公开(公告)号：US10407658B2

公开(公告)日：2019-09-10

申请号：US15282923

申请日：2016-09-30

申请人： BERKELEY LIGHTS, INC.

发明人： Russell A. Newstrom ,  Andrew W. McFarland ,  Darcy K. Kelly-Greene ,  J. Tanner Nevill ,  Gang F. Wang

IPC分类号： C12M1/00 ,  C12M1/02 ,  C12M1/32 ,  C12M1/34 ,  C12M1/36 ,  C12M3/00 ,  C12M1/12

摘要： Incubators including an enclosure with an internal chamber configured to support a cell culture plate comprising a plurality of wells are disclosed. The enclosure includes a plurality of openings configured to allow access to the wells. The incubators include a sealing element configured to seal the plurality of openings in the enclosure. The sealing element comprises a plurality of openings corresponding to at least a subset of the plurality of openings in the enclosure. Access to the internal chamber can be provided by aligning the plurality of openings in the sealing element with the plurality of openings in the enclosure. Methods for using the incubators are also provided.

公开(公告)号：US11971409B2

公开(公告)日：2024-04-30

申请号：US16892642

申请日：2020-06-04

申请人： Berkeley Lights, Inc.

发明人： Kevin T. Chapman ,  Mark P. White ,  Xiaohua Wang ,  Minha Park ,  Guido K. Stadler ,  Randall D. Lowe, Jr. ,  Xiao Guan Radstrom ,  Jason M. McEwen ,  Gang F. Wang ,  George L. Fox ,  Peggy A. Radel

IPC分类号： B01L3/00 ,  C07K16/00 ,  C07K16/28 ,  C07K16/30 ,  C12Q1/6886 ,  G01N33/50 ,  G01N33/543 ,  G01N33/569 ,  G01N33/574

CPC分类号： G01N33/574 ,  B01L3/502761 ,  C07K16/00 ,  C07K16/2887 ,  C07K16/30 ,  C12Q1/6886 ,  G01N33/5047 ,  G01N33/505 ,  G01N33/5052 ,  G01N33/54366 ,  G01N33/56972 ,  B01L2200/0647 ,  G01N2800/7028

摘要： A method of preparing an antibody therapeutic is provided comprising: (a) providing a dissociated cell sample from at least one solid tumor sample obtained from a patient; (b) loading the dissociated cell sample into a microfluidic device having a flow region and at least one isolation region fluidically connected to the flow region; (c) moving at least one B cell from the dissociated cell sample into at least one isolation region in the microfluidic device, thereby obtaining at least one isolated B cell; and (d) using the microfluidic device to identify at least one B cell that produces antibodies capable of binding to cancer cells. The cancer cells can be the patient's own cancer cells. Also provided are methods of treating patients, methods of labeling or detecting cancer, engineered T or NK cells comprising antibodies or fragments thereof, and engineered antibody constructs.

公开(公告)号：US20210069698A1

公开(公告)日：2021-03-11

申请号：US16928225

申请日：2020-07-14

申请人： Berkeley Lights, Inc.

发明人： Xiao Guan ,  Mark P. White ,  Jason M. McEwen ,  Gang F. Wang ,  Kevin T. Chapman ,  Xiaohua Wang ,  Christine E. Sun

IPC分类号： B01L3/00 ,  C12M1/02 ,  C12M1/34 ,  C12M1/36 ,  C12M1/00 ,  C12M3/06 ,  C12Q1/02 ,  G01N33/50

摘要： Functional assays using reporter cell assays are described which probe the activity of at least one cell of interest. The ability to probe at least one cell is provided by using the microfluidic methods, devices and kits described herein. Assays combining both reporter cell signaling as well as binding assay signaling for at least one cell is also described herein.

公开(公告)号：US10723988B2

公开(公告)日：2020-07-28

申请号：US15135707

申请日：2016-04-22

申请人： Berkeley Lights, Inc.

发明人： Randall D. Lowe, Jr. ,  Kristin Beaumont ,  Aathavan Karunakaran ,  Natalie Marks ,  Jason M. McEwen ,  Mark P. White ,  J. Tanner Nevill ,  Gang F. Wang ,  Andrew W. McFarland ,  Daniele Malleo ,  Keith J. Breinlinger ,  Xiao Guan ,  Kevin T. Chapman

IPC分类号： C12M1/00 ,  C12M3/00 ,  B01L3/00 ,  C12M3/06

摘要： Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.

公开(公告)号：US10712344B2

公开(公告)日：2020-07-14

申请号：US15406289

申请日：2017-01-13

申请人： Berkeley Lights, Inc.

发明人： Kevin T. Chapman ,  George L. Fox ,  Peggy A. Radel ,  Mark P. White ,  Xiaohua Wang ,  Minha Park ,  Guido K. Stadler ,  Randall D. Lowe, Jr. ,  Xiao Guan Radstrom ,  Jason M. McEwen ,  Gang F. Wang

IPC分类号： B01L3/00 ,  G01N33/574 ,  C07K16/00 ,  G01N33/569 ,  C07K16/28 ,  G01N33/50 ,  C07K16/30 ,  C12Q1/6886 ,  G01N33/543

摘要： A method of preparing an antibody therapeutic is provided comprising: (a) providing a dissociated cell sample from at least one solid tumor sample obtained from a patient; (b) loading the dissociated cell sample into a microfluidic device having a flow region and at least one isolation region fluidically connected to the flow region; (c) moving at least one B cell from the dissociated cell sample into at least one isolation region in the microfluidic device, thereby obtaining at least one isolated B cell; and (d) using the microfluidic device to identify at least one B cell that produces antibodies capable of binding to cancer cells. The cancer cells can be the patient's own cancer cells. Also provided are methods of treating patients, methods of labeling or detecting cancer, engineered T or NK cells comprising antibodies or fragments thereof, and engineered antibody constructs.

公开(公告)号：US20160312165A1

公开(公告)日：2016-10-27

申请号：US15135707

申请日：2016-04-22

申请人： Berkeley Lights, Inc.

发明人： Randall D. Lowe, JR. ,  Kristin Beaumont ,  Aathavan Karunakaran ,  Natalie Marks ,  Jason M. McEwen ,  Mark P. White ,  J. Tanner Nevill ,  Gang F. Wang ,  Andrew W. McFarland ,  Daniele Malleo ,  Keith J. Breinlinger ,  Xiao Guan ,  Kevin T. Chapman

IPC分类号： C12M3/06 ,  C12N5/0783 ,  C12N5/16 ,  C12M1/00 ,  C12M3/00 ,  C12N5/09

CPC分类号： C12M23/16 ,  C12M29/10

摘要： Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.

摘要翻译： 描述了用于在微流体装置中培养一个或多个生物细胞的系统，方法和试剂盒，包括提供配置成增强细胞生长，活力，可移植性或其任何组合的营养物和气体组分。 在一些实施方案中，培养单细胞可以在微流体装置中产生克隆群体。

公开(公告)号：US11203018B2

公开(公告)日：2021-12-21

申请号：US16160816

申请日：2018-10-15

申请人： Berkeley Lights, Inc.

发明人： Troy A. Lionberger ,  Matthew E. Fowler ,  Phillip J. M. Elms ,  Kevin D. Loutherback ,  Randall D. Lowe, Jr. ,  Jian Gong ,  J. Tanner Nevill ,  Gang F. Wang ,  Gregory G. Lavieu ,  John A. Tenney ,  Aathavan Karunakaran ,  Anupam Singhal ,  I-Jong Lin

IPC分类号： B01L3/00 ,  G01N21/64 ,  B03C5/02 ,  B03C5/00 ,  B01L9/00 ,  G06T7/00 ,  G01N21/05

摘要： Methods, systems and kits are described herein for detecting the results of an assay. In particular, the methods, systems and devices of the present disclosure rely on a difference between the diffusion rates of a reporter molecule and an analyte of interest in order to quantify an amount of analyte in a microfluidic device. The analyte may be a secreted product of a biological micro-object.

公开(公告)号：US10751715B1

公开(公告)日：2020-08-25

申请号：US15136481

申请日：2016-04-22

申请人： Berkeley Lights, Inc.

发明人： Xiao Guan ,  Mark P. White ,  Jason M. McEwen ,  Gang F. Wang ,  Kevin T. Chapman ,  Xiaohua Wang ,  Christine E. Sun

IPC分类号： B01L3/00 ,  C12M1/02 ,  C12M1/34 ,  C12M1/36 ,  C12M1/00 ,  C12M3/06 ,  C12Q1/02 ,  G01N33/50

摘要： Functional assays using reporter cell assays are described which probe the activity of at least one cell of interest. The ability to probe at least one cell is provided by using the microfluidic methods, devices and kits described herein. Assays combining both reporter cell signaling as well as binding assay signaling for at least one cell is also described herein.

公开(公告)号：US20200032193A1

公开(公告)日：2020-01-30

申请号：US16537389

申请日：2019-08-09

申请人： Berkeley Lights, Inc.

发明人： Russell A. NEWSTROM ,  Andrew W. McFarland ,  Darcy K. Kelly-Greene ,  J. Tanner Nevill ,  Gang F. Wang

IPC分类号： C12M1/00 ,  C12M1/12 ,  C12M1/32 ,  C12M3/00 ,  C12M1/02 ,  C12M1/34 ,  C12M1/36

摘要： Incubators including an enclosure with an internal chamber configured to support a cell culture plate comprising a plurality of wells are disclosed. The enclosure includes a plurality of openings configured to allow access to the wells. The incubators include a sealing element configured to seal the plurality of openings in the enclosure. The sealing element comprises a plurality of openings corresponding to at least a subset of the plurality of openings in the enclosure. Access to the internal chamber can be provided by aligning the plurality of openings in the sealing element with the plurality of openings in the enclosure. Methods for using the incubators are also provided.

公开(公告)号：US20200017817A1

公开(公告)日：2020-01-16

申请号：US16427952

申请日：2019-05-31

申请人： Berkeley Lights, Inc.

发明人： Darcy K. KELLY-GREENE ,  Russell A. Newstrom ,  Andrew W. McFarland ,  J. Tanner Nevill ,  Gang F. Wang

IPC分类号： C12M1/00 ,  C12M1/32 ,  C12M1/12 ,  C12M1/02

摘要： Incubators are disclosed which include an enclosure with an internal chamber configured to support a cell culture plate and provide an environment suitable for maintaining and/or culturing biological cells. The enclosure can include one or more openings configured to allow access to the cell culture plate. The incubators can further include a structure having a plurality of openings configured to be aligned with a corresponding plurality of wells in the cell culture plate, and a sealing element configured to moveably seal the plurality of openings in the structure. The sealing element can comprise a plurality of openings corresponding to at least a subset of the plurality of openings of the structure. Access to the internal chamber can be provided by aligning the plurality of openings in the sealing element with the plurality of openings in the structure. Methods for using the incubators are also provided.