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    Production of amino acids from sucrose in Corynebacterium glutamicum
    4.
    发明授权
    Production of amino acids from sucrose in Corynebacterium glutamicum 有权
    从谷氨酸棒杆菌中的蔗糖生产氨基酸

    公开(公告)号：US08048649B2

    公开(公告)日：2011-11-01

    申请号：US12239242

    申请日：2008-09-26

    申请人： Thomas P. Binder ,  Paul D. Hanke

    发明人： Thomas P. Binder ,  Paul D. Hanke

    IPC分类号： C12P13/08 ,  C12N9/12 ,  C12N9/22 ,  C12N1/21

    CPC分类号： C12P13/04 ,  C12P13/08 ,  C12R1/15

    摘要： Methods and compositions for increased production of amino acids from C. glutamicum using sucrose as a carbon source are described. In one aspect, increased production of L-lysine from C. glutamicum is accomplished by using a strain having a mutation in the ptsF gene encoding fructose-PTS enzyme that attenuates or blocks fructose import into the cell when such strain is grown on media containing sucrose as a carbon source and production is increased by providing glucose isomerase in the fermentation media. The glucose isomerase may be exogenously added or expressed in the strain and exported into the media. In certain embodiments the media also contain an invertase. In another aspect increased production of L-lysine is accomplished by making a C. glutamicum strain having the ptsF mutation and a second mutation in a fructose exporter function. The dual mutation retains imported fructose in the cell. In certain embodiments, the strain also overexpresses at least one of a glucose isomerase and glucokinase activity in the cell to drive imported fructose toward the pentose phosphate pathway to increase L-lysine production.

    摘要翻译： 描述了使用蔗糖作为碳源来增加谷氨酸棒球菌产生氨基酸的方法和组合物。 一方面，通过使用编码果糖-PTS酶的ptsF基因中的突变的菌株，当将这种菌株在含有蔗糖的培养基上生长时，使果糖进入细胞，从而导致来自谷氨酸棒球菌的L-赖氨酸的生产增加 作为碳源，通过在发酵培养基中提供葡萄糖异构酶来增加产量。 葡萄糖异构酶可以在菌株中外源加入或表达并输出到培养基中。 在某些实施方案中，培养基还含有转化酶。 另一方面，通过制备具有ptsF突变和果糖出口功能中的第二突变的谷氨酸棒杆菌菌株来实现L-赖氨酸的生产增加。 双重突变在细胞中保留了进口的果糖。 在某些实施方案中，菌株也在细胞中过表达葡萄糖异构酶和葡萄糖激酶活性中的至少一种，以驱动进口的果糖向戊糖磷酸途径增加L-赖氨酸生产。

    Method for separating a basic amino acid from fermentation broth
    7.
    发明授权
    Method for separating a basic amino acid from fermentation broth 有权
    从发酵液中分离出碱性氨基酸的方法

    公开(公告)号：US06479700B2

    公开(公告)日：2002-11-12

    申请号：US09808123

    申请日：2001-03-15

    申请人： John Soper ,  Ahmad K. Hilaly ,  Kevin Moore ,  Thomas P. Binder

    发明人： John Soper ,  Ahmad K. Hilaly ,  Kevin Moore ,  Thomas P. Binder

    IPC分类号： C07C22700

    CPC分类号： C07C227/40 ,  C07C229/26

    摘要： This invention relates to a method for separating basic amino acids from fermentation broth comprising contacting the broth with strong acid cation exchange resins that have a low degree of cross-linkage and eluting the amino acid. The method described herein results in higher yield and higher purity of lysine, in addition to higher throughput, as compared to conventional processes of purification of lysine from fermentation broth.

    摘要翻译： 本发明涉及从发酵液中分离碱性氨基酸的方法，包括使肉汤与具有低交联度的氨基酸的强酸性阳离子交换树脂接触。 与来自发酵液的赖氨酸的纯化的常规方法相比，本文所述的方法除了产生更高的产量之外还导致更高的产量和更高的赖氨酸纯度。

    Process for making L-lysine feed supplement
    8.
    发明授权
    Process for making L-lysine feed supplement 失效
    制备L-赖氨酸饲料添加剂的方法

    公开(公告)号：US6017555A

    公开(公告)日：2000-01-25

    申请号：US98948

    申请日：1998-06-17

    申请人： Joseph Michael Stevens ,  Thomas P. Binder

    发明人： Joseph Michael Stevens ,  Thomas P. Binder

    IPC分类号： A23K1/00 ,  A23K1/16 ,  C12P13/08 ,  A23K1/18

    CPC分类号： C12P13/08 ,  A23K20/142 ,  A23K40/10

    摘要： A process produces an L-Lysine feed supplement with a final L-Lysine purity in the range theoretically between about 35% and 80%, measured as a percent of free-base per kg, and more preferably between about 50% and 80% L-Lysine. The process comprises adding a material containing L-Lysine to an L-Lysine fermentation broth or a fraction of an L-Lysine fermentation broth. The added material being an amount which brings a final L-Lysine feed supplement with an L-Lysine purity into a range theoretically between about 35% and 80%, measured as a percent of free-base per kg, and more preferably between about 50% and 80% L-Lysine. The fraction of L-Lysine fermentation broth is obtained by any suitable separating means such as ultrafiltration or centrifugation. The process also comprises a drying step which may involve any suitable drying means such as a spray granulator, spray dryer, tray dryer, drum dryer, rotary dryer, and tunnel dryer.

    摘要翻译： 一种方法产生L-赖氨酸饲料添加物，其最终L-赖氨酸纯度在理论上在约35％至80％的范围内，以游离碱/ kg的百分比测量，更优选约50％至80％L -Lysine。 该方法包括向L-赖氨酸发酵液或L-赖氨酸发酵液的一部分中加入含有L-赖氨酸的物质。 添加的物质是将L-赖氨酸纯度的最终L-赖氨酸饲料添加物在理论上达到约35％至80％的范围内的量，以游离碱/ kg的百分比测量，更优选约50 ％和80％L-赖氨酸。 通过任何合适的分离方法如超滤或离心获得L-赖氨酸发酵液的分数。 该方法还包括干燥步骤，其可以包括任何合适的干燥装置，例如喷雾造粒机，喷雾干燥器，塔盘干燥器，滚筒干燥器，旋转干燥器和隧道式干燥器。

    Process for fractionation of lignocellulosic biomass
    9.
    发明授权
    Process for fractionation of lignocellulosic biomass 失效
    木质纤维素生物质分馏方法

    公开(公告)号：US08637282B2

    公开(公告)日：2014-01-28

    申请号：US13521469

    申请日：2011-01-21

    申请人： Thomas P. Binder ,  Paul D. Bloom ,  Perry H. Doane ,  Chi-Cheng Ma

    发明人： Thomas P. Binder ,  Paul D. Bloom ,  Perry H. Doane ,  Chi-Cheng Ma

    IPC分类号： C12P7/10

    CPC分类号： C12P7/10 ,  A23K10/32 ,  A23K40/10 ,  A23K50/10 ,  C07G1/00 ,  C08H8/00 ,  C12P2203/00 ,  Y02E50/10 ,  Y02E50/16 ,  Y02E50/30 ,  Y02P60/877 ,  Y02T50/678

    摘要： Methods are provided for the efficient fractionation of lignocellulosic biomasses into cellulosic, hemicellulosic and lignin fractions, wherein concentrated organic acid vapors are applied to the biomass at elevated temperatures at the location(s) or near the location(s) where the biomass has been harvested and gathered, to at least partly depolymerize or substantially solubilize the hemicelluloses and lignins in the biomass. The organic acid-treated biomass is in either case then dried and pelletized for extended bulk storage and/or for shipment to a second facility some distance away. The organic acid-treated biomass may be processed into desired chemicals, fuels and/or fuel additives at the local processing site or at a second facility away from the local processing site, or the pelletized material may be used as a ruminant feed locally or at a feedlot some distance removed from the local processing site.

    摘要翻译： 提供了将木质纤维素生物质有效分级成纤维素，半纤维素和木质素级分的方法，其中将浓缩的有机酸蒸气在升高的温度下在生物量已被收获的位置或位置附近施用于生物质 并聚集，至少部分地解聚或基本上溶解生物质中的半纤维素和木质素。 有机酸处理的生物质在任一情况下然后干燥并造粒用于延长的大量储存和/或运送到距离一段距离的第二设施。 有机酸处理的生物质可以在局部加工部位或远离局部加工部位的第二设施处理成所需的化学品，燃料和/或燃料添加剂，或者造粒材料可以在本地或在 一个距离当地加工地点一定距离的饲养场。

    GRAIN MILLING PROCESS
    10.
    发明申请
    GRAIN MILLING PROCESS 审中-公开
    砂磨工艺

    公开(公告)号：US20130102045A1

    公开(公告)日：2013-04-25

    申请号：US13521365

    申请日：2011-01-19

    申请人： Thomas P. Binder

    发明人： Thomas P. Binder

    IPC分类号： C08B30/12

    CPC分类号： C13K13/00 ,  A23V2002/00 ,  C08B30/12 ,  C08L1/02 ,  C08L97/00 ,  C12P7/10 ,  C12P2203/00 ,  C13B10/003 ,  Y02E50/16 ,  A23V2250/02

    摘要： A modification is described of a dry grind process for producing ethanol and other co-products from whole grain, whereby the mash is thermochemically treated by cooking the mash in the presence of an organic acid. The organic acid effectively hydrolyzes both the starch and hemicellulosic components in the milled corn to provide fermentable sugars from both the endosperm and other parts of the kernel, without, however, also producing fermentation-inhibiting levels of other known products of the acid hydrolysis of hemicellulosic materials, such as hydroxymethylfurfural (HMF) and furfural. Further, the organic acid is able to solubilize both the starch and the more recalcitrant hemicelluloses while only partially hydrolyzing the same, so that most of the starch and hemicelluloses are hydrolyzed to oligomers and the amount of chemically labile and reducing sugars is kept sufficiently low as to also not appreciably interfere with the fermentation to ethanol.

    摘要翻译： 描述了用于从全谷物生产乙醇和其它副产物的干式研磨方法的改进，由此通过在有机酸存在下烹饪醪液来对该糊状物进行热化学处理。 有机酸有效地水解碾磨的玉米中的淀粉和半纤维素组分，以提供来自胚乳和其他部分核仁的可发酵糖，而不产生抑制水平的其他已知产物的半纤维素酸水解 材料，如羟甲基糠醛（HMF）和糠醛。 此外，有机酸能够溶解淀粉和更顽固的半纤维素，同时仅使其部分水解，使得大多数淀粉和半纤维素被水解成低聚物，并且化学不稳定和还原糖的量保持足够低，如 也不会明显地干扰乙醇的发酵。