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1.发明授权Method for preventing chemical crosstalk in enzyme-linked reactions, and associated system 有权防止酶联反应中化学串扰的方法及相关系统公开(公告)号：US07838261B2
公开(公告)日：2010-11-23
申请号：US10432782
申请日：2001-11-26
IPC分类号： G01N33/58
CPC分类号： B01J19/0093 , B01J19/0046 , B01J2219/00317 , B01J2219/00333 , B01J2219/00335 , B01J2219/00351 , B01J2219/00608 , B01J2219/00612 , B01J2219/00621 , B01J2219/00644 , B01J2219/00659 , B01J2219/00783 , B01J2219/00828 , B01J2219/0086 , B01J2219/00867 , B01L3/502 , B01L3/50853 , B01L2200/0642 , B01L2200/0673 , B01L2300/0819 , B01L2300/0829 , C40B60/14 , G01N27/3277
摘要： A method for biochemical analysis uses a micro-reaction array with at least two reaction chambers for materials which react together chemically or biochemically. The reaction chambers are smaller than 1 μl, the reaction chambers are filled together by throughflow, the chemical or biochemical reactions of the substances retained therein then occurs in the individual isolated reaction chambers, thus preventing an interference between the reactions in the individual reaction chambers and the reaction products remain enclosed in the relevant reaction chambers. In the system the planar array has at least two reaction chambers for substances, whereby the reaction chambers are closed with the goal of preventing an exchange of substances.
摘要翻译： 用于生化分析的方法使用具有至少两个反应室的微反应阵列用于化学或生化反应的材料。 反应室小于1μl，反应室通过通流填充在一起，其中保留的物质的化学或生物化学反应发生在各个分离的反应室中，从而防止各反应室中的反应与 反应产物保持封闭在相关反应室中。 在系统中，平面阵列具有至少两个用于物质的反应室，由此反应室被封闭，目的是防止物质交换。
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公开(公告)号：US06881379B1
公开(公告)日：2005-04-19
申请号：US09958807
申请日：2000-04-14
CPC分类号： B82Y30/00 , B01J19/0046 , B01J2219/00317 , B01J2219/00378 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/00635 , B01J2219/00637 , B01J2219/00653 , B01J2219/00659 , B01J2219/00677 , B01J2219/00704 , G01N27/27 , G01N27/3276
摘要： The invention relates to a method for producing a detection system for detecting different analytes in a sample, characterised by the following steps: providing a planar or essentially planar substrate which has sensors for chemically, optically or electrically detecting the analytes; applying an already microstructured layer to the substrate or applying a continuous layer to the substrate and microstructuring said layer, the layer being applied in such a way in either case that the areas of the substrate that are separated from each other are not covered by the layer, the layer and substrate being sealingly interconnected at least around the uncovered areas; bringing at least some of the uncovered areas into contact with at least one liquid containing catcher molecules, in such a way that said catcher molecules are able to adhere or bond to the surface of the substrate and/or on the surface of the sensors; removing the non-adhering constituents of the liquid and removing the microstructured layer or parts thereof.
摘要翻译： 本发明涉及一种生产用于检测样品中不同分析物的检测系统的方法，其特征在于以下步骤：提供具有用于化学，光学或电学检测分析物的传感器的平面或基本上平面的基底; 将已经微结构的层施加到基底上或向基底施加连续的层并且对所述层进行微结构化，所述层以这样的方式被施加，即在彼此分离的基底的区域不被层覆盖的情况下 所述层和衬底至少围绕所述未覆盖区域密封地互连; 使至少一些未覆盖区域与至少一种含捕获剂分子的液体接触，使得所述捕获剂分子能够粘附或结合到所述基底的表面和/或所述传感器的表面上; 去除液体的不粘附成分并除去微结构的层或其部分。
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3.发明授权Method for measuring the concentration or change in concentration of a redox-active substance and corresponding device 有权用于测量氧化还原活性物质浓度或浓度变化的方法及相应的装置公开(公告)号：US08105478B2
公开(公告)日：2012-01-31
申请号：US10587837
申请日：2005-01-26
申请人： Heike Barlag , Walter Gumbrecht , Konrad Mund
发明人： Heike Barlag , Walter Gumbrecht , Konrad Mund
IPC分类号： G01N27/26 , G01N33/53 , G01N33/487
CPC分类号： G01N27/3277 , G01N2333/916 , Y10T436/143333
摘要： In order to follow the change in concentration of a redox-active substance, potential suitable for a reducing process or oxidation process are applied to the working electrode of a measuring device. The potential of the working electrode is pulsed and measuring phases and relaxation phases are alternately produced, the pulse lengths of the measuring phase and relaxation phase being predetermined in a suitable manner. In this manner, a rapid relaxation of the concentration gradient is forced electrochemically so that the measurement can be carried out on simple transducer arrays. The device includes a transducer array in addition to a suitable potentiostat. The transducer array may include a planar metal substrate on which at least one flexible insulator having a firm connection between the metal surface and the insulator surface is located. The array is generated by suitably structuring the substrate.
摘要翻译： 为了跟踪氧化还原活性物质的浓度变化，适用于还原过程或氧化过程的电位被施加到测量装置的工作电极。 工作电极的电位是脉冲的，交替地产生测量相位和弛豫阶段，测量相位和弛豫阶段的脉冲长度以合适的方式被预先确定。 以这种方式，电化学强迫浓缩梯度的快速松弛，使得可以在简单的换能器阵列上进行测量。 除了合适的恒电位仪之外，该装置还包括一个换能器阵列。 换能器阵列可以包括平面金属基底，其上定位有在金属表面和绝缘体表面之间具有牢固连接的至少一个柔性绝缘体。 该阵列通过适当地构造基底而产生。
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4.发明申请METHOD AND SYTEM FOR DETERMINING THE CONCENTRATION OF AN ANALYTE/ENZUME COMPLEX OR OF AN ANALYTE/ENZYME CONJUGATE, ESPECIALLY FOR THE ELECTROCHEMICAL DETECTION OF THE ANALYTE, AND CORRESPONDING MEASURING DEVICE 有权用于确定分析物/酶复合物浓度或分析物/酶合成浓度的方法和系统，特别是用于分析仪的电化学检测和相应的测量装置公开(公告)号：US20100155263A1
公开(公告)日：2010-06-24
申请号：US11989938
申请日：2006-07-31
申请人： Heike Barlag , Walter Gumbrecht , Konrad Mund
发明人： Heike Barlag , Walter Gumbrecht , Konrad Mund
IPC分类号： G01N33/543
CPC分类号： C12Q1/001 , G01N27/3271 , Y10S435/817
摘要： A method follow the change in concentration of a redox-active substance, whereby suitable potentials for a reduction process or an oxidation process are applied to the working electrode of a measuring device. The potential of the working electrode is pulsed and measuring phases and relaxation phases are alternately produced, the pulse lengths of measuring phase and relaxation phase being determined in a suitable manner. A rapid relaxation of the concentration gradient is electrochemically forced so that the measurement can be carried out on simple transducer arrays. A device for carrying out the method includes a transducer array in addition to a suitable potentiostat. The transducer array can be formed of a planar metal substrate on which at least one flexible insulator is disposed, the metal surface and the insulator surface being firmly linked. The transducer array can also be formed of silicone-based CMOS structures.
摘要翻译： 一种遵循氧化还原活性物质浓度变化的方法，由此将适当的还原过程或氧化过程的电位施加到测量装置的工作电极上。 工作电极的电位是脉冲的，并且交替地产生测量相位和松弛相位，测量相位和弛豫阶段的脉冲长度以适当的方式确定。 浓度梯度的快速弛豫是电化学强制的，以便可以在简单的换能器阵列上进行测量。 用于执行该方法的装置包括除合适的恒电位仪之外的换能器阵列。 换能器阵列可以由其上设置有至少一个柔性绝缘体的平面金属基板形成，金属表面和绝缘体表面牢固地连接。 换能器阵列也可以由硅基CMOS结构形成。
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5.发明申请Method for measuring the concentration or change in concentration of a redox-active substance and corresponding device 有权用于测量氧化还原活性物质浓度或浓度变化的方法及相应的装置公开(公告)号：US20080099347A1
公开(公告)日：2008-05-01
申请号：US10587837
申请日：2005-01-26
申请人： Heike Barlag , Walter Gumbrecht , Konrad Mund
发明人： Heike Barlag , Walter Gumbrecht , Konrad Mund
CPC分类号： G01N27/3277 , G01N2333/916 , Y10T436/143333
摘要： In order to follow the change in concentration of a redox-active substance, potential suitable for a reducing process or oxidation process are applied to the working electrode of a measuring device. The potential of the working electrode is pulsed and measuring phases and relaxation phases are alternately produced, the pulse lengths of the measuring phase and relaxation phase being predetermined in a suitable manner. In this manner, a rapid relaxation of the concentration gradient is forced electrochemically so that the measurement can be carried out on simple transducer arrays. The device includes a transducer array in addition to a suitable potentiostat. The transducer array may include a planar metal substrate on which at least one flexible insulator having a firm connection between the metal surface and the insulator surface is located. The array is generated by suitably structuring the substrate.
摘要翻译： 为了跟踪氧化还原活性物质的浓度变化，适用于还原过程或氧化过程的电位被施加到测量装置的工作电极。 工作电极的电位是脉冲的，交替地产生测量相位和弛豫阶段，测量相位和弛豫阶段的脉冲长度以合适的方式被预先确定。 以这种方式，电化学强迫浓缩梯度的快速松弛，使得可以在简单的换能器阵列上进行测量。 除了合适的恒电位仪之外，该装置还包括一个换能器阵列。 换能器阵列可以包括平面金属基底，其上定位有在金属表面和绝缘体表面之间具有牢固连接的至少一个柔性绝缘体。 该阵列通过适当地构造基底而产生。
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公开(公告)号：US20060216813A1
公开(公告)日：2006-09-28
申请号：US10539817
申请日：2003-12-15
申请人： Walter Gumbrecht , Konrad Mund , Meinrad Schienle , Roland Thewes
发明人： Walter Gumbrecht , Konrad Mund , Meinrad Schienle , Roland Thewes
IPC分类号： C12M1/34
CPC分类号： G01N33/5438 , C12Q1/6825
摘要： A DNA chip includes a carrier and a microarray of spots containing immobilised catcher molecules which are arranged on the carrier. Each spot contains a microelectrode system for the impedance spectroscopic detection of binding events occurring between the catcher molecules and target molecules of an analyte solution applied to the spots. The microelectrode system has a pair of polarisation electrodes in order to produce an alternating electromagnetic field and a pair of sensor electrodes for measuring a voltage drop in the analyte.
摘要翻译： DNA芯片包括布置在载体上的载体和含有固定捕获剂分子的斑点的微阵列。 每个斑点包含用于阻抗光谱检测在施加到斑点的分析物溶液的捕获剂分子和靶分子之间发生的结合事件的微电极系统。 微电极系统具有一对极化电极以产生交变电磁场和一对传感器电极，用于测量分析物中的电压降。
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7.发明授权Method and system for determining the concentration of an analyte/enzyme complex or of an analyte/enzyme conjugate, especially for the electrochemical detection of the analyte, and corresponding measuring device 有权用于确定分析物/酶复合物或分析物/酶缀合物的浓度的方法和系统，特别是用于分析物的电化学检测，以及对应的测量装置公开(公告)号：US08128803B2
公开(公告)日：2012-03-06
申请号：US11989938
申请日：2006-07-31
申请人： Heike Barlag , Walter Gumbrecht , Konrad Mund
发明人： Heike Barlag , Walter Gumbrecht , Konrad Mund
IPC分类号： G01N33/543 , G01N27/26 , G01N27/49
CPC分类号： C12Q1/001 , G01N27/3271 , Y10S435/817
摘要： A method follow the change in concentration of a redox-active substance, whereby suitable potentials for a reduction process or an oxidation process are applied to the working electrode of a measuring device. The potential of the working electrode is pulsed and measuring phases and relaxation phases are alternately produced, the pulse lengths of measuring phase and relaxation phase being determined in a suitable manner. A rapid relaxation of the concentration gradient is electrochemically forced so that the measurement can be carried out on simple transducer arrays. A device for carrying out the method includes a transducer array in addition to a suitable potentiostat. The transducer array can be formed of a planar metal substrate on which at least one flexible insulator is disposed, the metal surface and the insulator surface being firmly linked. The transducer array can also be formed of silicone-based CMOS structures.
摘要翻译： 一种遵循氧化还原活性物质浓度变化的方法，由此将适当的还原过程或氧化过程的电位施加到测量装置的工作电极上。 工作电极的电位是脉冲的，并且交替地产生测量相位和松弛相位，测量相位和弛豫阶段的脉冲长度以适当的方式确定。 浓度梯度的快速弛豫是电化学强制的，以便可以在简单的换能器阵列上进行测量。 用于执行该方法的装置包括除合适的恒电位仪之外的换能器阵列。 换能器阵列可以由其上设置有至少一个柔性绝缘体的平面金属基板形成，金属表面和绝缘体表面牢固地连接。 换能器阵列也可以由硅基CMOS结构形成。
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公开(公告)号：US07572624B2
公开(公告)日：2009-08-11
申请号：US10539817
申请日：2003-12-15
申请人： Walter Gumbrecht , Konrad Mund , Meinrad Schienle , Roland Thewes
发明人： Walter Gumbrecht , Konrad Mund , Meinrad Schienle , Roland Thewes
CPC分类号： G01N33/5438 , C12Q1/6825
摘要： A DNA chip includes a carrier and a microarray of spots containing immobilised catcher molecules which are arranged on the carrier. Each spot contains a microelectrode system for the impedance spectroscopic detection of binding events occurring between the catcher molecules and target molecules of an analyte solution applied to the spots. The microelectrode system has a pair of polarisation electrodes in order to produce an alternating electromagnetic field and a pair of sensor electrodes for measuring a voltage drop in the analyte.
摘要翻译： DNA芯片包括布置在载体上的载体和含有固定捕获剂分子的斑点的微阵列。 每个斑点包含用于阻抗光谱检测在施加到斑点的分析物溶液的捕获剂分子和靶分子之间发生的结合事件的微电极系统。 微电极系统具有一对极化电极以产生交变电磁场和一对传感器电极，用于测量分析物中的电压降。
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公开(公告)号：US09995703B2
公开(公告)日：2018-06-12
申请号：US13262578
申请日：2010-03-02
IPC分类号： C40B60/00 , C40B60/12 , G01N27/327 , G01N33/543 , C40B30/04 , C40B50/18
CPC分类号： G01N27/3277 , G01N33/5438
摘要： A device for detecting chemical or biochemical substances in fluids for use in an electrochemical camera. The device includes a first carrier having a sensor array with a plurality of electrochemical sensors. A second carrier includes a porous layer having at least one functional region, in which specifically binding capturing molecules are immobilized. The at least one functional region is arranged directly adjacent to at least one non-functionalized region. Assigned to the at least one functional region and the at least one non-functionalized region are several sensors of the sensor array, for use as the electrochemical camera.
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公开(公告)号：US08975017B2
公开(公告)日：2015-03-10
申请号：US12734934
申请日：2008-11-03
CPC分类号： C12Q1/6806 , C12Q1/6825 , C12Q1/6834 , C12Q1/6837 , Y10T436/143333 , C12Q2527/107 , C12Q2537/125 , C12Q2565/543
摘要： A process concentrates nucleic acid molecules to be detected of a sample on a surface, with capture molecules that specifically bind the nucleic acid molecules. A support material has a capture probe that can specifically be linked to the nucleic acid molecules to be detected to give complexes. The support material and the nucleic acid molecules of the sample are incubated to form the complexes. The complexes are moved to the surface. At least one portion of the complexes becomes bound to the capture molecules via the capture probe.
摘要翻译： 一种方法将待检测的样品的核酸分子浓缩在表面上，其中捕获分子特异性结合核酸分子。 载体材料具有捕获探针，其可以特异性地连接到待检测的核酸分子以得到复合物。 将样品的载体材料和核酸分子孵育以形成复合物。 络合物移动到表面。 复合物的至少一部分经由捕获探针与捕获分子结合。
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