably positioned relative to the cone of a CRT for aligning the yoke to its associated CRT.