公开(公告)号：US11203018B2

公开(公告)日：2021-12-21

申请号：US16160816

申请日：2018-10-15

Applicant: Berkeley Lights, Inc.

Inventor： Troy A. Lionberger ,  Matthew E. Fowler ,  Phillip J. M. Elms ,  Kevin D. Loutherback ,  Randall D. Lowe, Jr. ,  Jian Gong ,  J. Tanner Nevill ,  Gang F. Wang ,  Gregory G. Lavieu ,  John A. Tenney ,  Aathavan Karunakaran ,  Anupam Singhal ,  I-Jong Lin

IPC: B01L3/00 ,  G01N21/64 ,  B03C5/02 ,  B03C5/00 ,  B01L9/00 ,  G06T7/00 ,  G01N21/05

Abstract: Methods, systems and kits are described herein for detecting the results of an assay. In particular, the methods, systems and devices of the present disclosure rely on a difference between the diffusion rates of a reporter molecule and an analyte of interest in order to quantify an amount of analyte in a microfluidic device. The analyte may be a secreted product of a biological micro-object.

公开(公告)号：US11454629B2

公开(公告)日：2022-09-27

申请号：US16908265

申请日：2020-06-22

Applicant: Berkeley Lights, Inc.

Inventor： Kristin G. Beaumont ,  Peter J. Beemiller ,  Volker L. S. Kurz ,  Gregory G. Lavieu ,  Xiaohua Wang ,  Aathavan Karunakaran

IPC: B01L3/00 ,  G01N33/543 ,  G01N33/545 ,  G01N33/58 ,  G01N33/50

Abstract: In situ-generated microfluidic capture structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. Microfluidic capture structures may be advantageously used for assays performed within the microfluidic environment, providing flexibility in assaying micro-objects such as biological cells. Assay reagents and analytes may be incorporated within the microfluidic capture structures.

公开(公告)号：US11365381B2

公开(公告)日：2022-06-21

申请号：US16920365

申请日：2020-07-02

Applicant: Berkeley Lights, Inc.

Inventor： Randall D. Lowe, Jr. ,  Kristin G. Beaumont ,  Aathavan Karunakaran ,  Natalie C. Marks ,  Jason M. McEwen ,  Mark P. White ,  J. Tanner Nevill ,  Gang F. Wang ,  Andrew W. McFarland ,  Daniele Malleo ,  Keith J. Breinlinger ,  Xiao Guan ,  Kevin T. Chapman

IPC: C12M3/00 ,  B01L3/00 ,  C12M3/06 ,  C12M1/00

Abstract: Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.

公开(公告)号：US20170184583A1

公开(公告)日：2017-06-29

申请号：US15372094

申请日：2016-12-07

Applicant: Berkeley Lights, Inc.

Inventor： Kristin G. Beaumont ,  Peter J. Beemiller ,  Volker L.S. Kurz ,  Gregory G. Lavieu ,  Xiaohua Wang ,  Aathavan Karunakaran

IPC: G01N33/543 ,  G01N33/58 ,  G01N33/545

Abstract: In situ-generated microfluidic capture structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. Microfluidic capture structures may be advantageously used for assays performed within the microfluidic environment, providing flexibility in assaying micro-objects such as biological cells. Assay reagents and analytes may be incorporated within the microfluidic capture structures.

公开(公告)号：US10705082B2

公开(公告)日：2020-07-07

申请号：US15372094

申请日：2016-12-07

Applicant: Berkeley Lights, Inc.

Inventor： Kristin G. Beaumont ,  Peter J. Beemiller ,  Volker L. S. Kurz ,  Gregory G. Lavieu ,  Xiaohua Wang ,  Aathavan Karunakaran

IPC: B01L3/00 ,  G01N33/543 ,  G01N33/545 ,  G01N33/58

Abstract: In situ-generated microfluidic capture structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. Microfluidic capture structures may be advantageously used for assays performed within the microfluidic environment, providing flexibility in assaying micro-objects such as biological cells. Assay reagents and analytes may be incorporated within the microfluidic capture structures.

公开(公告)号：US20210011015A1

公开(公告)日：2021-01-14

申请号：US16908265

申请日：2020-06-22

Applicant: Berkeley Lights, Inc.

Inventor： Kristin G. Beaumont ,  Peter J. Beemiller ,  Volker L.S. Kurz ,  Gregory G. Lavieu ,  Xiaohua Wang ,  Aathavan Karunakaran

IPC: G01N33/543 ,  B01L3/00 ,  G01N33/545 ,  G01N33/58

Abstract: In situ-generated microfluidic capture structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. Microfluidic capture structures may be advantageously used for assays performed within the microfluidic environment, providing flexibility in assaying micro-objects such as biological cells. Assay reagents and analytes may be incorporated within the microfluidic capture structures.

公开(公告)号：US10723988B2

公开(公告)日：2020-07-28

申请号：US15135707

申请日：2016-04-22

Applicant: Berkeley Lights, Inc.

Inventor： Randall D. Lowe, Jr. ,  Kristin Beaumont ,  Aathavan Karunakaran ,  Natalie Marks ,  Jason M. McEwen ,  Mark P. White ,  J. Tanner Nevill ,  Gang F. Wang ,  Andrew W. McFarland ,  Daniele Malleo ,  Keith J. Breinlinger ,  Xiao Guan ,  Kevin T. Chapman

IPC: C12M1/00 ,  C12M3/00 ,  B01L3/00 ,  C12M3/06

Abstract: Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.

公开(公告)号：US20160312165A1

公开(公告)日：2016-10-27

申请号：US15135707

申请日：2016-04-22

Applicant: Berkeley Lights, Inc.

Inventor： Randall D. Lowe, JR. ,  Kristin Beaumont ,  Aathavan Karunakaran ,  Natalie Marks ,  Jason M. McEwen ,  Mark P. White ,  J. Tanner Nevill ,  Gang F. Wang ,  Andrew W. McFarland ,  Daniele Malleo ,  Keith J. Breinlinger ,  Xiao Guan ,  Kevin T. Chapman

IPC: C12M3/06 ,  C12N5/0783 ,  C12N5/16 ,  C12M1/00 ,  C12M3/00 ,  C12N5/09

CPC classification number: C12M23/16 ,  C12M29/10

Abstract: Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.

Abstract translation: 描述了用于在微流体装置中培养一个或多个生物细胞的系统，方法和试剂盒，包括提供配置成增强细胞生长，活力，可移植性或其任何组合的营养物和气体组分。 在一些实施方案中，培养单细胞可以在微流体装置中产生克隆群体。