摘要:
An expression system can be regulated by acetate, pH and oxygen, which expression system includes a trans-acting regulator protein and a promoter which can be activated by this protein. Any desired structural genes are maximally expressed under the control of the expression system at an oxygen partial pressure, pO.sub.2, of 0-5% and a pH of 6.0-6.5, and in the presence of acetate at a concentration of 40-60 mM. There is also a process for preparing this expression system, and a process for using this expression system.
摘要:
The alkalophilic bacteria (DSM 5853) possesses a maltopentaose producing amylase. The maltopentaose producing amylase, and its derivatives modified by gene manipulation, can be expressed in E. coli. These amylases facilitate the production of maltopentaose.
摘要:
A maltopentaose producing amylase, and its derivatives modified by gene manipulation, can be expressed in E. col. These amylases facilitate the production of maltopentaose.
摘要:
Process for the preparation of heteroaryl aldehydes or heteroaryl ketones by reaction with heteroarylmethanols with the aid of a mediator and an oxidant, wherein the mediator is selected from the group of the aliphatic, cycloaliphatic, heterocyclic or aromatic NO, and NOH containing compounds.
摘要:
A cyclodextrin glycosyltransferase (CGTase) which, in the conversion of starch or starch-like substrates to CD, produces .gamma.-CD in an increased amount, and whose protein sequence, in the region between amino acid position 180 and amino acid position 240, contains the amino acid sequence (SEQ ID NO: 1), where position 1 of the protein sequence is the beginning of the signal peptide of the CGTase and Xxx denotes a natural amino acid.
摘要翻译:将淀粉或淀粉样底物转化成CD的环糊精糖基转移酶(CGTase)产生增加量的γ-CD,其氨基酸位置180和氨基酸位置240之间的区域中的蛋白质序列含有 氨基酸序列(SEQ ID NO:1),其中蛋白质序列的位置1是CGTase的信号肽的起始点,Xxx表示天然氨基酸。
摘要:
The present invention comprises a signal peptide with a cleavage site to a recombinant protein, wherein the last three amino acids before the cleavage site are alanine-phenylalanine-alanine (AFA).
摘要:
The present invention comprises a signal peptide with a cleavage site to a recombinant protein, wherein the last three amino acids before the cleavage site are alanine-phenylalanine-alanine (AFA).
摘要:
Biological decomposition of compounds containing Si-C bands such as PDMS takes place under substantially anaerobic conditions in the presence of an alternative electron acceptor.
摘要:
A process for the preparation of vinyl, alkynyl or aryl aldehydes or vinyl, alkynyl or aryl ketones by reaction of vinyl-, alkynyl- or aryl- methanols with the aid of a mediator and an oxidant, wherein the mediator is selected from the group of the aliphatic, cycloaliphatic, heterocyclic or aromatic NO or NOH containing compounds.