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    Porous and Non-Porous Cell Culture Substrate
    7.
    发明申请
    Porous and Non-Porous Cell Culture Substrate 审中-公开
    多孔和非多孔细胞培养基质

    公开(公告)号：US20120052565A1

    公开(公告)日：2012-03-01

    申请号：US13218103

    申请日：2011-08-25

    申请人： Michael Edward DeRosa ,  Joydeep Lahiri ,  Hongwei Hanna Rao ,  Po Ki Yuen

    发明人： Michael Edward DeRosa ,  Joydeep Lahiri ,  Hongwei Hanna Rao ,  Po Ki Yuen

    IPC分类号： C12M3/00 ,  C23F1/00

    CPC分类号： C08J7/02 ,  C08J2323/08 ,  C08J2325/06 ,  C12M23/22 ,  C12M25/14

    摘要： A cell culture article has substrate that is predominantly opaque and that provides a three-dimensional (e.g., irregular) surface, but incorporates an optically transparent, substantially regular (e.g., two-dimensional) surface to serve as a microscopic observation and imaging window for the 3D cell culture. In many embodiments, the 3D portion of the substrate occupies greater than 99% of the surface while the 2D portion occupies less than 1% of the surface so as not to substantially disrupt the general 3D culture environment.

    摘要翻译： 细胞培养物具有主要是不透明的并且提供三维（例如，不规则）表面的基底，但是包含光学透明的，基本上规则的（例如，二维）表面，用作微观观察和成像窗口 3D细胞培养。 在许多实施例中，基板的3D部分占据表面的大于99％，而2D部分占据表面的1％以下，以免基本上破坏一般的3D文化环境。

    System and method for performing G protein coupled receptor (GPCR) cell assays using waveguide-grating sensors
    8.
    发明授权
    System and method for performing G protein coupled receptor (GPCR) cell assays using waveguide-grating sensors 有权
    使用波导光栅传感器进行G蛋白偶联受体（GPCR）细胞测定的系统和方法

    公开(公告)号：US08148092B2

    公开(公告)日：2012-04-03

    申请号：US12469054

    申请日：2009-05-20

    申请人： Ye Fang ,  Ann M. Ferrie ,  Norman H. Fontaine ,  Joydeep Lahiri ,  Po Ki Yuen

    发明人： Ye Fang ,  Ann M. Ferrie ,  Norman H. Fontaine ,  Joydeep Lahiri ,  Po Ki Yuen

    IPC分类号： G01N33/567 ,  C12Q1/02

    CPC分类号： G01N33/5041 ,  G01N21/4788 ,  G01N21/553 ,  G01N2333/726 ,  G01N2500/10

    摘要： The present invention includes a system and method that uses optical LID biosensors to monitor in real time agonist-induced GPCR signaling events within living cells. Particularly, the present invention includes a system and method for using an optical LID biosensor to screen compounds against a target GPCR within living cells based on the mass redistribution due to agonist-induced GPCR activation. In an extended embodiment, the present invention discloses different ways for self-referencing the optical LID biosensor to eliminate unwanted sensitivity to ambient temperature, pressure fluctuations, and other environmental changes. In yet another extended embodiment, the present invention discloses different ways for screening multiple GPCRs in a single type of cell or multiple GPCRs in multiple types of cells within a single medium solution. In still yet another extended embodiment, the present invention discloses different ways to confirm the physiological or pharmacological effect of a compound against a specific GPCR within living cells.

    摘要翻译： 本发明包括使用光学LID生物传感器实时监测活细胞内激动剂诱导的GPCR信号事件的系统和方法。 特别地，本发明包括使用光学LID生物传感器基于由于激动剂诱导的GPCR活化导致的质量再分布来筛选活细胞内的靶GPCR的化合物的系统和方法。 在扩展实施例中，本发明公开了用于自参考光学LID生物传感器以消除对环境温度，压力波动和其他环境变化的不期望的灵敏度的不同方式。 在又一个扩展的实施方案中，本发明公开了在单一培养基溶液中的多种类型的细胞中单种类型细胞或多种GPCR筛选多种GPCR的不同方式。 在又一个延伸的实施方案中，本发明公开了确定化合物对活细胞内特异性GPCR的生理或药理作用的不同方式。

    System and Method for Performing G Protein Coupled Receptor (GPCR) Cell Assays Using Waveguide-Grating Sensors
    10.
    发明申请
    System and Method for Performing G Protein Coupled Receptor (GPCR) Cell Assays Using Waveguide-Grating Sensors 有权
    使用波导光栅传感器进行G蛋白偶联受体（GPCR）细胞测定的系统和方法

    公开(公告)号：US20090275074A1

    公开(公告)日：2009-11-05

    申请号：US12469054

    申请日：2009-05-20

    申请人： Ye Fang ,  Ann M. Ferrie ,  Norman H. Fontaine ,  Joydeep Lahiri ,  Po Ki Yuen

    发明人： Ye Fang ,  Ann M. Ferrie ,  Norman H. Fontaine ,  Joydeep Lahiri ,  Po Ki Yuen

    IPC分类号： C12Q1/02

    CPC分类号： G01N33/5041 ,  G01N21/4788 ,  G01N21/553 ,  G01N2333/726 ,  G01N2500/10

    摘要： The present invention includes a system and method that uses optical LID biosensors to monitor in real time agonist-induced GPCR signaling events within living cells. Particularly, the present invention includes a system and method for using an optical LID biosensor to screen compounds against a target GPCR within living cells based on the mass redistribution due to agonist-induced GPCR activation. In an extended embodiment, the present invention discloses different ways for self-referencing the optical LID biosensor to eliminate unwanted sensitivity to ambient temperature, pressure fluctuations, and other environmental changes. In yet another extended embodiment, the present invention discloses different ways for screening multiple GPCRs in a single type of cell or multiple GPCRs in multiple types of cells within a single medium solution. In still yet another extended embodiment, the present invention discloses different ways to confirm the physiological or pharmacological effect of a compound against a specific GPCR within living cells.

    摘要翻译： 本发明包括使用光学LID生物传感器实时监测活细胞内激动剂诱导的GPCR信号事件的系统和方法。 特别地，本发明包括使用光学LID生物传感器基于由于激动剂诱导的GPCR活化导致的质量再分布来筛选活细胞内的靶GPCR的化合物的系统和方法。 在扩展实施例中，本发明公开了用于自参考光学LID生物传感器以消除对环境温度，压力波动和其他环境变化的不期望的灵敏度的不同方式。 在又一个扩展的实施方案中，本发明公开了在单一培养基溶液中的多种类型的细胞中单种类型细胞或多种GPCR筛选多种GPCR的不同方式。 在又一个延伸的实施方案中，本发明公开了确定化合物对活细胞内特异性GPCR的生理或药理作用的不同方式。