公开(公告)号：US20110129816A1

公开(公告)日：2011-06-02

申请号：US12996340

申请日：2009-06-05

申请人： Atsushi Muraguchi ,  Hiroyuki Kishi ,  Tatsuhiko Ozawa ,  Mamoru Ikemoto ,  Hitoshi Ono ,  Kazuki Morita ,  Koji Uzawa

发明人： Atsushi Muraguchi ,  Hiroyuki Kishi ,  Tatsuhiko Ozawa ,  Mamoru Ikemoto ,  Hitoshi Ono ,  Kazuki Morita ,  Koji Uzawa

IPC分类号： C12Q1/70 ,  C12M1/34 ,  C07K16/10

CPC分类号： C07K16/1018 ,  C07K2317/21 ,  C07K2317/33 ,  C07K2319/61 ,  G01N33/558 ,  G01N33/56983 ,  G01N2333/11

摘要： A device for detecting or quantifying influenza viruses in a sample, which comprises a detection region having a human anti-influenza virus nucleoprotein antibody immobilized onto a support, a sample supply region, and a sample-migrating region; and a kit for detecting or quantifying influenza viruses, which comprises a solid phase in which a human anti-influenza virus nucleoprotein antibody is fixed onto a carrier.

摘要翻译： 用于检测或定量样品中的流感病毒的装置，其包含检测区域，其具有固定在载体上的人抗流感病毒核蛋白抗体，样品供应区域和样品迁移区域; 以及用于检测或定量流感病毒的试剂盒，其包含将抗人流感病毒核蛋白抗体固定在载体上的固相。

公开(公告)号：US20060134704A1

公开(公告)日：2006-06-22

申请号：US10534800

申请日：2003-09-30

申请人： Atsushi Muraguchi ,  Hiroyuki Kishi ,  Eiichi Tamiya ,  Masayasu Suzuki

发明人： Atsushi Muraguchi ,  Hiroyuki Kishi ,  Eiichi Tamiya ,  Masayasu Suzuki

IPC分类号： G01N33/567 ,  C12M1/34 ,  G01N33/53

CPC分类号： C07K16/082 ,  B01J2219/00317 ,  B01J2219/00743 ,  C07K2317/21 ,  C40B60/14 ,  G01N33/5302 ,  G01N33/54366 ,  G01N33/56972

摘要： A microwell array chip that has multiple microwells and is employed to contain a single lymphocyte specimen in each microwell and detect antigen-specific lymphocytes in single units; wherein the microwell array chip is of a shape and of dimensions where only one lymphocyte is contained in each microwell. A method of detecting antigen-specific lymphocytes comprising the steps of adding antigen to each microwell in the above microwell array chip, stimulating the lymphocyte specimen, and detecting lymphocyte specimens reacting with the antigen.

公开(公告)号：US09310362B2

公开(公告)日：2016-04-12

申请号：US13088582

申请日：2011-04-18

申请人： Atsushi Muraguchi ,  Hiroyuki Kishi ,  Yoshiharu Tokimitsu ,  Sachiko Kondo ,  Tsutomu Obata ,  Satoshi Fujiki ,  Yoshiyuki Yokoyama ,  Hirofumi Nabesawa ,  Sotohiro Takabayashi ,  Katsumi Tanino

发明人： Atsushi Muraguchi ,  Hiroyuki Kishi ,  Yoshiharu Tokimitsu ,  Sachiko Kondo ,  Tsutomu Obata ,  Satoshi Fujiki ,  Yoshiyuki Yokoyama ,  Hirofumi Nabesawa ,  Sotohiro Takabayashi ,  Katsumi Tanino

IPC分类号： C12N5/16 ,  C12N5/00 ,  G01N33/543 ,  B01J19/00 ,  A61K35/12 ,  A61K48/00

CPC分类号： G01N33/54366 ,  A61K35/12 ,  A61K48/00 ,  B01J19/0046 ,  B01J2219/00659 ,  C12M23/12 ,  G06F19/00

摘要： A microwell array chip made of silicon and having multiple microwells where each microwell is used to store a single specimen organic cell. The microwell of the array chip is of a size and shape to hold just one organic cell and the interior surface of the microwells are coated with a fluorocarbon film.

摘要翻译： 由硅制成并具有多个微孔的微孔阵列芯片，其中每个微孔用于存储单个样品有机电池。 阵列芯片的微孔具有仅容纳一个有机电池的尺寸和形状，并且微孔的内表面涂覆有氟碳膜。

公开(公告)号：US08664003B2

公开(公告)日：2014-03-04

申请号：US13569291

申请日：2012-08-08

申请人： Eiichi Tamiya ,  Yoshiyuki Yokoyama ,  Satoshi Fujiki ,  Katsumi Tanino ,  Atsushi Muraguchi ,  Hiroyuki Kishi ,  Yoshiharu Tokimitsu ,  Shohei Yamamura

发明人： Eiichi Tamiya ,  Yoshiyuki Yokoyama ,  Satoshi Fujiki ,  Katsumi Tanino ,  Atsushi Muraguchi ,  Hiroyuki Kishi ,  Yoshiharu Tokimitsu ,  Shohei Yamamura

IPC分类号： C12Q1/24

CPC分类号： C12N5/0068 ,  C12N2533/12 ,  C12N2535/10 ,  C12N2537/10 ,  C12N2539/10 ,  Y10T428/24273 ,  Y10T436/25 ,  Y10T436/25375

摘要： A chip useful for treating cells and the like which has a mechanism and a structure wherein the size of a hole pattern is arbitrarily changed so that cells can easily move in and get out from the hole in scattering or collecting cells but can hardly get out from the hole during washing or antigen-stimulation. The chip comprises a crosslinked product of a temperature-responsive polymer as a constituting member and being provided with a film having a hole pattern on the surface of a baseboard. A method of producing the chip comprises applying a composition containing a crosslinkable temperature-responsive polymer on the surface of a baseboard to thereby form a coating film, crosslinking the coating film to thereby form the crosslinked product as described above and then forming a hole pattern on the coating film of the crosslinked product.

摘要翻译： 用于处理细胞等的芯片，其具有机构和结构，其中孔图案的尺寸任意改变，使得细胞可以容易地在散射或收集细胞中从孔中移出并离开，但几乎不能从 洗涤期间的孔或抗原刺激。 芯片包括作为构成部件的温度敏感性聚合物的交联体，并且在基板的表面上设置有具有孔图案的膜。 制造该芯片的方法包括在基板的表面上涂布含有交联性温度敏感性聚合物的组合物，从而形成涂膜，使涂膜交联从而形成如上所述的交联产物，然后在 交联产物的涂膜。

公开(公告)号：US20140162320A1

公开(公告)日：2014-06-12

申请号：US14008568

申请日：2012-03-27

申请人： Masae Horii ,  Hiroyuki Kishi ,  Eiji Kobayashi ,  Tatsuhikio Ozawa ,  Atsushi Muraguchi

发明人： Masae Horii ,  Hiroyuki Kishi ,  Eiji Kobayashi ,  Tatsuhikio Ozawa ,  Atsushi Muraguchi

IPC分类号： C12N15/63

CPC分类号： C12N15/64 ,  C12N15/63 ,  C12N15/86 ,  C12N15/867 ,  C12N2740/10043 ,  C12N2820/55 ,  C12N2820/60 ,  C12N2820/704 ,  C12N2820/706

摘要： The purpose of the invention is to provide means with which it is possible to efficiently select a vector to which a foreign gene has been introduced when a foreign gene is to be introduced by homologous recombination to a vector having multiple sequences homologous with one another. The vector comprises, in succession, a replication origin, a sequence A, a marker gene X, two sequences C and D for introducing a foreign gene by homologous recombination, and a sequence B homologous with sequence A. The two sequences C and D are directly or indirectly adjacent to one another. The vector is used for introducing a foreign gene between the two adjacent sequences C and D.

摘要翻译： 本发明的目的是提供一种方法，当通过同源重组将外源基因导入具有多个彼此同源的序列的载体时，可以有效地选择引入外源基因的载体。 载体依次包含复制起点，序列A，标记基因X，用于通过同源重组引入外源基因的两个序列C和D以及与序列A同源的序列B.两个序列C和D分别为 直接或间接相邻。 该载体用于在两个相邻序列C和D之间引入外源基因。

公开(公告)号：US20080014631A1

公开(公告)日：2008-01-17

申请号：US10573289

申请日：2004-09-22

申请人： Atsushi Muraguchi ,  Hiroyuki Kishi ,  Yoshiharu Tokimitsu ,  Sachiko Kondo ,  Tsutomu Obata ,  Satoshi Fujiki ,  Yoshiyuki Yokoyama ,  Hirofumi Nabesawa ,  Sotohiro Takabayashi ,  Katsumi Tanino

发明人： Atsushi Muraguchi ,  Hiroyuki Kishi ,  Yoshiharu Tokimitsu ,  Sachiko Kondo ,  Tsutomu Obata ,  Satoshi Fujiki ,  Yoshiyuki Yokoyama ,  Hirofumi Nabesawa ,  Sotohiro Takabayashi ,  Katsumi Tanino

IPC分类号： C12M1/34

CPC分类号： G01N33/54366 ,  A61K35/12 ,  A61K48/00 ,  B01J19/0046 ,  B01J2219/00659 ,  C12M23/12

摘要： A microwell array chip having multiple microwells on a principal surface of a substrate, said microwells being of a shape and size permitting the storage of only a single organic cell in each microwell, wherein microwell markers are present on the same substrate surface as the openings of the microwells. A microwell array chip having multiple microwells on a principal surface of a substrate, said microwells being of a shape and size permitting the storage of only a single organic cell in each microwell, wherein protrusions are present in the openings of said microwells so as to narrow said openings. A method for manufacturing the microwell array chip. The method comprises the steps of: forming a film on at least one principal surface of a substrate; applying a resist coating on the film that has been formed; exposing the resist surface through a mask having a microwell pattern and removing uncured portions of resist; etching the exposed portions of said film and substrate to fabricate wells in the form of a microwell array; and removing the resist. A microwell array chip made of silicon and having multiple microwells, each microwell being used to store a single specimen organic cell, wherein each microwell is of a size and shape holding just one organic cell.

摘要翻译： 一种微孔阵列芯片，其在基底的主表面上具有多个微孔，所述微孔具有允许在每个微孔中仅存储单个有机细胞的形状和尺寸，其中微孔标记物存在于与基底表面的开口相同的基底表面上 微孔。 一种微孔阵列芯片，其在基底的主表面上具有多个微孔，所述微孔具有允许在每个微孔中仅存储单个有机细胞的形状和尺寸，其中突起存在于所述微孔的开口中以使其变窄 说开口 一种微孔阵列芯片的制造方法。 该方法包括以下步骤：在衬底的至少一个主表面上形成膜; 在已经形成的膜上施加抗蚀剂涂层; 通过具有微孔图案的掩模曝光抗蚀剂表面并除去未固化的抗蚀剂部分; 蚀刻所述膜和基底的暴露部分以制造微孔阵列形式的孔; 并除去抗蚀剂。 由硅制成并具有多个微孔的微孔阵列芯片，每个微孔用于存储单个样品有机电池，其中每个微孔具有仅具有一个有机电池的尺寸和形状。

公开(公告)号：US09494575B2

公开(公告)日：2016-11-15

申请号：US12671690

申请日：2008-08-01

申请人： Aishun Jin ,  Hiroyuki Kishi ,  Atsushi Muraguchi ,  Tsutomu Obata

发明人： Aishun Jin ,  Hiroyuki Kishi ,  Atsushi Muraguchi ,  Tsutomu Obata

IPC分类号： C40B30/04 ,  C12N5/0781 ,  C40B60/12 ,  G01N33/50 ,  C12N5/10 ,  C12N5/071 ,  C12N5/16

CPC分类号： G01N33/54366 ,  G01N33/5047 ,  G01N33/566 ,  G01N33/6863 ,  G01N2333/52 ,  G01N2333/715

摘要： Provided are a method and means permitting the simultaneous measurement of the reactive properties of more than 10,000 of antigen-stimulated lymphocytes being held on a chip. A microwell array comprises multiple wells capturing single cells and a coating layer on a principal surface around the wells containing a substance capable of binding to a substance produced by the cells in the wells. A method for screening a target cell comprises: causing specimen cells and a cell culture broth to be contained in the wells of the microwell array; culturing the cells in a state permitting the diffusion of substances from the wells into the coating layer; feeding a label substance binding specifically to a substance produced by a target cell onto the coating layer; and detecting the substance produced by the target cell by the label substance binding around the well to specify the target cell.

摘要翻译： 提供了允许同时测量保持在芯片上的超过10,000个抗原刺激的淋巴细胞的反应性质的方法和手段。 微孔阵列包括捕获单个细胞的多个孔和围绕孔的主表面上的包覆层，其包含能够结合孔中由细胞产生的物质的物质。 用于筛选靶细胞的方法包括：使样品细胞和细胞培养液包含在微孔阵列的孔中; 以允许物质从孔扩散到涂层中的状态培养细胞; 将与靶细胞产生的物质特异性结合的标签物质进料到涂层上; 以及通过在井附近结合的标记物质来检测由靶细胞产生的物质以指定靶细胞。

公开(公告)号：US08445193B2

公开(公告)日：2013-05-21

申请号：US12337481

申请日：2008-12-17

申请人： Atsushi Muraguchi ,  Hiroyuki Kishi ,  Eiichi Tamiya ,  Masayasu Suzuki

发明人： Atsushi Muraguchi ,  Hiroyuki Kishi ,  Eiichi Tamiya ,  Masayasu Suzuki

IPC分类号： G01N33/53 ,  C12N5/06 ,  C12N5/08 ,  C12N5/00

CPC分类号： C07K16/082 ,  B01J2219/00317 ,  B01J2219/00743 ,  C07K2317/21 ,  C40B60/14 ,  G01N33/5302 ,  G01N33/54366 ,  G01N33/56972

摘要： A microwell array chip that has multiple microwells and is employed to contain a single lymphocyte specimen in each microwell and detect antigen-specific lymphocytes in single units; wherein the microwell array chip is of a shape and of dimensions where only one lymphocyte is contained in each microwell. A method of detecting antigen-specific lymphocytes comprising the steps of adding antigen to each microwell in the above microwell array chip, stimulating the lymphocyte specimen, and detecting lymphocyte specimens reacting with the antigen.

摘要翻译： 微孔阵列芯片具有多个微孔，并用于在每个微孔中含有单个淋巴细胞标本，并以单个单位检测抗原特异性淋巴细胞; 其中所述微孔阵列芯片的形状和尺寸在每个微孔中仅包含一个淋巴细胞。 一种检测抗原特异性淋巴细胞的方法，包括以下步骤：向上述微孔阵列芯片中的每个微孔加入抗原，刺激淋巴细胞标本，以及检测与抗原反应的淋巴细胞标本。

公开(公告)号：US20110294678A1

公开(公告)日：2011-12-01

申请号：US12671690

申请日：2008-08-01

申请人： Aishun Jin ,  Hiroyuki Kishi ,  Atsushi Muraguchi ,  Tsutomu Obata

发明人： Aishun Jin ,  Hiroyuki Kishi ,  Atsushi Muraguchi ,  Tsutomu Obata

IPC分类号： C40B30/04 ,  C12N5/0781 ,  C12N5/10 ,  C12N5/071 ,  C12N5/0783 ,  C40B60/12 ,  C12N5/16

CPC分类号： G01N33/54366 ,  G01N33/5047 ,  G01N33/566 ,  G01N33/6863 ,  G01N2333/52 ,  G01N2333/715

摘要： Provided are a method and means permitting the simultaneous measurement of the reactive properties of more than 10,000 of antigen-stimulated lymphocytes being held on a chip and the separate determination of the states of individual cells. A microwell array comprises multiple wells and a coating layer on one of the principal surfaces of a base member, the wells being of a size permitting the entry of only a single cell into each well. A coating layer of a substance capable of binding to a substance produced by the cells contained in the wells is present on the principal surface around the wells. A method of screening for a target cell, comprises: causing specimen cells and a cell culture broth to be contained in the wells of the above microwell array; immersing the coating layer and the wells in the culture broth and culturing the cells in a state permitting the diffusion of substances in the culture broth from the wells into the coating layer; feeding a label substance binding specifically to a substance produced by a target cell present among the specimen cells onto the coating layer; and detecting the substance produced by the target cell that has bound to the substance in the coating layer by the label substance to specify the target cell.

摘要翻译： 提供了允许同时测量保持在芯片上的超过10,000个抗原刺激的淋巴细胞的反应性的方法和手段，以及单独测定各细胞的状态。 微孔阵列包括多个孔和在基底构件的一个主表面上的涂层，所述孔具有允许仅将单个细胞进入每个孔的尺寸。 能够与孔中包含的细胞产生的物质结合的物质的涂层存在于孔周围的主表面上。 筛选靶细胞的方法包括：使样品细胞和细胞培养液包含在上述微孔阵列的孔中; 将涂层和孔浸入培养液中，并使培养液中的物质从孔扩散到涂层中的状态下培养细胞; 将标本物质特异性地与存在于试样细胞中的靶细胞产生的物质结合到涂层上; 以及通过所述标记物质检测由所述靶层细胞产生的与所述涂层中的物质结合的物质，以指定所述靶细胞。

公开(公告)号：US20060078946A1

公开(公告)日：2006-04-13

申请号：US11236553

申请日：2005-09-28

申请人： Atsushi Muraguchi ,  Hiroyuki Kishi ,  Kihachiro Tohbo ,  Minoru Ueno ,  Hiroyoshi Nakazato ,  Eiichi Tamiya ,  Masayasu Suzuki

发明人： Atsushi Muraguchi ,  Hiroyuki Kishi ,  Kihachiro Tohbo ,  Minoru Ueno ,  Hiroyoshi Nakazato ,  Eiichi Tamiya ,  Masayasu Suzuki

IPC分类号： G01N33/567 ,  C12P21/04 ,  G01N33/53

CPC分类号： C07K16/00 ,  C12N5/163

摘要： A method for producing a monoclonal antibody and antigen-specific antibody-producing hybridomas using a single antigen-specific B lymphocyte is provided. The method for producing antigen-specific antibody-producing hybridomas comprises selecting a single B lymphocyte reacting specifically with a certain antigen, an “antigen-specific B lymphocyte”, culturing the antigen-specific B lymphocyte selected, and fusing the cultured antigen-specific B lymphocyte with myeloma cells to obtain the hybridomas. The monoclonal antibody is produced with thus obtained hybridomas.

摘要翻译： 提供了使用单一抗原特异性B淋巴细胞产生单克隆抗体和产生抗原特异性抗体的杂交瘤的方法。 产生产生抗原特异性抗体的杂交瘤的方法包括选择与特定抗原特异性反应的单一B淋巴细胞，“抗原特异性B淋巴细胞”，培养所选择的抗原特异性B淋巴细胞，并将培养的抗原特异性B 淋巴细胞与骨髓瘤细胞以获得杂交瘤。 用如此获得的杂交瘤产生单克隆抗体。