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公开(公告)号:US5011795A
公开(公告)日:1991-04-30
申请号:US499201
申请日:1990-03-22
申请人: Arthur D. Levinson , Diane Pennica , William J. Kohr , Gordon A. Vehar , David V. Goeddel , Elizabeth M. Yelverton , Christian C. Simonsen
发明人: Arthur D. Levinson , Diane Pennica , William J. Kohr , Gordon A. Vehar , David V. Goeddel , Elizabeth M. Yelverton , Christian C. Simonsen
CPC分类号: C12N9/0028 , C12N15/85 , C12N9/6459 , C12Y304/21069 , C12N2800/108 , C12N2840/20
摘要: A method for producing tissue plasminogen activator (t-PA) in eukaryotic host cells is disclosed. Enhanced levels of t-PA production are obtained by co-amplification of the t-PA gene through treatment of cultures transformed with mutant or wild type DHFR with methotrexate.
摘要翻译: 公开了一种在真核宿主细胞中产生组织纤溶酶原激活物(t-PA)的方法。 通过用甲氨蝶呤处理用突变体或野生型DHFR转化的培养物共同扩增t-PA基因,获得增强的t-PA产生水平。
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公开(公告)号:US06261837B1
公开(公告)日:2001-07-17
申请号:US09105412
申请日:1998-06-26
申请人: Arthur D. Levinson , Diane Pennica , William J. Kohr , Gordon A. Vehar , David V. Goeddel , Elizabeth M. Yelverton , Christian C. Simonsen
发明人: Arthur D. Levinson , Diane Pennica , William J. Kohr , Gordon A. Vehar , David V. Goeddel , Elizabeth M. Yelverton , Christian C. Simonsen
IPC分类号: C12N516
CPC分类号: C12N9/0028 , C12N9/6459 , C12N15/85 , C12N2800/108 , C12N2830/00 , C12N2830/55 , C12Y304/21069
摘要: A method for producing tissue plasminogen activator (t-PA) in eukaryotic host cells is disclosed. Enhanced levels of t-PA production are obtained by co-amplification of the t-PA gene through treatment of cultures transformed with mutant or wild type DHFR with methotrexate.
摘要翻译: 公开了一种在真核宿主细胞中产生组织纤溶酶原激活物(t-PA)的方法。 通过用甲氨蝶呤处理用突变体或野生型DHFR转化的培养物共同扩增t-PA基因,获得增强的t-PA产生水平。
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公开(公告)号:US5424198A
公开(公告)日:1995-06-13
申请号:US162354
申请日:1993-12-03
申请人: Arthur D. Levinson , Diane Pennica , William J. Kohr , Gordon A. Vehar , David V. Goeddel , Elizabeth M. Yelverton , Christian C. Simonsen
发明人: Arthur D. Levinson , Diane Pennica , William J. Kohr , Gordon A. Vehar , David V. Goeddel , Elizabeth M. Yelverton , Christian C. Simonsen
CPC分类号: C12N9/0028 , C12N15/85 , C12N9/6459 , C12Y304/21069 , C12N2800/108 , C12N2830/00 , C12N2830/55
摘要: A method for producing tissue plasminogen activator (t-PA)in eukaroytic host cells is disclosed. Enhanced levels of t-PA production are obtained by co-amplification of the t-PA gene through treatment of cultures transformed with mutant or wild-type DHFR with methotrexate.
摘要翻译: 公开了一种用于在真核细胞宿主细胞中产生组织纤溶酶原激活物(t-PA)的方法。 通过用甲氨蝶呤处理用突变体或野生型DHFR转化的培养物共同扩增t-PA基因,获得增强的t-PA产生水平。
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公开(公告)号:US5268291A
公开(公告)日:1993-12-07
申请号:US663103
申请日:1991-02-28
申请人: Arthur D. Levinson , Diane Pennica , William J. Kohr , Gordon A. Vehar , David V. Goeddel , Christian C. Simonsen
发明人: Arthur D. Levinson , Diane Pennica , William J. Kohr , Gordon A. Vehar , David V. Goeddel , Christian C. Simonsen
CPC分类号: C12N9/0028 , C12N15/85 , C12N9/6459 , C12Y304/21069 , C12N2800/108 , C12N2830/00 , C12N2830/55
摘要: A method for producing tissue plasminogen activator (t-PA) in eukaryotic host cells is disclosed. Enhanced levels of t-PA production are obtained by co-amplification of the t-PA gene through treatment of cultures transformed with mutant or wild-type DHFR with methotrexate.
摘要翻译: 公开了一种在真核宿主细胞中产生组织纤溶酶原激活物(t-PA)的方法。 通过用甲氨蝶呤处理用突变体或野生型DHFR转化的培养物共同扩增t-PA基因,获得增强的t-PA产生水平。
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公开(公告)号:US4713339A
公开(公告)日:1987-12-15
申请号:US459152
申请日:1983-01-19
IPC分类号: C12N5/10 , C07H21/04 , C07K14/00 , C07K14/02 , C12N5/00 , C12N15/00 , C12N15/09 , C12N15/67 , C12N15/85 , C12P21/00 , C12Q1/04 , C12R1/91 , C12N1/00 , C12N5/02 , C12P19/34 , C12P21/02 , C12P21/04
CPC分类号: C07K14/005 , C12N15/67 , C12N15/85 , C12N2730/10122 , C12N2840/20
摘要: A polycistronic construction of xenogeneic sequences expressible in eukaryotic cells is described. DHFR encoding sequences under control of the same promoter with another foreign gene can be amplified with methotrexate, and thus cause coamplification of the foreign gene.
摘要翻译: 描述了在真核细胞中可表达的异源序列的多顺反子构建。 用与其他外来基因相同启动子控制的DHFR编码序列可以用甲氨蝶呤进行扩增,从而引起外源基因的共扩增。
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公开(公告)号:USH2181H1
公开(公告)日:2007-02-06
申请号:US07663244
申请日:1991-02-28
申请人: Arthur D. Levinson , Diane Pennica , William J. Kohr , Gordon A. Vehar , David V. Goeddel , Elizabeth M. Yelverton , Christian C. Simonsen
发明人: Arthur D. Levinson , Diane Pennica , William J. Kohr , Gordon A. Vehar , David V. Goeddel , Elizabeth M. Yelverton , Christian C. Simonsen
IPC分类号: C12N15/09
摘要: A method for producing tissue plasminogen activator (t-PA) in eukaryotic host cells is disclosed. Enhanced levels of t-PA production are obtained by co-amplification of the t-PA gene through treatment of cultures transformed with mutant or wild-type DHFR with methotrexate.
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公开(公告)号:US5849574A
公开(公告)日:1998-12-15
申请号:US450874
申请日:1995-05-26
申请人: Arthur D. Levinson , Diane Pennica , William J. Kohr , Gordon A. Vehar , David V. Goeddel , Elizabeth M. Yelverton , Christian C. Simonsen
发明人: Arthur D. Levinson , Diane Pennica , William J. Kohr , Gordon A. Vehar , David V. Goeddel , Elizabeth M. Yelverton , Christian C. Simonsen
CPC分类号: C12N9/0028 , C12N15/85 , C12N9/6459 , C12Y304/21069 , C12N2800/108 , C12N2830/00 , C12N2830/55
摘要: A method for producing tissue plasminogen activator (t-PA) in eukaryotic host cells is disclosed. Enhanced levels of t-PA production are obtained by co-amplification of the t-PA gene through treatment of cultures transformed with mutant or wild type DHFR with methotrexate.
摘要翻译: 公开了一种在真核宿主细胞中产生组织纤溶酶原激活物(t-PA)的方法。 通过用甲氨蝶呤处理用突变体或野生型DHFR转化的培养物共同扩增t-PA基因,获得增强的t-PA产生水平。
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公开(公告)号:US5843636A
公开(公告)日:1998-12-01
申请号:US407410
申请日:1995-03-17
IPC分类号: A61K39/00 , A61K38/00 , A61K39/29 , C07K14/00 , C07K14/02 , C07K14/10 , C07K14/18 , C12N1/21 , C12N15/09 , C12N15/51 , C12P21/02 , C12Q1/70 , C12R1/19 , G01N33/15 , G01N33/50 , G01N33/53 , G01N33/576 , C07H17/00 , C12P21/06
CPC分类号: C07K14/005 , A61K38/00 , C12N2770/24222 , Y10S530/826
摘要: Peptide antigens which are immunoreactive with sera from individuals infected with hepatitis C virus (HCV) are disclosed. Several of the antigens are immunologically reactive with antibodies present in individuals identified as having chronic and acute HCV infection. The antigens are useful in diagnostic methods for detecting HCV infection in humans. Also disclosed are corresponding genomic-fragment clones containing polynucleotides encoding the open reading frame sequences for the antigenic peptides.
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公开(公告)号:US5843639A
公开(公告)日:1998-12-01
申请号:US485500
申请日:1995-06-07
IPC分类号: A61K39/00 , A61K38/00 , A61K39/29 , C07K14/00 , C07K14/02 , C07K14/10 , C07K14/18 , C12N1/21 , C12N15/09 , C12N15/51 , C12P21/02 , C12Q1/70 , C12R1/19 , G01N33/15 , G01N33/50 , G01N33/53 , G01N33/576
CPC分类号: C07K14/005 , A61K38/00 , C12N2770/24222 , Y10S530/826
摘要: Peptide antigens which are immunoreactive with sera from individuals infected with hepatitis C virus (HCV) are disclosed. Several of the antigens are immunologically reactive with antibodies present in individuals identified as having chronic and acute HCV infection. The antigens are useful in diagnostic methods for detecting HCV infection in humans. Also disclosed are corresponding genomic-fragment clones containing polynucleotides encoding the open reading frame sequences for the antigenic peptides.
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