公开(公告)号：US5871511A

公开(公告)日：1999-02-16

申请号：US929085

申请日：1997-09-15

申请人： Armin Bolz ,  Klaus Bartels ,  Erhard Flach ,  Rainer Hintsche ,  Manfred Paeschke

发明人： Armin Bolz ,  Klaus Bartels ,  Erhard Flach ,  Rainer Hintsche ,  Manfred Paeschke

IPC分类号： A61N1/362 ,  A61N1/365

CPC分类号： A61N1/3622

摘要： An implantable apparatus for the early diagnosis of tachycardia in the heart is provided witha microelectrode array, which is in contact with the cardiac muscle tissue, for detecting stimulus conduction potentials in the cardiac muscle tissue,a measuring device, which is in connection with the microelectrode array, for determining the refractory time of the cardiac muscle cells in the monitored cardiac region,a measuring device, which is in connection with the microelectrode array, for determining the stimulus conduction velocity in the monitored cardiac muscle region,a computing device for determining the product value of the refractory time and the stimulus conduction velocity,a comparator for comparing the product value with a tachycardia threshold, any falling short of the tachycardia threshold signaling a condition of the heart in risk of tachycardia,a stimulation arrangement for generating antitachycardia stimulation upon detection of a condition of risk of tachycardia in particular via the microelectrode array, anda control unit for controlling the measuring and evaluation processes within the apparatus.

摘要翻译： 用于早期诊断心脏中心动过速的可植入装置设置有与心肌组织接触的微电极阵列，用于检测心肌组织中的刺激传导电位，测量装置与 微电极阵列，用于确定监测的心脏区域中的心肌细胞的难治时间，与微电极阵列相关的测量装置，用于确定所监测的心肌区域中的刺激传导速度;计算装置，用于确定 难治时间和刺激传导速度的乘积值，用于将产品值与心动过速阈值进行比较的比较器，任何低于心动过速阈值的下降信号，表示心脏在心动过速风险中的状况，用于产生抗心动过速刺激的刺激装置 在检测到p的心动过速的风险的情况下 通过微电极阵列关联，以及用于控制装置内的测量和评估过程的控制单元。

公开(公告)号：US5670031A

公开(公告)日：1997-09-23

申请号：US553266

申请日：1995-11-22

申请人： Rainer Hintsche ,  Manfred Paeschke ,  Uwe Schnakenberg ,  Ulla Wollenberger

发明人： Rainer Hintsche ,  Manfred Paeschke ,  Uwe Schnakenberg ,  Ulla Wollenberger

IPC分类号： C12Q1/00 ,  G01F1/704 ,  G01N27/403 ,  G01P5/20 ,  G01N27/26

CPC分类号： G01F1/704 ,  C12Q1/001 ,  G01N27/27 ,  G01P5/20

摘要： The invention relates to an electrochemical sensor with interdigital micro-electrodes (1) with structure widths in the sub-.mu.m range. With a twin or multi-pair array of the interdigital micro-electrodes on a substrate (8), the electrochemical detection of molecules with high sensitivity is made possible and further uses for the detection of chemical reaction cycles are made available. The electrodes may be arranged in a micro-channel applied to the substrate having a constant small volume of a few nanoliters. The electrochemical sensor of the invention is suitable for the multiple measurement of the same species of molecule or as a multi-sensitive sensor in chemical analysis and process control in various fields such as biotechnology, environmental protection or health.

摘要翻译： PCT No.PCT / DE94 / 00598 Sec。 371日期1995年11月22日 102（e）1995年11月22日日期PCT 1994年5月21日PCT PCT。 公开号WO94 / 29708 日期：1994年12月22日本发明涉及一种结构宽度在亚微米范围内的叉指式微电极（1）的电化学传感器。 通过在基板（8）上具有叉指式微电极的双对或多对阵列，使得具有高灵敏度的分子的电化学检测成为可能，并且进一步用于检测化学反应循环。 电极可以布置在施加到具有恒定小体积的几纳升的衬底的微通道中。 本发明的电化学传感器适用于在诸如生物技术，环境保护或健康等各个领域的化学分析和过程控制中相同种类的分子或多感测传感器的多重测量。

公开(公告)号：US5830343A

公开(公告)日：1998-11-03

申请号：US613621

申请日：1996-03-11

申请人： Rainer Hintsche ,  Manfred Paeschke ,  Albrecht Uhlig

发明人： Rainer Hintsche ,  Manfred Paeschke ,  Albrecht Uhlig

IPC分类号： G01N27/403 ,  G01N27/42 ,  G01N27/26

CPC分类号： G01N27/42 ,  G01N27/27

摘要： A process is provided for the electrochemical analysis of compounds of solutions, such as can, e.g., be used in chemical analysis or for control measurements in industrial and environmental sectors. Identification or detection of individual substances takes place by the deposition thereof on electrodes and subsequent detection of the deposited substances. In the process, use is made of a plurality of electrodes. The substances to be identified from the solution are so simultaneously deposited on the electrodes that each electrode is coated with a different substance or substance mixture. Then, the deposited substances are simultaneously identified on the individual electrodes. Thus, the measuring time is significantly shortened and the risk of faulty measurements due to interactions between the substances to be identified on the electrodes is significantly reduced.

摘要翻译： 提供了用于溶液化合物的电化学分析的方法，例如可以用于化学分析或用于工业和环境部门的控制测量。 单个物质的识别或检测是通过在电极上沉积并随后检测沉积物质而进行的。 在该过程中，使用多个电极。 从溶液中鉴定的物质同时沉积在电极上，每个电极涂覆有不同的物质或物质混合物。 然后，在各个电极上同时识别沉积的物质。 因此，测量时间显着缩短，并且由于要在电极上识别的物质之间的相互作用而导致的故障测量的风险显着降低。

公开(公告)号：US06682648B1

公开(公告)日：2004-01-27

申请号：US09105538

申请日：1998-06-26

申请人： Robert D. MacPhee ,  Clive R. Taylor ,  Rainer Hintsche ,  Rene Seitz

发明人： Robert D. MacPhee ,  Clive R. Taylor ,  Rainer Hintsche ,  Rene Seitz

IPC分类号： G02N2726

CPC分类号： C12Q1/005 ,  C12Q1/6825 ,  G01N27/3277 ,  G01N33/5438

摘要： An immunochemical and molecular biologic endpoint reporter system in which reaction products, coupled to electrochemically active molecules susceptible to redox recycling or coupled to enzymes capable of proportional generation of said electrochemically active molecules, are detected and/or quantitated using amperiometry in conjunction with a silicon microchip possessing a closely spaced interdigitated array of nobel metal electrodes.

摘要翻译： 一种免疫化学和分子生物学终点报告系统，其中使用与硅微芯片结合的Amperionometry来检测和/或定量反应产物，其耦合到对氧化还原循环敏感的电化学活性分子或与能够成比例地产生所述电化学活性分子的酶偶联 具有紧密间隔的诺贝尔金属电极的交错排列。

公开(公告)号：US07208077B1

公开(公告)日：2007-04-24

申请号：US09958633

申请日：2000-04-14

申请人： Joerg Albers ,  Helmut Bernt ,  Reinhard Bredehorst ,  Rainer Hintsche ,  René Seitz

发明人： Joerg Albers ,  Helmut Bernt ,  Reinhard Bredehorst ,  Rainer Hintsche ,  René Seitz

IPC分类号： G01N27/403 ,  G01N27/327

CPC分类号： G01N27/3277

摘要： An electric sensor array which is provided with several sensor positions that each have at least two microelectrodes. Molecular substances can be detected electrochemically and charged molecules can be transported or handled using the array. Measuring procedures can be effected, especially using two addressing procedures, in which sensor positions can be individually addressed and electrochemically or electrically controlled by pairing or in groups for voltage or impedance measurements. For biomolecular assays, affinity-binding molecules can be immobilized at the sensor positions, between the microelectrodes, or on auxiliary surfaces.

摘要翻译： 电传感器阵列具有多个传感器位置，每个传感器位置具有至少两个微电极。 分子物质可以电化学检测，带电分子可以使用阵列进行运输或处理。 可以实现测量程序，特别是使用两个寻址过程，其中传感器位置可以单独寻址，并通过配对或电压或电压控制电压或阻抗测量。 对于生物分子测定，亲和力结合分子可以固定在传感器位置，微电极之间或辅助表面上。

公开(公告)号：US06881379B1

公开(公告)日：2005-04-19

申请号：US09958807

申请日：2000-04-14

申请人： Reinhard Bredehorst ,  Rainer Hintsche ,  Rene Seitz ,  Walter Gumbrecht

发明人： Reinhard Bredehorst ,  Rainer Hintsche ,  Rene Seitz ,  Walter Gumbrecht

IPC分类号： B01J19/00 ,  G01N33/50

CPC分类号： B82Y30/00 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00378 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00704 ,  G01N27/27 ,  G01N27/3276

摘要： The invention relates to a method for producing a detection system for detecting different analytes in a sample, characterised by the following steps: providing a planar or essentially planar substrate which has sensors for chemically, optically or electrically detecting the analytes; applying an already microstructured layer to the substrate or applying a continuous layer to the substrate and microstructuring said layer, the layer being applied in such a way in either case that the areas of the substrate that are separated from each other are not covered by the layer, the layer and substrate being sealingly interconnected at least around the uncovered areas; bringing at least some of the uncovered areas into contact with at least one liquid containing catcher molecules, in such a way that said catcher molecules are able to adhere or bond to the surface of the substrate and/or on the surface of the sensors; removing the non-adhering constituents of the liquid and removing the microstructured layer or parts thereof.

摘要翻译： 本发明涉及一种生产用于检测样品中不同分析物的检测系统的方法，其特征在于以下步骤：提供具有用于化学，光学或电学检测分析物的传感器的平面或基本上平面的基底; 将已经微结构的层施加到基底上或向基底施加连续的层并且对所述层进行微结构化，所述层以这样的方式被施加，即在彼此分离的基底的区域不被层覆盖的情况下 所述层和衬底至少围绕所述未覆盖区域密封地互连; 使至少一些未覆盖区域与至少一种含捕获剂分子的液体接触，使得所述捕获剂分子能够粘附或结合到所述基底的表面和/或所述传感器的表面上; 去除液体的不粘附成分并除去微结构的层或其部分。

公开(公告)号：US07838261B2

公开(公告)日：2010-11-23

申请号：US10432782

申请日：2001-11-26

申请人： Walter Gumbrecht ,  Rainer Hintsche ,  Konrad Mund ,  Manfred Stanzel

发明人： Walter Gumbrecht ,  Rainer Hintsche ,  Konrad Mund ,  Manfred Stanzel

IPC分类号： G01N33/58

CPC分类号： B01J19/0093 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00333 ,  B01J2219/00335 ,  B01J2219/00351 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00644 ,  B01J2219/00659 ,  B01J2219/00783 ,  B01J2219/00828 ,  B01J2219/0086 ,  B01J2219/00867 ,  B01L3/502 ,  B01L3/50853 ,  B01L2200/0642 ,  B01L2200/0673 ,  B01L2300/0819 ,  B01L2300/0829 ,  C40B60/14 ,  G01N27/3277

摘要： A method for biochemical analysis uses a micro-reaction array with at least two reaction chambers for materials which react together chemically or biochemically. The reaction chambers are smaller than 1 μl, the reaction chambers are filled together by throughflow, the chemical or biochemical reactions of the substances retained therein then occurs in the individual isolated reaction chambers, thus preventing an interference between the reactions in the individual reaction chambers and the reaction products remain enclosed in the relevant reaction chambers. In the system the planar array has at least two reaction chambers for substances, whereby the reaction chambers are closed with the goal of preventing an exchange of substances.

摘要翻译： 用于生化分析的方法使用具有至少两个反应室的微反应阵列用于化学或生化反应的材料。 反应室小于1μl，反应室通过通流填充在一起，其中保留的物质的化学或生物化学反应发生在各个分离的反应室中，从而防止各反应室中的反应与 反应产物保持封闭在相关反应室中。 在系统中，平面阵列具有至少两个用于物质的反应室，由此反应室被封闭，目的是防止物质交换。

公开(公告)号：US07367221B2

公开(公告)日：2008-05-06

申请号：US11119298

申请日：2005-04-28

申请人： Rainer Hintsche

发明人： Rainer Hintsche

IPC分类号： G01N11/00 ,  G01N27/403

CPC分类号： G01N27/403

摘要： This invention relates to a sensor for the detection of liquid ingredients with a carrier element (10), a sensor element (1) on the carrier element (10) and a sensor array, which is disposed on the surface of the sensor element and contains one or more electrodes (2), which are in electrical contact (4) with the corresponding deflection surfaces (3), which are also on the surface of the sensor element (1), but otherwise not electrically connected to the carrier element (10). The sensor forms a detection unit together with a detachable cover, which has at least one fluid channel (7) for the feed line, as well as at least one contacting element (5) and which forms together with the sensor at least one hollow space (8) above the surface of the sensor element (1), where there is the surface or part of a surface of at least one electrode (2), but no deflection surface (3), while each contacting element is in such a manner disposed, that it contacts a corresponding deflection surface when the sensor connects with the cover. In specific embodiments, the sensor itself comprises in addition a covering element (6) firmly attached to the sensor, which constitutes at least one filling opening (12) and at least one hollow space (8) above a sensor element's surface (1), where there is the surface or part of a surface of at least one electrode (2), but no deflection surface (3). When connecting this sensor to a contacting element (5), it forms a detection unit, whereby each contacting element is in such a manner disposed, that it can connect to a corresponding deflection surface (3) of the sensor.

摘要翻译： 本发明涉及一种用于检测具有载体元件（10），载体元件（10）上的传感器元件（1）和传感器阵列的液体成分的传感器，该传感器阵列设置在传感器元件的表面上并且包含 一个或多个电极（2），它们与传感器元件（1）的表面上也与传感器元件（1）的表面电接触（4）以及相应的偏转表面（3），但是不电连接到载体元件 ）。 传感器与可拆卸的盖一起形成检测单元，该可拆卸盖具有至少一个用于进料管线的流体通道（7）以及至少一个接触元件（5），并与传感器一起形成至少一个中空空间 （1）的表面之上，其中存在至少一个电极（2）的表面或部分表面，但没有偏转表面（3），而每个接触元件都是这样的 当传感器与盖连接时，它接触相应的偏转表面。 在具体实施例中，传感器本身还包括牢固地附接到传感器的覆盖元件（6），其构成传感器元件表面（1）上方的至少一个填充开口（12）和至少一个中空空间（8） 其中存在至少一个电极（2）的表面或部分表面，但没有偏转表面（3）。 当将该传感器连接到接触元件（5）时，其形成检测单元，由此每个接触元件设置成这样一种方式，即它可以连接到传感器的相应偏转表面（3）。

公开(公告)号：US20050268701A1

公开(公告)日：2005-12-08

申请号：US11119298

申请日：2005-04-28

申请人： Rainer Hintsche

发明人： Rainer Hintsche

IPC分类号： G01N27/403 ,  G01N11/00

CPC分类号： G01N27/403

摘要： This invention regards a sensor for the detection of liquid ingredients with a carrier element (10), a sensor element (1) on the carrier element (10) and a sensor array, which is disposed on the surface of the sensor element and contains one or more electrodes (2), which are in electrical contact (4) with the corresponding deflection surfaces (3), which are also on the surface of the sensor element (1), but otherwise not electrically connected to the carrier element (10). The sensor forms a detection unit together with a detachable cover, which has at least one fluid channel (7) for the feed line, as well as at least one contacting element (5) and which forms together with the sensor at least one hollow space (8) above the surface of the sensor element (1), where there is the surface or part of a surface of at least one electrode (2), but no deflection surface (3), while each contacting element is in such a manner disposed, that it contacts a corresponding deflection surface when the sensor connects with the cover. In specific embodiments, the sensor itself comprises in addition a covering element (6) firmly attached to the sensor, which constitutes at least one filling opening (12) and at least one hollow space (8) above a sensor element's surface (1), where there is the surface or part of a surface of at least one electrode (2), but no deflection surface (3). When connecting this sensor to a contacting element (5), it forms a detection unit, whereby each contacting element is in such a manner disposed, that it can connect to a corresponding deflection surface (3) of the sensor.

摘要翻译： 本发明涉及用于检测具有载体元件（10），载体元件（10）上的传感器元件（1）和传感器阵列的液体成分的传感器，该传感器阵列设置在传感器元件的表面上并且包含一个 或更多的电极（2），其与传感器元件（1）的表面上也具有相应的偏转表面（3）处于电接触（4），但是不与载体元件（10）电连接， 。 传感器与可拆卸的盖一起形成检测单元，该可拆卸盖具有至少一个用于进料管线的流体通道（7）以及至少一个接触元件（5），并与传感器一起形成至少一个中空空间 （1）的表面之上，其中存在至少一个电极（2）的表面或部分表面，但没有偏转表面（3），而每个接触元件都是这样的 当传感器与盖连接时，它接触相应的偏转表面。 在具体实施例中，传感器本身还包括牢固地附接到传感器的覆盖元件（6），其构成传感器元件表面（1）上方的至少一个填充开口（12）和至少一个中空空间（8） 其中存在至少一个电极（2）的表面或部分表面，但没有偏转表面（3）。 当将该传感器连接到接触元件（5）时，其形成检测单元，由此每个接触元件设置成这样一种方式，即它可以连接到传感器的相应偏转表面（3）。

公开(公告)号：US06811037B2

公开(公告)日：2004-11-02

申请号：US09900924

申请日：2001-07-10

申请人： Rainer Hintsche

发明人： Rainer Hintsche

IPC分类号： B01D2946

CPC分类号： G01N27/40

摘要： The invention provides a mechanically stable substrate having at least one through-opening, a perforated membrane which is integral or fluid-tightly connected with the substrate and which extends across the through-opening, and at least one semi-permeable layer which is applied in firmly adhering manner to one or both sides of the membrane, at least in the perforated region thereof, in that the semi-permeable layer or semi-permeable layers is/are secured mechanically in the adjacent perforations and/or by chemical-structural and/or physical adhesion and/or adhesive intermediate layers and/or covalent surface bonding to the adjacent surfaces of the membrane, optionally of the substrate, or of a metallic film optionally additionally applied to one or both sides of the membrane. The invention further provides a process for the production of this sensor- and/or separating element and the use thereof.

摘要翻译： 本发明提供了一种机械稳定的基材，其具有至少一个通孔，穿孔膜与基底整体或流体紧密地连接并延伸穿过通孔，以及至少一个半渗透层 至少在其穿孔区域中牢固地粘附到膜的一侧或两侧，因为半渗透层或半渗透层在相邻穿孔中机械地固定和/或通过化学结构和/ 或物理粘合和/或粘合剂中间层和/或共价表面粘合到膜的任选的基底的相邻表面，或任选另外施加到膜的一侧或两侧的金属膜。 本发明还提供了用于生产该传感器和/或分离元件的方法及其用途。