公开(公告)号：US20180147576A1

公开(公告)日：2018-05-31

申请号：US15802174

申请日：2017-11-02

申请人： Berkeley Lights, Inc.

发明人： Gregory G. Lavieu ,  Annamaria Mocciaro ,  Xiao Guan Radstrom ,  Jason M. McEwen ,  Magali Soumillon ,  J. Tanner Nevill ,  Volker L.S. Kurz ,  Patricia A. Dyck ,  Ravi K. Ramenani

IPC分类号： B01L3/00 ,  C12M3/06 ,  C12N15/10 ,  G01N15/10

CPC分类号： B01L3/502761 ,  B01L3/502792 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0424 ,  B01L2400/0427 ,  C12M23/16 ,  C12N15/1003 ,  C12N15/1024 ,  C12N15/1086 ,  C12N2310/00 ,  C12N2510/00 ,  G01N15/10 ,  G01N2015/0288 ,  G01N2015/1081

摘要： Methods are described herein for isolating clonal populations of cells having a defined genetic modification. The methods are performed, at least in part, in a microfluidic device comprising one or more sequestration pens. The methods include the steps of: maintaining individual cells (or precursors thereof) that have undergone a genomic editing process in corresponding sequestration pens of a microfluidic device; expanding the individual cells into respective clonal populations of cells; and detecting, in one or more cells of each clonal population, the presence of a first nucleic acid sequence that is indicative of the presence of an on-target genome edit in the clonal population of cells. Also described are methods of performing genome editing within a microfluidic device, and compositions comprising one or more clonal populations of cells generated according to the methods disclosed herein.

公开(公告)号：US11103870B2

公开(公告)日：2021-08-31

申请号：US16259538

申请日：2019-01-28

申请人： Berkeley Lights, Inc.

发明人： Gregory G. Lavieu ,  Annamaria Mocciaro ,  Xiao Guan Radstrom ,  Jason M. McEwen ,  Magali Soumillon ,  J. Tanner Nevill ,  Volker L. S. Kurz ,  Patricia A. Dyck ,  Ravi K. Ramenani

IPC分类号： G01N15/10 ,  B01L3/00 ,  C12M3/06 ,  C12N15/10 ,  C12N15/00 ,  G01N15/02

摘要： Methods are described herein for isolating clonal populations of cells having a defined genetic modification. The methods are performed, at least in part, in a microfluidic device comprising one or more sequestration pens. The methods include the steps of: maintaining individual cells (or precursors thereof) that have undergone a genomic editing process in corresponding sequestration pens of a microfluidic device; expanding the individual cells into respective clonal populations of cells; and detecting, in one or more cells of each clonal population, the presence of a first nucleic acid sequence that is indicative of the presence of an on-target genome edit in the clonal population of cells. Also described are methods of performing genome editing within a microfluidic device, and compositions comprising one or more clonal populations of cells generated according to the methods disclosed herein.

公开(公告)号：US10239058B2

公开(公告)日：2019-03-26

申请号：US15802174

申请日：2017-11-02

申请人： Berkeley Lights, Inc.

发明人： Gregory G. Lavieu ,  Annamaria Mocciaro ,  Xiao Guan Radstrom ,  Jason M. McEwen ,  Magali Soumillon ,  J. Tanner Nevill ,  Volker L. S. Kurz ,  Patricia A. Dyck ,  Ravi K. Ramenani

IPC分类号： G01N15/00 ,  B01L3/00 ,  C12M3/06 ,  C12N15/10 ,  G01N15/10 ,  B03C7/02 ,  G01N15/02

摘要： Methods are described herein for isolating clonal populations of cells having a defined genetic modification. The methods are performed, at least in part, in a microfluidic device comprising one or more sequestration pens. The methods include the steps of: maintaining individual cells (or precursors thereof) that have undergone a genomic editing process in corresponding sequestration pens of a microfluidic device; expanding the individual cells into respective clonal populations of cells; and detecting, in one or more cells of each clonal population, the presence of a first nucleic acid sequence that is indicative of the presence of an on-target genome edit in the clonal population of cells. Also described are methods of performing genome editing within a microfluidic device, and compositions comprising one or more clonal populations of cells generated according to the methods disclosed herein.

公开(公告)号：US20190217297A1

公开(公告)日：2019-07-18

申请号：US16259538

申请日：2019-01-28

申请人： Berkeley Lights, Inc.

发明人： Gregory G. Lavieu ,  Annamaria Mocciaro ,  Xiao Guan Radstrom ,  Jason M. McEwen ,  Magali Soumillon ,  J. Tanner Nevill ,  Volker L.S. Kurz ,  Patricia A. Dyck ,  Ravi K. Ramenani

IPC分类号： B01L3/00 ,  G01N15/10 ,  C12N15/10 ,  C12M3/06

CPC分类号： B01L3/502761 ,  B01L3/502792 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0424 ,  B01L2400/0427 ,  C12M23/16 ,  C12N15/1003 ,  C12N15/1024 ,  C12N15/1086 ,  C12N2310/00 ,  C12N2510/00 ,  G01N15/10 ,  G01N2015/0288 ,  G01N2015/1081

摘要： Methods are described herein for isolating clonal populations of cells having a defined genetic modification. The methods are performed, at least in part, in a microfluidic device comprising one or more sequestration pens. The methods include the steps of: maintaining individual cells (or precursors thereof) that have undergone a genomic editing process in corresponding sequestration pens of a microfluidic device; expanding the individual cells into respective clonal populations of cells; and detecting, in one or more cells of each clonal population, the presence of a first nucleic acid sequence that is indicative of the presence of an on-target genome edit in the clonal population of cells. Also described are methods of performing genome editing within a microfluidic device, and compositions comprising one or more clonal populations of cells generated according to the methods disclosed herein.

公开(公告)号：US11273177B2

公开(公告)日：2022-03-15

申请号：US15488139

申请日：2017-04-14

申请人： Berkeley Lights, Inc.

发明人： Kevin T Chapman ,  Xiaohua Wang ,  Xiao Guan Radstrom ,  Yelena Bronevetsky ,  Guido K Stadler ,  Gregory G Lavieu ,  Annamaria Mocciaro

IPC分类号： A61K35/17 ,  C07K14/55 ,  C12N9/24 ,  C07K16/28 ,  B01L3/00 ,  C07K14/195 ,  C12N5/0783 ,  A61K31/65 ,  C12N5/00 ,  C12N5/0781 ,  C07K14/47 ,  C07K14/52 ,  A61K39/00

摘要： The present disclosure provides methods of preparing tumor infiltrating cells engineered to express a pro-inflammatory polypeptide. The pro-inflammatory polypeptide is expressed from the tumor infiltrating cell to counter a generally immunosuppressive state in and around tumors resulting from an imbalance between the number and activation state of immune effector cells versus those of suppressor cells. Delivering the proinflammatory polypeptide via expression from the TICs, as distinct from systemic administration, reduces side effects from increased inflammation at sides remote from a tumor to be treated.

公开(公告)号：US10712344B2

公开(公告)日：2020-07-14

申请号：US15406289

申请日：2017-01-13

申请人： Berkeley Lights, Inc.

发明人： Kevin T. Chapman ,  George L. Fox ,  Peggy A. Radel ,  Mark P. White ,  Xiaohua Wang ,  Minha Park ,  Guido K. Stadler ,  Randall D. Lowe, Jr. ,  Xiao Guan Radstrom ,  Jason M. McEwen ,  Gang F. Wang

IPC分类号： B01L3/00 ,  G01N33/574 ,  C07K16/00 ,  G01N33/569 ,  C07K16/28 ,  G01N33/50 ,  C07K16/30 ,  C12Q1/6886 ,  G01N33/543

摘要： A method of preparing an antibody therapeutic is provided comprising: (a) providing a dissociated cell sample from at least one solid tumor sample obtained from a patient; (b) loading the dissociated cell sample into a microfluidic device having a flow region and at least one isolation region fluidically connected to the flow region; (c) moving at least one B cell from the dissociated cell sample into at least one isolation region in the microfluidic device, thereby obtaining at least one isolated B cell; and (d) using the microfluidic device to identify at least one B cell that produces antibodies capable of binding to cancer cells. The cancer cells can be the patient's own cancer cells. Also provided are methods of treating patients, methods of labeling or detecting cancer, engineered T or NK cells comprising antibodies or fragments thereof, and engineered antibody constructs.

公开(公告)号：US20170224734A1

公开(公告)日：2017-08-10

申请号：US15488139

申请日：2017-04-14

申请人： Berkeley Lights, Inc.

发明人： Kevin T Chapman ,  Xiaohua Wang ,  Xiao Guan Radstrom ,  Yelena Bronevetsky ,  Guido K Stadler ,  Gregory G Levieu ,  Annamaria Mocciaro

IPC分类号： A61K35/17 ,  C07K14/55 ,  C12N9/24 ,  C07K16/28 ,  B01L3/00 ,  C07K14/195 ,  C12N5/0783 ,  A61K31/65

CPC分类号： A61K35/17 ,  A61K31/65 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2300/0861 ,  B01L2300/0896 ,  B01L2400/0424 ,  C07K14/195 ,  C07K14/4748 ,  C07K14/52 ,  C07K14/55 ,  C07K16/2818 ,  C07K2317/14 ,  C07K2319/00 ,  C12N5/00 ,  C12N5/0635 ,  C12N5/0636 ,  C12N5/0637 ,  C12N5/0638 ,  C12N9/2402 ,  C12N2501/04 ,  C12N2510/00 ,  C12Y302/01166

摘要： The present disclosure provides methods of preparing tumor infiltrating cells engineered to express a pro-inflammatory polypeptide. The pro-inflammatory polypeptide is expressed from the tumor infiltrating cell to counter a generally immunosuppressive state in and around tumors resulting from an imbalance between the number and activation state of immune effector cells versus those of suppressor cells. Delivering the proinflammatory polypeptide via expression from the TICs, as distinct from systemic administration, reduces side effects from increased inflammation at sides remote from a tumor to be treated.