公开(公告)号：US10279350B2

公开(公告)日：2019-05-07

申请号：US15365894

申请日：2016-11-30

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Benjamin J. Hindson ,  Kevin D. Ness ,  Billy W. Colston, Jr. ,  Fred P. Milanovich ,  Donald A. Masquelier

IPC: B01L3/00 ,  B01F13/00 ,  B01F15/00 ,  B01L3/02 ,  B01L7/00 ,  B29C45/00 ,  C12Q1/686 ,  G01N21/3563 ,  G01N21/49 ,  G01N21/64 ,  B01F3/08 ,  B29L31/00

CPC classification number: B01L3/502784 ,  B01F3/0807 ,  B01F13/0062 ,  B01F15/00922 ,  B01F2003/0834 ,  B01F2003/0842 ,  B01F2215/0037 ,  B01L3/0241 ,  B01L3/502715 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/0673 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/041 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0858 ,  B01L2300/0867 ,  B01L2300/1822 ,  B01L2400/0478 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0622 ,  B29C45/0053 ,  B29C45/006 ,  B29C2045/0079 ,  B29L2031/752 ,  C12Q1/686 ,  G01N21/3563 ,  G01N21/49 ,  G01N21/6428 ,  G01N21/6486 ,  G01N2021/6439 ,  Y02A90/26

Abstract: Methods of generating droplets. In an exemplary method, a device including a sample well, a carrier well, a droplet well, and a plurality of microfluidic channels is selected. The microfluidic channels include a first channel, a second channel, and a third channel. A discrete volume of sample-containing fluid is placed into the sample well, and a discrete volume of carrier fluid is placed into the carrier well. A pressure differential is created after placing the discrete volumes, to cause fluid flow. Sample-containing fluid flows from the sample well to a droplet-generation region of the device via the first channel. Carrier fluid flows from the carrier well to the droplet-generation region via the second channel. Sample-containing droplets and carrier fluid flow from the droplet-generation region to the droplet well via the third channel.

公开(公告)号：US09901927B2

公开(公告)日：2018-02-27

申请号：US15365894

申请日：2016-11-30

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Benjamin J. Hindson ,  Kevin D. Ness ,  Billy W. Colston, Jr. ,  Fred P. Milanovich ,  Donald A. Masquelier

IPC: B01L3/00 ,  B01F3/08 ,  B01F13/00 ,  B01L3/02 ,  B01L7/00 ,  G01N21/3563 ,  C12Q1/68 ,  G01N21/64 ,  B01F15/00 ,  B29C45/00 ,  G01N21/49 ,  B29L31/00

Abstract: Methods of generating droplets. In an exemplary method, a device including a sample well, a carrier well, a droplet well, and a plurality of microfluidic channels is selected. The microfluidic channels include a first channel, a second channel, and a third channel. A discrete volume of sample-containing fluid is placed into the sample well, and a discrete volume of carrier fluid is placed into the carrier well. A pressure differential is created after placing the discrete volumes, to cause fluid flow. Sample-containing fluid flows from the sample well to a droplet-generation region of the device via the first channel. Carrier fluid flows from the carrier well to the droplet-generation region via the second channel. Sample-containing droplets and carrier fluid flow from the droplet-generation region to the droplet well via the third channel.

公开(公告)号：US20170144161A1

公开(公告)日：2017-05-25

申请号：US15365894

申请日：2016-11-30

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Benjamin J. Hindson ,  Kevin D. Ness ,  Billy W. Colston, JR. ,  Fred P. Milanovich ,  Donald A. Masquelier ,  Anthony J. Makarewicz, JR.

IPC: B01L3/00 ,  B01F13/00 ,  G01N21/64 ,  B29C45/00 ,  C12Q1/68 ,  B01L7/00 ,  B01F3/08 ,  B01F15/00

CPC classification number: B01L3/502784 ,  B01F3/0807 ,  B01F13/0062 ,  B01F15/00922 ,  B01F2003/0834 ,  B01F2003/0842 ,  B01F2215/0037 ,  B01L3/0241 ,  B01L3/502715 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/0673 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/041 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0858 ,  B01L2300/0867 ,  B01L2300/1822 ,  B01L2400/0478 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0622 ,  B29C45/0053 ,  B29C45/006 ,  B29C2045/0079 ,  B29L2031/752 ,  C12Q1/686 ,  G01N21/3563 ,  G01N21/49 ,  G01N21/6428 ,  G01N21/6486 ,  G01N2021/6439 ,  G06F19/00 ,  Y02A90/26

Abstract: Methods of generating droplets. In an exemplary method, a device including a sample well, a carrier well, a droplet well, and a plurality of microfluidic channels is selected. The microfluidic channels include a first channel, a second channel, and a third channel. A discrete volume of sample-containing fluid is placed into the sample well, and a discrete volume of carrier fluid is placed into the carrier well. A pressure differential is created after placing the discrete volumes, to cause fluid flow. Sample-containing fluid flows from the sample well to a droplet-generation region of the device via the first channel. Carrier fluid flows from the carrier well to the droplet-generation region via the second channel. Sample-containing droplets and carrier fluid flow from the droplet-generation region to the droplet well via the third channel.