公开(公告)号：US20120107904A1

公开(公告)日：2012-05-03

申请号：US12431315

申请日：2009-04-28

申请人： Brian G. Fox ,  Yong Chang ,  Gary A. Wesenberg ,  Craig A. Bingman

发明人： Brian G. Fox ,  Yong Chang ,  Gary A. Wesenberg ,  Craig A. Bingman

IPC分类号： C12N9/02 ,  G06G7/58 ,  C12N1/21 ,  C12N15/53 ,  C12N15/63

CPC分类号： C12N9/0071 ,  C07K2319/00 ,  C07K2319/21 ,  C07K2319/31 ,  C07K2319/41 ,  C07K2319/60 ,  C12Y114/19001

摘要： Compositions and methods are provided that are useful for predicting and controlling the stability of expressed polypeptides. The compositions and methods may be used to predict and as desired, increase or decrease the stability of proteins recombinantly expressed in mycobacteria, for example DesA3 expressed in Mycobacterium smegmatis. At the C terminus and the penultimate position, substitution to residues with charged side chains, large non-polar side chains, or no side chains can be used to reduce or inhibit the protein degradation. At the antepenultimate position from the C terminus, residues with no side chain or acidic side chains can increase the stability, i.e. reduce or inhibit the protein degradation. The combinational substitution of only the last three residues of polypeptides can make the polypeptides more stable during heterologous expression in mycobacterial hosts.

摘要翻译： 提供了可用于预测和控制表达多肽的稳定性的组合物和方法。 组合物和方法可以用于预测和按需要增加或降低在分枝杆菌中重组表达的蛋白质的稳定性，例如在耻垢分枝杆菌中表达的DesA3。 在C末端和倒数第二的位置，可以使用带有带侧链，大非极性侧链或无侧链的残基取代以降低或抑制蛋白质降解。 在C末端的最初位置，没有侧链或酸性侧链的残基可以增加稳定性，即降低或抑制蛋白质降解。 仅在多肽的最后3个残基的组合取代可以使多肽在分枝杆菌宿主的异源表达期间更稳定。

公开(公告)号：US20080199869A1

公开(公告)日：2008-08-21

申请号：US11899080

申请日：2007-09-04

申请人： Brian G. Fox ,  Yong Chang

发明人： Brian G. Fox ,  Yong Chang

IPC分类号： C07K16/00 ,  C12Q1/68

CPC分类号： C12N9/0083 ,  C07K2319/21 ,  C07K2319/50 ,  C12N9/004

摘要： Mycobacterium tuberculosis proteins and protein compositions that are components of a desaturase complex are provided. The Mycobacterium tuberculosis desaturase complex may include a desaturase and an oxidoreductase. The complex may include the rv3229c and rv3230c gene products of Mycobacterium tuberculosis. Vectors for expressing the desaturase and the oxidoreductase can be packaged together, including a label that indicates their use as a complex for analyzing desaturation of fatty acids. In addition, methods for screening target ligands specific for a desaturase complex are also provided.

摘要翻译： 提供了结核分枝杆菌蛋白质和作为去饱和酶复合物成分的蛋白质组合物。 结核分枝杆菌去饱和酶复合物可包括去饱和酶和氧化还原酶。 复合物可能包括结核分枝杆菌的rv3229c和rv3230c基因产物。 用于表达去饱和酶和氧化还原酶的载体可以包装在一起，包括指示它们用作分析脂肪酸去饱和的复合物的标签。 此外，还提供了用于筛选去饱和酶复合物特异性的靶配体的方法。

公开(公告)号：US20080182249A1

公开(公告)日：2008-07-31

申请号：US11899081

申请日：2007-09-04

申请人： Brian G. Fox ,  Pablo Sobrado ,  Yong Chang

发明人： Brian G. Fox ,  Pablo Sobrado ,  Yong Chang

IPC分类号： C12Q1/68 ,  C12N15/63

CPC分类号： C12N9/0083 ,  C12N9/004 ,  C12Y114/19001

摘要： Expression system for components of a desaturase complex is provided. The system includes expression of a desaturase and an oxidoreductase. The system may be used for expression of mycobacterial desaturases or for expression of mammalian desaturases. The system may further include cell-free expression of other components of the desaturase complex. The expression system may include expression of stearoyl-CoA. The expression system may further include expression of cytochrome b5. The expression system may also include expression of cytochrome b5 reductase. The expression system may also include expression of Rv3230c. In addition, methods for assaying the activity of a stearoyl-CoA desaturase in vitro are provided.

摘要翻译： 提供了一种去饱和酶复合物组分的表达系统。 该系统包括去饱和酶和氧化还原酶的表达。 该系统可用于表达分枝杆菌去饱和酶或用于哺乳动物去饱和酶的表达。 该系统可以进一步包括去饱和酶复合物的其他组分的无细胞表达。 表达系统可以包括硬脂酰辅酶A的表达。 表达系统还可以包括细胞色素b5的表达。 表达系统还可以包括细胞色素b5还原酶的表达。 表达系统还可以包括Rv3230c的表达。 另外，提供了用于测定硬脂酰辅酶A去饱和酶体外活性的方法。

公开(公告)号：US08268581B2

公开(公告)日：2012-09-18

申请号：US12431315

申请日：2009-04-28

申请人： Brian G. Fox ,  Yong Chang ,  Gary A. Wesenberg ,  Craig A. Bingman

发明人： Brian G. Fox ,  Yong Chang ,  Gary A. Wesenberg ,  Craig A. Bingman

IPC分类号： C12P21/06 ,  C07K14/00 ,  C07H21/04

CPC分类号： C12N9/0071 ,  C07K2319/00 ,  C07K2319/21 ,  C07K2319/31 ,  C07K2319/41 ,  C07K2319/60 ,  C12Y114/19001

摘要： Compositions and methods are provided that are useful for predicting and controlling the stability of expressed polypeptides. The compositions and methods may be used to predict and as desired, increase or decrease the stability of proteins recombinantly expressed in mycobacteria, for example DesA3 expressed in Mycobacterium smegmatis. At the C terminus and the penultimate position, substitution to residues with charged side chains, large non-polar side chains, or no side chains can be used to reduce or inhibit the protein degradation. At the antepenultimate position from the C terminus, residues with no side chain or acidic side chains can increase the stability, i.e. reduce or inhibit the protein degradation. The combinational substitution of only the last three residues of polypeptides can make the polypeptides more stable during heterologous expression in mycobacterial hosts.

摘要翻译： 提供了可用于预测和控制表达多肽的稳定性的组合物和方法。 组合物和方法可以用于预测和按需要增加或降低在分枝杆菌中重组表达的蛋白质的稳定性，例如在耻垢分枝杆菌中表达的DesA3。 在C末端和倒数第二的位置，可以使用带有带侧链，大非极性侧链或无侧链的残基取代以降低或抑制蛋白质降解。 在C末端的最初位置，没有侧链或酸性侧链的残基可以增加稳定性，即降低或抑制蛋白质降解。 仅在多肽的最后3个残基的组合取代可以使多肽在分枝杆菌宿主的异源表达期间更稳定。

公开(公告)号：US07601358B2

公开(公告)日：2009-10-13

申请号：US11899080

申请日：2007-09-04

申请人： Brian G. Fox ,  Yong Chang

发明人： Brian G. Fox ,  Yong Chang

IPC分类号： A61K39/04 ,  A61K39/38 ,  A61K39/00

CPC分类号： C12N9/0083 ,  C07K2319/21 ,  C07K2319/50 ,  C12N9/004

摘要： Mycobacterium tuberculosis proteins and protein compositions that are components of a desaturase complex are provided. The Mycobacterium tuberculosis desaturase complex may include a desaturase and an oxidoreductase. The complex may include the rv3229c and rv3230c gene products of Mycobacterium tuberculosis. Vectors for expressing the desaturase and the oxidoreductase can be packaged together, including a label that indicates their use as a complex for analyzing desaturation of fatty acids. In addition, methods for screening target ligands specific for a desaturase complex are also provided.

摘要翻译： 提供了结核分枝杆菌蛋白质和作为去饱和酶复合物成分的蛋白质组合物。 结核分枝杆菌去饱和酶复合物可包括去饱和酶和氧化还原酶。 复合物可能包括结核分枝杆菌的rv3229c和rv3230c基因产物。 用于表达去饱和酶和氧化还原酶的载体可以包装在一起，包括指示它们用作分析脂肪酸去饱和的复合物的标签。 此外，还提供了用于筛选去饱和酶复合物特异性的靶配体的方法。

公开(公告)号：US09508105B2

公开(公告)日：2016-11-29

申请号：US13253668

申请日：2011-10-05

申请人： Dong-Keon Kong ,  Joon-Ho Park ,  Yong Chang ,  Hyoung-Kyu Lim ,  Se-Hee Han

发明人： Dong-Keon Kong ,  Joon-Ho Park ,  Yong Chang ,  Hyoung-Kyu Lim ,  Se-Hee Han

IPC分类号： G06F1/26 ,  G06Q50/06 ,  G05B15/02 ,  H04L12/28 ,  H02J3/14

CPC分类号： G06Q50/06 ,  G05B15/02 ,  H02J2003/143 ,  H04L12/28 ,  H04L12/2829 ,  H04L2012/285 ,  Y02B70/325 ,  Y04S20/228

摘要： A method for configuring a Home Energy Management System (HEMS) in a Home Area Network (HAN) is provided. The method includes registering the HEMS to a smart meter, and managing an energy profile of an HAN device including the smart meter connected to the HAN. Accordingly, a Demand Response (DR) may not normally operate between devices even if energy profile differs from one device to another.

摘要翻译： 提供了一种在家庭网络（HAN）中配置家庭能源管理系统（HEMS）的方法。 该方法包括将HEMS注册到智能电表，以及管理包括连接到HAN的智能电表的HAN设备的能量曲线。 因此，即使能量分布不同于一个设备，需求响应（DR）也不能在设备之间正常工作。

公开(公告)号：US09097103B2

公开(公告)日：2015-08-04

申请号：US13126540

申请日：2009-10-28

申请人： Julian J. Pop ,  Yong Chang

发明人： Julian J. Pop ,  Yong Chang

IPC分类号： G06G7/48 ,  G06G7/50 ,  E21B49/08

CPC分类号： E21B49/08 ,  E21B21/08 ,  G05B13/048

摘要： Methods and apparatus for planning and dynamically updating sampling operations while drilling in a subterranean formation are described. An example method of planning a sampling while drilling operation for a subterranean formation includes identifying a plurality of processes and related parameters, the processes including drilling and sampling processes and the related parameters including drilling and sampling parameters. The example method also involves processing the parameters for each of the processes via a simulation engine to generate predictions associated with sampling the formation, the simulation engine including at least one of a wellbore hydraulics simulator, a mudcake simulator, a formation flow simulator, or a tool response simulator. The example method also involves ranking the predictions associated with sampling the formation based on at least one of a sample fluid quality, a sampling process duration, a sampling process efficiency or a cost of sampling, and planning the sampling operation based on the ranked predictions.

摘要翻译： 描述了在地层中钻孔时规划和动态更新取样操作的方法和装置。 在对地下地层进行钻井操作时规划采样的示例性方法包括识别多个过程和相关参数，所述过程包括钻井和采样过程以及相关参数，包括钻井和采样参数。 该示例性方法还涉及通过模拟引擎处理每个过程的参数以产生与采样地层相关联的预测，所述模拟引擎包括井筒液压模拟器，泥饼模拟器，地层流动模拟器或 工具响应模拟器。 该示例性方法还包括基于样本流体质量，采样过程持续时间，采样过程效率或采样成本中的至少一个以及基于排序的预测来规划采样操作中的至少一个来对与地层采样相关联的预测进行排序。

公开(公告)号：US08656480B2

公开(公告)日：2014-02-18

申请号：US11908932

申请日：2006-02-14

申请人： Seok-Heon Cho ,  Tae-Yong Lee ,  Sun-Hwa Lim ,  Chul-Sik Yoon ,  Jun-Hyuk Song ,  Ji-Cheol Lee ,  Yong Chang

发明人： Seok-Heon Cho ,  Tae-Yong Lee ,  Sun-Hwa Lim ,  Chul-Sik Yoon ,  Jun-Hyuk Song ,  Ji-Cheol Lee ,  Yong Chang

IPC分类号： H04L29/06

CPC分类号： H04L9/3242 ,  H04L63/205 ,  H04L2209/80

摘要： The present invention relates to a subscriber station security-related parameter negotiation method in a wireless portable Internet system. The subscriber station security-related parameter negotiation method includes security-related parameters in transmitting/receiving basic capability negotiation request messages and basic capability negotiation response messages such that the subscriber station and the base station negotiate the subscriber station security-related parameters. The security-related parameters include an authorization policy support subfield used to negotiate an authorization policy between the subscriber station and the base station, and message authentication code mode subfields used to negotiate a message authentication code mode. The base station can inform the subscriber station that authentication or message authentication is not performed and is omitted according to a service provider policy by writing it on the authorization policy support subfield or message authentication code mode subfield. In addition, the subscriber station and the base station can select an authorization policy formed with at least one combination through the authorization policy support subfield of the security negotiation parameters. According to the present invention, the service provider of the wireless portable Internet system can more efficiently and flexibly manage the system by providing a scheme for omitting an authentication function and a message authentication function as well as for supporting various authorization policies and message authentication functions.

摘要翻译： 本发明涉及无线便携式因特网系统中的用户台安全相关参数协商方法。 用户站安全相关参数协商方法包括发送/接收基本能力协商请求消息中的安全相关参数和基本能力协商响应消息，使得用户台和基站协商用户站安全相关参数。 安全相关参数包括用于在用户站和基站之间协商授权策略的授权策略支持子字段以及用于协商消息认证码模式的消息认证码模式子字段。 基站可以通知订户站认证或消息认证不被执行，并且根据服务提供商策略将其写入授权策略支持子字段或消息认证码模式子字段而被省略。 此外，用户站和基站可以通过安全协商参数的授权策略支持子域，选择通过至少一个组合形成的授权策略。 根据本发明，通过提供省略认证功能和消息认证功能以及支持各种授权策略和消息认证功能的方案，无线便携式因特网系统的服务提供商能够更有效和灵活地管理系统。

公开(公告)号：US08288302B2

公开(公告)日：2012-10-16

申请号：US11629375

申请日：2005-06-23

申请人： Kyung Byung Yoon ,  Jin Seok Lee ,  Kwang Ha ,  Yun-Jo Lee ,  Yong Chang

发明人： Kyung Byung Yoon ,  Jin Seok Lee ,  Kwang Ha ,  Yun-Jo Lee ,  Yong Chang

IPC分类号： B01J29/06

CPC分类号： C23C22/02 ,  B82Y15/00 ,  C03C17/002 ,  C03C17/007 ,  C03C17/009 ,  C03C2217/23 ,  C03C2217/465 ,  C03C2217/475 ,  C23C26/00 ,  C23C30/00

摘要： The present invention relates to a method for preparing substrate-molecular sieve layer complex by vising ultra-sound and apparatuses used therein, more particularly to a method for preparing substrate-molecular sieve layer complex by combining substrate, coupling compound and molecular sieve particle, wherein covalent, ionic, coordinate or hydrogen bond between a substrate and a coupling compound; molecular sieve particle and coupling compound; coupling compounds; coupling compound and intermediate coupling compound is induced by using 15 KHz-100 MHz of ultrasound instead of simple reflux to combine substrate and molecular sieve particles by various processes, further to reduce time and energy, to retain high binding velocity, binding strength, binding intensity and density remarkably, to attach molecular sieve particle uniformly onto all substrates combined with coupling compound selectively, even though substrate with coupling compound and substrate without coupling compound exist together; and apparatuses installed therein, which can improve to produce substrate-molecular sieve layer complex in a large scale.

摘要翻译： 本发明涉及一种通过超声波和其中使用的装置制备底物 - 分子筛层复合物的方法，更具体地涉及一种通过将底物，偶联化合物和分子筛颗粒组合制备底物 - 分子筛层复合物的方法，其中 底物和偶联化合物之间的共价，离子，配位或氢键; 分子筛颗粒和偶联剂; 偶联化合物; 通过使用15KHz-100MHz的超声波而不是简单回流来诱导偶联化合物和中间偶联化合物，以通过各种方法将底物和分子筛颗粒结合在一起，进一步减少时间和能量，以保持高结合速度，结合强度，结合强度 和密度显着地将分子筛颗粒均匀地附着在与偶联化合物组合的所有基材上，即使具有偶联化合物的底物和没有偶联化合物的底物一起存在; 以及安装在其中的装置，其可以改进以大规模生产基质 - 分子筛层复合物。

公开(公告)号：US20120253679A1

公开(公告)日：2012-10-04

申请号：US13069674

申请日：2011-03-23

申请人： Yong Chang ,  Julian J. Pop

发明人： Yong Chang ,  Julian J. Pop

IPC分类号： G06F19/00 ,  E21B49/10

CPC分类号： E21B49/008 ,  E21B49/088

摘要： Methods of and apparatus to perform a drawdown of a formation fluid in a downhole environment are disclosed. An example method includes contacting a borehole wall with a fluid communication device of a formation testing tool and performing a first type of drawdown to draw fluid into the fluid communication device. The method also includes detecting a breach of a mudcake on the borehole wall during performance of the first type of drawdown and performing a second type of drawdown to draw fluid into the sample probe in response to detecting the breach of the mudcake. The second type of drawdown is different than the first type of drawdown. Furthermore, the example method includes confirming the breach of the mudcake on the borehole wall during performance of the second type of drawdown.

摘要翻译： 公开了在井下环境中进行地层流体的压下的方法和装置。 一个示例性方法包括使钻孔壁与地层测试工具的流体连通装置接触并执行第一类型的抽出以将流体引入到流体通信装置中。 该方法还包括在执行第一类型的抽出期间检测在井壁上的泥饼的破裂，并且响应于检测到泥饼的破裂而执行第二类型的抽出以将流体抽入样品探针。 第二种缩减方式不同于第一种缩减方式。 此外，示例性方法包括在执行第二种类型的抽出期间确认在井壁上的泥饼的破裂。