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    METHOD FOR THE PREPARATION AND EXTRACTION OF SQUALENE FROM MICROALGAE
    2.
    发明申请
    METHOD FOR THE PREPARATION AND EXTRACTION OF SQUALENE FROM MICROALGAE 审中-公开
    从微藻中制备和提取平均分子量的方法

    公开(公告)号：US20140088201A1

    公开(公告)日：2014-03-27

    申请号：US14118641

    申请日：2012-05-18

    申请人： Bernard Pora ,  Yun Qian ,  Bernard Caulier ,  Serge Comini ,  Philippe Looten ,  Laurent Segueilha

    发明人： Bernard Pora ,  Yun Qian ,  Bernard Caulier ,  Serge Comini ,  Philippe Looten ,  Laurent Segueilha

    IPC分类号： C12P5/02

    CPC分类号： C12P5/026 ,  C12N1/12 ,  C12P5/007 ,  C12P5/02

    摘要： The invention relates to a method for the production of squalene from microalgae belonging to the family of Thraustochytriales sp., preferably at concentrations of between 2 and 12 g per 100 g of dry biomass. The method is characterised in that it comprises steps consisting in: culturing microalgae belonging to the family of Thraustochytriales sp. at a temperature of between 25 and 35° C., preferably between 28 and 32° C., and more preferably of the order of 30° C.; and adding between 1 and 1000 μg of vitamin B12 per litre of culture medium to said culture medium.

    摘要翻译： 本发明涉及从属于Thraustochytriales sp。家族的微藻生产角鲨烯的方法，优选浓度为每100g干生物量为2至12g。 该方法的特征在于它包括以下步骤：培养属于Thraustochytriales sp。 在25至35℃之间的温度下，优选28至32℃，更优选约30℃。 并将1升至1000微克维生素B12 /升培养基加入到所述培养基中。

    Method of producing erythritol by repeated fed-batch fermentation
    5.
    发明授权
    Method of producing erythritol by repeated fed-batch fermentation 失效
    通过重复补料分批发酵生产赤藓糖醇的方法

    公开(公告)号：US06365383B1

    公开(公告)日：2002-04-02

    申请号：US09337909

    申请日：1999-06-22

    申请人： Laurent Segueilha

    发明人： Laurent Segueilha

    IPC分类号： C12P718

    CPC分类号： C12P7/18 ,  Y02P20/582

    摘要： The invention relates to a method of producing erythritol by fed-batch and repeated fermentation of sugars by microorganisms which produce erythritol, characterised by the fact that the fermentation is started by introducing said microorganisms into a fermentation medium containing a concentration of sugars lower than 200 g/l, and the fermentation is continued by carrying out at least one fermentation cycle which comprises: at least one phase of adding substrates so as to maintain the concentration of total sugars at a value of between 0 and 200 g/l, at least one phase of separation of the fermentation medium into a concentrated fraction of microorganisms and another fraction enriched with erythritol, when there is total consumption of sugars, at least one phase of recycling recovered and concentrated microorganisms in the fermentation medium.

    摘要翻译： 本发明涉及通过补料分批并通过产生赤藓糖醇的微生物重复发酵糖来生产赤藓糖醇的方法，其特征在于通过将所述微生物引入含有低于200g糖的浓度的发酵培养基来开始发酵 通过进行至少一个发酵循环来继续发酵，所述发酵循环包括：添加底物的至少一个相，以便将总糖的浓度保持在0至200g / l的值，至少一个 当总消耗糖，至少一个回收阶段回收的发酵培养基中的浓缩微生物和发酵培养基中的浓缩微生物时，将发酵培养基分离成微生物浓缩部分和富含赤藓糖醇的另一部分。

    Process for the production of 1,3-propanediol by fermentation
    6.
    发明授权
    Process for the production of 1,3-propanediol by fermentation 有权
    通过发酵生产1,3-丙二醇的方法

    公开(公告)号：US06406895B1

    公开(公告)日：2002-06-18

    申请号：US09696622

    申请日：2000-10-25

    申请人： Sophie Defretin ,  Brigitte Delelis ,  Laurent Segueilha

    发明人： Sophie Defretin ,  Brigitte Delelis ,  Laurent Segueilha

    IPC分类号： C12P718

    CPC分类号： C12P7/18

    摘要： The present invention relates to a process for the production of 1,3-propanediol by the fermentation of a 1,3-propanediol-producing microorganism in a fermentation medium containing glucose, characterized in that the fermentation is carried out without mechanical agitation, with the maintenance of an air retention greater than or equal to 50%, expressed as the volume of gas relative to the total volume of the liquid phase of the fermentation medium, and with the maintenance of a high cell density and a microorganism viability value, determined by a test A, greater than or equal to 95%, preferably of between 95 and 99%, by controlling frothing in the fermentation medium.

    摘要翻译： 本发明涉及通过在含有葡萄糖的发酵培养基中发酵1,3-丙二醇生产微生物来生产1,3-丙二醇的方法，其特征在于发酵在没有机械搅拌的情况下进行， 维持空气保持率大于或等于50％，表示为相对于发酵培养基液相总体积的气体体积，并维持高细胞密度和微生物活力值，由 通过控制发酵培养基中的发泡，测试A大于或等于95％，优选在95至99％之间。

    Method of producing arabitol by continuous fermentation
    7.
    发明授权
    Method of producing arabitol by continuous fermentation 有权
    通过连续发酵生产阿拉伯糖醇的方法

    公开(公告)号：US06268190B1

    公开(公告)日：2001-07-31

    申请号：US09567386

    申请日：2000-05-09

    申请人： Pierrick Duflot ,  Pierre Lanos ,  Fabrice Machu ,  Laurent Segueilha

    发明人： Pierrick Duflot ,  Pierre Lanos ,  Fabrice Machu ,  Laurent Segueilha

    IPC分类号： C12P718

    CPC分类号： C12P7/18 ,  Y10S435/813 ,  Y10S435/911 ,  Y10S435/921 ,  Y10S435/93 ,  Y10S435/94

    摘要： A method of producing arabitol by continuous fermentation of at least one sugar by micro-organisms producing arabitol, characterised in that arabitol is produced in a first fermentation area including at least one fermenter in such a manner that some of the sugar introduced into the fermentation medium is consumed by said micro-organisms, some of the fermentation medium obtained in this way is transferred into a second fermentation area including at least one fermenter, the volume being maintained constant in the first fermentation area by adding sugar, production of arabitol continues in said second fermentation area in such a manner as to consume the residual sugar of the fermentation medium, the fermentation medium thus obtained from said second fermentation area is separated a continuously into a fraction concentrated in micro-organisms and another, soluble fraction enriched in arabitol, and the arabitol thus obtained is collected.

    摘要翻译： 一种通过生产阿拉伯糖醇的微生物连续发酵至少一种糖的阿拉伯糖醇的方法，其特征在于在包括至少一个发酵罐的第一发酵区域中产生阿糖醇，使得一些糖引入发酵培养基 被所述微生物消耗，将以这种方式获得的一些发酵培养基转移到包括至少一个发酵罐的第二发酵区域中，该体积通过加入糖在第一发酵区域保持恒定，所述 第二发酵区域以消耗发酵培养基的残留糖的方式，将从所述第二发酵区域获得的发酵培养基连续分离成浓缩在微生物中的级分，另一种富含阿拉伯糖醇的可溶级分，以及 收集如此得到的阿糖醇。