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公开(公告)号：US08486686B2
公开(公告)日：2013-07-16
申请号：US12747979
申请日：2007-12-28
申请人： Laurent Segueilha , Ka-Yiu San , George Bennett , Irene Martinez
发明人： Laurent Segueilha , Ka-Yiu San , George Bennett , Irene Martinez
IPC分类号： C12N1/21
CPC分类号： C12P7/46 , C12N1/20 , C12P7/04 , C12P7/065 , C12P7/16 , C12P7/20 , C12P7/56 , Y02E50/10 , Y02E50/17
摘要： A new culture method for producing high levels of a metabolite, such as succinic acid uses oxygen rich culture without pH adjustment to increase the biomass, acclimation in under oxygen lean conditions having
摘要翻译： 用于生产高含量代谢物（如琥珀酸）的新培养方法使用富含氧气的培养物，无需pH调节，以增加生物量，适应氧气分压低于氧气的氧气条件下，以及产生高水平 琥珀酸在氧气剥夺条件下。 该方法可以在单个反应器中进行，并且可以有效地进行放大。
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公开(公告)号：US08906667B2
公开(公告)日：2014-12-09
申请号：US12439497
申请日：2007-08-29
申请人： Ka-Yiu San , George Bennett , Henry Lin , Irene Martinez , Jiangfeng Zhu
发明人： Ka-Yiu San , George Bennett , Henry Lin , Irene Martinez , Jiangfeng Zhu
IPC分类号： C12N1/20 , C12N15/00 , C12N9/04 , C12N9/02 , C12N9/14 , C07H21/04 , C07K14/315 , C12N15/70 , A61K38/48 , C07K14/30 , C07K14/33 , C07K14/395 , C12P17/06 , C12P23/00
CPC分类号： C07K14/315 , A61K38/484 , C07K14/30 , C07K14/33 , C07K14/395 , C12N9/0006 , C12N15/70 , C12P17/06 , C12P23/00
摘要： A method of increasing cellular NADPH levels by expressing one or more genes that encode an enzyme that causes the production of NADPH. The system is combined with other enzymes that require NADPH, thus improving the overall yield of the desired product.
摘要翻译： 通过表达编码引起NADPH产生的酶的一种或多种基因来增加细胞NADPH水平的方法。 该系统与需要NADPH的其它酶组合，从而提高所需产物的总产率。
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公开(公告)号：US20100009418A1
公开(公告)日：2010-01-14
申请号：US12439497
申请日：2007-08-29
申请人： Ka-Yiu San , George Bennett , Henry Lin , Irene Martinez , Jiangfeng Zhu
发明人： Ka-Yiu San , George Bennett , Henry Lin , Irene Martinez , Jiangfeng Zhu
CPC分类号： C07K14/315 , A61K38/484 , C07K14/30 , C07K14/33 , C07K14/395 , C12N9/0006 , C12N15/70 , C12P17/06 , C12P23/00
摘要： A method of increasing cellular NADPH levels by expressing one or more genes that encode an enzyme that causes the production of NADPH. The system is combined with other enzymes that require NADPH, thus improving the overall yield of the desired product.
摘要翻译： 通过表达编码引起NADPH产生的酶的一种或多种基因来增加细胞NADPH水平的方法。 该系统与需要NADPH的其它酶组合，从而提高所需产物的总产率。
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公开(公告)号：US09562224B2
公开(公告)日：2017-02-07
申请号：US12439504
申请日：2007-09-07
申请人： Ka-Yiu San , George Bennett
发明人： Ka-Yiu San , George Bennett
IPC分类号： C12N1/20 , C12N15/00 , C12N9/00 , C12N9/02 , C12N9/04 , C12P7/00 , C12N9/88 , C12N9/10 , C12P1/04
CPC分类号： C12N9/88 , C12N1/20 , C12N9/1085 , C12P1/04 , Y02P20/52
摘要： Production of products by engineered bacteria is increased by regulating cellular respiration. Cellular respiration is controlled by reducing electron transfer enzyme activity. Some examples of electron transfer enzymes include NADH dehydrogenases, Succinate dehydrogenases, ubiquinone synthesis, cytochrome O, and cytochrome D. In one example, deletion of UbiCA prevents respiration. Respiration can the be controlled by addition of ubiquinone or expression of ubiCA.
摘要翻译： 通过调节细胞呼吸来增加由工程细菌产生的产物。 通过减少电子转移酶活性来控制细胞呼吸。 电子转移酶的一些实例包括NADH脱氢酶，琥珀酸脱氢酶，泛醌合成，细胞色素O和细胞色素D.在一个实例中，UbiCA的缺失可防止呼吸。 可以通过添加泛醌或ubiCA的表达来控制呼吸。
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公开(公告)号：US20100009403A1
公开(公告)日：2010-01-14
申请号：US12439504
申请日：2007-09-07
申请人： Ka-Yiu San , George Bennett
发明人： Ka-Yiu San , George Bennett
CPC分类号： C12N9/88 , C12N1/20 , C12N9/1085 , C12P1/04 , Y02P20/52
摘要： Production of products by engineered bacteria is increased by regulating cellular respiration. Cellular respiration is controlled by reducing electron transfer enzyme activity. Some examples of electron transfer enzymes include NADH dehydrogenases, Succinate dehydrogenases, ubiquinone synthesis, cytochrome O, and cytochrome D. In one example, deletion of UbiCA prevents respiration. Respiration can the be controlled by addition of ubiquinone or expression of ubiCA.
摘要翻译： 通过调节细胞呼吸来增加由工程细菌产生的产物。 通过减少电子转移酶活性来控制细胞呼吸。 电子转移酶的一些实例包括NADH脱氢酶，琥珀酸脱氢酶，泛醌合成，细胞色素O和细胞色素D.在一个实例中，UbiCA的缺失可防止呼吸。 可以通过添加泛醌或ubiCA的表达来控制呼吸。
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公开(公告)号：US20060040368A1
公开(公告)日：2006-02-23
申请号：US11200385
申请日：2005-08-09
申请人： Ka-Yiu San , George Bennett , Henry Lin
发明人： Ka-Yiu San , George Bennett , Henry Lin
摘要： Methods of increasing yields of succinate using aerobic culture methods and a multi-mutant E. coli strain are provided. Also provided is a mutant strain of E. coli that produces high amounts of succinic acid.
摘要翻译： 提供了使用需氧培养方法和多突变大肠杆菌菌株提高琥珀酸产量的方法。 还提供了产生大量琥珀酸的大肠杆菌的突变菌株。
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公开(公告)号：US20050196866A1
公开(公告)日：2005-09-08
申请号：US10987265
申请日：2004-11-12
申请人： Ka-Yiu San , George Bennett , Ailen Sanchez
发明人： Ka-Yiu San , George Bennett , Ailen Sanchez
CPC分类号： C12P19/36
摘要： A method of increasing cellular NADPH levels by expressing one or more genes that encode a enzyme that causes the production of NADPH. The system can be combined with other enzymes that require NADPH, thus improving the overall production of the desired protein.
摘要翻译： 通过表达编码引起NADPH产生的酶的一种或多种基因来增加细胞NADPH水平的方法。 该系统可以与需要NADPH的其它酶组合，从而提高所需蛋白质的总体产量。
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公开(公告)号：US20080009034A1
公开(公告)日：2008-01-10
申请号：US11773408
申请日：2007-07-04
CPC分类号： C12N9/0036 , C12P1/00 , C12P7/625
摘要： The present invention describes a novel recombinant NADH recycling system that is used as a process for producing reduced compounds. In a specific embodiment, the reduced compounds include ethanol, succinate, lactate, a vitamin, a pharmaceutical and a biodegraded organic molecule. The NADH recycling system effects metabolic flux of reductive pathways in aerobic and anaerobic environments.
摘要翻译： 本发明描述了一种用作生产还原化合物的方法的新型重组NADH再循环系统。 在具体实施方案中，还原的化合物包括乙醇，琥珀酸盐，乳酸盐，维生素，药物和生物降解的有机分子。 NADH回收系统在有氧和厌氧环境中影响还原途径的代谢通量。
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公开(公告)号：US08795991B2
公开(公告)日：2014-08-05
申请号：US13696268
申请日：2011-05-03
申请人： Ka-Yiu San , George Bennett , Grant Balzer , Jiangfeng Zhu , Chandresh Thakker , Ailen Sanchez
发明人： Ka-Yiu San , George Bennett , Grant Balzer , Jiangfeng Zhu , Chandresh Thakker , Ailen Sanchez
IPC分类号： C12N15/70
CPC分类号： C12P7/46 , C12N9/0008 , C12N9/93 , C12N15/52 , C12N15/70 , C12Y102/01002 , C12Y604/01001
摘要： Improved bacteria for making succinate and other 4 carbon dicarboxylates from the Krebs cycle have modifications to reduce acetate, lactate, EtOH and formate, as well as turn on the glyoxylate shunt, produce more NADH and overexpress In one embodiment, the bacteria are ΔadhEΔldhAΔiclRΔack-pta plus PYC+ and NAD+-dependant FDH+.
摘要翻译： 用于制备琥珀酸盐和其他来自克雷伯斯循环的4碳二羧酸盐的细菌具有改变以减少乙酸盐，乳酸盐，乙醇和甲酸盐，以及开启乙醛酸盐分流，产生更多的NADH和过表达。在一个实施方案中，细菌是＆Dgr; adhE＆Dgr ; ldhA＆Dgr; iclR＆Dgr; ack-pta加PYC +和NAD +依赖性FDH +。
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公开(公告)号：US20060141594A1
公开(公告)日：2006-06-29
申请号：US11315453
申请日：2005-12-22
申请人： Ka-Yiu San , Ailen Sanchez , George Bennett , Cheryl Dittrich
发明人： Ka-Yiu San , Ailen Sanchez , George Bennett , Cheryl Dittrich
CPC分类号： C12N9/0006 , C12N9/1029 , C12P7/46 , C12P7/62
摘要： In vivo method of producing esters from acetyle coA, such as isoamyl acetate and succinate, has been developed by producing null mutants in pathways that use acetyl coA and by overexpressing products that use NADH and in order to maintain the proper redox balance between NADH and NAD+. The method is exemplified with null mutations in ldhA, adhE, ackA-pta and overexpression of pyruvate carboxylase and alcohol acetyltransferase. This strain produces higher levels of both isoamyl acetate and succinate.
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