公开(公告)号：US20040086426A1

公开(公告)日：2004-05-06

申请号：US10603049

申请日：2003-06-24

Applicant: Applera Corporation

Inventor： Charles S. Vann ,  Dennis Lehto

IPC: B01L003/00

CPC classification number: G01N35/1065 ,  B01J19/0046 ,  B01J2219/00283 ,  B01J2219/00308 ,  B01J2219/00315 ,  B01J2219/00317 ,  B01J2219/00369 ,  B01J2219/00468 ,  B01J2219/005 ,  B01J2219/00545 ,  B01J2219/00549 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/0065 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00689 ,  B01J2219/00691 ,  B01J2219/00693 ,  B01J2219/00702 ,  B01J2219/00722 ,  B01L3/021 ,  B01L3/0289 ,  B01L3/563 ,  B01L2200/021 ,  B01L2200/0647 ,  B01L2200/0657 ,  B01L2200/143 ,  B01L2300/0654 ,  B01L2300/0864 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L2400/049 ,  B82Y30/00 ,  C40B40/06 ,  C40B60/14 ,  C40B70/00 ,  G01N35/10 ,  G01N2035/00574 ,  Y10T436/11 ,  Y10T436/255 ,  Y10T436/2575

Abstract: A bead dispensing system is provided for delivering small amounts of substances onto substrates. The system can include, for example, a movable support structure having an array of spaced-apart projections depending from its lower side. An attraction source, such as a vacuum, magnetic, and/or electrostatic force, is operable at each projection end region to attract and retain one bead. The projection array can be aligned with an array of bead-receiving regions of a substrate, e.g., an array of spaced-apart wells of a micro-plate or card. In one embodiment, a plurality of reagent-carrying beads are picked up, retained at respective projection end regions, and moved to a location over a multi-well plate. The beads are then released in a fashion permitting each bead to land in a respective well. The system of the invention is particularly useful for fabricating arrays of reagents.

Abstract translation: 提供了一种用于将少量物质输送到基底上的珠粒分配系统。 该系统可以包括例如具有从其下侧离开的间隔开的突起阵列的可移动支撑结构。 诸如真空，磁性和/或静电力的吸引源可在每个突出端部区域上操作以吸引并保持一个珠。 投影阵列可以与基板的珠子接收区域的阵列对准，例如微板或卡的间隔开的孔阵列。 在一个实施例中，拾取多个试剂载珠，保持在相应的突出端部区域，并且移动到多孔板上方的位置。 然后以允许每个珠落在相应的孔中的方式释放珠。 本发明的系统对于制造试剂阵列特别有用。

公开(公告)号：US20030205200A1

公开(公告)日：2003-11-06

申请号：US10419563

申请日：2003-04-21

Applicant: Applera Corporation

Inventor： Charles S. Vann ,  Zbigniew T. Bryning

IPC: B05C003/00

CPC classification number: B01L3/0244 ,  B01J19/0046 ,  B01J2219/00369 ,  B01J2219/00376 ,  B01J2219/00387 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00689 ,  B01L3/0251 ,  B01L3/0262 ,  B01L2300/0819 ,  B01L2300/0838 ,  B01L2400/028 ,  B01L2400/0406 ,  C40B60/14 ,  G01N35/1016 ,  G01N35/1067 ,  G01N35/1074 ,  G01N2035/00237 ,  G01N2035/1034 ,  G01N2035/1037 ,  G01N2035/1039 ,  G01N2035/1069 ,  Y10T436/25 ,  Y10T436/2575

Abstract: The present invention provides a method and apparatus for dispensing a small volume of a selected liquid, such as a biological sample or reagent, onto a substrate. The device includes a tube adapted to contain the liquid. An elongate fiber is disposed within the tube for axial movement therein between raised and lowered positions. Upon shifting or oscillating the fiber between its raised and lowered positions, a liquid spot can be formed at a selected position on the substrate. The device is readily adaptable for the production of micro-arrays having a great number of individual spots.