公开(公告)号：US20120021490A1

公开(公告)日：2012-01-26

申请号：US13156538

申请日：2011-06-09

申请人： Brian Steer ,  Walter Callen ,  Shaun Healey ,  Derrick Pulliam

发明人： Brian Steer ,  Walter Callen ,  Shaun Healey ,  Derrick Pulliam

IPC分类号： C12N9/42 ,  C12N15/63 ,  C40B50/00 ,  C12N5/10 ,  C12N1/19 ,  C12N1/15 ,  C12N15/56 ,  C12N1/21

CPC分类号： C12N9/2405 ,  A23C9/1322 ,  A23C19/04 ,  A23K10/14 ,  A23K20/189 ,  A23K40/10 ,  A23K50/10 ,  A23L2/52 ,  A23L29/10 ,  A23L33/18 ,  A23V2002/00 ,  A61K9/0056 ,  A61K38/47 ,  C09K8/62 ,  C11D3/38636 ,  C12N9/2437 ,  C12N11/02 ,  C12N11/14 ,  C12N11/16 ,  C12P7/10 ,  C12P19/02 ,  C12P19/18 ,  C12Y302/01004 ,  D21C1/00 ,  D21C5/005 ,  G01N33/53 ,  G01N33/573 ,  G01N2333/924 ,  G01N2500/00 ,  Y02E50/16 ,  Y02E50/17

摘要： The invention relates to polypeptides having glucanase, e.g., endoglucanase, mannanase, xylanase activity or a combination of these activities, and polynucleotides encoding them. In one aspect, the glucanase activity is an endoglucanase activity (e.g., endo-1,4-beta-D-glucan 4-glucano hydrolase activity) and comprises hydrolysis of 1,4-beta-D-glycosidic linkages in cellulose, cellulose derivatives (e.g., carboxy methyl cellulose and hydroxy ethyl cellulose) lichenin, beta-1,4 bonds in mixed beta-1,3 glucans, such as cereal beta-D-glucans or xyloglucans and other plant material containing cellulosic parts. In addition, methods of designing new enzymes and methods of use thereof are also provided. In alternative aspects, the new glucanases e.g., endoglucanases, mannanases, xylanases have increased activity and stability at increased pH and temperature.

摘要翻译： 本发明涉及具有葡聚糖酶的多肽，例如内切葡聚糖酶，甘露聚糖酶，木聚糖酶活性或这些活性的组合，以及编码它们的多核苷酸。 一方面，葡聚糖酶活性是内切葡聚糖酶活性（例如内切-1,4-β-D-葡聚糖4-葡聚糖水解酶活性），并且包括在纤维素，纤维素衍生物中水解1,4-β-D-糖苷键 （例如，羧甲基纤维素和羟乙基纤维素）地衣淀粉，混合的β-1,3葡聚糖中的β-1,4键，例如谷类β-D-葡聚糖或木葡聚糖和含有纤维素部分的其它植物材料。 此外，还提供了设计新酶的方法及其使用方法。 在替代方面，新葡聚糖酶例如内切葡聚糖酶，甘露聚糖酶，木聚糖酶在增加的pH和温度下具有增加的活性和稳定性。

公开(公告)号：US20080233175A1

公开(公告)日：2008-09-25

申请号：US10560957

申请日：2004-07-02

申请人： Brian Steer ,  Walter Callen ,  Shaun Healey ,  Derrick Pulliam

发明人： Brian Steer ,  Walter Callen ,  Shaun Healey ,  Derrick Pulliam

IPC分类号： A61K47/00

CPC分类号： C12N9/2405 ,  A23C9/1322 ,  A23C19/04 ,  A23K10/14 ,  A23K20/189 ,  A23K40/10 ,  A23K50/10 ,  A23L2/52 ,  A23L29/10 ,  A23L33/18 ,  A23V2002/00 ,  A61K9/0056 ,  A61K38/47 ,  C09K8/62 ,  C11D3/38636 ,  C12N9/2437 ,  C12N11/02 ,  C12N11/14 ,  C12N11/16 ,  C12P7/10 ,  C12P19/02 ,  C12P19/18 ,  C12Y302/01004 ,  D21C1/00 ,  D21C5/005 ,  G01N33/53 ,  G01N33/573 ,  G01N2333/924 ,  G01N2500/00 ,  Y02E50/16 ,  Y02E50/17

摘要： The invention relates to polypeptides having glucanase, e.g., endoglucanase, mannanase, xylanase activity or a combination of these activities, and polynucleotides encoding them. In one aspect, the glucanase activity is an endoglucanase activity (e.g., endo-1,4-beta-D-glucan 4-glucano hydrolase activity) and comprises hydrolysis of 1,4-beta-D-glycosidic linkages in cellulose, cellulose derivatives (e.g., carboxy methyl cellulose and hydroxy ethyl cellulose) lichenin, beta-1,4 bonds in mixed beta-1,3 glucans, such as cereal beta-D-glucans or xyloglucans and other plant material containing cellulosic parts. In addition, methods of designing new enzymes and methods of use thereof are also provided. In alternative aspects, the new glucanases e.g., endoglucanases, mannanases, xylanases have increased activity and stability at increased pH and temperature.

摘要翻译： 本发明涉及具有葡聚糖酶的多肽，例如内切葡聚糖酶，甘露聚糖酶，木聚糖酶活性或这些活性的组合，以及编码它们的多核苷酸。 一方面，葡聚糖酶活性是内切葡聚糖酶活性（例如内切-1,4-β-D-葡聚糖4-葡聚糖水解酶活性），并且包括在纤维素，纤维素衍生物中水解1,4-β-D-糖苷键 （例如，羧甲基纤维素和羟乙基纤维素）地衣淀粉，混合的β-1,3葡聚糖中的β-1,4键，例如谷类β-D-葡聚糖或木葡聚糖和含有纤维素部分的其它植物材料。 此外，还提供了设计新酶的方法及其使用方法。 在替代方面，新葡聚糖酶例如内切葡聚糖酶，甘露聚糖酶，木聚糖酶在增加的pH和温度下具有增加的活性和稳定性。

公开(公告)号：US07960148B2

公开(公告)日：2011-06-14

申请号：US10560957

申请日：2004-07-02

申请人： Brian Steer ,  Walter Callen ,  Shaun Healey ,  Derrick Pulliam

发明人： Brian Steer ,  Walter Callen ,  Shaun Healey ,  Derrick Pulliam

IPC分类号： C12P19/18 ,  C12P7/06 ,  C12N9/42 ,  A61K47/00

CPC分类号： C12N9/2405 ,  A23C9/1322 ,  A23C19/04 ,  A23K10/14 ,  A23K20/189 ,  A23K40/10 ,  A23K50/10 ,  A23L2/52 ,  A23L29/10 ,  A23L33/18 ,  A23V2002/00 ,  A61K9/0056 ,  A61K38/47 ,  C09K8/62 ,  C11D3/38636 ,  C12N9/2437 ,  C12N11/02 ,  C12N11/14 ,  C12N11/16 ,  C12P7/10 ,  C12P19/02 ,  C12P19/18 ,  C12Y302/01004 ,  D21C1/00 ,  D21C5/005 ,  G01N33/53 ,  G01N33/573 ,  G01N2333/924 ,  G01N2500/00 ,  Y02E50/16 ,  Y02E50/17

摘要： The invention relates to polypeptides having glucanase, e.g., endoglucanase, mannanase, xylanase activity or a combination of these activities, and polynucleotides encoding them. In one aspect, the glucanase activity is an endoglucanase activity (e.g., endo-1,4-beta-D-glucan 4-glucano hydrolase activity) and comprises hydrolysis of 1,4-beta-D-glycosidic linkages in cellulose, cellulose derivatives (e.g., carboxy methyl cellulose and hydroxy ethyl cellulose) lichenin, beta-1,4 bonds in mixed beta-1,3 glucans, such as cereal beta-D-glucans or xyloglucans and other plant material containing cellulosic parts. In addition, methods of designing new enzymes and methods of use thereof are also provided. In alternative aspects, the new glucanases e.g., endoglucanases, mannanases, xylanases have increased activity and stability at increased pH and temperature.

摘要翻译： 本发明涉及具有葡聚糖酶的多肽，例如内切葡聚糖酶，甘露聚糖酶，木聚糖酶活性或这些活性的组合，以及编码它们的多核苷酸。 一方面，葡聚糖酶活性是内切葡聚糖酶活性（例如内切-1,4-β-D-葡聚糖4-葡聚糖水解酶活性），并且包括在纤维素，纤维素衍生物中水解1,4-β-D-糖苷键 （例如，羧甲基纤维素和羟乙基纤维素）地衣淀粉，混合的β-1,3葡聚糖中的β-1,4键，例如谷类β-D-葡聚糖或木葡聚糖和含有纤维素部分的其它植物材料。 此外，还提供了设计新酶的方法及其使用方法。 在替代方面，新葡聚糖酶例如内切葡聚糖酶，甘露聚糖酶，木聚糖酶在增加的pH和温度下具有增加的活性和稳定性。

公开(公告)号：US08618272B2

公开(公告)日：2013-12-31

申请号：US13534202

申请日：2012-06-27

申请人： Nykoll Long ,  Jeffrey Bottoms ,  Moez Rajabali Meghji ,  Hope Hart ,  Qiudeng Que ,  Derrick Pulliam

发明人： Nykoll Long ,  Jeffrey Bottoms ,  Moez Rajabali Meghji ,  Hope Hart ,  Qiudeng Que ,  Derrick Pulliam

IPC分类号： C12N15/11 ,  C12N15/32

CPC分类号： C12Q1/6895 ,  C07K14/415 ,  C12N15/8286 ,  C12Q2600/13 ,  Y02A40/162

摘要： A novel transgenic corn event designated MIR162 is disclosed. The invention relates to nucleic acids that are unique to event MIR162 and to methods for detecting the presence of the MIR162 event based on DNA sequences of the recombinant constructs inserted into the corn genome that resulted in the MIR162 event and of genomic sequences flanking the insertion site. The invention further relates to corn plants comprising the transgenic genotype of MIR162 and to methods for producing a corn plant by crossing a corn plant comprising the MIR162 genotype with itself or another corn variety. Seeds of corn plants comprising the MIR162 genotype are also objects of the present invention. The invention also relates to methods of controlling insects using MIR162 corn plants.

摘要翻译： 公开了一种称为MIR162的新型转基因玉米事件。 本发明涉及对事件MIR162独特的核酸和基于插入到导致MIR162事件的玉米基因组中的重组构建体的DNA序列以及侧翼于插入位点的基因组序列的MIR162事件的存在的方法 。 本发明进一步涉及包含MIR162的转基因基因型的玉米植物，以及通过将包含MIR162基因型的玉米植物与其本身或另一种玉米品种杂交来生产玉米植物的方法。 包含MIR162基因型的玉米植物种子也是本发明的目的。 本发明还涉及使用MIR162玉米植物控制昆虫的方法。

公开(公告)号：US20120272402A1

公开(公告)日：2012-10-25

申请号：US13534231

申请日：2012-06-27

申请人： Nykoll Long ,  Jeff Bottoms ,  Moez Meghji ,  Hope Hart ,  Qiudeng Que ,  Derrick Pulliam

发明人： Nykoll Long ,  Jeff Bottoms ,  Moez Meghji ,  Hope Hart ,  Qiudeng Que ,  Derrick Pulliam

IPC分类号： C12N15/82 ,  C07H21/04

CPC分类号： C12Q1/6895 ,  C07K14/415 ,  C12N15/8286 ,  C12Q2600/13 ,  Y02A40/162

摘要： A novel transgenic corn event designated MIR162 is disclosed. The invention relates to nucleic acids that are unique to event MIR162 and to methods for detecting the presence of the MIR162 event based on DNA sequences of the recombinant constructs inserted into the corn genome that resulted in the MIR162 event and of genomic sequences flanking the insertion site. The invention further relates to corn plants comprising the transgenic genotype of MIR162 and to methods for producing a corn plant by crossing a corn plant comprising the MIR162 genotype with itself or another corn variety. Seeds of corn plants comprising the MIR162 genotype are also objects of the present invention. The invention also relates to methods of controlling insects using MIR162 corn plants.

公开(公告)号：US08232456B2

公开(公告)日：2012-07-31

申请号：US12301824

申请日：2007-05-24

申请人： Nykoll Long ,  Jeff Bottoms ,  Moez Meghji ,  Hope Hart ,  Qiudeng Que ,  Derrick Pulliam

发明人： Nykoll Long ,  Jeff Bottoms ,  Moez Meghji ,  Hope Hart ,  Qiudeng Que ,  Derrick Pulliam

IPC分类号： C12N15/82 ,  A01H1/00

CPC分类号： C12Q1/6895 ,  C07K14/415 ,  C12N15/8286 ,  C12Q2600/13 ,  Y02A40/162

摘要： A novel transgenic corn event designated MIR162 is disclosed. The invention relates to nucleic acids from event MIR162. The invention also relates to assays for detecting the presence of the MIR162 event based on DNA sequences of the recombinant constructs inserted into the corn genome that resulted in the MIR162 event and of genomic sequences flanking the insertion sites. The invention further relates to corn plants comprising the genotype of MIR162 and to methods for producing a corn plant by crossing a corn plant comprising the MIR162 genotype with itself or another corn variety. Seeds of corn plants comprising the MIR162 genotype are also objects of the present invention. The invention also relates to methods of controlling insects using MIR162 corn plants.

摘要翻译： 公开了一种称为MIR162的新型转基因玉米事件。 本发明涉及事件MIR162的核酸。 本发明还涉及基于导入MIR162事件的插入到玉米基因组中的重组构建体的DNA序列和在插入位点侧翼的基因组序列来检测MIR162事件的存在的测定。 本发明还涉及包含MIR162的基因型的玉米植物和通过将包含MIR162基因型的玉米植物与其本身或另一种玉米品种杂交来生产玉米植物的方法。 包含MIR162基因型的玉米植物种子也是本发明的目的。 本发明还涉及使用MIR162玉米植物控制昆虫的方法。

公开(公告)号：US20120167260A1

公开(公告)日：2012-06-28

申请号：US13421920

申请日：2012-03-16

申请人： Nykoll Long ,  Jeff Bottoms ,  Moez Meghji ,  Hope Hart ,  Qiudeng Que ,  Derrick Pulliam

发明人： Nykoll Long ,  Jeff Bottoms ,  Moez Meghji ,  Hope Hart ,  Qiudeng Que ,  Derrick Pulliam

IPC分类号： A01C1/06 ,  A01H5/00

CPC分类号： C12Q1/6895 ,  C07K14/415 ,  C12N15/8286 ,  C12Q2600/13 ,  Y02A40/162

摘要： A novel transgenic corn event designated MIR162 is disclosed. The invention relates to nucleic acids that are unique to event MIR162 and to methods for detecting the presence of the MIR162 event based on DNA sequences of the recombinant constructs inserted into the corn genome that resulted in the MIR162 event and of genomic sequences flanking the insertion site. The invention further relates to corn plants comprising the transgenic genotype of MIR162 and to methods for producing a corn plant by crossing a corn plant comprising the MIR162 genotype with itself or another corn variety. Seeds of corn plants comprising the MIR162 genotype are also objects of the present invention. The invention also relates to methods of controlling insects using MIR162 corn plants.

公开(公告)号：US20050208178A1

公开(公告)日：2005-09-22

申请号：US11018645

申请日：2004-12-20

申请人： Michael Bauer ,  Michael Bedford ,  Derrick Pulliam

发明人： Michael Bauer ,  Michael Bedford ,  Derrick Pulliam

IPC分类号： A23B7/10 ,  A23K1/00 ,  A23K1/165 ,  A23K1/18 ,  A23L1/305 ,  C07H21/04 ,  C12N1/18 ,  C12N9/24 ,  C12P21/06 ,  C12Q1/68

CPC分类号： C12N9/2482 ,  A23K20/189 ,  A23K20/20 ,  A23K40/20 ,  A23K40/25 ,  A23K50/10 ,  A23K50/20 ,  A23K50/30 ,  A23K50/75 ,  A23K50/80 ,  A23L33/195 ,  C07K2319/02 ,  C12N9/248 ,  C12Y302/01008 ,  Y02A40/818

摘要： The present invention relates to codon-optimized xylanase coding sequences and the expression of xylanases in microbes and yeast. The invention further relates to using multiple copies of the xylanase expression construct for high levels of protein expression. The invention also relates to the use of xylanases as feed or food additives. The invention also relates to methods of expression of enzymes to increase thermotolerance by expressing them in organisms that glycosylate proteins compared to expression that the same enzyme without the glycosylation. Further, the invention relates to methods of preparing feed, enzyme feed additives, and methods of reducing the feed conversion ration or increasing weight gain of animals.

摘要翻译： 本发明涉及密码子优化的木聚糖酶编码序列和微生物和酵母中木聚糖酶的表达。 本发明还涉及使用木聚糖酶表达构建体的多个拷贝用于高水平的蛋白质表达。 本发明还涉及木聚糖酶作为饲料或食品添加剂的用途。 本发明还涉及通过在与没有糖基化的相同酶的表达相比糖蛋白的生物体中表达它们来增加耐热性来增加耐热性的方法。 此外，本发明涉及制备饲料，酶饲料添加剂的方法以及减少饲料转化率或增加动物体重增加的方法。

公开(公告)号：US08455720B2

公开(公告)日：2013-06-04

申请号：US13421920

申请日：2012-03-16

申请人： Nykoll Long ,  Jeffrey Bottoms ,  Moez Rajabali Meghji ,  Hope Hart ,  Qiudeng Que ,  Derrick Pulliam

发明人： Nykoll Long ,  Jeffrey Bottoms ,  Moez Rajabali Meghji ,  Hope Hart ,  Qiudeng Que ,  Derrick Pulliam

IPC分类号： C12N15/82

CPC分类号： C12Q1/6895 ,  C07K14/415 ,  C12N15/8286 ,  C12Q2600/13 ,  Y02A40/162

摘要： A novel transgenic corn event designated MIR162 is disclosed. The invention relates to nucleic acids that are unique to event MIR162 and to methods for detecting the presence of the MIR162 event based on DNA sequences of the recombinant constructs inserted into the corn genome that resulted in the MIR162 event and of genomic sequences flanking the insertion site. The invention further relates to corn plants comprising the transgenic genotype of MIR162 and to methods for producing a corn plant by crossing a corn plant comprising the MIR162 genotype with itself or another corn variety. Seeds of corn plants comprising the MIR162 genotype are also objects of the present invention. The invention also relates to methods of controlling insects using MIR162 corn plants.

公开(公告)号：US20120264130A1

公开(公告)日：2012-10-18

申请号：US13534202

申请日：2012-06-27

申请人： Nykoll Long ,  Jeff Bottoms ,  Moez Meghji ,  Hope Hart ,  Qiudeng Que ,  Derrick Pulliam

发明人： Nykoll Long ,  Jeff Bottoms ,  Moez Meghji ,  Hope Hart ,  Qiudeng Que ,  Derrick Pulliam

IPC分类号： C12N15/09 ,  C12N15/63 ,  C07K14/00 ,  C12Q1/68 ,  C07H21/00

CPC分类号： C12Q1/6895 ,  C07K14/415 ,  C12N15/8286 ,  C12Q2600/13 ,  Y02A40/162

摘要： A novel transgenic corn event designated MIR162 is disclosed. The invention relates to nucleic acids that are unique to event MIR162 and to methods for detecting the presence of the MIR162 event based on DNA sequences of the recombinant constructs inserted into the corn genome that resulted in the MIR162 event and of genomic sequences flanking the insertion site. The invention further relates to corn plants comprising the transgenic genotype of MIR162 and to methods for producing a corn plant by crossing a corn plant comprising the MIR162 genotype with itself or another corn variety. Seeds of corn plants comprising the MIR162 genotype are also objects of the present invention. The invention also relates to methods of controlling insects using MIR162 corn plants.

摘要翻译： 公开了一种称为MIR162的新型转基因玉米事件。 本发明涉及对事件MIR162独特的核酸和基于插入到导致MIR162事件的玉米基因组中的重组构建体的DNA序列以及侧翼于插入位点的基因组序列的检测MIR162事件的存在的方法 。 本发明进一步涉及包含MIR162的转基因基因型的玉米植物，以及通过将包含MIR162基因型的玉米植物与其本身或另一种玉米品种杂交来生产玉米植物的方法。 包含MIR162基因型的玉米植物种子也是本发明的目的。 本发明还涉及使用MIR162玉米植物控制昆虫的方法。