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    DNA which regulates gene expression in coryneform bacteria
    3.
    发明授权
    DNA which regulates gene expression in coryneform bacteria 失效
    调节棒状细菌基因表达的DNA

    公开(公告)号：US5965391A

    公开(公告)日：1999-10-12

    申请号：US836943

    申请日：1997-05-08

    申请人： Dieter Reinscheid ,  Bernhard Eikmanns ,  Hermann Sahm

    发明人： Dieter Reinscheid ,  Bernhard Eikmanns ,  Hermann Sahm

    IPC分类号： C12N15/09 ,  C12N1/21 ,  C12N9/88 ,  C12N15/31 ,  C12N15/77 ,  C12P21/00 ,  C12R1/15 ,  C07H21/04

    CPC分类号： C12N15/77 ,  C12N9/88

    摘要： The invention concerns a DNA fragment located in front of the malate synthase gene of a coryne-form bacterium and isolated from the latter. Any structural gene which codes for a protein can be inserted after this DNA fragment. After transformation of such a construct into a coryne-form bacterium, expression of the structural gene inserted after the DNA fragment is regulated. The invention also concerns a process for synthesizing any protein by culturing a transformed coryne-form bacterium. A bacterium of this type contains in replicable form a DNA fragment isolated from the malate synthase gene of a coryne-form bacterium, and after which the structural gene which codes for the protein to be synthesized is inserted. Since expression of the structural gene which codes for the protein to be synthesized is regulated by the DNA located in front of it, the structural gene is expressed and the desired protein synthesized as soon as a suitable inducing agent is added to the medium.

    摘要翻译： PCT No.PCT / DE95 / 01555 Sec。 371日期：1997年5月8日 102（e）日期1997年5月8日PCT提交1995年11月7日PCT公布。 公开号WO96 / 15246 日期1996年5月23日本发明涉及一种位于棒状细菌的苹果酸合酶基因前面并从后者分离的DNA片段。 可以在该DNA片段之后插入编码蛋白质的任何结构基因。 在将这种构建体转化成棒状细菌后，调节在DNA片段之后插入的结构基因的表达。 本发明还涉及通过培养转化的棒状细菌合成任何蛋白质的方法。 这种细菌以可复制形式包含从棒状细菌的苹果酸合酶基因分离的DNA片段，之后插入编码待合成蛋白质的结构基因。 由于编码要合成的蛋白质的结构基因的表达由位于其前面的DNA调节，所以结构基因被表达，并且一旦合适的诱导剂加入培养基中就合成所需的蛋白质。