公开(公告)号：US20040234966A1

公开(公告)日：2004-11-25

申请号：US10444848

申请日：2003-05-23

Applicant: Applera Corporation

Inventor： Zbigniew T. Bryning ,  Timothy Z. Liu

IPC: C12Q001/68 ,  C12M001/34

CPC classification number: B01L3/502784 ,  B01L3/50273 ,  B01L2200/0605 ,  B01L2200/0673 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0421 ,  G01N27/44704 ,  G01N27/44747

Abstract: Apparatus and method for handling biological samples. Segments of ionic liquid can provide voltage across segments of immiscible liquid to concentrate or separate charged species in the biological samples. Reactants in biological samples can be contacted and reacted in segments of immiscible liquid.

Abstract translation: 用于处理生物样品的装置和方法。 离子液体的分段可以提供跨混合液体的电压，以浓缩或分离生物样品中的带电物质。 生物样品中的反应物可以与不混溶液体的部分接触并反应。

公开(公告)号：US20030205200A1

公开(公告)日：2003-11-06

申请号：US10419563

申请日：2003-04-21

Applicant: Applera Corporation

Inventor： Charles S. Vann ,  Zbigniew T. Bryning

IPC: B05C003/00

CPC classification number: B01L3/0244 ,  B01J19/0046 ,  B01J2219/00369 ,  B01J2219/00376 ,  B01J2219/00387 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00689 ,  B01L3/0251 ,  B01L3/0262 ,  B01L2300/0819 ,  B01L2300/0838 ,  B01L2400/028 ,  B01L2400/0406 ,  C40B60/14 ,  G01N35/1016 ,  G01N35/1067 ,  G01N35/1074 ,  G01N2035/00237 ,  G01N2035/1034 ,  G01N2035/1037 ,  G01N2035/1039 ,  G01N2035/1069 ,  Y10T436/25 ,  Y10T436/2575

Abstract: The present invention provides a method and apparatus for dispensing a small volume of a selected liquid, such as a biological sample or reagent, onto a substrate. The device includes a tube adapted to contain the liquid. An elongate fiber is disposed within the tube for axial movement therein between raised and lowered positions. Upon shifting or oscillating the fiber between its raised and lowered positions, a liquid spot can be formed at a selected position on the substrate. The device is readily adaptable for the production of micro-arrays having a great number of individual spots.

公开(公告)号：US20030057094A1

公开(公告)日：2003-03-27

申请号：US10281770

申请日：2002-10-27

Applicant: Applera Corporation

Inventor： Zbigniew T. Bryning ,  Theresa B. Taylor

IPC: G01N027/26 ,  G01N027/447

CPC classification number: C07K1/26 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2400/0406 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0487 ,  G01N27/44791

Abstract: A method is disclosed for effecting the concentration of a polar analyte in an alternating electric field. In the method, a relative translation of the polar analyte and an alternating electric field along a translation path is effected. A portion of the polar analyte is then trapped and concentrated in a concentration zone formed by the intersection of the translation path and the alternating electric field. Also disclosed are various devices for carrying out the forgoing method.