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公开(公告)号:US11007520B2
公开(公告)日:2021-05-18
申请号:US16196649
申请日:2018-11-20
发明人: Randall D. Lowe, Jr. , Alexander J. Mastroianni , Mark P. White , Gregory G. Lavieu , Kristin G. Beaumont
IPC分类号: B01L3/00 , C12M3/06 , B81C1/00 , G01N33/543
摘要: In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces to provide improved or altered performance with biomaterials.
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2.
公开(公告)号:US20210011015A1
公开(公告)日:2021-01-14
申请号:US16908265
申请日:2020-06-22
发明人: Kristin G. Beaumont , Peter J. Beemiller , Volker L.S. Kurz , Gregory G. Lavieu , Xiaohua Wang , Aathavan Karunakaran
IPC分类号: G01N33/543 , B01L3/00 , G01N33/545 , G01N33/58
摘要: In situ-generated microfluidic capture structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. Microfluidic capture structures may be advantageously used for assays performed within the microfluidic environment, providing flexibility in assaying micro-objects such as biological cells. Assay reagents and analytes may be incorporated within the microfluidic capture structures.
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公开(公告)号:US20190275516A1
公开(公告)日:2019-09-12
申请号:US16196649
申请日:2018-11-20
发明人: Randall D. Lowe, JR. , Alexander J. Mastroianni , Mark P. White , Gregory G. Lavieu , Kristin G. Beaumont
摘要: In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces to provide improved or altered performance with biomaterials.
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公开(公告)号:US20190217297A1
公开(公告)日:2019-07-18
申请号:US16259538
申请日:2019-01-28
发明人: Gregory G. Lavieu , Annamaria Mocciaro , Xiao Guan Radstrom , Jason M. McEwen , Magali Soumillon , J. Tanner Nevill , Volker L.S. Kurz , Patricia A. Dyck , Ravi K. Ramenani
CPC分类号: B01L3/502761 , B01L3/502792 , B01L2200/0647 , B01L2200/0668 , B01L2300/0816 , B01L2300/0864 , B01L2400/0424 , B01L2400/0427 , C12M23/16 , C12N15/1003 , C12N15/1024 , C12N15/1086 , C12N2310/00 , C12N2510/00 , G01N15/10 , G01N2015/0288 , G01N2015/1081
摘要: Methods are described herein for isolating clonal populations of cells having a defined genetic modification. The methods are performed, at least in part, in a microfluidic device comprising one or more sequestration pens. The methods include the steps of: maintaining individual cells (or precursors thereof) that have undergone a genomic editing process in corresponding sequestration pens of a microfluidic device; expanding the individual cells into respective clonal populations of cells; and detecting, in one or more cells of each clonal population, the presence of a first nucleic acid sequence that is indicative of the presence of an on-target genome edit in the clonal population of cells. Also described are methods of performing genome editing within a microfluidic device, and compositions comprising one or more clonal populations of cells generated according to the methods disclosed herein.
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公开(公告)号:US20210291171A1
公开(公告)日:2021-09-23
申请号:US17162467
申请日:2021-01-29
发明人: Randall D. Lowe, JR. , Alexander J. Mastroianni , Mark P. White , Gregory G. Lavieu , Kristin G. Beaumont
IPC分类号: B01L3/00 , C12M3/06 , B81C1/00 , G01N33/543
摘要: In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces to provide improved or altered performance with biomaterials.
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公开(公告)号:US11103870B2
公开(公告)日:2021-08-31
申请号:US16259538
申请日:2019-01-28
发明人: Gregory G. Lavieu , Annamaria Mocciaro , Xiao Guan Radstrom , Jason M. McEwen , Magali Soumillon , J. Tanner Nevill , Volker L. S. Kurz , Patricia A. Dyck , Ravi K. Ramenani
摘要: Methods are described herein for isolating clonal populations of cells having a defined genetic modification. The methods are performed, at least in part, in a microfluidic device comprising one or more sequestration pens. The methods include the steps of: maintaining individual cells (or precursors thereof) that have undergone a genomic editing process in corresponding sequestration pens of a microfluidic device; expanding the individual cells into respective clonal populations of cells; and detecting, in one or more cells of each clonal population, the presence of a first nucleic acid sequence that is indicative of the presence of an on-target genome edit in the clonal population of cells. Also described are methods of performing genome editing within a microfluidic device, and compositions comprising one or more clonal populations of cells generated according to the methods disclosed herein.
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7.
公开(公告)号:US10705082B2
公开(公告)日:2020-07-07
申请号:US15372094
申请日:2016-12-07
发明人: Kristin G. Beaumont , Peter J. Beemiller , Volker L. S. Kurz , Gregory G. Lavieu , Xiaohua Wang , Aathavan Karunakaran
IPC分类号: B01L3/00 , G01N33/543 , G01N33/545 , G01N33/58
摘要: In situ-generated microfluidic capture structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. Microfluidic capture structures may be advantageously used for assays performed within the microfluidic environment, providing flexibility in assaying micro-objects such as biological cells. Assay reagents and analytes may be incorporated within the microfluidic capture structures.
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8.
公开(公告)号:US11454629B2
公开(公告)日:2022-09-27
申请号:US16908265
申请日:2020-06-22
发明人: Kristin G. Beaumont , Peter J. Beemiller , Volker L. S. Kurz , Gregory G. Lavieu , Xiaohua Wang , Aathavan Karunakaran
IPC分类号: B01L3/00 , G01N33/543 , G01N33/545 , G01N33/58 , G01N33/50
摘要: In situ-generated microfluidic capture structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. Microfluidic capture structures may be advantageously used for assays performed within the microfluidic environment, providing flexibility in assaying micro-objects such as biological cells. Assay reagents and analytes may be incorporated within the microfluidic capture structures.
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公开(公告)号:US10239058B2
公开(公告)日:2019-03-26
申请号:US15802174
申请日:2017-11-02
发明人: Gregory G. Lavieu , Annamaria Mocciaro , Xiao Guan Radstrom , Jason M. McEwen , Magali Soumillon , J. Tanner Nevill , Volker L. S. Kurz , Patricia A. Dyck , Ravi K. Ramenani
摘要: Methods are described herein for isolating clonal populations of cells having a defined genetic modification. The methods are performed, at least in part, in a microfluidic device comprising one or more sequestration pens. The methods include the steps of: maintaining individual cells (or precursors thereof) that have undergone a genomic editing process in corresponding sequestration pens of a microfluidic device; expanding the individual cells into respective clonal populations of cells; and detecting, in one or more cells of each clonal population, the presence of a first nucleic acid sequence that is indicative of the presence of an on-target genome edit in the clonal population of cells. Also described are methods of performing genome editing within a microfluidic device, and compositions comprising one or more clonal populations of cells generated according to the methods disclosed herein.
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10.
公开(公告)号:US20170184583A1
公开(公告)日:2017-06-29
申请号:US15372094
申请日:2016-12-07
发明人: Kristin G. Beaumont , Peter J. Beemiller , Volker L.S. Kurz , Gregory G. Lavieu , Xiaohua Wang , Aathavan Karunakaran
IPC分类号: G01N33/543 , G01N33/58 , G01N33/545
摘要: In situ-generated microfluidic capture structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. Microfluidic capture structures may be advantageously used for assays performed within the microfluidic environment, providing flexibility in assaying micro-objects such as biological cells. Assay reagents and analytes may be incorporated within the microfluidic capture structures.
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